EMDB-3581: Structure of 70S ribosome from Lactococcus lactis

Basic information

• Entry: Database: EMDB / ID: EMD-3581
• Title: Structure of 70S ribosome from Lactococcus lactis

Sample

• Complex: 70S ribosomeRibosome
  • RNA: x 3 types
  • Protein or peptide: x 48 types

Function / homology

Function:

primary metabolic process / ribosomal large subunit assembly / large ribosomal subunit / regulation of translation / small ribosomal subunit / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / mRNA binding / zinc ion binding / cytoplasm

Domain/homology:

Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / Ribosomal protein L31 type B / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein S14, type Z / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein S21 / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal protein S15, bacterial-type / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily

Component:

50S ribosomal protein L32 / 50S ribosomal protein L34 / 50S ribosomal protein L28 / 30S ribosomal protein S21 / 30S ribosomal protein S4 / Ribosome hibernation promotion factor / 50S ribosomal protein L31 type B / 30S ribosomal protein S16 / 50S ribosomal protein L27 / 50S ribosomal protein L21 / 50S ribosomal protein L19 / 30S ribosomal protein S20 / 50S ribosomal protein L20 / 50S ribosomal protein L35 / 30S ribosomal protein S15 / 50S ribosomal protein L17 / 30S ribosomal protein S11 / 30S ribosomal protein S13 / 50S ribosomal protein L36 / 50S ribosomal protein L15 / 50S ribosomal protein L30 / 30S ribosomal protein S5 / 50S ribosomal protein L18 / 50S ribosomal protein L6 / 30S ribosomal protein S8 / 30S ribosomal protein S14 type Z / 50S ribosomal protein L5 / 50S ribosomal protein L24 / 50S ribosomal protein L14 / 30S ribosomal protein S17 / 50S ribosomal protein L29 / 50S ribosomal protein L16 / 30S ribosomal protein S3 / 50S ribosomal protein L22 / 30S ribosomal protein S19 / 50S ribosomal protein L2 / 50S ribosomal protein L23 / 50S ribosomal protein L4 / 50S ribosomal protein L3 / 30S ribosomal protein S10 / 50S ribosomal protein L33 3 / 30S ribosomal protein S2 / 30S ribosomal protein S18 / 30S ribosomal protein S6 / 30S ribosomal protein S9 / 50S ribosomal protein L13 / 30S ribosomal protein S7 / 30S ribosomal protein S12

• Biological species: Lactococcus lactis (lactic acid bacteria) / Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria) / Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 5.6 Å
• Authors: Franken LE / Oostergetel GT / Pijning T / Puri P / Boekema EJ / Poolman B / Guskov A
• Citation: Journal: Nat Commun / Year: 2017; Title: A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy.; Authors: Linda E Franken / Gert T Oostergetel / Tjaard Pijning / Pranav Puri / Valentina Arkhipova / Egbert J Boekema / Bert Poolman / Albert Guskov / ; Abstract: Bacteria downregulate their ribosomal activity through dimerization of 70S ribosomes, yielding inactive 100S complexes. In Escherichia coli, dimerization is mediated by the hibernation promotion factor (HPF) and ribosome modulation factor. Here we report the cryo-electron microscopy study on 100S ribosomes from Lactococcus lactis and a dimerization mechanism involving a single protein: HPF. The N-terminal domain of HPF binds at the same site as HPF in Escherichia coli 100S ribosomes. Contrary to ribosome modulation factor, the C-terminal domain of HPF binds exactly at the dimer interface. Furthermore, ribosomes from Lactococcus lactis do not undergo conformational changes in the 30S head domains upon binding of HPF, and the Shine-Dalgarno sequence and mRNA entrance tunnel remain accessible. Ribosome activity is blocked by HPF due to the inhibition of mRNA recognition by the platform binding center. Phylogenetic analysis of HPF proteins suggests that HPF-mediated dimerization is a widespread mechanism of ribosome hibernation in bacteria.When bacteria enter the stationary growth phase, protein translation is suppressed via the dimerization of 70S ribosomes into inactive complexes. Here the authors provide a structural basis for how the dual domain hibernation promotion factor promotes ribosome dimerization and hibernation in bacteria.

History

Deposition	Jan 26, 2017	-
Header (metadata) release	Feb 15, 2017	-
Map release	Oct 11, 2017	-
Update	Oct 11, 2017	-
Current status	Oct 11, 2017	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_3581.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.105 Å

Density

Contour Level	By AUTHOR: 0.035 / Movie #1: 0.035
Minimum - Maximum	-0.0636561 - 0.16130547
Average (Standard dev.)	0.00072141824 (±0.006043833)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 600 600 600 Spacing 600 600 600
Cell	A=B=C: 663.0 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.105	1.105	1.105
M x/y/z	600	600	600
origin x/y/z	0.000	0.000	0.000
length x/y/z	663.000	663.000	663.000
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	600	600	600
D min/max/mean	-0.064	0.161	0.001

