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- EMDB-6709: Structure of the 70S chloroplast ribosome from spinach -

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Basic information

Entry
Database: EMDB / ID: EMD-6709
TitleStructure of the 70S chloroplast ribosome from spinach
Map data
Sample
  • Complex: Chloroplast 70S ribosome from spinach
    • Complex: chloroplast ribosome 50S large subunit
      • Protein or peptide: x 30 types
      • RNA: x 2 types
    • Complex: chloroplast ribosome 30S small subunit
      • RNA: x 2 types
      • Protein or peptide: x 24 types
KeywordsCryo-EM / ribosome / chloroplast ribosome
Function / homology
Function and homology information


plastid small ribosomal subunit / plastid translation / negative regulation of translational elongation / primary metabolic process / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / plastid / chloroplast stroma / mitochondrial translation / ribosomal small subunit binding ...plastid small ribosomal subunit / plastid translation / negative regulation of translational elongation / primary metabolic process / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / plastid / chloroplast stroma / mitochondrial translation / ribosomal small subunit binding / chloroplast / DNA-templated transcription termination / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / transferase activity / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / response to antibiotic / mRNA binding / mitochondrion / RNA binding
Similarity search - Function
Ribosomal protein PSRP-3/Ycf65 / PSRP-3/Ycf65 superfamily / Plastid and cyanobacterial ribosomal protein (PSRP-3 / Ycf65) / 30S ribosomal protein S31, plant / Ribosomal protein S31e / Ribosomal protein L6, chloroplast / 50S ribosomal protein 5, chloroplastic / Family of unknown function (DUF5323) / Ribosomal protein L32p, plant/cyanobacteria type / Ribosome hibernation promoting factor, long/plastid ...Ribosomal protein PSRP-3/Ycf65 / PSRP-3/Ycf65 superfamily / Plastid and cyanobacterial ribosomal protein (PSRP-3 / Ycf65) / 30S ribosomal protein S31, plant / Ribosomal protein S31e / Ribosomal protein L6, chloroplast / 50S ribosomal protein 5, chloroplastic / Family of unknown function (DUF5323) / Ribosomal protein L32p, plant/cyanobacteria type / Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / Ribosomal protein S1-like / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / 30S ribosomal protein / S1 domain profile. / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / S1 domain / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / RNA recognition motif / RNA recognition motif / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Eukaryotic RNA Recognition Motif (RRM) profile. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / RNA recognition motif domain / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / RNA-binding domain superfamily / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16
Similarity search - Domain/homology
Large ribosomal subunit protein uL3c / Ribosome-binding factor PSRP1, chloroplastic / Large ribosomal subunit protein uL15c / Large ribosomal subunit protein uL18c / Large ribosomal subunit protein bL31c / Small ribosomal subunit protein cS22 / Large ribosomal subunit protein bL27c / Large ribosomal subunit protein uL6c / Large ribosomal subunit protein uL29c / Large ribosomal subunit protein cL38 ...Large ribosomal subunit protein uL3c / Ribosome-binding factor PSRP1, chloroplastic / Large ribosomal subunit protein uL15c / Large ribosomal subunit protein uL18c / Large ribosomal subunit protein bL31c / Small ribosomal subunit protein cS22 / Large ribosomal subunit protein bL27c / Large ribosomal subunit protein uL6c / Large ribosomal subunit protein uL29c / Large ribosomal subunit protein cL38 / Large ribosomal subunit protein bL28c / Small ribosomal subunit protein bS21c / Small ribosomal subunit protein cS23 / Large ribosomal subunit protein bL17c / Small ribosomal subunit protein bTHXc / Large ribosomal subunit protein bL9c / Small ribosomal subunit protein uS17c / Small ribosomal subunit protein uS10c / Large ribosomal subunit protein cL37 alpha / Large ribosomal subunit protein uL4c / Small ribosomal subunit protein uS11c / Small ribosomal subunit protein uS14c / Small ribosomal subunit protein uS19c / Large ribosomal subunit protein uL2cz/uL2cy / Small ribosomal subunit protein uS2c / Large ribosomal subunit protein uL22c / Small ribosomal subunit protein uS3c / Large ribosomal subunit protein uL14c / Small ribosomal subunit protein uS8c / Large ribosomal subunit protein bL36c / Large ribosomal subunit protein uL13c / Small ribosomal subunit protein uS4c / Large ribosomal subunit protein uL16c / Ribosome-binding factor PSRP1, chloroplastic / Large ribosomal subunit protein uL15c / Large ribosomal subunit protein bL35c / Large ribosomal subunit protein bL21c / Large ribosomal subunit protein uL24c / Large ribosomal subunit protein cL37 alpha / Large ribosomal subunit protein bL20c / Large ribosomal subunit protein bL32c / Large ribosomal subunit protein bL33c / Small ribosomal subunit protein bS16c / Small ribosomal subunit protein bS1c / Small ribosomal subunit protein bTHXc / Small ribosomal subunit protein uS12cz/uS12cy / Small ribosomal subunit protein bS21c / Small ribosomal subunit protein uS7cz/uS7cy / Small ribosomal subunit protein uS17c / Small ribosomal subunit protein uS10c / Small ribosomal subunit protein uS13c / Large ribosomal subunit protein bL9c / Large ribosomal subunit protein bL27c / Large ribosomal subunit protein uL3c / Large ribosomal subunit protein uL5c / Large ribosomal subunit protein uL6c / Large ribosomal subunit protein bL17c / Large ribosomal subunit protein uL18c / Large ribosomal subunit protein bL34c / Large ribosomal subunit protein bL28c / Large ribosomal subunit protein uL29c / Large ribosomal subunit protein bL31c / Small ribosomal subunit protein cS22 / Small ribosomal subunit protein uS9c / Small ribosomal subunit protein bS6c alpha / Large ribosomal subunit protein cL38 / Small ribosomal subunit protein cS23 / Large ribosomal subunit protein bL19c / Large ribosomal subunit protein uL23c / Small ribosomal subunit protein uS15c / Small ribosomal subunit protein bS18c / Small ribosomal subunit protein uS5c
Similarity search - Component
Biological speciesSpinacia oleracea (spinach)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsAhmed T / Shi J
Funding support Singapore, 1 items
OrganizationGrant numberCountry
MOEAcRF Tier 1, 2014-T1-001-019 (RG32/14) Singapore
CitationJournal: Nucleic Acids Res / Year: 2017
Title: Unique localization of the plastid-specific ribosomal proteins in the chloroplast ribosome small subunit provides mechanistic insights into the chloroplastic translation.
Authors: Tofayel Ahmed / Jian Shi / Shashi Bhushan /
Abstract: Chloroplastic translation is mediated by a bacterial-type 70S chloroplast ribosome. During the evolution, chloroplast ribosomes have acquired five plastid-specific ribosomal proteins or PSRPs (cS22, ...Chloroplastic translation is mediated by a bacterial-type 70S chloroplast ribosome. During the evolution, chloroplast ribosomes have acquired five plastid-specific ribosomal proteins or PSRPs (cS22, cS23, bTHXc, cL37 and cL38) which have been suggested to play important regulatory roles in translation. However, their exact locations on the chloroplast ribosome remain elusive due to lack of a high-resolution structure, hindering our progress to understand their possible roles. Here we present a cryo-EM structure of the 70S chloroplast ribosome from spinach resolved to 3.4 Å and focus our discussion mainly on the architecture of the 30S small subunit (SSU) which is resolved to 3.7 Å. cS22 localizes at the SSU foot where it seems to compensate for the deletions in 16S rRNA. The mRNA exit site is highly remodeled due to the presence of cS23 suggesting an alternative mode of translation initiation. bTHXc is positioned at the SSU head and appears to stabilize the intersubunit bridge B1b during thermal fluctuations. The translation factor plastid pY binds to the SSU on the intersubunit side and interacts with the conserved nucleotide bases involved in decoding. Most of the intersubunit bridges are conserved compared to the bacteria, except for a new bridge involving uL2c and bS6c.
History
DepositionMar 3, 2017-
Header (metadata) releaseJun 14, 2017-
Map releaseJun 14, 2017-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5x8p
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_6709.png

