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- EMDB-8576: Structure of the Plasmodium falciparum 80S ribosome bound to the ... -

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Basic information

Entry
Database: EMDB / ID: EMD-8576
TitleStructure of the Plasmodium falciparum 80S ribosome bound to the antimalarial drug mefloquine
Map data
Sample
  • Complex: Plasmodium falciparum 80S ribosome bound to mefloquine
    • RNA: x 3 types
    • Protein or peptide: x 42 types
  • Ligand: x 3 types
Function / homology
Function and homology information


Translesion synthesis by REV1 / Translesion Synthesis by POLH / : / Translesion synthesis by POLK / Translesion synthesis by POLI / Josephin domain DUBs / : / Metalloprotease DUBs / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / ER Quality Control Compartment (ERQC) ...Translesion synthesis by REV1 / Translesion Synthesis by POLH / : / Translesion synthesis by POLK / Translesion synthesis by POLI / Josephin domain DUBs / : / Metalloprotease DUBs / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / ER Quality Control Compartment (ERQC) / Iron uptake and transport / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Formation of a pool of free 40S subunits / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Aggrephagy / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / : / Orc1 removal from chromatin / CDK-mediated phosphorylation and removal of Cdc6 / KEAP1-NFE2L2 pathway / UCH proteinases / Ub-specific processing proteases / Neddylation / Antigen processing: Ubiquitination & Proteasome degradation / protein-RNA complex assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / protein tag activity / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / ribosome biogenesis / cytoplasmic translation / 5S rRNA binding / cytosolic large ribosomal subunit / ubiquitin-dependent protein catabolic process / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / ubiquitin protein ligase binding / RNA binding / metal ion binding / nucleus / cytoplasm
Similarity search - Function
Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L29e / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal L29e protein family / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain ...Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L29e / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal L29e protein family / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 60S ribosomal protein L35 / Ribosomal protein L30e, conserved site / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal L27e protein family / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L34e / Ribosomal protein L31e, conserved site / Ribosomal protein L37ae / Ribosomal protein L14e domain / 60S ribosomal protein L6E / Ribosomal protein L19, eukaryotic / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal L40e family / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L44e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L7A/L8 / Ribosomal protein L10e signature. / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e / Ribosomal protein L14 / Ribosomal protein L30, N-terminal / Ribosomal protein L14, KOW motif / Ribosomal L30 N-terminal domain / Ribosomal protein L14 / Ribosomal L37ae protein family / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L39e, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal_L31e / Ribosomal protein L15e, conserved site / Ribosomal protein L30e signature 1. / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / 60S ribosomal protein L19 / Ribosomal protein L32e, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L36e signature. / Ribosomal protein L37e / Ribosomal protein L39e signature. / Ribosomal protein L21e / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L31e / Ribosomal protein L37ae/L37e / Ribosomal protein L24e-related / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L39e / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L39e domain superfamily
Similarity search - Domain/homology
Ribosomal protein L15 / 60S ribosomal protein L39 / Ribosomal protein L37 / 60S ribosomal protein L18-2, putative / 60S ribosomal protein L19 / 60S ribosomal protein L27a, putative / 60S ribosomal protein L41 / 60S ribosomal protein L29 / 60S ribosomal protein L26, putative / Large ribosomal subunit protein eL43 ...Ribosomal protein L15 / 60S ribosomal protein L39 / Ribosomal protein L37 / 60S ribosomal protein L18-2, putative / 60S ribosomal protein L19 / 60S ribosomal protein L27a, putative / 60S ribosomal protein L41 / 60S ribosomal protein L29 / 60S ribosomal protein L26, putative / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL42 / 60S ribosomal protein L7, putative / 60S ribosomal protein L32 / 60S ribosomal protein L2 / 60S ribosomal protein L4 / 60S ribosomal protein L31 / 60S ribosomal protein L36 / 60S ribosomal protein L13 / Large ribosomal subunit protein eL22 / 60S ribosomal protein L11a, putative / 60S ribosomal protein L34 / Ubiquitin-60S ribosomal protein L40 / 60S ribosomal protein L17, putative / 60S ribosomal protein L18a / 60S ribosomal protein L6-2, putative / 60S ribosomal protein L23, putative / 60S ribosomal protein L23 / 60S ribosomal protein L6, putative / 60S ribosomal protein L24, putative / 60S ribosomal protein L35ae, putative / 60S ribosomal protein L28 / 60S ribosomal protein L38 / 60S ribosomal protein L35, putative / 60S ribosomal protein L3 / 60S ribosomal protein L30e, putative / 60S ribosomal protein L13, putative / 60S ribosomal protein L27 / 60S ribosomal protein L14, putative / 60S ribosomal protein L21 / 60S ribosomal protein L7a / 60S ribosomal protein L5, putative / 60S ribosomal protein L10, putative
Similarity search - Component
Biological speciesPlasmodium falciparum 3D7 (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsWong W / Bai X-C / Brown A / Scheres S / Baum J
Funding support Australia, United Kingdom, 8 items
OrganizationGrant numberCountry
NHMRCAPP1024678 Australia
Medical Research Council (United Kingdom)MC_UP_A025_1013 United Kingdom
NHMRCAPP1053801 Australia
Wellcome TrustWT096570 United Kingdom
Medical Research Council (United Kingdom)MC_U105184332 United Kingdom
Human Frontier Science ProgramRGY0071/2011 United Kingdom
Wellcome Trust100993/Z/13/Z United Kingdom
Department of Industry, Innovation, Science, Research and Tertiary Education, Australian Government|Australian Research CouncilFT100100112 Australia
CitationJournal: Nat Microbiol / Year: 2017
Title: Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis.
Authors: Wilson Wong / Xiao-Chen Bai / Brad E Sleebs / Tony Triglia / Alan Brown / Jennifer K Thompson / Katherine E Jackson / Eric Hanssen / Danushka S Marapana / Israel S Fernandez / Stuart A Ralph ...Authors: Wilson Wong / Xiao-Chen Bai / Brad E Sleebs / Tony Triglia / Alan Brown / Jennifer K Thompson / Katherine E Jackson / Eric Hanssen / Danushka S Marapana / Israel S Fernandez / Stuart A Ralph / Alan F Cowman / Sjors H W Scheres / Jake Baum /
Abstract: Malaria control is heavily dependent on chemotherapeutic agents for disease prevention and drug treatment. Defining the mechanism of action for licensed drugs, for which no target is characterized, ...Malaria control is heavily dependent on chemotherapeutic agents for disease prevention and drug treatment. Defining the mechanism of action for licensed drugs, for which no target is characterized, is critical to the development of their second-generation derivatives to improve drug potency towards inhibition of their molecular targets. Mefloquine is a widely used antimalarial without a known mode of action. Here, we demonstrate that mefloquine is a protein synthesis inhibitor. We solved a 3.2 Å cryo-electron microscopy structure of the Plasmodium falciparum 80S ribosome with the (+)-mefloquine enantiomer bound to the ribosome GTPase-associated centre. Mutagenesis of mefloquine-binding residues generates parasites with increased resistance, confirming the parasite-killing mechanism. Furthermore, structure-guided derivatives with an altered piperidine group, predicted to improve binding, show enhanced parasiticidal effect. These data reveal one possible mode of action for mefloquine and demonstrate the vast potential of cryo-electron microscopy to guide the development of mefloquine derivatives to inhibit parasite protein synthesis.
History
DepositionJan 27, 2017-
Header (metadata) releaseMar 1, 2017-
Map releaseMar 1, 2017-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.12
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.12
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5umd
  • Surface level: 0.12
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8576.png