Supplemental data

Supplemental map: emd 3581 additional.map

• File: emd_3581_additional.map

Sample components

Entire : 70S ribosome

• Entire: Name: 70S ribosomeRibosome

Components

• Complex: 70S ribosomeRibosome
  • RNA: 16S ribosomal RNA
  • Protein or peptide: 30S ribosomal protein S2
  • Protein or peptide: 30S ribosomal protein S3
  • Protein or peptide: 30S ribosomal protein S4
  • Protein or peptide: 30S ribosomal protein S5
  • Protein or peptide: 30S ribosomal protein S6
  • Protein or peptide: 30S ribosomal protein S7
  • Protein or peptide: 30S ribosomal protein S8
  • Protein or peptide: 30S ribosomal protein S9
  • Protein or peptide: 30S ribosomal protein S10
  • Protein or peptide: 30S ribosomal protein S11
  • Protein or peptide: 30S ribosomal protein S12
  • Protein or peptide: 30S ribosomal protein S13
  • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
  • Protein or peptide: 30S ribosomal protein S15
  • Protein or peptide: 30S ribosomal protein S16
  • Protein or peptide: 30S ribosomal protein S17
  • Protein or peptide: 30S ribosomal protein S18
  • Protein or peptide: 30S ribosomal protein S19
  • Protein or peptide: 30S ribosomal protein S20
  • Protein or peptide: 30S ribosomal protein S21
  • Protein or peptide: 50S ribosomal protein L28
  • Protein or peptide: 50S ribosomal protein L29
  • Protein or peptide: 50S ribosomal protein L30
  • Protein or peptide: 50S ribosomal protein L31 type BRibosome
  • Protein or peptide: 50S ribosomal protein L32
  • Protein or peptide: 50S ribosomal protein L33 3Ribosome
  • Protein or peptide: 50S ribosomal protein L34
  • Protein or peptide: 50S ribosomal protein L35
  • Protein or peptide: 50S ribosomal protein L36
  • RNA: 23S ribosomal RNA
  • RNA: 5S ribosomal RNA
  • Protein or peptide: 50S ribosomal protein L2
  • Protein or peptide: 50S ribosomal protein L3
  • Protein or peptide: 50S ribosomal protein L4
  • Protein or peptide: 50S ribosomal protein L5
  • Protein or peptide: 50S ribosomal protein L6
  • Protein or peptide: 50S ribosomal protein L13
  • Protein or peptide: 50S ribosomal protein L14
  • Protein or peptide: 50S ribosomal protein L15
  • Protein or peptide: 50S ribosomal protein L16
  • Protein or peptide: 50S ribosomal protein L17
  • Protein or peptide: 50S ribosomal protein L18
  • Protein or peptide: 50S ribosomal protein L19
  • Protein or peptide: 50S ribosomal protein L20
  • Protein or peptide: 50S ribosomal protein L21
  • Protein or peptide: 50S ribosomal protein L22
  • Protein or peptide: 50S ribosomal protein L23
  • Protein or peptide: 50S ribosomal protein L24
  • Protein or peptide: 50S ribosomal protein L27
  • Protein or peptide: Ribosome hibernation promotion factor

Supramolecule #1: 70S ribosome

• Supramolecule: Name: 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51
• Source (natural): Organism: Lactococcus lactis (lactic acid bacteria)

Macromolecule #1: 16S ribosomal RNA

• Macromolecule: Name: 16S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
• Molecular weight: Theoretical: 497.142344 KDa

Sequence

String:

