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- EMDB-3844: 80S from Trypanosoma cruzi -

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Basic information

Entry
Database: EMDB / ID: EMD-3844
Title80S from Trypanosoma cruzi
Map data
Sample
  • Complex: 80S ribosome from Trypanosoma cruziEukaryotic ribosome
    • Protein or peptide: x 34 types
    • RNA: x 1 types
Function / homology
Function and homology information


translation regulator activity / ribosomal small subunit assembly / cytosolic small ribosomal subunit / ribosome binding / small ribosomal subunit / kinase activity / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex ...translation regulator activity / ribosomal small subunit assembly / cytosolic small ribosomal subunit / ribosome binding / small ribosomal subunit / kinase activity / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / phosphorylation / RNA binding / zinc ion binding / metal ion binding
Similarity search - Function
Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 ...Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / S25 ribosomal protein / Ribosomal protein S19A/S15e / Ribosomal protein S30 / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal S17 / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / 40S Ribosomal protein S10 / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S28e / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein S7e / Plectin/S10 domain / Ribosomal protein S8e / Ribosomal protein S4, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / RS4NT (NUC023) domain / Ribosomal protein S27 / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal family S4e / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S8e signature. / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S28e signature. / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein S14/S29 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / RNA-binding domain superfamily / Ribosomal protein S2 signature 2. / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / KH domain / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S10 / Ribosomal protein S3, C-terminal domain / Ribosomal protein S7, conserved site / Ribosomal protein S17, conserved site / K homology domain superfamily, prokaryotic type / Ribosomal protein S19
Similarity search - Domain/homology
40S ribosomal protein SA / 40S ribosomal protein S12 / 40S ribosomal protein S7 / Ribosomal protein S29, putative / 40S ribosomal protein S26 / 40S ribosomal protein S11, putative / Ubiquitin-60S ribosomal protein L40 / 40S ribosomal protein S9, putative / 40S ribosomal protein S4 / Ribosomal protein S20, putative ...40S ribosomal protein SA / 40S ribosomal protein S12 / 40S ribosomal protein S7 / Ribosomal protein S29, putative / 40S ribosomal protein S26 / 40S ribosomal protein S11, putative / Ubiquitin-60S ribosomal protein L40 / 40S ribosomal protein S9, putative / 40S ribosomal protein S4 / Ribosomal protein S20, putative / 40S ribosomal protein S14, putative / 40S ribosomal protein S33, putative / 40S ribosomal protein S30 / 40S ribosomal protein S13, putative / 40S ribosomal protein S16, putative / 40S ribosomal protein S15, putative / 40S ribosomal protein S2, putative / Ribosomal protein S19, putative / 40S ribosomal protein S17, putative / 40S ribosomal protein S3, putative / 40S ribosomal protein S27, putative / 40S ribosomal protein S6 / Guanine nucleotide-binding protein subunit beta-like protein / 40S ribosomal protein S23, putative / 40S ribosomal protein S25 / 40S ribosomal protein S8 / 40S ribosomal protein S24 / RNA-binding protein, putative / 40S ribosomal protein S3a-2 / 40S ribosomal protein S10, putative / 40S ribosomal protein S18, putative / 40S ribosomal protein S15a, putative / 40S ribosomal protein S5, putative / 40S ribosomal protein S21, putative
Similarity search - Component
Biological speciesTrypanosoma cruzi (eukaryote) / Trypanosoma cruzi (strain CL Brener) (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.0 Å
AuthorsBrito Querido J / Mancera-Martinez E / Vicens Q / Bochler A / Chicher J / Simonetti A / Hashem Y
CitationJournal: Structure / Year: 2017
Title: The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein.
Authors: Jailson Brito Querido / Eder Mancera-Martínez / Quentin Vicens / Anthony Bochler / Johana Chicher / Angelita Simonetti / Yaser Hashem /
Abstract: Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti- ...Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti-kinetoplastid therapeutic molecules that can replace the scarce and highly cytotoxic current drugs. The kinetoplastid ribosome represents a potential therapeutic target due to its relative structural divergence when compared with its human counterpart. However, several kinetoplastid-specific ribosomal features remain uncharacterized. Here, we present the near-atomic cryoelectron microscopy structure of a novel bona fide kinetoplastid-specific ribosomal (r-) protein (KSRP) bound to the ribosome. KSRP is an essential protein located at the solvent face of the 40S subunit, where it binds and stabilizes kinetoplastid-specific domains of rRNA, suggesting its role in ribosome integrity. KSRP also interacts with the r-protein eS6 at a region that is only conserved in kinetoplastids. The kinetoplastid-specific ribosomal environment of KSRP provides a promising target for the design of safer anti-kinetoplastidian drugs.
History
DepositionAug 10, 2017-
Header (metadata) releaseNov 15, 2017-
Map releaseNov 15, 2017-
UpdateDec 13, 2017-
Current statusDec 13, 2017Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0549
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.0549
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5opt
  • Surface level: 0.0549
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-5opt
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3844.png