Downloads & links

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Map

FileDownload / File: emd_8576.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.03 Å
Density
Contour LevelBy AUTHOR: 0.12 / Movie #1: 0.12
Minimum - Maximum-0.5065006 - 0.8825569
Average (Standard dev.)0.0001856431 (±0.033729594)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 603.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.031.031.03
M x/y/z450450450
origin x/y/z0.0000.0000.000
length x/y/z463.500463.500463.500
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS450450450
D min/max/mean-0.5070.8830.000

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Supplemental data

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Half map: #1

Fileemd_8576_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_8576_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Plasmodium falciparum 80S ribosome bound to mefloquine

EntireName: Plasmodium falciparum 80S ribosome bound to mefloquine
Components
  • Complex: Plasmodium falciparum 80S ribosome bound to mefloquine
    • RNA: 28S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • Protein or peptide: 60S ribosomal protein L2
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: 60S ribosomal protein L11a, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L6, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L6-2, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L7-3, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L13, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L23, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L14, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L27a, putativeRibosome
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L10, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L5, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L18-2, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21
    • Protein or peptide: 60S ribosomal protein L17, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L22, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L23
    • Protein or peptide: 60S ribosomal protein L26, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L24, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S ribosomal protein L35, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L29, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L7, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L30e, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L35Ae, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: Ubiquitin-60S ribosomal protein L40
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: 60S ribosomal protein L37a
    • Protein or peptide: 60S ribosomal protein L44
  • Ligand: Mefloquine
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Plasmodium falciparum 80S ribosome bound to mefloquine

SupramoleculeName: Plasmodium falciparum 80S ribosome bound to mefloquine
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#45
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote) / Location in cell: cytoplasm
Molecular weightTheoretical: 4.2 MDa

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Macromolecule #1: 28S ribosomal RNA