UUGAGAGUUU GAUCCUGGCU CAGGACGAAC GCUGGCGGCG UGCCUAAUAC AUGCAAGUUG AGCGAUGAAG AUUGGUGCUU GCACCAAUU UGAAGAGCAG CGAACGGGUG AGUAACGCGU GGGGAAUCUG CCUUUGAGCG GGGGACAACA UUUGGAAACG A AUGCUAAU ACCGCAUAAU AACUUUAAAC AUAAGUUUUA AGUUUGAAAG AUGCAAUUGC AUCACUCAAA GAUGAUCCCG CG UUGUAUU AGCUAGUUGG UGAGGUAAAG GCUCACCAAG GCGAUGAUAC AUAGCCGACC UGAGAGGGUG AUCGGCCACA UUG GGACUG AGACACGGCC CAAACUCCUA CGGGAGGCAG CAGUAGGGAA UCUUCGGCAA UGGACGAAAG UCUGACCGAG CAAC GCCGC GUGAGUGAAG AAGGUUUUCG GAUCGUAAAA CUCUGUUGGU AGAGAAGAAC GUUGGUGAGA GUGGAAAGCU CAUCA AGUG ACGGUAACUA CCCAGAAAGG GACGGCUAAC UACGUGCCAG CAGCCGCGGU AAUACGUAGG UCCCGAGCGU UGUCCG GAU UUAUUGGGCG UAAAGCGAGC GCAGGUGGUU UAUUAAGUCU GGUGUAAAAG GCAGUGGCUC AACCAUUGUA UGCAUUG GA AACUGGUAGA CUUGAGUGCA GGAGAGGAGA GUGGAAUUCC AUGUGUAGCG GUGAAAUGCG UAGAUAUAUG GAGGAACA C CGGUGGCGAA AGCGGCUCUC UGGCCUGUAA CUGACACUGA GGCUCGAAAG CGUGGGGAGC AAACAGGAUU AGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA GAUGUAGGGA GCUAUAAGUU CUCUGUAUCG CAGCUAACGC AAUAAGCACU CCGCCUGGG GAGUACGACC GCAAGGUUGA AACUCAAAGG AAUUGACGGG GGCCCGCACA AGCGGUGGAG CAUGUGGUUU A AUUCGAAG CAACGCGAAG AACCUUACCA GGUCUUGACA UACUCGUGCU AUUCCUAGAG AUAGGAAGUU CCUUCGGGAC AC GGGAUAC AGGUGGUGCA UGGUUGUCGU CAGCUCGUGU CGUGAGAUGU UGGGUUAAGU CCCGCAACGA GCGCAACCCC UAU UGUUAG UUGCCAUCAU UAAGUUGGGC ACUCUAACGA GACUGCCGGU GAUAAACCGG AGGAAGGUGG GGAUGACGUC AAAU CAUCA UGCCCCUUAU GACCUGGGCU ACACACGUGC UACAAUGGAU GGUACAACGA GUCGCGAGAC AGUGAUGUUU AGCUA AUCU CUUAAAACCA UUCUCAGUUC GGAUUGUAGG CUGCAACUCG CCUACAUGAA GUCGGAAUCG CUAGUAAUCG CGGAUC AGC ACGCCGCGGU GAAUACGUUC CCGGGCCUUG UACACACCGC CCGUCACACC ACGGGAGUUG GGAGUACCCG AAGUAGG UU GCCUAACCGC AAGGAGGGCG CUUCCUAAGG UAAGACCGAU GACUGGGGUG AAGUCGUAAC AAGGUAGCCG UAUCGGAA G GUGCGGCUGG AUCA

Macromolecule #31: 23S ribosomal RNA

• Macromolecule: Name: 23S ribosomal RNA / type: rna / ID: 31 / Number of copies: 1
• Source (natural): Organism: Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
• Molecular weight: Theoretical: 938.591125 KDa

Sequence

String:

CAAAGUUAAU AAGGGCGCAC GGUGGAUGCC UUGGCACUAA GAGCCGAUGA AGGACGUGAC UAACGACGAU AUUCUAGGGG GAGCAGUAA GUACGCAUUG AUCCCUAGGU CUCCGAAUGG GGAAACCCAG CUGUUACUAG CAGUUAUUCA UGAGUGAAUA C AUAGCUCA UGUAAAGGUA ACGCAGAGAA CUGAAACAUC UAAGUACCUG CAGGAAGAGA AAGUAAAAAC GAUUUCGUAA GU AGCGGCG AGCGAACGCG AAGAAGGGCA AACCAAGAAG CUUGCUUCUU GGGGUUGUAG GACUGCAACG UGGACUUAAG CAU UAUAGU CGAAUAACCU GGGAAGGUUA AUCAAAGAGG GUAAUAAUCC CGUAGACAAA AUAGCGCUUA UACCUAGCAG UAUC CUGAG UAGGGCUGGA CACGCGAAAU CCAGUUUGAA UCCGGGAGGA CCAUCUCCCA ACCCUAAAUA CUCCUUAGUG ACCGA UAGU GAACCAGUAC CGUGAGGGAA AGGUGAAAAG AACCCCGAGA GGGGAGUGAA AUAGCACCUG AAACCGUGUG CCUACA AGA AGUUCGAGCC CGUUAAUGGG UGAGAGCGUG CCUUUUGUAG AAUGAACCGG CGAGUUACGU UAUGAUGCGA GGUUAAG UU GAAGAGACGG AGCCGUAGGG AAACCGAGUC UGAAUAGGGC GACUUAGUAU CAUGAUGUAG ACCCGAAACC UAGUGACC U AUCCAUGAGC AGGGUGAAGG UGUGGUAAGA CGCACUGGAG GCCCGAACCA GGACACGUUG AAAAGUGUUU GGAUGACUU GUGGAUAGCG GAGAAAUUCC AAACGAACUG GGAGAUAGCU GGUUCUCUCC GAAAUAGCUU UAGGGCUAGC GUCGAAAUGU AAGUGUAUU GGAGGUAGAG CACUGUUUGG GUGAGGGGUC CGUCUAGGAU UACCAAUCUC AGAUAAACUC CGAAUGCUAA U ACACAUGU UCGGCAGUCA GACUGCGAGU GCUAAGAUCC GUAGUCGAAA GGGAAACAGC CCAGACCAAC AGCUAAGGUC CC AAAAUAU AUGUUAAGUG GAAAAGGAUG UGGGGUUGCA CAGACAACUA GGAUGUUAGC UCAGAAGCAG CUAUCAUUCA AAG AGUGCG UAAUAGCUCA CUAGUCGAGU GACCCUGCGC CGAAAAUGUA CCGGGGCUAA ACAUAUUACC GAAGCUUUGG AUUG AUAUU UUAUCAAUGG UAGGAGAGCG UUCUUAACCG CAAUGAAGGU AUACCGUGAG GAGUGCUGGA GCGUUAAGAA GUGAG AAUG CCGGUAUGAG UAGCGCAAGA UAAGUGAGAA UCUUAUCCAC CGUAAGACUA AGGUUUCCAG GGGAAGGCUC GUCCGC CCU GGGUUAGUCG GGACCUAAGG CGAGGCCGAA AGGCGUAGUC GAUGGACAAC UGGUUGAUAU UCCAGUACUA GAUAUGA UC GUGAUGGAGG GACGCAGUAG GCUAAGAGAU GCCAGUUAAU GGAUCCUGGU CUAAGCAGUG AGGUGUGAGA UGUGUCAA A UGCAUUUCUC UCUAACAUUG AGCUGUGAUG GGGAAGCAAC UACGGUUGCG AACUCUCUGA UGUCACACUG CCAAGAAAA GCUUCUAGCG UAAAGUCAUA UCUACCCGUA CCGCAAACCG ACACAGGUAG UCGAGGCGAG UAGCCUCAGG UGAUCGAGAG AACUCUCGU UAAGGAACUC GGCAAAAUAG CCCCGUAACU UCGGGAGAAG GGGUGCUGGU GUAAAAGCCA GCCGCAGUGA A UAGGCCCA AGCAACUGUU UAUCAAAAAC ACAGCUCUCU GCUAAACCGC AAGGUGAUGU AUAGGGGGUG ACGCCUGCCC GG UGCUGGA AGGUUAAGAG GAGUGCUUAG ACGUAAGUCG AAGGUAUGAA UUGAAGCCCC AGUAAACGGC GGCCGUAACU AUA ACGGUC CUAAGGUAGC GAAAUUCCUU GUCGGGUAAG UUCCGACCCG CACGAAAGGC GUAAUGAUUU GGGCACUGUC UCAA CGAGA GACUCGGUGA AAUUUUAGUA CCUGUGAAGA UGCAGGUUAC CCGCGACAGG ACGGAAAGAC CCCAUGGAGC UUUAC UGUA GUUUGAUAUU GAGUACCUGU AAGUCAUGUA CAGGAUAGGU AGGAGCCAUU GAAAUAGGGA CGCUAGUUUC UAUUGA GGC GUUGUUGGGA UACUACCCUU GACUUAUGGU UACUCUAACC CGCUGGCAUA AUCGGCCAGG GAGACAGUGU CUGACGG AC AGUUUGACUG GGGCGGUCGC CUCCUAAAGA GUAACGGAGG CGCUCAAAGG UUGGCUCAGA UUGGUUGGAA AUCAAUCG U AGAGUGUAAA GGUAAAAGCC AGCUUGACUG CGAGAGCUAC AACUCGAGCA GGUAGGAAAC UAGGACUUAG UGAUCCGGU GGUACCGCAU GGAAGGGCCA UCGCUCAACG GAUAAAAGCU ACCCUGGGGA UAACAGGCUU AUCUCCCCCA AGAGUUCACA UCGACGGGG AGGUUUGGCA CCUCGAUGUC GGCUCGUCGC AUCCUGGGGC UGUAGUCGGU CCCAAGGGUU GGGCUGUUCG C CCAUUAAA GCGGCACGCG AGCUGGGUUC AGAACGUCGU GAGACAGUUC GGUCCCUAUC CGUCGCGGGC GUAGGUAAUU UG AGAGGAU CUGUCCUUAG UACGAGAGGA CCGGGAUGGA CUUACCGCUG GUGUACCAGU UGUUCCGCCA GGAGCACGGC UGG AUAGCU AUGUAGGGAA GGGAUAAGCG CUGAAAGCAU CUAAGUGCGA AGCCCACCUC AAGAUGAGAU UACCCAUUCG UAAG AAUUA AGAGCCCAGA GAGAUGAUCU GGUAGAUAGG CUGGAAGUGG AAGAGUUGCG AGACUUGGAG CGGACCAGUA CUAAU CGCU CGAGGACUUU ACC

Macromolecule #32: 5S ribosomal RNA

• Macromolecule: Name: 5S ribosomal RNA / type: rna / ID: 32 / Number of copies: 1
• Source (natural): Organism: Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
• Molecular weight: Theoretical: 37.065969 KDa

Sequence

String:

UUGGUAUUAA UUGCAUAGGA GAUAUACCUG UUCCCAUGUC GAACACAGAA GUCAAGUCCU AUUGCGCUGG AAGUACUUGG GGGUUGCCC CCUGGGAGAU AAAGACGAUG CCAAGU

Macromolecule #2: 30S ribosomal protein S2

• Macromolecule: Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 28.58976 KDa

Sequence

String:

MSVISMKQLL EAGVHFGHQT RRWNPKMKPY IFTERNGIHV IDLQKTVKLV DDAYNYVKNA SQEGAVVLFV GTKKQAAEAV KEEALRAGQ YYVNHRWLGG MLTNWNTIQT RVTRLKEINK MEEEGTFEVL PKKEVVLLNK ERERLEKFIG GIADMPRIPD V MYIVDPHA EQIAVKEAKT LGIPVVAMVD TNADPEPIDV VIPANDDAIR AVKLITAKMA DAIIEGRQGE DAAEDFVAEE AA SEESLEE LAEIVEGK

Macromolecule #3: 30S ribosomal protein S3

• Macromolecule: Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 24.058795 KDa

Sequence

String:

MGQKVHPIGM RVGVIRDWDA KWYAEKEYAD YLHEDLAIRQ LIQTKLADAS VSLIETERAI NKVIVTLHTA KPGMVIGKSG ANVDALRAE LNKLTGKQVH INIVEIKKPD LDAHLVGEGI AKQLEARIAF RRAQKQAIQR AMRAGAKGIK TQVSGRLNGA D IARAEGYS EGTVPLHTLR ADIDYAWEEA DTTYGKLGVK VWIYRGEVLP TKKSVKGEK

Macromolecule #4: 30S ribosomal protein S4

• Macromolecule: Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 23.232605 KDa

Sequence

String:

MSRYTGPSWK QSRRYGISLT GSGKEIARRN YVPGQHGPNN RSKLSEYGLQ LAEKQKLRFT YGLSERQFRN LYVAATKVKE GTVGYNFMT LLEQRLDNVV YRLGLATTRR QARQFVNHGH ILVDGKRVDI PSFRVQPGQV ISVREKSMKV PAILEAVEAT K GRANFVSF DADKLEGTLV RLPERDEINP EINDALIVEF YNKMM

Macromolecule #5: 30S ribosomal protein S5

• Macromolecule: Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 17.609285 KDa

Sequence

String:

MAENRRNDRE QSEFEERVVS INRVTKVVKG GRRLRFAALV VVGDRNGRVG FGTGKAQEVP EAIRKAIEAA KKNLITVPMV GTTLPHEAL GVFGGGKILL KPAVEGAGVA AGGAVRAVLE LAGVADVTSK SLGSNTPINV VRATVDGLTQ LKRAEEVAAL R GKSVSDFA

Macromolecule #6: 30S ribosomal protein S6

• Macromolecule: Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 11.321831 KDa

Sequence

String:

MTKYEILYII RPNIDEEAKT ALVERFDAIL TENGAANLES KDWEKRKLAY EINDFREGIY HIATFEAETT SEALSEFDRL AKINLDILR HMIVKVEA

Macromolecule #7: 30S ribosomal protein S7

• Macromolecule: Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 17.713443 KDa

Sequence

String:

MRKNRAPKRE VLADPMYNSI VVTRLINRVM LDGKRGVAAQ IVYGAFKQIE EATGNNPLEV FETAMENIMP VLEVRARRVG GSNYQVPVE VRPERRTTLG LRWLVTIARN RGEHTMQDRL AKEILDAANN TGAAVKKRED THKMAEANRA FAHFRW

Macromolecule #8: 30S ribosomal protein S8

• Macromolecule: Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 14.692106 KDa

Sequence

String:

MVMTDPIADF LTRIRNGNMR KFDVVEAPAS KIKRQIAEIL KAEGYVKDVE YVEDNKQGVI RVFLKYGKNG EKVITNLKRI SKPGLRVYV KSDDVPKVLN GLGTAIISTS TGVVTDKVAR QTNVGGEVIA YIW

Macromolecule #9: 30S ribosomal protein S9

• Macromolecule: Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 14.124289 KDa

Sequence

String:

MAQVQYAGTG RRKNAVARVR LVPGTGKITV NGREVESYIP HADMRLVINQ PFAATQTEGS YDTLVNVNGG GVSGQAGAIR HGIARALLQ VDPDFRSALK RAGLLTRDAR MVERKKPGLK KARKASQFSK R

Macromolecule #10: 30S ribosomal protein S10

• Macromolecule: Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 11.764765 KDa

Sequence

String:

MATKKIRIRL KAYEHRILDA AAEKIVETAK RTNAEVSGPI PLPTDRSVYT VIRATHKYKD SREQFEMRTH KRLIDIIEPT QKTVDSLMK LDLPSGVNIE IKL

Macromolecule #11: 30S ribosomal protein S11

• Macromolecule: Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 13.310352 KDa

Sequence

String:

MAKITRKRRV KKNIESGIVH IQSTFNNTIV MITDVHGNAL AWSSAGALGF KGSKKSTPFA AQMASEAAAK AAQEQGLKTV SVTVKGPGS GRESAIRALA AAGLNVTSIS DVTPVPHNGA RPPKRRRV

Macromolecule #12: 30S ribosomal protein S12

• Macromolecule: Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 15.157884 KDa

Sequence

String:

MPTINQLVRK PRRAQVTKSK SPAMNVGYNS RKKVQTKLAS PQKRGVATRV GTMTPKKPNS ALRKFARVRL SNLMEVTAYI PGIGHNLQE HSVVLLRGGR VKDLPGVRYH IVRGALDTAG VADRKQSRSK YGAKKPKA