Downloads & links

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Map

FileDownload / File: emd_6709.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.05 Å
Density
Contour LevelBy AUTHOR: 0.07 / Movie #1: 0.07
Minimum - Maximum-0.30330205 - 0.47512025
Average (Standard dev.)0.0017467819 (±0.015941085)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 403.19998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.051.051.05
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z403.200403.200403.200
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.3030.4750.002

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Supplemental data

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Sample components

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Entire : Chloroplast 70S ribosome from spinach

EntireName: Chloroplast 70S ribosome from spinach
Components
  • Complex: Chloroplast 70S ribosome from spinach
    • Complex: chloroplast ribosome 50S large subunit
      • Protein or peptide: 50S ribosomal protein L32, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L33, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L34, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L35, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L36, chloroplasticRibosome
      • Protein or peptide: protein cL37
      • Protein or peptide: protein cL38
      • RNA: 5S rRNA5S ribosomal RNA
      • Protein or peptide: 50S ribosomal protein L2, chloroplasticRibosome
      • Protein or peptide: protein L3
      • Protein or peptide: 50S ribosomal protein L4, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L5, chloroplasticRibosome
      • Protein or peptide: protein L6
      • Protein or peptide: protein L9
      • Protein or peptide: 50S ribosomal protein L13, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L14, chloroplasticRibosome
      • Protein or peptide: protein L15
      • Protein or peptide: 50S ribosomal protein L16, chloroplasticRibosome
      • Protein or peptide: protein L17
      • Protein or peptide: protein L18
      • Protein or peptide: 50S ribosomal protein L19, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L20, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L21, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L22, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L23, chloroplasticRibosome
      • Protein or peptide: 50S ribosomal protein L24, chloroplasticRibosome
      • RNA: 4.8S rRNA
      • Protein or peptide: protein L27
      • Protein or peptide: protein L28
      • Protein or peptide: protein L29
      • Protein or peptide: protein bTHXc
      • Protein or peptide: 30S ribosomal protein S1, chloroplasticRibosome
    • Complex: chloroplast ribosome 30S small subunit
      • RNA: 23S rRNA23S ribosomal RNA
      • Protein or peptide: 50S ribosomal protein L31
      • Protein or peptide: 30S ribosomal protein S2, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S3, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S5, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S6 alpha, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S7, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S8, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S9, chloroplasticRibosome
      • Protein or peptide: protein S10
      • Protein or peptide: 30S ribosomal protein S11, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S12, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S13, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S15, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S16, chloroplasticRibosome
      • Protein or peptide: protein S17
      • Protein or peptide: 30S ribosomal protein S18, chloroplasticRibosome
      • Protein or peptide: 30S ribosomal protein S19 alpha, chloroplasticRibosome
      • Protein or peptide: protein S20
      • Protein or peptide: protein S21
      • Protein or peptide: protein plastid pY
      • RNA: 16S rRNA
      • Protein or peptide: protein cS23
      • Protein or peptide: 30S ribosomal protein S4, chloroplasticRibosome
      • Protein or peptide: protein cS22
      • Protein or peptide: 30S ribosomal protein S14, chloroplasticRibosome

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Supramolecule #1: Chloroplast 70S ribosome from spinach

SupramoleculeName: Chloroplast 70S ribosome from spinach / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#58
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 1.7 MDa

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Supramolecule #2: chloroplast ribosome 50S large subunit

SupramoleculeName: chloroplast ribosome 50S large subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#30, #57-#58

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Supramolecule #3: chloroplast ribosome 30S small subunit

SupramoleculeName: chloroplast ribosome 30S small subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #31-#56

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Macromolecule #1: 50S ribosomal protein L32, chloroplastic

MacromoleculeName: 50S ribosomal protein L32, chloroplastic / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 6.519773 KDa
SequenceString:
AVPKKRTSIY KKRIRKNIWK KKGYWAALKA FSLAKSLSTG NSKSFFVRKI SNQTLE

UniProtKB: Large ribosomal subunit protein bL32c

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Macromolecule #2: 50S ribosomal protein L33, chloroplastic

MacromoleculeName: 50S ribosomal protein L33, chloroplastic / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 7.536926 KDa
SequenceString:
AKGKDVRVKV ILECTGCVRK SVNKGSRGVS RYITQKNRHN TPSRLELRKF CPYCYKHTIH GEIKK

UniProtKB: Large ribosomal subunit protein bL33c

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Macromolecule #3: 50S ribosomal protein L34, chloroplastic

MacromoleculeName: 50S ribosomal protein L34, chloroplastic / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 6.789144 KDa
SequenceString:
GKAALCLTKR SRSRKSLART HGFRLRMSTT SGRALLKRRR AKGRKILCTK TNPSSGKRAS P

UniProtKB: Large ribosomal subunit protein bL34c

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Macromolecule #4: 50S ribosomal protein L35, chloroplastic

MacromoleculeName: 50S ribosomal protein L35, chloroplastic / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 8.459163 KDa
SequenceString:
AKGYKMKTHK ASAKRFRVTG KGKIVRRRAG KQHLLAKKNT KRKNRLSKLI QVDRSDYDNV IGALPYLKVN RKV

UniProtKB: Large ribosomal subunit protein bL35c

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Macromolecule #5: 50S ribosomal protein L36, chloroplastic

MacromoleculeName: 50S ribosomal protein L36, chloroplastic / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 4.414462 KDa
SequenceString:
MKIRASVRPI CEKCRLIRRR GRIIVICSNP KHKQRQG

UniProtKB: Large ribosomal subunit protein bL36c

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Macromolecule #6: protein cL37

MacromoleculeName: protein cL37 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 15.694817 KDa
SequenceString:
MALLSPLLSL SSVPPITSIA VSSSSFPIKL QNVSVALLPT LGQRLMTHGP VIAQKRGTVV AMVSAAADET AGEDGDQSKV EEANISVQN LPLESKLQLK LEQKMKMKMA KKIRLRRNRL MRKRKLRKRG AWPPSKMKKL KNV

UniProtKB: Large ribosomal subunit protein cL37 alpha

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Macromolecule #7: protein cL38

MacromoleculeName: protein cL38 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 12.080688 KDa
SequenceString:
MSVSAIFGAR VVTIPSVLRT SSVDGRTVKL QPSTGGSCGG GVITIECSSR PQKKGTAHHM KTRPKKTARW DIKRGPAVYP PLPPLPAEW TIVSSAVDEA DSSSSTTSSS AEIAQSA

UniProtKB: Large ribosomal subunit protein cL38

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Macromolecule #9: 50S ribosomal protein L2, chloroplastic

MacromoleculeName: 50S ribosomal protein L2, chloroplastic / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 29.722424 KDa
SequenceString: AIHLYKTSTS STRNGAVQVK SNPRNNLISG QRRCGKGRNA RGIITARHRG GGHKRLYRKI DFRRNEKDIY GKIVTIEYDP NRNAYICLI HYGDGEKRYI LHPRGAIIGD TIVSGTEVPI KMGNALPLTD MPLGTAIHNI EITLGRGGQL ARAAGAVAKL I AKEGKSAT ...String:
AIHLYKTSTS STRNGAVQVK SNPRNNLISG QRRCGKGRNA RGIITARHRG GGHKRLYRKI DFRRNEKDIY GKIVTIEYDP NRNAYICLI HYGDGEKRYI LHPRGAIIGD TIVSGTEVPI KMGNALPLTD MPLGTAIHNI EITLGRGGQL ARAAGAVAKL I AKEGKSAT LKLPSGEVRL ISKNCSATVG QVGNVGVNQK RLGRAGSKRW LGKRPVVRGV VMNPVDHPHG GGEGRAPIGR KS PTTPWGY PALGRRSRKR NKYSDNFIIR RRSK