MacromoleculeName: 28S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 1.216212125 MDa
SequenceString: GUUUGAUUUG AUGUUAGGAG GGCAAAUCCG CCGAAUUUAA GCAUAUAAUU AAGCGGAAGA AAAGAAAAUA ACUAUGAUUC CUUUAGUAA CGGCGAGUGA ACAAGGACCA GCUCAAACGG UUAAUCCUUC GUUUGGUGCA AACCUUUCGA CGGAAUUGUC G UUGAAUUA ...String:
GUUUGAUUUG AUGUUAGGAG GGCAAAUCCG CCGAAUUUAA GCAUAUAAUU AAGCGGAAGA AAAGAAAAUA ACUAUGAUUC CUUUAGUAA CGGCGAGUGA ACAAGGACCA GCUCAAACGG UUAAUCCUUC GUUUGGUGCA AACCUUUCGA CGGAAUUGUC G UUGAAUUA UUCCAUGAAU GCCUUUAAUU UGAGCAAAGU GAGCAGGAAU GCUUCCCCAU AGAGGGUGAA AGGCCCAUAG UU CUUUUUA AUUUAUUCAU UGGAAUUUUU CAUGUCGAGU CGUGUUCUUU GAGAUUGGAG CACUAAUACG UGUGAUACAU UUC ACAUAA AGCUAAAUAU AUGUAGGAGA CCGAUAGCAA ACAAGUACCG UGAGGGAAAG AUGAAAUAGU ACUCAGGAAU GAGC AAUUA AAUAGUACCU GAAAUCGUUA AGAUGGAACG GAUUAAGAGA GAAAACAAGU AAAGAGGGGA AUUUUUAAUU UUUUU UGUU AUAAUUCUCU UCUUUAUUAA AAGAAACAUC AGUGAUUAAU UUAAUUUCAA UAAAGCAAUC CCCUGAAAUU CAAAAU UUC UUUUAAUUUU GUUUUCACUU UCUCCCCGCA CUAAUGUGGG GAAAACUGGC UUUAUUUCUU CAAUUAUUUU UUUUGCU GA GGAAUUUUAA AAUUAUUUGA AUUUUUCUUC UUAUAAUUUA AGUUGUUUCU AUACAGUACU UUCUUAACCC ACUCGUCU U GAAACACGGA CCAAGGAGUC UAGCAAAUGU GCAAGUGUAU AUGAUUCUUU AAACAUUUCU CUUUUUAAUA UACGCAUAA UUAAUGUAAU AUGUUUUCUU CAUUGUAGAU UUGUGGUGUU UAAUUUUUAU UAAAUCCCCA CUUUGCAUAC AAUACCGGUA AGCAAUUAU GCUUUAUUGA GUACGAGCAU AUUUGGUAGG ACCCGAGAGG CUUUGAACUA AGCGUGAUGA GAUUGAAGUC A GACGAAAG UCUGAUGGAG GAUCGAGUUG AUACUGACGU GCAAAUCGUU CAUUUCAAUC ACGUUUAGGG GCGAAAGACU AA UCGAAAA GCCUAUUAGC UGGUUAUUUU CGAAAGAUCU CUCAGGAUCG CUGGAGUUGA UUAAUAUAAU UUUACCAGGU AGA GAUAAU GAUUAGAGGA AUCAGGGAAA UCGUUUUCCU UGACCUAUUC UCAAACUCCC AAUAGGUAAA AAGGGCGAAA UAAC UCAAU UUGUAUUUCA CUCAUAUUAA AUAAAAUAAC UCCAAGUGGG CCAUUUUUGG UAAGCAGAAC UGGCGAUGAG GGAUG CUCC UAACGCUUGG AUAAGGUGCC UAAAUAUUCG CUCAUGAGAU CCCAUAAAAG GUGUUGGUUC AUAAUGACAG UAGGAC GGU GGUCAUGGAA GUCGAAAUCC GCUAAGGAGU GUGUAACAAC UCACCUACCG AAUGAACUAG CCCUGAAAAU GGAUGGC GC UAAAGCGGAU UACCGAUACC AAGCCAUAAA AAGAACAGAA AAAAUUUAUU UUUUUUUUAG AAUCUUUUUA UGAGUAGA A AAUCGUGGGG UUUGUGUUGA AGCGAAAUAC GUGAGUUUUC GUGGAACAUC UCCCUAGUGC AGAUCUUGGU GGAAGUAGC AACUAUUCAA AUGAGAACUU UGAAGACUGA AGUGGAGAAG GGUUUCUUGU CAACUGUGAU UGAACAAGAG UUAGCCGCUC CUAAGGGAU AGCUGAAAAG UGUUUAAAAG GGGGUUCCUU CCCCGUCUCA AAAGGGAAAC AGGUUGAUAU UCCUGUGCCA A UAGUAUUA UGAGUUUCUU AGAUGGUAAC AUAUAUAUAA AUGAACUCCU UUACAUAGGC UUUACACUCG GGGUGCGUUU UC UUUGCAC UUUACCUUUA UAACAAACCU UGGAAUCAAU UUACUUGGAG AAGAGGUUCG UUGAACUCAA UUCAAAAAAU UUC UUGAAC GGGGUUUUCG GAUUCAGUUC AUUUUAUUUU UUGUUUUGUA GCAAUAGUAA UUCGUUUUUA UGAAUUAUCC GAAG UGGUA AGGACUAUCC UUGAAAAAAG GAGGGAACGG CUUUGUAACU UGGUUUCUUC UGAUUCCAUU UUGCUUUGCU UAUAC UCUU AAUACUUGUA UGAGCGUACC AACAACCGCA UCAGGUCUCC AAGGUUAACA GCCUCUGGUU AAAUAGAAAA AAGUAG GUA AGGGAAGUCG GCAAAAUAGA UCCGUAACUU CGGGAAAAGG AUUGGCUCUG AGGACAUUAG AAAAGAGAAG AAAAAAA GA GGGUCGAAAA UAAAAUUGCA GUCUUUAUUU GCUUUUCUUU CGAUUUGCUU GUAAUCUGCU UUUUCUUUUU CUUCUUUC U UUUUUUCUCC CCCUCUUUUC GCCUUCACUU UAUUGUAAUU UUAUUACUUU AAUUUGAUAC CUAUAAUGUU AACUCAGAA CUGAAACGGA CAAGGGGAAU CCGACUGUUU AAUUAAAACA UAGCAUUGUG AAAAGCCACA ACUGGUAUUA ACACAAUGUG AUUUCUGCC CAGUGCUUUG AAUGUCAACU UGAUGAAAUU CAAUCAAGCG CAGGUAAACG GCGGGAGUAA CUAUGACUCU C UUAAGGUA GCCAAAUGCC UCGUCAUCUA AUUAGUGACG CGCAUGAAUG GAUUAACGAG AUUCCCACUG UCCCUACUUG CU AUCUAGC GAAACCACAG CCAAGGGAAC GGGCUUGGCA AAAUCAGCGG GGAAAGAAGA CCCUGUUGAG CUUUACUCUA GUC UGGCUU UGUGAAACGA CUUAAGAGGU GUAGCAUAAG UGGGAGUAGA AACUGAAAUA UGUUUUUACG ACAGUGAAAU ACCA CUACU UUUAAAGUUG UUUUACUUAU CCAUUUAUAG GGAAAUAUAU UAUGCUUUAU CCUUCGGGAA GGCAUUCUGA UAUUA UUUG AAUUUUUAAG AAACUUGUUU CUUAUCUUUC UCCCAAUUUC UAUGGAGACA UAGCCAGGUG GGGAGUUUGA CUGGGG CGG UACAUCUGUU AAAUAUUAAC GCAGAUGUCC AAAGACAAGC UCAAAGAGAA CAGAAAUCUC UUGUAGACUA AAAGGGG AA AAGCUUGUUU GAUUUCUAUU UUCAGAACAA GUAGAAAACG UGAAAGCGUG GCCUAUCGAU CCUUUAUAUU UGCAAAAU G ACGUAAAUUA CUUACUACUG UGCAUAUAGA GGUGUCUGAA AAGUUACCAC AGGGAUAACU GGCUUGUGGC UGCCAAGCG CUCCUAGCGA CGUAGCUUUU UGAUCCUUCG AUGUCGGCUC UUCCUAUCAU UGGGACGCAG AAGUCUCAAA GUGUCGGAUU GUUCACCCG CUAAUAGGGA ACGUGAGCUG GGUUUAGACC GUCGUGAGAC AGGUUAGUUU UACCCUACUG AUGAAUUUUA U UAUAUUUU UUAUAUAUAC AUAUAGUAUU GUGACAGUAA UCCAACUUGG UACGAGAGGA UUAGUUGGUU CAGACAAUUG GU ACAGCAA UUGGUUGACA AACCAGUGUU GCGAAGCUAA GUCUGUUGGA UAAUGGCUGA ACGCCUCUUA AGCCAGAAAC CAU GCUGAU UAAACAAUAC UAUUUCGUUC UUUUUUGUUU CCUUUUUUAC UGCUAAUGUA AAAGAGAAAU UAUAUAUGUA CCUU UUUUU GGUACAUAAA UAAUCCUAUC ACCUUUUUCG UGAGCUGUGA GCUGUGAGCU AGGCAAUAGU UCUUUAACAA CAAAA AAGA AUUGCACAAA CUGUAGACGA CUUUUUUGCC UCAGGGUGCU GUAAACAUGA AAGUAAACUU UGUUUUACGA UCUGUU GAG GCUUAUCCCU AGUGGUAAAG UGUUU