Macromolecule #13: 30S ribosomal protein S13

• Macromolecule: Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 13.519672 KDa

Sequence

String:

MARFAGVDIP NEKRIVISLT YVFGVGLQTS KKVLAAAGVS EDIRTKDLTS DQEDAIRREL DGLKLEGDLR REVSLNIKRL MEIGSYRGM RHRRGLPTRG QNTKNNARTR KGPAKSIAGK KK

Macromolecule #14: 30S ribosomal protein S14 type Z

• Macromolecule: Name: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 7.151611 KDa

Sequence

String:

MAKKSMVVKN QRPAKFSTQA YTRCERCGRP HSVYRKFKLC RICLRELAYK GQLPGVKKAS W

Macromolecule #15: 30S ribosomal protein S15

• Macromolecule: Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 10.367041 KDa

Sequence

String:

MAISKEKKQE IIAQYARKEG DTGSPEVQIA VLTWEINHLN DHIKSHKKDH ATQRGLMKKI GHRRNLLGYL RGKDVQRYRE LIASLGLRR

Macromolecule #16: 30S ribosomal protein S16

• Macromolecule: Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 10.270938 KDa

Sequence

String:

MSVKIRLTRM GSKKKPFYRI NVADSRAPRD GKFIETVGTY NPLVTENQVT LKEERVLEWL SNGAQPSDTV RNLLSKAGVM KKFHESKLS K

Macromolecule #17: 30S ribosomal protein S17

• Macromolecule: Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 10.166831 KDa

Sequence

String:

MERKQRKVYQ GRVVSDKMDK TITVVVETKR NHPVYGKRIN YSKKYKAHDE NNSAKTGDIV RIMETRPLSK DKHFRLVEIV EEAVII

Macromolecule #18: 30S ribosomal protein S18

• Macromolecule: Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 9.392087 KDa

Sequence

String:

MAQQRRGGFK RRKKVDFIAA NKIEVVDYKD TELLKRFISE RGKILPRRVT GTSAKNQRKV VNAIKRARVM ALLPFVAEDQ N

Macromolecule #19: 30S ribosomal protein S19

• Macromolecule: Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 10.595238 KDa

Sequence

String:

MGRSLKKGPF VDEHLMKKVE AQTNAERKSV IKTWSRRSTI FPNFVGLTIA VYDGRKHVPV YVQEDMVGHK LGEFAPTRTY RGHAADDKK TRR

Macromolecule #20: 30S ribosomal protein S20

• Macromolecule: Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 8.38474 KDa

Sequence

String:

MANIKSAIKR AELNKIANER NAQQKSAMRT LIKKFEAAPS EELYRAASST IDKAASKGLI HANKASRDKA RLAAKLG

Macromolecule #21: 30S ribosomal protein S21

• Macromolecule: Name: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 7.041308 KDa

Sequence

String:

MSKTLVRKNE SLDDALRRFK RSVTKAGTLQ ELRKREHYEK PSVKRKRKSE AARKRKKY

Macromolecule #22: 50S ribosomal protein L28

• Macromolecule: Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 7.187417 KDa

Sequence

String:

MSKECYFTGR KTVSSNNRSH AMNQTKRVVK PNLQKVTILE NGELKTVWAS AKALKKLPAG VERV

Macromolecule #23: 50S ribosomal protein L29

• Macromolecule: Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 7.874178 KDa

Sequence

String:

MKLSETKSLL KDLRALSVEE LTTREAELKK ELFDLRFQAA AGRLENTAKL DEVKKTIARV KTVQAELNK

Macromolecule #24: 50S ribosomal protein L30

• Macromolecule: Name: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 6.202401 KDa

Sequence

String:

MAQIKITLVN SPIGRIPAQR KTVKALGLGK LNSSVVKEGS PAILGMVNSI SHLVKVEEA

Macromolecule #25: 50S ribosomal protein L31 type B

• Macromolecule: Name: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 9.366479 KDa

Sequence

String:

MKQNIHPNYQ PVVFMDTTTG YKFLTGSTKG SKETVEWEDG NTYPLIRVEI SSDSHPFYTG RQKFQAADGR IARFEKKYGK Q

Macromolecule #26: 50S ribosomal protein L32

• Macromolecule: Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 6.681631 KDa

Sequence

String:

MAVPARHTSS AKKNRRRTHY KLTAPTVTFD ETTGDYRHSH RVSLKGYYKG RKVRDTK

Macromolecule #27: 50S ribosomal protein L33 3

• Macromolecule: Name: 50S ribosomal protein L33 3 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 5.567652 KDa

Sequence

String:

MLRKAGLACT VCGSRNYTLN LSSVAKEKRV EVKKFCRTCG KHTLHKETR

Macromolecule #28: 50S ribosomal protein L34

• Macromolecule: Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 5.200206 KDa

Sequence

String:

MKRTYQPHKK SRKTTHGFRS RMATKNGRRV LAARRRKGRA SLTV

Macromolecule #29: 50S ribosomal protein L35

• Macromolecule: Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 7.844419 KDa

Sequence

String:

MPKQKTHRAS AKRFKRTGNG GLKRFRAYTS HRFHGKSVKQ RRQLRKASMV SKGDFKRIRR MVATMK

Macromolecule #30: 50S ribosomal protein L36

• Macromolecule: Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 4.432446 KDa

Sequence

String:

MKVRPSVKPI CEYCKVIRRN GRVMVICPAN PKHKQRQG

Macromolecule #33: 50S ribosomal protein L2

• Macromolecule: Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 29.641348 KDa

Sequence

String:

MGIKVYKPTT NGRRNMTGSD FAEITTSTPE KSLLVSMSKT AGRNNTGRIT VRHHGGGHKR KYRMIDFKRT TDNVVAKVAT IEYDPNRTA NIALIVYANG VKSYILAAKG LEVGMTVVSG PDADIKVGNA LPLANIPVGT LIHNIELKPG KGGQLVRSAG A SAQVLGSE GKYTLVRLQS GEVRMILSTC RATIGVVGNE QQSLINLGKA GRTRHMGIRP TVRGSVMNPN DHPHGGGEGR QP VGRKSPM TPWGKPALGL KTRNKKAKSS KLIVRRIND

Macromolecule #34: 50S ribosomal protein L3

• Macromolecule: Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 21.956568 KDa

Sequence

String:

MSKGILGKKV GMTQIFTDNG ELIPVTVIEA TPNTVLQVKS VETDGYEATQ VGFDTLREVL TNKPAKGHAA KANTTPKRFV REFKGLEGA EVGAEITVDT FAAGDVVDVT GTSKGKGFQG PIKRHGQSRG PMAHGSRYHR RPGSMGPVAA NKVPKGKKLA G RMGNKRVT VQNLVIAQVL PEKNVILVKG NVPGAKKSLI VVKSAIKAK

Macromolecule #35: 50S ribosomal protein L4

• Macromolecule: Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 22.393461 KDa

Sequence

String:

MAKVSLFKQD GSQAGEVTLN DSVFGIEPNE SVVFDVVISQ RASLRQGTHA HKNRSAVSGG GKKPWRQKGT GRARQGSTRS PQWRGGGTV FGPNPRSYAY KLPQKVRQLA LKSVYSTKVT DGKLIAVDTL DFAAPKTAEF AKVISALSIE RKVLVVLPNE G NEFAELSA RNLENVKVTT ANSASVLDIV SADKLLVVQS ALTQIEEVLA

Macromolecule #36: 50S ribosomal protein L5

• Macromolecule: Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 20.03427 KDa

Sequence

String:

MTNRLKEKYT NEVVPALTEQ FNYTSIMAVP KVDKIVINMG VGDAVNNSKN LDKAVAELAL ISGQKPLITK AKKSVAAFRL REGMPIGAK VTLRGERMFE FLDKLVTVSL PRVRDFHGVS NKAFDGRGNY TLGVKEQLIF PEINYDDVDK VRGMDIVIVT T ANTDEESR ELLAKLGMPF AK

Macromolecule #37: 50S ribosomal protein L6

• Macromolecule: Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 19.313266 KDa

Sequence

String:

MSRIGNKVIV IPAGVSVEVN GATVTVKGPK GELVRSFNEN ITLEIAENEI TVKRPNDTKE MKMLHGTTRA LLANMVEGVS NGFSKALEM IGVGYRAQLQ GTKLVLSVGK SHQDEVEAPE NIKFVVATPT SIVVEGISKE AVGQTAAYIR SRRSPEPYKG K GIRYVGEY VRRKEGKTGK

Macromolecule #38: 50S ribosomal protein L13

• Macromolecule: Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 16.192855 KDa

Sequence

String:

MMKTTFMANA QNVDRKWYVV DATDVPLGRL SAVVASVLRG KNKPTYTPHA DTGDFVIVVN AEKIKLTGKK ATDKVYYSHS LHPGGLKSV TAGELREKNA VRLIEKSVKG MLPHNTLGRA QGMKLKVFVG AEHTHTAQKP EVLDITGLI

Macromolecule #39: 50S ribosomal protein L14

• Macromolecule: Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 12.944098 KDa

Sequence

String:

MIQTESRLKV ADNSGAKELL TIRVLGGSSR KFAGIGDIVV ATVKSAAPGG AVKKGEVVKA VIVRTKSGAK RPDGSYIKFD ENAAVLIRD DKTPRGTRIF GPVARELREG GYMKIVSLAP EVL

Macromolecule #40: 50S ribosomal protein L15

• Macromolecule: Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 15.376553 KDa

Sequence

String:

MELHSLKAAE GSRKVRNRVG RGTSSGNGKT SGRGQKGQKS RSGGGVRPGF EGGQTELFRR MPKRGFLNVN RKEYAIVNLE TLNRLEDGA TVSAETLVAA KIVKDVKSGV KVLANGELTA KNLTVKVAKV SAAAKAAIEA AGGSVEEA

Macromolecule #41: 50S ribosomal protein L16

• Macromolecule: Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 15.375058 KDa

Sequence

String:

MLVPKRVKHR REFRGKMRGY AKGGDTVSFG EYGLQATTSH WITNRQIEAA RIAMTRYMKR NGQVWIKIFP HKSYTAKAIG VRMGSGKGA PEGWVAPVKR GVVMFELGGV DEATAREALR LASHKLPVKT KFVKRGEA

Macromolecule #42: 50S ribosomal protein L17

• Macromolecule: Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 14.280492 KDa

Sequence

String:

MSNRKLGRTS SQRKAMLRDL TTDLIINETI VTTEARAKEV RRTVEKMITL GKKGDLSARR AAAAYVRNEI AIKDFNEETE TFPTALQKL FNDLAKRYEG RNGGYTRILK VEPRRGDAAP MAIIELV

Macromolecule #43: 50S ribosomal protein L18

• Macromolecule: Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 12.426203 KDa

Sequence

String:

MISKPDKNKL RQKRHTRVRG KISGTTETPR LNVFRSNTNI YAQVIDDVTG TTLASASSLK LTGTKTEQAA EVGKLVAEAA KAKGVEEVV FDRGGYLYHG RVAALATAAR EAGLKF

Macromolecule #44: 50S ribosomal protein L19

• Macromolecule: Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 13.129329 KDa

Sequence

String:

MNLIESINAA QLRTDIPDFR PGDTVRVHAK VVEGTRERIQ IFEGVVIARK NSGINETYTV RKISNGVGVE RIFPVHTPRV EKIEVIRHG KVRRAKLYYL RALTGKKARI AERRR

Macromolecule #45: 50S ribosomal protein L20

• Macromolecule: Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 13.726362 KDa

Sequence

String:

MARVKGSVAT RKRRKRILKL AKGYYGAKHR LFKTAKEQVM NSYYYAFRDR RQKKRDFRKL WIARINAAAR MNGLSYSKLM HGLKLADIE VNRKMLADIA IADAAAFTAL AEEAKKALAK

Macromolecule #46: 50S ribosomal protein L21

• Macromolecule: Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 11.444199 KDa

Sequence

String:

MSNYAIIKTG GKQVKVEEGS VIYVEKLNVE AGQTVTFDEV IFVGGETTKV GAPLVEGATV VGEVEKHGKQ KKVVTFQYKP KKHSHRKQG HRQPYTKVVI KSVNA

Macromolecule #47: 50S ribosomal protein L22

• Macromolecule: Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 12.49054 KDa

Sequence

String:

MAEITSAKAT AKTVRVSPRK TRLVIDLIRG KRVADAIAIL KFTPTKAAVE VENVLNSAIA NAENNFGLEK ANLVVSETFI NEGPTMKRF RPRAKGSASP INKRTAHITV VVAEKE

Macromolecule #48: 50S ribosomal protein L23

• Macromolecule: Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 10.819567 KDa

Sequence

String:

MSLYDIIRKP IITEASMAAM DQKKYTFEVD SRAHKLLIKQ AVEAVFDVKV ASVNTISVKP KSKRVGKYTG TKSGYKKAIV TLTEDSKTI EIFGEDAE

Macromolecule #49: 50S ribosomal protein L24

• Macromolecule: Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 10.897782 KDa

Sequence

String:

MFVKTGDTVK VIAGKDRGTT GKVIKALPKV NKVVVEGVAI MKKHQKPNSE NPSGAILEIE APIHVSNVQV LDKNGVAGRV GYKVVDDKK VRFNKKSGEI LD

Macromolecule #50: 50S ribosomal protein L27

• Macromolecule: Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 10.053518 KDa

Sequence

String:

MLELNLQLFA HKKGGGSTSN GRDSQAKRLG AKASDGELVS GGSILFRQRG THIHPGTNVG RGGDDTLFAK IEGTVKFEMK RGKKHVSVY PVVAK

Macromolecule #51: Ribosome hibernation promotion factor

• Macromolecule: Name: Ribosome hibernation promotion factor / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria); Strain: MG1363
• Molecular weight: Theoretical: 21.345988 KDa

Sequence

String:

MIKFNIRGEN VEVTDAIRAY VEDKIGKLDK YFNDGHEVTA YVNLKVYTEK RAKVEVTLPA KNVTLRAEDT SQDMYSSIDF VEEKLERQI RKYKTRMNRK PRNAVPTGQV FGDEFAPLDT TDEVAEDHVD IVRTKHVALK PMDAEEAVLQ MDMLGHDFYV F TDADSNGT HVVYRRTDGR YGLIETE

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 291.15 K / Instrument: FEI VITROBOT MARK III

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Specialist optics: Spherical aberration corrector: Cs-corrector (CEOS, Heidelberg, Germany)
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 25.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: CTFFIND (ver. 4)
• Startup model: Type of model: OTHER / Details: RANSAC in Xmipp
• Initial angle assignment: Type: RANDOM ASSIGNMENT / Software - Name: Xmipp
• Final 3D classification: Software - Name: RELION
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION
• Final reconstruction: Number classes used: 2 / Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 5.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 43530

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: FLEXIBLE FIT

Output model

PDB-5myj:
Structure of 70S ribosome from Lactococcus lactis