Downloads & links

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Map

FileDownload / File: emd_3844.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.56 Å
Density
Contour LevelBy AUTHOR: 0.0549 / Movie #1: 0.0549
Minimum - Maximum-0.13201863 - 0.25684065
Average (Standard dev.)-0.00023250228 (±0.016643882)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 499.19998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.561.561.56
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z499.200499.200499.200
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.1320.257-0.000

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Supplemental data

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Sample components

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Entire : 80S ribosome from Trypanosoma cruzi

EntireName: 80S ribosome from Trypanosoma cruziEukaryotic ribosome
Components
  • Complex: 80S ribosome from Trypanosoma cruziEukaryotic ribosome
    • Protein or peptide: Activated protein kinase C receptor, putative
    • Protein or peptide: Ribosomal protein S29, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S16, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S15, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S18, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: 40S ribosomal protein S23, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S5, putativeRibosome
    • Protein or peptide: Ribosomal protein S7, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S13, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S27, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S33, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S14, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S3a-2Ribosome
    • Protein or peptide: 40S ribosomal protein S11, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S9, putativeRibosome
    • Protein or peptide: 40S ribosomal protein SA
    • Protein or peptide: 40S ribosomal protein S2, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S15a, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S21, putativeRibosome
    • Protein or peptide: Ribosomal protein S25, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: Ubiquitin/ribosomal protein S27a, putative
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S17, putativeRibosome
    • Protein or peptide: Ribosomal protein S20, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S3, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S10, putativeRibosome
    • Protein or peptide: Ribosomal protein S19, putativeRibosome
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: RNA-binding protein, putative
    • RNA: 18S rRNA18S ribosomal RNA

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Supramolecule #1: 80S ribosome from Trypanosoma cruzi

SupramoleculeName: 80S ribosome from Trypanosoma cruzi / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Trypanosoma cruzi (eukaryote)

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Macromolecule #1: Activated protein kinase C receptor, putative

MacromoleculeName: Activated protein kinase C receptor, putative / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 35.070312 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MAVVYEGQLK GHRGWVTALA CPQITETYIK AVSTSRDNTL IAWGSNMDRN SEECEYGFPE RRLEGHSAFV SDVALSNNGD FAVSASWDH SLRLWNLQTG VCQHKFLGHT KDVLSVTFSP DNRQIVSGGR DNALRVWNVK GECLHTLGRG AHTDWVSCVR F SPSLETPL ...String:
MAVVYEGQLK GHRGWVTALA CPQITETYIK AVSTSRDNTL IAWGSNMDRN SEECEYGFPE RRLEGHSAFV SDVALSNNGD FAVSASWDH SLRLWNLQTG VCQHKFLGHT KDVLSVTFSP DNRQIVSGGR DNALRVWNVK GECLHTLGRG AHTDWVSCVR F SPSLETPL IVSGGWDNLV KVWDIASGRL LTDLKGHTNY ITSVTVSPDG SLCASSDKDG VARLWDLTKG EALSEMAAGA PI NQICFSP NRYWMCAATE KGIRIFDLEN KDVIVELAPE AQQKSKKTPE CMSIAWSADG NTLYSGYTDN VIRVWSVSEH A

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Macromolecule #2: Ribosomal protein S29, putative

MacromoleculeName: Ribosomal protein S29, putative / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 6.651826 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MGHLEQWRSR QKIGMGKGSR HCVICSNQKA VIRKYELNVC RQCFRENAAN IGFSKLR

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Macromolecule #3: 40S ribosomal protein S16, putative

MacromoleculeName: 40S ribosomal protein S16, putative / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 16.841643 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MTAEKSHTHK QVQTFGKKKT AIAVATVTKA AQCNIRVNGV PVSQILPETL RAKIMEAVNV VGSRHFARLR IDVTVRGAGQ VAQAYATRQ AIAKGLVAYY QKYKNEVEKA ALKDKYLAYD KYLLIADPRR CEPKKWGRHS ARTRFTKSYR

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Macromolecule #4: 40S ribosomal protein S15, putative