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Macromolecule #2: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 38.410859 KDa
SequenceString:
UGACUCGUUC AUACUACAGU GGAUACACCA GAUCCCAUCA GAACUCUGAA GUUAAGCACU GUAAGGCUUG GCUAGUACUG AGGUGGGAG ACCGCUCGGG AACACCAGGU GAUGAGUCAG

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Macromolecule #3: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 51.206461 KDa
SequenceString:
UAGCACAGUC UUAACGAUGG AUGUCUUGGU UCCUACAGCG AUGAAGGCCG CAGCAAAAUG CGAUACGCAA UGAAAAUUGC AGUGACUGU GAAUCAUCAG AAUGCUGAAU GUAAACUACA CCAAUUCCCU UCGGGGAAUA GUGGUACUCC UACAGAAAAU

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Macromolecule #4: 60S ribosomal protein L2

MacromoleculeName: 60S ribosomal protein L2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 28.064998 KDa
SequenceString: MGRVIRGQRK GRGSIFKSHN HHRKGAAKLR HLDYCEKKGY IKGLVKDIIH DPGRGAPLAK VIFKRTEKYG KKEELIIASE GMFTGQYIS CGTKAPLSVG NILPIGKMPE GTLICNLEHR TGNRGTLVKA SGCYATVVGQ SEDGKKTKVR LPSGAKKTID A KARAMVGV ...String:
MGRVIRGQRK GRGSIFKSHN HHRKGAAKLR HLDYCEKKGY IKGLVKDIIH DPGRGAPLAK VIFKRTEKYG KKEELIIASE GMFTGQYIS CGTKAPLSVG NILPIGKMPE GTLICNLEHR TGNRGTLVKA SGCYATVVGQ SEDGKKTKVR LPSGAKKTID A KARAMVGV VGAGGRIDKP ILKAGVAHHK YRVKRNCWPK VRGVAMNPVE HPHGGGNHQH IGHPSTVSRS APAGQKVGLI AA RRTGLLR GAKKTKLVGK SEE