UniProtKB: Large ribosomal subunit protein uL2cz/uL2cy

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Macromolecule #10: protein L3

MacromoleculeName: protein L3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 24.117328 KDa
SequenceString: SVDAGIGVMG TKLGMMSFFE EDGTVVPVTV IGFKEGNIVT QVKTESTDGY NAVQVGYERL RDRKLTMPER GHLNKAGVIP MRHLQEFRL VSVDDFTPSQ KLLFEELFKE GDMVDISGTT IGKGFQGGIK RHNFKRGLMT HGSKSHRALG SIGAGTTPGH V YKGKKMPG ...String:
SVDAGIGVMG TKLGMMSFFE EDGTVVPVTV IGFKEGNIVT QVKTESTDGY NAVQVGYERL RDRKLTMPER GHLNKAGVIP MRHLQEFRL VSVDDFTPSQ KLLFEELFKE GDMVDISGTT IGKGFQGGIK RHNFKRGLMT HGSKSHRALG SIGAGTTPGH V YKGKKMPG RMGGTKTKIR KLKIMKIDTD LRVVMIKGAV PGKPGNLLRL APAKIVGKNI PKN

UniProtKB: Large ribosomal subunit protein uL3c

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Macromolecule #11: 50S ribosomal protein L4, chloroplastic

MacromoleculeName: 50S ribosomal protein L4, chloroplastic / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 27.202697 KDa
SequenceString: ELIPLPILNF SGEKVGETFL NLKTAPPEKA RAVVHRGLIT HLQNKRRGTA STLTRAEVRG GGRKPYPQKK TGRARRGSQG SPLRPGGGV IFGPKPRDWT IKMNKKERRL ALSTAIASAV GNSFVVEEFA ENFEKPKTKD FIAAMQRWGL DPAEKSLFFL M DLVENVEK ...String:
ELIPLPILNF SGEKVGETFL NLKTAPPEKA RAVVHRGLIT HLQNKRRGTA STLTRAEVRG GGRKPYPQKK TGRARRGSQG SPLRPGGGV IFGPKPRDWT IKMNKKERRL ALSTAIASAV GNSFVVEEFA ENFEKPKTKD FIAAMQRWGL DPAEKSLFFL M DLVENVEK SGRNIRTLKL LTPRSLNLFD VLNAEKLVFT EGTIQYLNQR YGVDTLEDED EEEEEEEEGE EVDDGVEDGT PE PAE

UniProtKB: Large ribosomal subunit protein uL4c

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Macromolecule #12: 50S ribosomal protein L5, chloroplastic

MacromoleculeName: 50S ribosomal protein L5, chloroplastic / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 24.248189 KDa
SequenceString: AAGTAVFVDK AEAETINRLK TNYIEKMVPL LKEEFSYSNI LEVPKVVKIV VNCGIGDASQ NAKGLDAAIN ELALITGQRP VKTKAKTSI AGFKVREGMT LGIAVTLRGN LMYSFLDRLI NLALPRTRDF QGVNPNSFDG HGNYSVGFRE QSVFPEIKPE I VGKARGMD ...String:
AAGTAVFVDK AEAETINRLK TNYIEKMVPL LKEEFSYSNI LEVPKVVKIV VNCGIGDASQ NAKGLDAAIN ELALITGQRP VKTKAKTSI AGFKVREGMT LGIAVTLRGN LMYSFLDRLI NLALPRTRDF QGVNPNSFDG HGNYSVGFRE QSVFPEIKPE I VGKARGMD VCITTTAKTD KEAYKLLSLM GMPFREGSGP STLVRKKKLK SHHFDAKKGR RY

UniProtKB: Large ribosomal subunit protein uL5c

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Macromolecule #13: protein L6

MacromoleculeName: protein L6 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 20.263604 KDa
SequenceString:
KESRIGKQPI TVPANVAIAM EGQDLKVKGP LGELSITYPR EVLVEKQESG FLRVRKAVET RRANQMHGLF RTLTDNMVVG VSKGFEKKL QLVGVGYRAT VEGKDLILSL GFSHPVRMAI PDELQVKVEE NTKVTVSGRD KSVVGQFAAT IRSWRPPEPY K GKGVRYVD EVVRRKEGKA GKKK

UniProtKB: Large ribosomal subunit protein uL6c

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Macromolecule #14: protein L9

MacromoleculeName: protein L9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 17.669951 KDa
SequenceString:
QKKVKKIRKI ILKEDIPDLG KKGQLLDVRA GFLRNFLLPL GKAEVVTPLL LKEMKMEDER IEAEKKRVKE EAQQLARMFE TVGAFKVKR KGGKGKQIFG SVTAQDLVDI IKAQLQRDVD KKVVFLPDIR ETGEYIAELK LHPDVTAQVR VTVFAN

UniProtKB: Large ribosomal subunit protein bL9c

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Macromolecule #15: 50S ribosomal protein L13, chloroplastic

MacromoleculeName: 50S ribosomal protein L13, chloroplastic / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 22.774363 KDa
SequenceString: STKCTEEWRQ LKEAVKKEFA IPHVPLDQRW MFTLEEATGP DIWNTTWYPK SADHVPTDKK WYVVDATDLI LGRMASTIAI HIRGKNLAS YTPSVDMGAF VIVVNADKVA VSGKKRTQKL YRRHSGRPGG LKEETFDQLQ KRIPERIIEH AVRGMLPKGR L GRYLFNHL ...String:
STKCTEEWRQ LKEAVKKEFA IPHVPLDQRW MFTLEEATGP DIWNTTWYPK SADHVPTDKK WYVVDATDLI LGRMASTIAI HIRGKNLAS YTPSVDMGAF VIVVNADKVA VSGKKRTQKL YRRHSGRPGG LKEETFDQLQ KRIPERIIEH AVRGMLPKGR L GRYLFNHL KVYKGAEHPH QAQQPIDLPL RDKRIRVEK

UniProtKB: Large ribosomal subunit protein uL13c

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Macromolecule #16: 50S ribosomal protein L14, chloroplastic

MacromoleculeName: 50S ribosomal protein L14, chloroplastic / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 13.484741 KDa
SequenceString:
MIQPQTHLNV ADNSGARELM CIRIIGASNR RYARIGDVIV AVIKEAIPNT PLERSEVIRA VVVRTCKELK RDNGMIIRYD DNAAVIIDQ EGNPKGTRIF GAIARELRQK FAKIVSLAPE VL

UniProtKB: Large ribosomal subunit protein uL14c

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Macromolecule #17: protein L15

MacromoleculeName: protein L15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 20.680062 KDa
SequenceString: FRLDNLGPQP GSRKKGKRKG RGHAAGQGGS CGFGMRGQKS RSGPGIMRGF EGGQMPLYRR IPKLRGIAGG MRAGLPKYVP INLRDIEVA GFKEGEEVSL ESLKAKGIIN PSGRERRLPL KILGEGELST KLQIKARAFS GSAKEKLEAA GCSVTVLPGR K KYIKESVR ...String:
FRLDNLGPQP GSRKKGKRKG RGHAAGQGGS CGFGMRGQKS RSGPGIMRGF EGGQMPLYRR IPKLRGIAGG MRAGLPKYVP INLRDIEVA GFKEGEEVSL ESLKAKGIIN PSGRERRLPL KILGEGELST KLQIKARAFS GSAKEKLEAA GCSVTVLPGR K KYIKESVR KNLARADEYF AKKRAASASE AESA