MacromoleculeName: 40S ribosomal protein S15, putative / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 17.554453 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MTSNITQERY EQLKKERTFH KFTYRGLEID PLLALSEEEF KTLVHARARR HMNRHANRRA PVLLKRLREA KKDVKAGEKP RAVKTHLRD VVITPEMVGS VVGIYNGRQF NAVEIKGEMI GHYLGEFSMT YKPVIHGRPG VGATHSSRFI PLK

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Macromolecule #5: 40S ribosomal protein S18, putative

MacromoleculeName: 40S ribosomal protein S18, putative / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 17.585469 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MSLTLQSEQF QHIVRLLNTN VEGKRKVPFA LRMVKGVGIR FAYMVCKKAG IDVERRAGTL TAEELERISE VIADPAKFKI PDWFLNRQR DPKTGKTEHL TSAMVDTRLR EDLERLRKNR VHRGVRHAYG LRVRGQHTCT SGRHGKTVGV SRTK

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Macromolecule #6: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 30.936521 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MTKKHLKRLY APKDWMLSKL TGVFAPRPRA GPHKLRECMT LMIIIRNRLK YALNAAEAQM ILRQGLVCVD GKPRKDTKYP VGFMDVVEI PRTGDRFRIL YDVKGRFALV KVGEAEGNIK LLKVENVYTS TGRIPVAMTH DGHRIRYPDP RTHRGDTLVY N LKEKKVVD ...String:
MTKKHLKRLY APKDWMLSKL TGVFAPRPRA GPHKLRECMT LMIIIRNRLK YALNAAEAQM ILRQGLVCVD GKPRKDTKYP VGFMDVVEI PRTGDRFRIL YDVKGRFALV KVGEAEGNIK LLKVENVYTS TGRIPVAMTH DGHRIRYPDP RTHRGDTLVY N LKEKKVVD LIKSSNGKVV MVTGGANRGR IGEIMSIERH PGAFDIARLK DAAGHEFATR ASNIFVIGKD MQSVPVTLPK QQ GLRINVI QEREEKLIAA EARKNMQTRG VRKARK

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Macromolecule #7: 40S ribosomal protein S23, putative

MacromoleculeName: 40S ribosomal protein S23, putative / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 15.960672 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MTKANGQHAA RKLVRLRRKN RWADKGWKKS HTFVAQKANP FGGSSHAKGI VLEKIGVGAK QPNSAIRKCV RVQLIKNDKK IIAFVPNDG CLHFIEENDE VLVSGFGRSG HAVGDIPGVR FKIVKVSNVG LYALYRQKKE KPRN

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Macromolecule #8: 40S ribosomal protein S5, putative

MacromoleculeName: 40S ribosomal protein S5, putative / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 21.292715 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MSAKAPKLFN KWSYENLQTT EIALSDHISK TATYVPHSAG RWQKKRFRKA RIPIVERLTN GLMFKGRGNG KKLQAVRLVK HTLEIIHLL TDQNPLQVVI DAVSKGAPRE DSTRVGSGGV VRRQAVDVSP MRRVNEAIYL MCKGAREAAF RNLKTLPECL A DEIVNASK GSSNSYAIKK KDEVERVAKA NR

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Macromolecule #9: Ribosomal protein S7, putative

MacromoleculeName: Ribosomal protein S7, putative / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 24.896504 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MAATRKGGRM SVKPHLRKLR KLKRPNPSED EDSVAKALFE LEATHKTLRT QLPRFHINTV RTLSSPRFRR TATVVFYPLR FLMLVRKIQ RTLTAELEKR FPGSIVVLVA QRKITKRPKD VYKLQKVQRS RTSVAVFENI LNDLIYPCDV MGRRWRCRTD G TKVMKVFL ...String:
MAATRKGGRM SVKPHLRKLR KLKRPNPSED EDSVAKALFE LEATHKTLRT QLPRFHINTV RTLSSPRFRR TATVVFYPLR FLMLVRKIQ RTLTAELEKR FPGSIVVLVA QRKITKRPKD VYKLQKVQRS RTSVAVFENI LNDLIYPCDV MGRRWRCRTD G TKVMKVFL DSRDRKRVES RLPIIAHVYK QLTHRTVSFG FMWNPKLQQV SSR

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Macromolecule #10: 40S ribosomal protein S13, putative

MacromoleculeName: 40S ribosomal protein S13, putative / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 17.295621 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MVRMQGVGHG KSASSLPFCR TPPSWLKIAS RDVIDAVCKL AKKGLTPSRI GMQLRDSMGI AQVKNVTGRK ILRILKHKGM APEIPEDLY CLIKRATEMR KHLERNTKDR DTKFRLILVE SRIHRLARYY KRVKQLPPTW KYESSTASAM VA