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Macromolecule #5: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 44.31818 KDa
SequenceString: MSHRKFERPR HGSLGFLPRK RCKRLRGKIR SFPKDDKEKP PHFTAFMGYK AGMTHIVREV DKPGSKLHKK EIVEACTIIE CAPMVVVGI VGYRETPKGL RILSTVWANH VSDEFRRRYY KNWYKSEKKA FTKSLNVPEA TKNCLLKRIE KYCTVLRAIC H TQPSKTPL ...String:
MSHRKFERPR HGSLGFLPRK RCKRLRGKIR SFPKDDKEKP PHFTAFMGYK AGMTHIVREV DKPGSKLHKK EIVEACTIIE CAPMVVVGI VGYRETPKGL RILSTVWANH VSDEFRRRYY KNWYKSEKKA FTKSLNVPEA TKNCLLKRIE KYCTVLRAIC H TQPSKTPL RMKKAHIMEV QINGGSMKEK LEFVKEMLEK NLPVSTVFNP NEMIDVISVT KGHGTKGVVS RYGVKRLPRK TH RGLRKVA CIGAWHPARV QFQIPRHGQK GYFHRTERNK KIYRIGLKTD KNSASTDADI TEKKITPMGG FPHYGVVNED FLL LKGCVA GTKKRPITLR KNIVPQVSRD ALAQVSLKFI DTSSKIGHGR FQTSEEKVKY YGPLKKDLKA

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Macromolecule #6: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 46.311688 KDa
SequenceString: MATIRPVANV YSTNGKNVVG EVEIPVVFQT PIRNDLIQSV YTNMSKNRRH PYAVKLGAGY ETSAESWGTG RAVARIPRVP GGGTHRAGQ GAFGNMCRGG GMFNPTKIWR RWGRKVNLKE KRYAVCSSIA ASGVTSLVLA RGHRISHLKE VPLVVSNDIE S LSKTKEAV ...String:
MATIRPVANV YSTNGKNVVG EVEIPVVFQT PIRNDLIQSV YTNMSKNRRH PYAVKLGAGY ETSAESWGTG RAVARIPRVP GGGTHRAGQ GAFGNMCRGG GMFNPTKIWR RWGRKVNLKE KRYAVCSSIA ASGVTSLVLA RGHRISHLKE VPLVVSNDIE S LSKTKEAV NFLVSLGLKD EVNRLVKSKK IRAGKGKMRN RKYKIRNGPL IIYENDNGVK KAFRNIPGVD LCKVTKLNLL KL APGGSIG RLCIWSESAF KKLDVIYGKI HEKKVTKKNY ILPKSIVHNP DIYRIIHSDK VQASLLAKKK PCKKRLQNKN SLT NFAVRC RLNPAYKLLR SLAVLRMRKS ILEKSKNKKE KRVQKQIQKK ELQKINHDYY KGVAKAVKKK KKREEKKAKS KKTA NQAVI NVAAEE

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Macromolecule #7: 60S ribosomal protein L11a, putative

MacromoleculeName: 60S ribosomal protein L11a, putative / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 20.268666 KDa
SequenceString:
MENKEQNVMR EIKVNKLVLN ICVGESGDRL TRAARVLEQL TEQKPIFGKC RFTIRSFGVR RNEKISCFVT VRGKKALEIL EKGLKVKEY ELRRKNFSDT GNFGFGIQEH IDLGIKYDPS TGIYGMDFYV HLSRSGYRVT RRTRRRSKIS KTHKVTKEDA M KWFQTKFD GILLK

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Macromolecule #8: 60S ribosomal protein L6, putative

MacromoleculeName: 60S ribosomal protein L6, putative / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 21.638562 KDa
SequenceString:
MKTIVSTQKV LIPEGVKVAI NARKVTVSGK YGTLRRSFRH LPIDIRLNKL KKYIKVVMWF GVPDSLACIR TVCTHLKNMF TGVTKKFLY KMRLVHAHFP INSNIVDNNT RIEIRNYLGE KSVRFVKALP GVVIEKSPNV KDEIYVSGAD IENVSLTAAL I HQSVLCRN KDIRKFLDGI YVSEVTTVEK DE

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Macromolecule #9: 60S ribosomal protein L6-2, putative