UniProtKB: Large ribosomal subunit protein uL15c

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Macromolecule #18: 50S ribosomal protein L16, chloroplastic

MacromoleculeName: 50S ribosomal protein L16, chloroplastic / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 15.328068 KDa
SequenceString:
MLSPKRTRFR KQHRGRMKGI SYRGNRICFG RYALQALEPA WITSRQIEAG RRAMTRNARR GGKIWVRIFP DKPVTVRPAE TRMGSGKGS PEYWVAVVKP GRILYEISGV AENIARRAVA IAASKMPIRT QFIISG

UniProtKB: Large ribosomal subunit protein uL16c

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Macromolecule #19: protein L17

MacromoleculeName: protein L17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 13.466902 KDa
SequenceString:
MKHGRKIHRL SRPADQRRAL LRGLTTQLLK HGRIKTTRAK ASAMRKYVDK MITLAKEGSL HKRRQALGFI YEKQIVHALF AEVPERYGD RNGGYTRIIR TLPRRGDNAP MAYIELV

UniProtKB: Large ribosomal subunit protein bL17c

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Macromolecule #20: protein L18

MacromoleculeName: protein L18 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 13.801939 KDa
SequenceString:
AKAHTRREDR TARHVRIRKK VEGTPERPRL CVFRSNKHLY VQVIDDTKMH TLAAASTMQK AISENIDYSA GPTVEVAQKI GEMIAKSCL EKGITKVAFD RGGYPYHGRV KALADAAREH GLVF

UniProtKB: Large ribosomal subunit protein uL18c

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Macromolecule #21: 50S ribosomal protein L19, chloroplastic

MacromoleculeName: 50S ribosomal protein L19, chloroplastic / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 17.597373 KDa
SequenceString:
SEAEDSSDAP AESVAVVAEE ELPVESEAEA EERPPRQQRV KLGDIMGILN KKAVHAAEEL RPVPGIRTGD IVQIRLEVPE NKRRLSVYK GIVISRQNAG IHTTIRIRRI IAGVGVEIVF PLYSPNIKEI KVVSHRKVRK ARLYYLRDKL PRLSTFK

UniProtKB: Large ribosomal subunit protein bL19c

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Macromolecule #22: 50S ribosomal protein L20, chloroplastic

MacromoleculeName: 50S ribosomal protein L20, chloroplastic / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 15.594491 KDa
SequenceString:
TRVKRGYIAR RRRKKIRFFA SSFRGAHSRL TRTIAQQKIR ALVSAHRDRD RQKRDFRRLW ITRINAAIRE RGVYYNYSKF IHDLYKRQL LLNRKILAQI AILNPNCIYM IYNEIIKKED CKKYLEII

UniProtKB: Large ribosomal subunit protein bL20c

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Macromolecule #23: 50S ribosomal protein L21, chloroplastic

MacromoleculeName: 50S ribosomal protein L21, chloroplastic / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 22.793986 KDa
SequenceString: AKRRRFQEIP EELKAEFEEF QRPPNQKPQL SDVLPDDFQA PEPGTPEYND IINQFLPKKG PPPPREEIFA VVVIGSRQYI VIPGRWIYT QRLKGATVND KIVLNKVLLV GTKASTYIGT PIVTNAAVHA VVEEQLLDDK VIVFKYKKKK NYRRNIGHRQ P ITRIKITG ...String:
AKRRRFQEIP EELKAEFEEF QRPPNQKPQL SDVLPDDFQA PEPGTPEYND IINQFLPKKG PPPPREEIFA VVVIGSRQYI VIPGRWIYT QRLKGATVND KIVLNKVLLV GTKASTYIGT PIVTNAAVHA VVEEQLLDDK VIVFKYKKKK NYRRNIGHRQ P ITRIKITG ITGYEDYPAS TLEAEVEAKE EAEAEAEAEA VPV

UniProtKB: Large ribosomal subunit protein bL21c

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Macromolecule #24: 50S ribosomal protein L22, chloroplastic

MacromoleculeName: 50S ribosomal protein L22, chloroplastic / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 23.292676 KDa
SequenceString: MGFFKKKEKK AEFDVFRLYG LHHPEPGKCD EITTRGYSIS MSVDKARRVI DQIRGRSYAE TLMILELMPY RACYPIFKLI YSAAANASH NKQFNKANLI ISKAEVNKGI TLKKVKPRAR GRSYMIKRPT CHITIVLRDI THFDSYDKFL ESLTPKKLIA L LGLMSTGR ...String:
MGFFKKKEKK AEFDVFRLYG LHHPEPGKCD EITTRGYSIS MSVDKARRVI DQIRGRSYAE TLMILELMPY RACYPIFKLI YSAAANASH NKQFNKANLI ISKAEVNKGI TLKKVKPRAR GRSYMIKRPT CHITIVLRDI THFDSYDKFL ESLTPKKLIA L LGLMSTGR RRELLCGRFR ENHKIKSFLY KIALFKRYEV M

UniProtKB: Large ribosomal subunit protein uL22c

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Macromolecule #25: 50S ribosomal protein L23, chloroplastic

MacromoleculeName: 50S ribosomal protein L23, chloroplastic / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 13.575806 KDa
SequenceString:
ATEAVAPTTE EAATSQPKTS KKAKKLKYPR RILDVYQILQ SPIITEAAIK NIADENSLLF TVDVRADKKM IREAISNFFG VKVRKVNTL IRPDGTKKAY IMLNKEYNAS ELAKKIGIFP GGN

UniProtKB: Large ribosomal subunit protein uL23c

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Macromolecule #26: 50S ribosomal protein L24, chloroplastic

MacromoleculeName: 50S ribosomal protein L24, chloroplastic / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 16.552316 KDa
SequenceString:
RIKRWERKDC KPNSLPKLHK RHVKVGDTVK VISGGEKGKI GEISKIHKHN STVIIKDLNF KTKHVKSKEE GEQGQIIKIE AAIHSSNVM LILKEQEVAD RVGHKILEDV RKVRYLIKTG EIVDTPDRWK EIQNKKESET AVAVAA

UniProtKB: Large ribosomal subunit protein uL24c

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Macromolecule #28: protein L27

MacromoleculeName: protein L27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 15.326538 KDa
SequenceString:
AHKKGAGSTK NGRDSKGQRL GVKIYGDQVA KPGAIIIRQR GTKFHPGKNV GIGKDHTIFA LIDGLVKFEK YGPDKKKVSV YPREIQPEN PNSYRARKRE NFRLQREKKK ARREGYSFQP QLILASAATD NADESAVC

UniProtKB: Large ribosomal subunit protein bL27c

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Macromolecule #29: protein L28

MacromoleculeName: protein L28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 9.016668 KDa
SequenceString:
RRICPFTGKK SNKANRVSHS NHKTKRLQFV NLQYKRVWWE AGKRFVKLRL STKALKTIEK NGLDAVAKKA GIDLRKE

UniProtKB: Large ribosomal subunit protein bL28c

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Macromolecule #30: protein L29

MacromoleculeName: protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 12.903116 KDa
SequenceString:
KKEDELKELR TKTNEQLNEE ILQLKGELFM LRLQRSAREN FKPSDFGRMR KRVARMLTVK REREIEQGVG KRLSRKLDKA WKRSIVVRP PPSLKKLQEK ATAEAEAEKA