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Macromolecule #11: 40S ribosomal protein S27, putative

MacromoleculeName: 40S ribosomal protein S27, putative / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 9.581131 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MGFFDSDLSY PTVRSERMKH KRRRLVQGPN SYFMDVKCPG CNAITIVYSH ATSEVQCNGC ATKLCRPTGG KAMLTTGCGF RKKADH

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Macromolecule #12: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 12.793177 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MTTKRRNHGR AKPPCSRGRT RPVHCFNCGR LAPKDKAVGR FVVRRMLDQA SARDVAEASN VYGAGFPMPK LYMKQRFCIA CAIHSRTVR ARPTDMRKRR FTTKVPFRPT GAK

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Macromolecule #13: 40S ribosomal protein S33, putative

MacromoleculeName: 40S ribosomal protein S33, putative / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 12.427547 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MLASFSPLLF LCYCCVISSL VMTEVAATKK EKKAPQEEVV VQQTDQAQVG LIIKVLGRTG SRGNVTQVRV RLMAEEGSPD ANRTIVRNV KGPCKEGDML SLMETEREAR RLR

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Macromolecule #14: 40S ribosomal protein S14, putative

MacromoleculeName: 40S ribosomal protein S14, putative / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 15.596004 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MSKKQEVKFY GSSAAKDELV YGVVHIYASF NDTFVHVTDM SGRETFCKIT GGMKVKADRD ESSPYAAMMA AQDVVARCKE CGINALHVK MRATGGVGTR SPGPGAQAAL RALARAGMKI GRIEDVTPIP TDSTRRKGSR RGRRL

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Macromolecule #15: 40S ribosomal protein S3a-2

MacromoleculeName: 40S ribosomal protein S3a-2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 29.909693 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MTLGKNKRIS KGGKRGKKKA QETMSRKEWY DVVAPKNFEV RQFGKTICNK TQGTKIAADY LRGRVYESNL ADLNKTQGDD DAYRKVKFV VQEVQGRNLL TQFHSMEMTS DRVYFLLRKW CTTIEAAVEA KTADGYTLRL FVIAFTKKQS NQLSKNCYAK T RLVKWVRH ...String:
MTLGKNKRIS KGGKRGKKKA QETMSRKEWY DVVAPKNFEV RQFGKTICNK TQGTKIAADY LRGRVYESNL ADLNKTQGDD DAYRKVKFV VQEVQGRNLL TQFHSMEMTS DRVYFLLRKW CTTIEAAVEA KTADGYTLRL FVIAFTKKQS NQLSKNCYAK T RLVKWVRH RITNLIRQRL SKVNINEAVT LLTRNILRDR LAKRCNPIVP LRDLRIRKVK VVRTPRFDAQ ALLNAHGEIP AS AEGEARV VEEAQEAPAA EATA

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Macromolecule #16: 40S ribosomal protein S11, putative

MacromoleculeName: 40S ribosomal protein S11, putative / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 20.018307 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MTAPEQYARV HSVDLTLQHE KAYQRQTAVN ENSHNPNKKH VNMSGHIRYS KKIGLGFKTP KSAINGKYID RKCPFTSDVS IRGRILRGV VHSTKMKRSI VIRRDYLHFI RKYQRYQKRH RSLTVHCSPC FDPKPGDEVI VGQCRPLSKT IRYNVLEVVS K SAPGKLGR KFSKN

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Macromolecule #17: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 15.706745 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MVFQKKKAEV TVRTSQFKVN KLLNRKQFIV EVNHPGWCGT VPAKLIRSRL ASLYKVADEN QISVFGFKTK FGGGKTTGFG LIYDDLAAL KRIEPNYRKA RLGMGKKKQP ARKSVKERRN RNKKIRGKAK GKMQTKKK

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Macromolecule #18: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 25.246783 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MGIVRSRLHK RKITGGKTKI HRKRMKAELG RLPAHTKLGA RRVSPVRARG GNFKLRGLRL DTGNFAWSTE AIAQRARILD VVYNATSNE LVRTKTLVKN CIVVVDAAPF KLWYAKHYGI DLDAAKSKKT LQSTTEKKKS KKTSHAMTEK YDVKKASDEL K RKWMLRRE ...String:
MGIVRSRLHK RKITGGKTKI HRKRMKAELG RLPAHTKLGA RRVSPVRARG GNFKLRGLRL DTGNFAWSTE AIAQRARILD VVYNATSNE LVRTKTLVKN CIVVVDAAPF KLWYAKHYGI DLDAAKSKKT LQSTTEKKKS KKTSHAMTEK YDVKKASDEL K RKWMLRRE NHKIEKAVAD QLKEGRLLAR ITSRPGQTAR ADGALLEGAE LQFYLKKLEK KKR