MacromoleculeName: 60S ribosomal protein L6-2, putative / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 25.587623 KDa
SequenceString: MTNTSNELKH YNVKGKKKVL VPVNAKKTIN KKYFGRKVAS KKKYVVQRKL RKSIEVGKVA IILTGKHMGK RCIITKILNS GLLAVVGPY EINGVPLKRV DSRYLVVTST NIFNFENIAK LKDDFLNYAQ DIDDDSFIKT LEIKKKQKKL LKNKNEALFM N NVIDKIKE ...String:
MTNTSNELKH YNVKGKKKVL VPVNAKKTIN KKYFGRKVAS KKKYVVQRKL RKSIEVGKVA IILTGKHMGK RCIITKILNS GLLAVVGPY EINGVPLKRV DSRYLVVTST NIFNFENIAK LKDDFLNYAQ DIDDDSFIKT LEIKKKQKKL LKNKNEALFM N NVIDKIKE IRKEDPKVQK LEGIQKDIGS LLKPEILKNK VFAHYLKSKF TLRNDMVLHK MKF

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Macromolecule #10: 60S ribosomal protein L7-3, putative

MacromoleculeName: 60S ribosomal protein L7-3, putative / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 32.760455 KDa
SequenceString: MAKDKKQQKK PSKKTPAPCP LSTKVTVKKE IKKEKKTGIK DNPLIFEKKK RSNIIGVGVR PKKDLSKYVK WPRYIRIQRK KKILLQRLK VPPSINQFNH TLPKSQTQDL LNFLKAYKPE SKTDKKQRLL NKAKEALNKN ITKDKKPLFL KYGINHITKL V ENKKANLV ...String:
MAKDKKQQKK PSKKTPAPCP LSTKVTVKKE IKKEKKTGIK DNPLIFEKKK RSNIIGVGVR PKKDLSKYVK WPRYIRIQRK KKILLQRLK VPPSINQFNH TLPKSQTQDL LNFLKAYKPE SKTDKKQRLL NKAKEALNKN ITKDKKPLFL KYGINHITKL V ENKKANLV VIANDVSPIE LVLFLPALCR LKEVPYCIVK DKATLGKLVH KKTATAVCLE SVKKEDQEKL DYFAKVCKEN FN DNVDLRR KWGGQKMSAK SMLLKKMKDK ARKIEEAKKK EISAKL

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Macromolecule #11: 60S ribosomal protein L13, putative

MacromoleculeName: 60S ribosomal protein L13, putative / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 23.805344 KDa
SequenceString: MYKKEYVIDC KGHLLGRLAS LIAKELLNGQ RIVAVRCEDI NISGSLYRNK LKYQEFLRLR TNTNPKKGPL HLREPSKILW RCVRGMLPH KTYKGQLALK KLKVCVGMPY PYDKKKKYVL PSALRAFRLK KHRRYCRLGT LSSRVGWNYD TLVKKNEVLR K QVSKAYYK ...String:
MYKKEYVIDC KGHLLGRLAS LIAKELLNGQ RIVAVRCEDI NISGSLYRNK LKYQEFLRLR TNTNPKKGPL HLREPSKILW RCVRGMLPH KTYKGQLALK KLKVCVGMPY PYDKKKKYVL PSALRAFRLK KHRRYCRLGT LSSRVGWNYD TLVKKNEVLR K QVSKAYYK KKVNNLNEKK EIKTEALNLI NPEQRQVLEN FGYA

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Macromolecule #12: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 25.454143 KDa
SequenceString: MVAHNNVLPN VHLHKWWQHR VRVNFSKNIK KKKRRLLREK RRKANGGTPI EKLHPIVHCP TQRYNFRTRL GKGFTFEELK GAGLTPRAA QTIGICYDKR RKNRSEESLT KNVERLLKYK NSLVMIPLKK NKAKKGIGGI PADADKNTIK EFRNKKPLLS I FKKEKNTK ...String:
MVAHNNVLPN VHLHKWWQHR VRVNFSKNIK KKKRRLLREK RRKANGGTPI EKLHPIVHCP TQRYNFRTRL GKGFTFEELK GAGLTPRAA QTIGICYDKR RKNRSEESLT KNVERLLKYK NSLVMIPLKK NKAKKGIGGI PADADKNTIK EFRNKKPLLS I FKKEKNTK PFYETIEVSK IDKEFLAYKT LRRAKLAERR KNCRQQKKDI KFKSKDN

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Macromolecule #13: 60S ribosomal protein L23, putative

MacromoleculeName: 60S ribosomal protein L23, putative / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 15.015713 KDa
SequenceString:
MKRGRAGTLK NKMRITLSLP VGALINCCDN SGGKNLYIIA VQGFGSCLNR LPAASLGDMV LATVKKGKPD LRKKVLNAII CRQSKAWRR HEGYYIYFED NAGVIVNPKG EMKGSAITGP VARECAELWP KLSSAASAIV

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Macromolecule #14: 60S ribosomal protein L14, putative

MacromoleculeName: 60S ribosomal protein L14, putative / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 19.340373 KDa
SequenceString:
MPRVELTEEE KLYISKKNLL FKRFVEPGRL CLIEYGPYAG KLCFIVDIVT ITRVIVDGAF ITGVPRMVIP LKRLKLLKER IKINKNCKS GFLRKTVNST KVLEEFNNSK LGKKMIIKKK RDEATDFERF QVYFAKRELK KKMTTIKNKK NGENKKVKKN V QKVKA