UniProtKB: Large ribosomal subunit protein uL29c

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Macromolecule #32: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 10.801521 KDa
SequenceString:
RKSDIHPEFR EDAKVYCNGE LVMTTGGTQK DYTVEVWSGN HPFYLGNRSA LLLDADQVEK FRKKYGELTQ IMEIPVLKGE IILPPKKKS KAKKK

UniProtKB: Large ribosomal subunit protein bL31c

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Macromolecule #33: 30S ribosomal protein S2, chloroplastic

MacromoleculeName: 30S ribosomal protein S2, chloroplastic / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 26.736141 KDa
SequenceString: MTRRYWNINL EEMMEAGVHF GHGTRKWNPR MSPYISAKCK GIHIINLTRT ARFLSEACDL VFDASSRGKQ FLIVGTKNKA ADSVARAAI RARCHYVNKK WLGGMLTNWS TTETRLHKFR DLRMEQTAGR LARLPKRDAA VVKRQLSHLQ TYLGGIKYMT G LPDIVIIV ...String:
MTRRYWNINL EEMMEAGVHF GHGTRKWNPR MSPYISAKCK GIHIINLTRT ARFLSEACDL VFDASSRGKQ FLIVGTKNKA ADSVARAAI RARCHYVNKK WLGGMLTNWS TTETRLHKFR DLRMEQTAGR LARLPKRDAA VVKRQLSHLQ TYLGGIKYMT G LPDIVIIV DQQEEYTALR ECITLGIPTI CLIDTNCNPD LADISIPAND DAIASIRLIL TKLVFAICEG RSSYIRNP

UniProtKB: Small ribosomal subunit protein uS2c

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Macromolecule #34: 30S ribosomal protein S3, chloroplastic

MacromoleculeName: 30S ribosomal protein S3, chloroplastic / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 24.965156 KDa
SequenceString: MGQKINPLGF RLGTTQSHYS LWFSQPKNYA EGLQEDQKIR DCIKNYVQKN TKTSSGVEGI ARIEIQKRID LIQVIIHMGF PKLLIENRP QGVEDLKINV QKELNCVNRK LNIAITRIAK PYGDPNILAE FIAGQLKSRV SFRKAMKKAI ELTEQADTKG I QIQIAGRI ...String:
MGQKINPLGF RLGTTQSHYS LWFSQPKNYA EGLQEDQKIR DCIKNYVQKN TKTSSGVEGI ARIEIQKRID LIQVIIHMGF PKLLIENRP QGVEDLKINV QKELNCVNRK LNIAITRIAK PYGDPNILAE FIAGQLKSRV SFRKAMKKAI ELTEQADTKG I QIQIAGRI DGKEIARIEW IREGRVPLQT IRAKIDYCAY TVRTIYGVLG IKIWIFMGEE

UniProtKB: Small ribosomal subunit protein uS3c

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Macromolecule #35: 30S ribosomal protein S5, chloroplastic

MacromoleculeName: 30S ribosomal protein S5, chloroplastic / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 27.69658 KDa
SequenceString: CIKKDDIDIT FFEQDNPDEE ITFDPPEKPE GYIPPRAVDE PPFESEEEIA LAYEELYGAA YSGESLLGND VYAMDSKIKK ATGFGSKSK KEKIRDGFEE NVVQVRRVTK VVKGGKHMRF RAIVVVGDKK GQVGVGVGKA KEVVSAVQKA AVDARRNIIT V PMTKYLTF ...String:
CIKKDDIDIT FFEQDNPDEE ITFDPPEKPE GYIPPRAVDE PPFESEEEIA LAYEELYGAA YSGESLLGND VYAMDSKIKK ATGFGSKSK KEKIRDGFEE NVVQVRRVTK VVKGGKHMRF RAIVVVGDKK GQVGVGVGKA KEVVSAVQKA AVDARRNIIT V PMTKYLTF PHRNEADYGA ARVMLRPAAP GTGVIAGGAV RTVLEMAGVE NALGKQLGSN NALNNARATI VAVQTMRQFS DV ARDRGIP MEELWK

UniProtKB: Small ribosomal subunit protein uS5c

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Macromolecule #36: 30S ribosomal protein S6 alpha, chloroplastic

MacromoleculeName: 30S ribosomal protein S6 alpha, chloroplastic / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 16.341423 KDa
SequenceString:
DFSGSFFEGG FGGLDEDPPS TPPAGLAVEE KPEPQCPPGL RQYETMAVLR PDMTEDERLT LTQKYEELLV AGGAMYVEVF NRGVIPLAY SIKRKNKAGE TNNYLDGIYL LFTYFTKPES ISPLEAALVT DDDVIRSSSF KIRKRKY

UniProtKB: Small ribosomal subunit protein bS6c alpha

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Macromolecule #37: 30S ribosomal protein S7, chloroplastic

MacromoleculeName: 30S ribosomal protein S7, chloroplastic / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 17.378309 KDa
SequenceString:
MSRRGTVEEK TAKSDPIYRN RLVNMLVNRI LKHGKKSLAY QILYRAVKKI QQKTETNPLS VLRQAIRGVT PDIAVKARRV GGSTHQVPI EIGSTQGKAL AIRWLLGAAR KRPGRNMAFK LSSELVDAAK GSGDAVRKKE ETHRMAEANR AFAHFR

UniProtKB: Small ribosomal subunit protein uS7cz/uS7cy

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Macromolecule #38: 30S ribosomal protein S8, chloroplastic

MacromoleculeName: 30S ribosomal protein S8, chloroplastic / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 15.527256 KDa
SequenceString:
MGKDTIADII TCIRNADMNR KGTVRIVSTN ITENIVKILL REGFIENARK HQERNKYFLV LTLRHRRNKK GPYLNTFHLK RVSRPGLRI YSNYQRIPRI LGGMGIAILS TSRGIMTDRE ARLEGIGGEI LCYIW

UniProtKB: Small ribosomal subunit protein uS8c

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Macromolecule #39: 30S ribosomal protein S9, chloroplastic

MacromoleculeName: 30S ribosomal protein S9, chloroplastic / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 17.092791 KDa
SequenceString:
TSATAPVEVA ETADLEKFVK SRLPGGFAAQ TVIGTGRRKC AIARVVLQEG TGKFIINYRD AKEYLQGNPL WLQYVKTPLA TLGYETNYD VFVKAHGGGL SGQAQAISLG VARALLKVSA SHRAPLKQEG LLTRDSRIVE RKKPGLKKAR KAPQFSKR

UniProtKB: Small ribosomal subunit protein uS9c

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Macromolecule #40: protein S10

MacromoleculeName: protein S10 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 13.882167 KDa
SequenceString:
SFEDGSETSK ISIAADSDQM APKQKIRIKL RSYWVPLIED SCKQIMDAAR TTNAKIMGPV PLPTKKRIYC VLKSPHVHKD ARFHFEIRT HQRLIDILYP TAQTIDSLMQ LDLPAGVDVE VKL

UniProtKB: Small ribosomal subunit protein uS10c

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Macromolecule #41: 30S ribosomal protein S11, chloroplastic

MacromoleculeName: 30S ribosomal protein S11, chloroplastic / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 14.927551 KDa
SequenceString:
MAKPIPKIGS RRNGRISSRK SARKIPKGVI HVQASFNNTI VTVTDVRGRV VSWASAGTCG FRGTKRGTPF AAQTAAGNAI RTVVEQGMQ RAEVMIKGPG LGRDAALRAI RRSGILLSFV RDVTPMPHNG CRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11c

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Macromolecule #42: 30S ribosomal protein S12, chloroplastic

MacromoleculeName: 30S ribosomal protein S12, chloroplastic / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 13.794261 KDa
SequenceString:
MPTIKQLIRN TRQPIRNVTK SPALRGCPQR RGTCTRVYTI TPKKPNSALR KVARVRLTSG FEITAYIPGI GHNLQEHSVV LVRGGRVKD LPGVRYHIVR GTLDAVGVKD RQQGRSKYGV KKPK