+
Macromolecule #19: 40S ribosomal protein S9, putative

MacromoleculeName: 40S ribosomal protein S9, putative / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 22.181932 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MRNYNNFNRV WKAPRRPFEK ERLDREMKLC GQYGLRCKRE IWRVNMTLSK MRRTARLLLT LPENHPRRQL EGSAIMRRCH DYGFLEEEK DKLDYVLSLT VPDILERRLQ TVVFKHGLAK SVHHARVLIL QRHIAVAKQI VTIPSFIVRV SSEHHIAFAD A SPFGNGRP GRVKRVKRKA AKKGAAGGDD DE

+
Macromolecule #20: 40S ribosomal protein SA

MacromoleculeName: 40S ribosomal protein SA / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 27.885129 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MTSVESGAKV LRMKEGDVQK LVAMHCHLGT KNRSNAMKKY IHSRTKEGTN IIDLHMTWEK LILAARVIAA VENPQDVTVC STRLFGQRA IFKFSQLVGT SFLAGRFIPG TFTNQIQKKF MQPRVLLVTD PRTDHQALRE ASLVNIPVIA FCDTDAPLEF V DIAIPCNN ...String:
MTSVESGAKV LRMKEGDVQK LVAMHCHLGT KNRSNAMKKY IHSRTKEGTN IIDLHMTWEK LILAARVIAA VENPQDVTVC STRLFGQRA IFKFSQLVGT SFLAGRFIPG TFTNQIQKKF MQPRVLLVTD PRTDHQALRE ASLVNIPVIA FCDTDAPLEF V DIAIPCNN RGRYSISMMY WLLAREVLRL RGTIPRSVPW DVKVDLFFYR DPEEALKHEE VNQAAAPVAE ADEGFGWVER DN NAWEQ

+
Macromolecule #21: 40S ribosomal protein S2, putative

MacromoleculeName: 40S ribosomal protein S2, putative / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 28.574951 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MADAQRAPSG EQRGFGRGRG GRGGRGGRGG RGGSNEKEEW VPCTKLGRLV KEQKITSLEE IFLFSMPIKE HQIVDQLIKE SDMRDELML IVPVQKATSA GQRTRFKAFN IVGDGNGHIG VGARVGKEVS LAIRASMIAA KLNVVPIRRG YWGNKIGEPH T IPMKVTGK ...String:
MADAQRAPSG EQRGFGRGRG GRGGRGGRGG RGGSNEKEEW VPCTKLGRLV KEQKITSLEE IFLFSMPIKE HQIVDQLIKE SDMRDELML IVPVQKATSA GQRTRFKAFN IVGDGNGHIG VGARVGKEVS LAIRASMIAA KLNVVPIRRG YWGNKIGEPH T IPMKVTGK CGSVSVRLIP APRGAGIVAA PVPKKILEFA GVEDVYTSSC GNTRTRGNFI MATFYALRKT YGFLTPDLWA ET EVSRDPT DEHSDFLTMG SKMIAA

+
Macromolecule #22: 40S ribosomal protein S15a, putative

MacromoleculeName: 40S ribosomal protein S15a, putative / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 14.68327 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MTMMSVLANA LRCIASAERR GKRQVLIRPS SKVVVKFLQV MQKHGYIGEF EIIDDHRAGK IVVNLNGRLN KCGAICPRFD CTTPDFEKW VKNILPSRQF GFVVLTTSLG IMDHEEARAR NTGGKVLGFF Y

+
Macromolecule #23: 40S ribosomal protein S21, putative

MacromoleculeName: 40S ribosomal protein S21, putative / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 26.09457 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MTTIGTYNEE GVNVDLYIPR KCHATNNLIT SYDHSAVQIA IANVDANGVL NGTTTTFCIA GYLRRQAESD HAINHLAISK GIIRIKTGK KPRAKKLKNV KGLGVRGLPR GALQQRGARV LPTQRGVAQR GGAQKGNVRK LQPQPQKQRS QLNQRSQQQH G ARPTRKEE ...String:
MTTIGTYNEE GVNVDLYIPR KCHATNNLIT SYDHSAVQIA IANVDANGVL NGTTTTFCIA GYLRRQAESD HAINHLAISK GIIRIKTGK KPRAKKLKNV KGLGVRGLPR GALQQRGARV LPTQRGVAQR GGAQKGNVRK LQPQPQKQRS QLNQRSQQQH G ARPTRKEE GGRTQRGGRD APQARKQQGR NEPQARKQQG RDAPQARKQQ GRDAPQARKQ QGRDAPQARN KKSGPNRK