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Macromolecule #15: 60S ribosomal protein L27a, putative

MacromoleculeName: 60S ribosomal protein L27a, putative / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 16.765834 KDa
SequenceString:
MATRFKKNRK KRGHVSAGHG RVGKHRKHPG GRGKAGGLHH MRINFDKYHP GYFGKVGMRH LNLLKNRTYC PTINVDKLWG LLPEEKKKE FSENKDIAPV IDVTRKGYFK VLGNGKLKHN QPIVVKARYF SSVAEKKIKA VGGQCILVA

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Macromolecule #16: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 24.19766 KDa
SequenceString: MGAYKYIQEI WKKKQSDAMH FLLRVRTWEY RQLPVVHRVS KPSRPDKARR LGYKAIQGFV IYRVRVRRGD RKKRVKKGIV HGKPKHQGV HKQKSTRNLK SVAEGKVGKS ICGNLRVLNS YWVGQDAVYK YYEVILVDPN HNAVRNNPKI NWICNPVHKH R ELRGLTSA ...String:
MGAYKYIQEI WKKKQSDAMH FLLRVRTWEY RQLPVVHRVS KPSRPDKARR LGYKAIQGFV IYRVRVRRGD RKKRVKKGIV HGKPKHQGV HKQKSTRNLK SVAEGKVGKS ICGNLRVLNS YWVGQDAVYK YYEVILVDPN HNAVRNNPKI NWICNPVHKH R ELRGLTSA GKKYRGLRVK GHLSAKSRPS IRANWKRRQL IKLRKCR

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Macromolecule #17: 60S ribosomal protein L10, putative

MacromoleculeName: 60S ribosomal protein L10, putative / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 25.26548 KDa
SequenceString: MGRRPARCYR YCKNKPYPKS RYCRGVPDPK IRIYDMGRKK ADVNEFSGVV HLVSYEYEQI SSEALEAARI SANKYMITNC GKDNFHLRV RIHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPNGVVARV DIGQVLLSIR TKENFVSKAC EALRRAKYKF P GRQKVFVS ...String:
MGRRPARCYR YCKNKPYPKS RYCRGVPDPK IRIYDMGRKK ADVNEFSGVV HLVSYEYEQI SSEALEAARI SANKYMITNC GKDNFHLRV RIHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPNGVVARV DIGQVLLSIR TKENFVSKAC EALRRAKYKF P GRQKVFVS NKWGFTPFSK DEYQQYKKKG RIISDGVSCK FIREKGPLDK IYKDINTVLE S

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Macromolecule #18: 60S ribosomal protein L5, putative

MacromoleculeName: 60S ribosomal protein L5, putative / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 34.067352 KDa
SequenceString: MAYVKVVKNK AYFKRYQVKY RRRREGKTDY RARKALILQD KNKYNAQKLR FVVRKTNSQV ICQIACAHIE GDKILAEAKS KELIRYGIP VGLKNYAAAY ATGLLCARRF LKSLNLDTQF LGVEKVTGEI ENNEDKEDEE GRKPIKAFLD VGITRTTTGN R VFAALKGA ...String:
MAYVKVVKNK AYFKRYQVKY RRRREGKTDY RARKALILQD KNKYNAQKLR FVVRKTNSQV ICQIACAHIE GDKILAEAKS KELIRYGIP VGLKNYAAAY ATGLLCARRF LKSLNLDTQF LGVEKVTGEI ENNEDKEDEE GRKPIKAFLD VGITRTTTGN R VFAALKGA CDGGLNIPHG NNRFPGSKNE FNPEQLRKNI LGIHVAEYMK TLQEEDNDKY KTHFNDYLKN NIGADDIEQM YL NAHEKIR QNPEKESKDT KNVKKFVSKH EKPKKLNAKL RKKRVKEKLA LYVEKLQ

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Macromolecule #19: 60S ribosomal protein L18-2, putative

MacromoleculeName: 60S ribosomal protein L18-2, putative / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 21.508783 KDa
SequenceString:
MGIALKNVGR IKKHGRKHLV SKNPYLRLLV KLYNFLARRT NANFNKIIAK RLIMPKRYRP PLSLSKLQYH MANHPNDIAV VVGSITDDK RLFSLKQLKV CALRFTETAR KRIEDAGGEC LTFDQLALKY PTGKKCVLLR GPTKARTAEK HFGKAPGKPK S KARPYVRS KGRKFEKARG RRKSRAYKK

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Macromolecule #20: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 21.641096 KDa
SequenceString:
MSLTLQKRLA ASVLKCGKNK IWMDPNEISE ISLANSRFSI RKLYKEGLIL KKPQKVHSRA RVRLYKLAKR KGRHMGIGKR KGTKNARTN QKTLWIKRQR VLRRLLKRLR DSKKVDRHLY HSFYLKCKGN QFKNKRTLIE AIQREKNETL KKKAIADQLE A KRLKAQVL RNKRKLKKDK EVVA