UniProtKB: Small ribosomal subunit protein uS12cz/uS12cy

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Macromolecule #43: 30S ribosomal protein S13, chloroplastic

MacromoleculeName: 30S ribosomal protein S13, chloroplastic / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 14.418822 KDa
SequenceString:
ARGRSSSVSF PAPKKGGIGH GGLQIECIRI GGVEIPNHKR VEYSLQYIHG IGRSRSRQIL LDLNFDNKVT KDLSEEEVII LRKEKRFNR VAIERLKEIR CYRGIRHKLG LPVRGQRTKN NCRTLKG

UniProtKB: Small ribosomal subunit protein uS13c

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Macromolecule #44: 30S ribosomal protein S15, chloroplastic

MacromoleculeName: 30S ribosomal protein S15, chloroplastic / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 10.778763 KDa
SequenceString:
MKKNSFISVI SDEKKEENKG SVEFQVFCFT NKIRRLTLHL ELHKKDYSSQ RGLRKTLGKR QRLLAYLLKI NGVRYKELIS KLNIRELKT R

UniProtKB: Small ribosomal subunit protein uS15c

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Macromolecule #45: 30S ribosomal protein S16, chloroplastic

MacromoleculeName: 30S ribosomal protein S16, chloroplastic / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 10.454237 KDa
SequenceString:
MVKLRLKRCG RKQRAVYRIV AIDVRSRREG RDLQKVGFYD PIKSQTYLNV PAILDFLEKG AQPTETVYDI LKRAEVFKEF RLNQTKFN

UniProtKB: Small ribosomal subunit protein bS16c

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Macromolecule #46: protein S17

MacromoleculeName: protein S17 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 12.195099 KDa
SequenceString:
MRSMQGKVIC ATNDKTVNVE VVRLAPHPKY KRRVRKKKKY QAHDPDNQFK VGDWVQLDKC RPISKTKTFL AVAPEGRQSS ATRPKPIQA ASDELGIPLE SQVEGDKTV

UniProtKB: Small ribosomal subunit protein uS17c

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Macromolecule #47: 30S ribosomal protein S18, chloroplastic

MacromoleculeName: 30S ribosomal protein S18, chloroplastic / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 12.079137 KDa
SequenceString:
MDKSKRPFIK SKRSFRRRLP PIQSGDRIDY RNMSLISRFI SEQGKILSRR VNRLTLKQQR LITSAIKQAR ILSLLPFLNN EKQFERTES TTRTANFRTK NK

UniProtKB: Small ribosomal subunit protein bS18c

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Macromolecule #48: 30S ribosomal protein S19 alpha, chloroplastic

MacromoleculeName: 30S ribosomal protein S19 alpha, chloroplastic / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 10.6325 KDa
SequenceString:
MTRSLKKNPF VANHLLRKIE KLNKKAEKEI IVTWSRASTI IPTMIGHTIA IHNGREHLPI YITDRMVGHK LGEFAPTLNF RGHAKNDNK SRR

UniProtKB: Small ribosomal subunit protein uS19c

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Macromolecule #49: protein S20

MacromoleculeName: protein S20 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 12.17809 KDa
SequenceString:
ADSAAKRTRQ AETRRLRNKA RKSEVKTRMR KVFEALDALK KKSGASTEEL VPIDNLIAEA YSAIDKAVVK GTLHRNTAAR RKSRLARNK KVVEIHHGWY TPSLAPTNV

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Macromolecule #50: protein S21

MacromoleculeName: protein S21 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 16.096171 KDa
SequenceString:
ISSKKHENAA IAKKEEYPGD LMAVVCPSLA FSNTLYFRSA YNVQVLVDDN ENEERLLNRF RREVMRAGVI QECKRRRYFE NKQEEKKRK HREAAKRNSR RRRGPFRGPF PGKEEATKVD KKEDDGDNWD MPEGGAPF

UniProtKB: Small ribosomal subunit protein bS21c

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Macromolecule #51: protein plastid pY

MacromoleculeName: protein plastid pY / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 26.85133 KDa
SequenceString: SWDGPLSSVK LILQGRNLEV SDNVRSHVED KVGKSVAKHS HLVREVDVRL SARGGDLSKG PKLRRCEVTL FTKRHGVIRA EEDAESLYS SIDLVSSIIQ RKLRKIKDKV SDHGRHMKGF NRSKVRDPEP VRITREEVLE EVESAPAPVS VEDDDFIEEV V RTKYFDMP ...String:
SWDGPLSSVK LILQGRNLEV SDNVRSHVED KVGKSVAKHS HLVREVDVRL SARGGDLSKG PKLRRCEVTL FTKRHGVIRA EEDAESLYS SIDLVSSIIQ RKLRKIKDKV SDHGRHMKGF NRSKVRDPEP VRITREEVLE EVESAPAPVS VEDDDFIEEV V RTKYFDMP PLTITEAVEQ LENVDHDFYA FRNEETGDIN ILYKRKEGGY GLIIPKDGKT EKLESLPVQT DKQPSFAE

UniProtKB: Ribosome-binding factor PSRP1, chloroplastic

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Macromolecule #53: protein cS23

MacromoleculeName: protein cS23 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 13.808066 KDa
SequenceString:
VAPETISDVA IMGNEVDIDD DLLVNKEKLK VLVKPMDKPR LVLKFIWMEK NIGLALDQTI PGHGTVPLSP YYFWPRKDAW EELKVLLEN KPWISQKQMI ILLNQATDII NLWQQSGGNL AS

UniProtKB: Small ribosomal subunit protein cS23

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Macromolecule #54: 30S ribosomal protein S4, chloroplastic

MacromoleculeName: 30S ribosomal protein S4, chloroplastic / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 23.454531 KDa
SequenceString: MSRYRGPRFK KIRRLGALPG LTNKRPRAGS DLRNQSRSGK RSQYRIRLEE KQKLRFHYGI TERQLLKYVR IARKAKGSTG QVLLQLLEM RLDNILFRLG MAPTIPGARQ LVNHRHILVN GRIVDIPSYR CKPQDTIMAR DEQKSIALIQ NSLDLSPREE L PKHLTLNP ...String:
MSRYRGPRFK KIRRLGALPG LTNKRPRAGS DLRNQSRSGK RSQYRIRLEE KQKLRFHYGI TERQLLKYVR IARKAKGSTG QVLLQLLEM RLDNILFRLG MAPTIPGARQ LVNHRHILVN GRIVDIPSYR CKPQDTIMAR DEQKSIALIQ NSLDLSPREE L PKHLTLNP FPYKGLVNQI IDSKWVGLKI NELLVVEYYS RQT

UniProtKB: Small ribosomal subunit protein uS4c

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Macromolecule #55: protein cS22

MacromoleculeName: protein cS22 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 21.691145 KDa
SequenceString: VVTEETSSSS TASSSSDGEG ARRLYVGNIP RNLNNDELRT IVEEHGAIEI AEVMYDKYSG RSRRFGFVTM KTVEDANAVI EKLNDTEIG GRKIKVNITE KPLEGMDIAT TQAEDSQFVE SPYKVYIGNL AKTVTNELLK DFFSEKGKVL GAKVQRTPGT S KSNGFGFV ...String:
VVTEETSSSS TASSSSDGEG ARRLYVGNIP RNLNNDELRT IVEEHGAIEI AEVMYDKYSG RSRRFGFVTM KTVEDANAVI EKLNDTEIG GRKIKVNITE KPLEGMDIAT TQAEDSQFVE SPYKVYIGNL AKTVTNELLK DFFSEKGKVL GAKVQRTPGT S KSNGFGFV SFSSEEEVEA AIQALNNSVL EGQKIRVNKA