+
Macromolecule #24: Ribosomal protein S25, putative

MacromoleculeName: Ribosomal protein S25, putative / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 12.379955 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MPPKKNAKPT KPAPKKGKME NMNKGAKKAA KKWSKGRTRE ALQNAVVFDK ETMDKLLSEV PKYKVITPSI ISDRLKISVA LAAKGLQHL CRQKLIKLVS CSSKFRLYSR V

+
Macromolecule #25: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 15.79211 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MAEETPVLEV SPAVVDAVMD ATPENLEDAL RIVLQKSREV NGLIRGLSEV ARALDRRTAH LCVLAEDCED EEYKKLITAL AKQGEVDLI NVEEREKLAQ WAGLTKMDST GELKKTFKCS CVAVRDFGER TRALEFLLSH LR

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Macromolecule #26: Ubiquitin/ribosomal protein S27a, putative

MacromoleculeName: Ubiquitin/ribosomal protein S27a, putative / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 16.608434 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MQIFVKSAAG KTSAIQASAQ ETVASLKSKA GVCDGTLFYG GHCLCDEATL ADYGLQREST IQVMLPVEGG KGKKKKKKVF TKPKKPIHR HKLEKMRALK YFKVTENDDG SFKVERTRDE CPNPVCGAGV FMAQHKDRKY CGKCHLTYAA K

+
Macromolecule #27: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 28.344391 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MKLNIAYPRN GTVKQFEITE EVLRRVSLTD YRLGNEVDGA IFGDAFRGYT FKLQGGSDKQ GFPMVQGVMA PSRVSLLVKR GAVGFNTFR GYQGERRRKS LRGCILASDI AVLNVVIVKT GEQPIEGVTD VSVPRRLGPK RANKIRKLFN LSRGDDVRKY V IRRKVVKE ...String:
MKLNIAYPRN GTVKQFEITE EVLRRVSLTD YRLGNEVDGA IFGDAFRGYT FKLQGGSDKQ GFPMVQGVMA PSRVSLLVKR GAVGFNTFR GYQGERRRKS LRGCILASDI AVLNVVIVKT GEQPIEGVTD VSVPRRLGPK RANKIRKLFN LSRGDDVRKY V IRRKVVKE GKKDRFKAPK IQRLITTAVK ARRAKKAKVA IEKVRKSAEE RRAYLHLIGT RRRAARQRNS ARRHAHKVAA QK QALALFK GKK

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Macromolecule #28: 40S ribosomal protein S17, putative

MacromoleculeName: 40S ribosomal protein S17, putative / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 22.680713 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MCMQHHTCAL FFASCTAFIC FHWLGCTFGC RSFLFFFTFG FFSKREERVQ SNTASMGKIR TKTVKRASKQ LVEKYFSKLN KDFYQNKRV VMDVTIARSK KLKNKIAGYT THIMKRLARG PVRGISLKLQ EEERERRMDY VPEVSHVDQA IQDGVHVDKQ T FSMLKRME ...String:
MCMQHHTCAL FFASCTAFIC FHWLGCTFGC RSFLFFFTFG FFSKREERVQ SNTASMGKIR TKTVKRASKQ LVEKYFSKLN KDFYQNKRV VMDVTIARSK KLKNKIAGYT THIMKRLARG PVRGISLKLQ EEERERRMDY VPEVSHVDQA IQDGVHVDKQ T FSMLKRME TGVPRHVALT TVVAPVTKKG GRRAPARK

+
Macromolecule #29: Ribosomal protein S20, putative

MacromoleculeName: Ribosomal protein S20, putative / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 13.248478 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MAYVKKDQAP AQETQRVLVK MTSRNAKAVE NVMTELLLHA RNEKVVLRGP VRLPTRTLKV TTRKTPCGNG TNTWDTYELK IYKRLVELR APTELVKKIT SFPIDAGVDV AITIPHDQ

+
Macromolecule #30: 40S ribosomal protein S3, putative

MacromoleculeName: 40S ribosomal protein S3, putative / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 24.188436 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MGPLSKKRMI IRDGVFYAEL FEFLKRELAD DGFAGVEHRV TSTRTEIVIR ATKTREVLGE KGRRIRELTA CLQQRFHYKE GKLQLFAER VEVRGLSAMA QAESLRFKLL SNLQVRRAAM GIIRHVMESG AKGCEVTVGG KIKGQRAKSM TFRDGYMIKS G TAHKNFVD ...String:
MGPLSKKRMI IRDGVFYAEL FEFLKRELAD DGFAGVEHRV TSTRTEIVIR ATKTREVLGE KGRRIRELTA CLQQRFHYKE GKLQLFAER VEVRGLSAMA QAESLRFKLL SNLQVRRAAM GIIRHVMESG AKGCEVTVGG KIKGQRAKSM TFRDGYMIKS G TAHKNFVD VATRHCHLRA GTLGVKVKIM LPKSMQNEEE ILPDVITVLE PKEITA

