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CryoEM map of MsbA in POPG nanodisc

by single particle reconstruction, at 5 A resolution

Entry
Summary
Database / IDEM DATA BANK (EMDB) / 8669
TitleCryoEM map of MsbA in POPG nanodisc
Mapmap of MsbA with LPS in POPG nanodisc, filtered to 5A and sharpened with -280 B-factor
SampleMsbA with LPS in POPG nanodisc
AuthorsMi W, Walz T, Liao M
DateDeposition: 2017-04-11, Header release: 2017-07-19, Last update: 2017-07-19
Map data statusOn hold (released when the article is published)
EMDB SitesEMDB @PDBe (EU), EMDB @RCSB (USA)
Related Structure Data
Related Entries

Cite: data citing same article

Article
Citation - Primary
ArticleNature, Year 2017
TitleStructural basis of MsbA-mediated lipopolysaccharide transport.
AuthorsWei Mi, Yanyan Li, Sung Hwan Yoon, Robert K Ernst, Thomas Walz, Maofu Liao
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, Maryland 21201, USA.
Laboratory of Molecular Electron Microscopy, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA.
LinksPubMed: 28869968, DOI: 10.1038/nature23649
Map
FileOn hold (released when the article is published)
Density
Contour Level:0.034 (by author)
Minimum - Maximum: -0.06834029 - 0.14602281
Average (Standard dev.): 3.7362337E-5 (0.00919677)
Data TypeImage stored as Reals
Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions192192192
Origin-96-96-96
Limit959595
Spacing192192192
Unit CellA= B= C: 245.76 A
Alpha=beta=gamma: 90 degrees
Pixel SpacingX= Y= Z: 1.28 A
Annotation Detailsmap of MsbA with LPS in POPG nanodisc, filtered to 5A and sharpened with -280 B-factor
Supplement
Sample
NameMsbA with LPS in POPG nanodisc
Number of Components2
Theoretical Mass0.0672MDa
Component #1: protein - MsbA with LPS in POPG nanodisc
Scientific nameMsbA with LPS in POPG nanodisc
Theoretical Mass0.0672 MDa
Scientific Name of SpeciesEscherichia coli

NCBI taxonomy562
Recombinant expressionNo
Engineered SourceNCBI taxonomy: 866768
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Component #2: protein - MsbA
Scientific nameMsbA
Recombinant expressionNo
Engineered SourceNCBI taxonomy: 562
Expression system: Escherichia coli
Experiment
Sample Preparation
Specimen Stateparticle
Specimen Conc1 mg/ml
BufferpH: 7.5
Vitrification
Cryogen NameETHANE
Imaging
MicroscopeFEI POLARA 300
Electron Gun
Electron SourceFIELD EMISSION GUN
Accelerating Voltage300 kV
Electron Dose47 e/A**2
Illumination ModeFLOOD BEAM
Lens
Imaging ModeBRIGHT FIELD
Specimen Holder
ModelOTHER
Camera
DetectorGATAN K2 (4k x 4k)
Processing
Methodsingle particle reconstruction
3D reconstruction
Resolution By Author5 A
Resolution MethodFSC 0.143 CUT-OFF
Single Particle
Number of Projections55199
Download
Data from EMDB
Header (meta data in XML format)emd-8669-v19.xml (5.4 KB)
emd-8669-v20.xml (11.1 KB)
emd-8669.xml (5.4 KB)
FTP directoryftp://ftp.pdbj.org/pub/emdb/structures/EMD-8669