UniProtKB: Small ribosomal subunit protein cS22

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Macromolecule #56: 30S ribosomal protein S14, chloroplastic

MacromoleculeName: 30S ribosomal protein S14, chloroplastic / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 11.809832 KDa
SequenceString:
MARKSLIQRE KKRRNLEQKY HLIRRSSKQE IRKVTSLSDK WEIHGKLQSP PRNSAPARLH RRCFLTGRPR ANIRDFGLSG HILREMVHT CLLPGATRSS W

UniProtKB: Small ribosomal subunit protein uS14c

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Macromolecule #57: protein bTHXc

MacromoleculeName: protein bTHXc / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 5.188887 KDa
SequenceString:
GRGDRKTAKG KRFNHSFGNA RPKNKNKGRG PPKAPIFPKG DPSQKED

UniProtKB: Small ribosomal subunit protein bTHXc

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Macromolecule #58: 30S ribosomal protein S1, chloroplastic

MacromoleculeName: 30S ribosomal protein S1, chloroplastic / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 40.472961 KDa
SequenceString: AVAVSNAQTR ERQKLKQLFE DAYERCRNAP MEGVSFTIDD FHTALDKYDF NSEMGSRVKG TVFCTDANGA LVDITAKSSA YLPLAEACI YRIKNVEEAG IIPGVREEFV IIGENEADDS LILSLRQIQY ELAWERCRQL QAEDVVVKGK IVGANKGGVV A LVEGLRGF ...String:
AVAVSNAQTR ERQKLKQLFE DAYERCRNAP MEGVSFTIDD FHTALDKYDF NSEMGSRVKG TVFCTDANGA LVDITAKSSA YLPLAEACI YRIKNVEEAG IIPGVREEFV IIGENEADDS LILSLRQIQY ELAWERCRQL QAEDVVVKGK IVGANKGGVV A LVEGLRGF VPFSQISSKS SAEELLEKEI PLKFVEVDEE QSRLVMSNRK AMADSQAQLG IGSVVTGTVQ SLKPYGAFID IG GINGLLH VSQISHDRVS DIATVLQPGD TLKVMILSHD RERGRVSLST KKLEPTPGDM IRNPKLVFEK AEEMAQTFRQ RIA QAEAMA RADMLRFQPE SGLTLSSDGI LGPLTSDLPA EGLDLSVVPP AVES

UniProtKB: Small ribosomal subunit protein bS1c

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Macromolecule #8: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 8 / Number of copies: 1
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 39.014184 KDa
SequenceString:
UAUUCUGGUG UCCUAGGCGU AGAGGAACCA CACCAAUCCA UCCCGAACUU GGUGGUUAAA CUCUACUGCG GUGACGAUAC UGUAGGGGA GGUCCUGCGG AAAAAUAGCU CGACGCCAGG AU

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Macromolecule #27: 4.8S rRNA

MacromoleculeName: 4.8S rRNA / type: rna / ID: 27 / Number of copies: 1
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 34.334488 KDa
SequenceString:
AGAGAAGGUC ACGGCGAGAC GAGCCGUUUA UCAUUACGAU AGGUGUCAAG UGGAAGUGCA GUGAUGUAUG CAGCUGAGGC AUCCUAACA GACCCACAGA CUUGAAC

GENBANK: GENBANK: X04977.1

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Macromolecule #31: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 911.34425 KDa
SequenceString: UUCAAACGAG GAAAGGCUUA CGGUGGAUAC CUAGGCACCC AGAGACGAGG AAGGGCGUAU UAAUCGACGA AAUGCUUCGG GGAGUUGAA AAUAAGCAGA GAUCCGGAGA UUCCCGAAUA GGUCAACCUU UCGAACUUCU GCUGAAUCCA UGGGCAGGCA A GAGACAAC ...String:
UUCAAACGAG GAAAGGCUUA CGGUGGAUAC CUAGGCACCC AGAGACGAGG AAGGGCGUAU UAAUCGACGA AAUGCUUCGG GGAGUUGAA AAUAAGCAGA GAUCCGGAGA UUCCCGAAUA GGUCAACCUU UCGAACUUCU GCUGAAUCCA UGGGCAGGCA A GAGACAAC CUGGCGAACU GAAACAUCUU AGUAGCCAGA GGAAAAGAAA GCAAAAGCGA UUCCCGUAGU AGCGGCGAGC GA AAUGGGA GCAGCCUAAA CCGUGAAAAC GGGGUUGUGG GAGAGCAAUA CAAGCGUCGU GCUGCUAGGC GAAUCAGUGG AGU GCGGAA CCCUAGAUGG UGAAAGUCCA GUAGCCGAAA GCAUCACUAG CUUAUGCUCU GACCCGAGUA GCAUGGGGCA CGUG GAAUC CCGUGUGAAU CAGCAAGGAC CACCUUGCAA GGCUAAAUAC UCCUGGGUGA CCGAUAGCGA AGUAGUACCG UGAGG GAAG GGUGAAAAGA ACCCCCAUCG GGGAGUGAAA UAGAACAUGA AACCGUAAGC UCUCAAGCAG UGGGAGGGGG ACCAGA CCC UGACCGCGUG CCUGUUGAAG AAUGAGCCGG CGACUCAUAG GCAGUGGCUU GGUUAAGGGA ACCCACCGGA GCCGUAG CG AAAGCGAGUC UUCAUAGGGC AAUUGUCACU GCUUAUGGAC CCGAACCUGG GUGAUCUAUC CAUGACCAGG AUGAAGCU U GGGUGAAACU AAGUGGAGGU CCGAACCGAC UGAUGUUGAA GAAUCAGCGG AUGAGUUGUG GUUAGGGGUG AAAUGCCAC UCGAACCCAG AGCUAGCUGG UUCUCCCCGA AAUGCGUUGA GGCGCAGCAG UUGACUGGAC AUCUAGGGGU AAAGCACUGU UUCGGUGCG GGCCGCGAGA GCGGUACCAA AUCGAGGCAA ACUCUGAAUA CUAGAUAUGA CCUCCAAAUA ACAGGGGUCA A GGUCGGCC AGUGAGACGA UGGGGGAUAA GCUUCAUCGU CGAGAGGGAA ACAGCCCGGA UCACCAGCUA AGGCCCCUAA AU GACCGCU CAGUGAUAAA GGAGGUAGGG GUGCAGAGAC AGCCAGGAGG UUUGCCUAGA AGCAGCCACC CUUGAAAGAG UGC GUAAUA GCUCACUGAU CGAGCGCUCU UGCGCCGAAG AUGAACGGGG CUAAGCGGUC UGCCGAAGCU GUGGGAUGUA AAAA AACAU CGGUAGGGGA GCGUUCCGUG UUAGGGAGAA ACGCGUGCGU GAGCCGCGUU GGACGAAGCG GAAGCGAGAA UGUCG GCUU GAGUAACGCA AACAUUGGUG AGAAUCCAAU GCCCCGAAAA CCUAAGGGUU CCUCCGCAAG GUUCGUCCAC GGAGGG UGA GUCAGGGCCU AAGAUCAGGC CGAAAGGCGU AGUCGAUGGA CAACAGGUGA AUAUUCCUGU ACUACCCCUU GUUGGUC CC GAGGGACGGA GGAGGCUAGG UUAGCCGAAA GAUGGUUAUC GGUUCAAGGA CGCAAGGUGA CCCUGUUUUU CAGGGUAA G AAGGGGUAGA GAAAAUGCCU CGAGCCAAUG UUCGAGUACC AGGCGCUACG GCGCUGAAGU AACCGAUGCC AUACUCCCA GGAAAAGCUC GAACGACCUU CAACAAAAGG GUACCUGUAC CCGAAACCGA CACAGGUAGG UAGGUAGAGA AUACCUAGGG GCGCGAGAC AACUCUCUCU AAGGAACUCG GCAAAAUAGC CCCGUAACUU CGGGAGAAGG GGUGCCCCCU CACAAAGGGG G UCGAAGUG ACCAGGCCCG GGCGACUGUU UACCAAAAAC ACAGGUCUCC GCAAAGUCGU AAGACCAUGU AUGGGGGCUG AC GCCUGCC CAGUGCCGGA AGGUCAAGGA AGUUGGUGAC CUGAUGACAG GGGAGCCGGC GACCGAAGCC CCGGUGAACG GCG GCCGUA ACUAUAACGG UCCUAAGGUA GCGAAAUUCC UUGUCGGGUA AGUUCCGACC CGCACGAAAG GCGUAACGAU CUGG GCACU GUCUCGGAGA GAGGCUCGGU GAAAUAGACA UGUCUGUGAA GAUGCGGACU ACCUGCACCU GGACAGAAAG ACCCU AUGA AGCUUUACUG UUCCCUGGGA UUGGCUUUGG GCUUUUCCUG CGCAGCUUAG GUGGAAGGCG AAGAAGGCCC CCUUCC GGG GGGGCCCGAG CCAUCAGUGA GAUACCACUC UGGAAGAGCU AGAAUUCUAA CCUUGUGUCA GGACCUACGG GCCAAGG GA CAUUCUCAGG UAGACAGUUU CUAUGGGGCG UAGGCCUCCC AAAAGGUAAC GGAGGCGUGC AAAGGUUUCC UCGGGCCG G ACGGAGAUUG GCCCUCGAGU GCAAAGGCAG AAGGGAGCUU GACUGCAAGA CCCACCCGUC GAGCAGGGAC GAAAGUCGG CCUUAGUGAU CCGACGGUGC CGAGUGGAAG GGCCGUCGCU CAACGGAUAA AAGUUACUCU AGGGAUAACA GGCUGAUCUU CCCCAAGAG UUCACAUCGA CGGGAAGGUU UGGCACCUCG AUGUCGGCUC UUCGCCACCU GGGGCUGUAG UAUGUUCCAA G GGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGUGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUCC AUAUCCGGUG UG GGCGUUA GAGCAUUGAG AGGACCUUUC CCUAGUACGA GAGGACCGGG AAGGACGCAC CUCUGGUGUA CCAGUUAUCG UGC CCACGG UAAACGCUGG GUAGCCAAGU GCGGAGCGGA UAACUGCUGA AAGCAUCUAA GUAGUAAGCC CACCCCAAGA UGAG UGCUC UCCUA