+
Macromolecule #31: 40S ribosomal protein S10, putative

MacromoleculeName: 40S ribosomal protein S10, putative / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 18.284725 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MATYVPKRSR DDVYRFFFTE GVISCKKDPL GTWTGSLGGK TFTVPSIQVM QLMRSMKSRG LIKEQFAWRH FYWFLNDEGV EYLRKYLFL PHDAVPNTHK AEYKVLEREG GRGRGRGEGR GRGRGEGRGR GRGEGRGRGR GFHSERDAYR TGAAAAPAVE A Q

+
Macromolecule #32: Ribosomal protein S19, putative

MacromoleculeName: Ribosomal protein S19, putative / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 18.861287 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MVAARTKANS KIRRIGKKKG ATMKDVHPWR WVKLCSQHFK QEGKLMVPNC TEIVKSSHGR ERAPQNPDWY YIRCAAILRA VYMRPGVGY GGLSKRFSNK KNRGSRPEIT VRASKGLLHW GCKSLTKLGL IEKCESSGHR ITKNGRKLAD AIAFKVAIRK L NTATSKN

+
Macromolecule #33: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 7.445938 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString:
MGKVHGSLAR AGKVKNQTPK VAKQEKKKQP RGRAFKRLKY TQRYLAKTLK PGEKLHMNKQ PPGKAG

+
Macromolecule #34: RNA-binding protein, putative

MacromoleculeName: RNA-binding protein, putative / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
Molecular weightTheoretical: 27.676303 KDa
Recombinant expressionOrganism: Trypanosoma cruzi (eukaryote)
SequenceString: MPAKSANKPA SKPASKPAAK PAAKPAAKAP APKAEKKGAA KAPAPKAAAP APKAAAAAPK PAVRDAKQRS DAANHNGLYV KNWGQGSVD DARALFGTAG KVVGVRVRRR RYAIIFFENA AAVKKAIDLF NGKEFMGNVL SVVPAKTTPK PDPHANSSVV F VSPIFRAS ...String:
MPAKSANKPA SKPASKPAAK PAAKPAAKAP APKAEKKGAA KAPAPKAAAP APKAAAAAPK PAVRDAKQRS DAANHNGLYV KNWGQGSVD DARALFGTAG KVVGVRVRRR RYAIIFFENA AAVKKAIDLF NGKEFMGNVL SVVPAKTTPK PDPHANSSVV F VSPIFRAS TTKKQILELF SGMKVLRLRT YRNNYAYVYL DTPAAAQRAV KEKNGAEFRG KQLRVALSTR SLAKDRARAE RA RLLMAAQ KFNKRKNHTK

+
Macromolecule #35: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 746.696125 KDa
SequenceString: UGAUCUGGUU GAUUCUGCCA GUAGUCAUAU GCUUGUUUCA AGGACUUAGC CAUGCAUGCC UCAGAAUCAC UGCAUUGCAG GAAUCUGCG CAUGGCUCAU UACAUCAGAC GUAAUCUGCC GCAAAAAUCU UGCGGUCUCC GCAACAUUGG AUAACUUGGC G AAACGCCA ...String:
UGAUCUGGUU GAUUCUGCCA GUAGUCAUAU GCUUGUUUCA AGGACUUAGC CAUGCAUGCC UCAGAAUCAC UGCAUUGCAG GAAUCUGCG CAUGGCUCAU UACAUCAGAC GUAAUCUGCC GCAAAAAUCU UGCGGUCUCC GCAACAUUGG AUAACUUGGC G AAACGCCA AGCUAAUACA UGAACCAACC GGAUGUUCUC UGUUCCGGCG GCAGGGCAAC CUGCUGCCAU GGGACGUCCA GC GAAUGAA UGAAAGUAAA ACCAAUGCCU UCACCGGCAG UAACACUCAG AAGUGUUGAU UCAAUUCAUU CCGUGCGAAA GCC GGGUUU UUUUAUCCGG CGUCUUUUGA CGAACAACUG CCCUAUCAGC CAGCGAUGGC CGUGUAGUGG ACUGCCAUGG CGUU GACGG GAGCGGGGGA UUAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAAUAGCUA CCACUUCUAC GGAGGGCAGC AGGCG CGCA AAUUGCCCAA UGUCAAAAAA AAAAGAUGAG GCAGCGAAAA GAAAUAGAGC CGACAGUGCU UUUGCAUUGU CGUUUU CAA UGGGGGAUAU UUAAACCCAU CCAAAAUCGA GUAACAAUUG GAGGACAAGU CUGGUGCCAG CACCCGCGGU AAUUCCA GC UCCAAAAGCG UAUAUUAAUG CUGUUGCUGU UAAAGGGUUC GUAGUUGAAU UGAGGGCCUC UAAGGCGCAA UGGUUUAG U CCCAUCCACU UCGGAUUGGU GACCCAUGCC CUUGUGGUCC GUGAACAGAC AUUCAGAAAC AAAAAACACG GGAGUGGUA CCUUUCCUGA UUAUCGCAUG UCAUGCAUGC CAGAGGGCGC CCGUGAUUUU UUACUGUGAC UAAAAAAGUG UGACCAAAGC AGUCAUUCG ACUUGAAUUA GAAAGCAUGG GAUAACAAAG GAGCAGCCUC UGGGCCACCG UUUCGGCUUU UGUUGGUUUU A AAAGUCCA UUGGAGAUUA UGGGGCAGUG UGACAAGCGG CUGGGUGGUU AUUCCACACA CACACACACA CGCUCCUUUU UU UUGGACG UGUUUUGUGU GUGUAUGUGG CACUCGUCGC CUUUGUGGGA AAUCCGUGUG GCACUGUGUU UGAUGUUGUU GGC AGAGAC UUCGGUCUUU UGCCUUCGCA UAUUUCACAC AUGUGUCAUG CCUUCCCUCA ACUCACGGCA UCCAGGAAUG AAGG AGGGU AGUUCGGGGG AGAACGUACU GGUGCGUCAG AGGUGAAAUU CUUAGACCGC ACCAAGACGA ACUACAGCGA AGGCA UUCU UCAAGGAUAC CUUCCUCAAU CAAGAACCAA AGUGUGGGGA UCGAAGAUGA UUAGAGACCA UUGUAGUCCA CACUGC AAA CGAUGACACC CAUGAAUUGG GGAGUUUUUG GUCGUAGGCG UGGUCGGGCU UGAUUAUUAU UUUUCAUCCC GUUCCUC GU CUCGCCAAUG AAUAUUAAAU UUACGUGCAU AUUCUUUUUG GUCUUCGUUU UUUUACGGCG AGGGCCUUUA ACGGGAAU A UCCUCAGCAC GUUAUCUGAC UUCUUCACGC GAAAGCUUUG AGGUUACAGU CUCAGGGGGG AGUACGUUCG CAAGAGUGA AACUUAAAGA AAUUGACGGA AUGGCACCAC AAGACGUGGA GCGUGCGGUU UAAUUUGACU CAACACGGGG AACUUUACCA GAUCCGGAC AGGGUGAGGA UUGACAGAUU GAGUGUUCUU UCUCGAUCCC CUGAAUGGUG GUGCAUGGCC GCUUUUGGUC G GUGGAGUG AUUUGUUUGG UUGAUUCCGU CAACGGACGA GAUCCAAGCU GCCCAGUAGG AUUCAGAAUU GCCCAUAGGA UA GCAAUCC CUUCCGCGGG UUUUACCCAA GGGGGGGCGG UAUUCGCUUG UAUCCUUCUC UGCGGGAUUC CUUGUUUUGC GCA AGGUGA GAUUUUGGGC AACAGCAGGU CUGUGAUGCU CCUCAAUGUU CUGGGCGACA CGCGCACUAC AAUGUCAGUG AGAA CAAGA AAAACGACUC UUGUCGGACC UACUUGAUCA AAAGAGUGGG AAAACCCCGG AAUCACGUAG ACCCACUUGG GACCG AGUA UUGCAAUUAU UGGUCGCGCA ACGAGGAAUG UCUCGUAGGC GCAGCUCAUC AAACUGUGCC GAUUACGUCC CUGCCA UUU GUACACACCG CCCGUCGUUG UUUCCGAUGA UGGUGCAAUA CAGGUGAUCG GACAGUCGAG UGCUUCACUU GACCGAA AG UUCACCGAUA UUUCUUCAAU AGAGGAAGCA AAAGUCGUAA CAAGGUAGCU GUAGGUGAAC CUGCAGCUGG AUCAUUU

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 2.2 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 86000

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