GENBANK: GENBANK: AJ400848.1

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Macromolecule #52: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 52 / Number of copies: 1
Source (natural)Organism: Spinacia oleracea (spinach)
Molecular weightTheoretical: 483.4665 KDa
SequenceString: UCUCAUGGAG AGUUCGAUCC UGGCUCAGGA UGAACGCUGG CGGCAUGCUU AACACAUGCA AGUCGGACGG GAAGUGGUGU UUCCAGUGG CGGACGGGUG AGUAACGCGU AAGAACCUGC CCUUGGGAGG GGAACAACAG CUGGAAACGG CUGCUAAUAC C CCGUAGGC ...String:
UCUCAUGGAG AGUUCGAUCC UGGCUCAGGA UGAACGCUGG CGGCAUGCUU AACACAUGCA AGUCGGACGG GAAGUGGUGU UUCCAGUGG CGGACGGGUG AGUAACGCGU AAGAACCUGC CCUUGGGAGG GGAACAACAG CUGGAAACGG CUGCUAAUAC C CCGUAGGC UGAGAAGCAA AAGGAGGAAU CCGCCCGAGG AGGGGCUCGC GUCUGAUUAG CUAGUUGGUG AGGUAAUAGC UU ACCAAGG CGAUGAUCAG UAGCUGGUCC GAGAGGAUGA UCAGCCACAC UGGGACUGAG ACACGGCCCA GACUCCUACG GGA GGCAGC AGUGGGGAAU UUUCCGCAAU GGGCGAAAGC CUGACGGAGC AAUGCCGCGU GGAGGCAGAA GGCCCACGGG UCGU GAACU UCUUUUCCCG GAGAAGAAGC AAUGACGGUA UCCGGGGAAU AAGCAUCGGC UAACUCUGUG CCAGCAGCCG CGGUA AGAC AGAGGAUGCA AGCGUUAUCC GGAAUGAUUG GGCGUAAAGC GUCUGUAGGU GGCUUUUUAA GUCCGCCGUC AAAUCC CAG GGCUCAACCC UGGACAGGCG GUGGAAACUA CCAAGCUGGA GUACGGUAGG GGCAGAGGGA AUUUCCGGUG GAGCGGU GA AAUGCGUAGA GAUCGGAAAG AACACCAACG GCGAAAGCAC UCUGCUGGGC CGACACUGAC ACUGAGAGAC GAAAGCUA G GGGAGCGAAU GGGAUUAGAU ACCCCAGUAG UCCUAGCCGU AAACGAUGGA UACUAGGCGC UGUGCGUAUC GACCCGUGC AGUGUUGUAG CUAACGCGUU AAGUAUCCCG CCUGGGGAGU ACGUUCGCAA GAAUGAAACU CAAAGGAAUU GACGGGGGCC CGCACAAGC GGUGGAGCAU GUGGUUUAAU UCGAUGCAAA GCGAAGAACC UUACCAGGGC UUGACAUGCC GCGAAUCCUC U UGAAAGAG AGGGGUGCCU UCGGGAACGC GGACACAGGU GGUGCAUGGC UGUCGUCAGC UCGUGCCGUA AGGUGUUGGG UU AAGUCCC GCAACGAGCG CAACCCUCGU GUUUAGUUGC CAACGUUGAG UUUGGAACCC UGAACAGACU GCCGGUGAUA AGC CGGAGG AAGGUGAGGA UGACGUCAAG UCAUCAUGCC CCUUAUGCCC UGGGCGACAC ACGUGCUACA AUGGCCGGGA CAAA GGGUC GCGAUCCCGC GAGGGUGAGC UAACCCCAAA AACCCGUCCU CAGUUCGGAU UGCAGGCUGC AACUCGCCUG CAUGA AGCC GGAAUCGCUA GUAAUCGCCG GUCAGCCAUA CGGCGGUGAA UUCGUUCCCG GGCCUUGUAC ACACCGCCCG UCACAC UAU GGGAGCUGGC CAUGCCCGAA GUCGUUACCU UAACCGCAAG GAGGGGGAUG CCGAAGGCAG GGCUAGUGAC UGGAGUG AA GUCGUAACAA GGUAGCCGUA CUGGAAGGUG CGGCUGGAUC ACCUCCUUU

GENBANK: GENBANK: AJ400848.1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
Details: 20 mM Tris HCl, pH 7.6, 100 mM KCl, 10 mM MgOAc, 100 mM sucrose, 7 mM 2-mercaptoethanol, 1 unit/ml RNase inhibitor, 0.1% protease inhibitor
GridModel: Quantifoil / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated defocus max: 3.7 µm / Calibrated defocus min: 0.4 µm / Calibrated magnification: 133333 / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.7 µm / Nominal defocus min: 0.4 µm / Nominal magnification: 75000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 1-25 / Number grids imaged: 1 / Number real images: 3161 / Average electron dose: 1.5 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 187946
Startup modelType of model: EMDB MAP
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 81305

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