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Macromolecule #21: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 21.793611 KDa
SequenceString:
MVKKIDNSLN TNIHQYHIVG RAIPTAKDKN PNVYRMCIFA KNDTNAKSRF WYFMKKINKL KKSNGELLAC EQIKERFPLR VKNYGVLLR YDSRTGTHNM YKEFRDTTKE GAIAQLYSEM AGRHRARASS INIIRISEIS SSLVRRPHIK QLLKRRLRFP A LHLPTLQK EYRKKFASKR PSTYRM

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Macromolecule #22: 60S ribosomal protein L21

MacromoleculeName: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 18.840244 KDa
SequenceString:
MGGKSRGKRS GTRYKFSKKF RKHGECTANK YLEKLNYGDY VDIVCDSTQQ KGMPFNYYHG KTGKIFHITK RGVGVLVNKR VKHRIEQKK VCVRIEHVRK SRCNEDFLLR KIKNAELIKE AKLKNEHINI KRKTEGPKPA AMIKVPPSKI ITIEPLPFYE E Y

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Macromolecule #23: 60S ribosomal protein L17, putative

MacromoleculeName: 60S ribosomal protein L17, putative / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 23.469926 KDa
SequenceString: MVKYAKKIRN LGKCAKGAGV DLRVHFKNTY EAARAIRRMK LLEAKKYLND VIEKKRCVPF RKYNGGVGRT NQAKEFNHTQ GRWPAKSCK FLLNILDNVQ ANAESRNLDV SKLRLIHVMV NKARPGRRRT YKAHGRINPF MSSPCHIQII AREINKPAKK S LLSNAEKQ ...String:
MVKYAKKIRN LGKCAKGAGV DLRVHFKNTY EAARAIRRMK LLEAKKYLND VIEKKRCVPF RKYNGGVGRT NQAKEFNHTQ GRWPAKSCK FLLNILDNVQ ANAESRNLDV SKLRLIHVMV NKARPGRRRT YKAHGRINPF MSSPCHIQII AREINKPAKK S LLSNAEKQ KKLPFKVTLK KLIKLNISNQ NRLKNKSVKK QQTVQ

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Macromolecule #24: 60S ribosomal protein L22, putative

MacromoleculeName: 60S ribosomal protein L22, putative / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 16.42293 KDa
SequenceString:
MVAKKDSTKV SKKLKKKVVK KTKIVKGLKK QKMNKSTKGI KYVLDCTKPV KDTILDISGL EQFFKDKIKV DKKTNNLKNK VVVTSDEYK IYITVHIPFS KRYIKYLAKK YIKMHQIRDF LRVIAKGKLA YEFKYFQLNN

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Macromolecule #25: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 22.152748 KDa
SequenceString:
MTQVEKKKKN VKNVNKHKII HKRVIKKGNR KYTNGNTKVK YRKQVVKKTI NKNVSKKKLL KENVDKDEKK KKMKISIRFK KPKTLIYAR NPKCPRIVKS CHSKTLDKYG LIKYPLTSEK AMKKIEEINT LVFMCDKRAN KKNIKKSVKN LFGIECDKVN V LNTLNGDK KAYVRLSGEH DALEVANKIG IL

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Macromolecule #26: 60S ribosomal protein L26, putative

MacromoleculeName: 60S ribosomal protein L26, putative / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 14.855709 KDa
SequenceString:
MKFNKQKSSS RRKMRKAHFT APAGLRRKIM SSKLSKELRL KYKTRSLPVR KDDEVLICRG HNHGREGKVV KINRKRYKIY VERVTREKV NGESTFIGIH PSNVVLTKLK VDKNRKKILD RKAAKEN

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Macromolecule #27: 60S ribosomal protein L24, putative

MacromoleculeName: 60S ribosomal protein L24, putative / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 19.29593 KDa
SequenceString:
MSQIKTTVKT EACSFSEYRI YPGRGQKYIA RDGKVYFYLS SKFASLALQK KKAAKLRWTQ TWRRNNKKTK IETTQRRRYK KTIKVQKAV CGLTVEDIRN RKAYVQSIEA KNKAKFGTKE KEDKKKTKDD KKKNLVHFQQ KKDFTKSKML NMAKSKMHKM M KK

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Macromolecule #28: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 16.78518 KDa
SequenceString:
MGKLLKPGKV IIILNGRRAG KKAVIVNTYE GQTRERPYSY CLVAGIEKHP LKVNKSMTKK KIVKRSKVKA FIKCINVNHI LPTRYQVAN DFDIKSLASD DVLKSKNKKK EVKKLGKIFR DKFLEPVNKK TGEVSKDISF LHKKLYF

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Macromolecule #29: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 14.437823 KDa
SequenceString:
MSNVSNALVW ELTRKSNCFI KKNKAGKKGV FLCDPLNVNY KNTPSSSGLV KSNSTNVTLK DGKVVFSVKT SKESNVVNQH FKAKNMKNV EKLLQQHGSF EKAKNKEKLL KKYKRLSKLY ETSHKKTN

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Macromolecule #30: 60S ribosomal protein L35, putative

MacromoleculeName: 60S ribosomal protein L35, putative / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 14.787824 KDa
SequenceString:
MSNVKAYELR TLKKKELLDK LDELKKELSG LRISKALGNS AKNSKIHGVR KNVARVLTVY NQKRKMELRQ LYKNKKFKPY NLRKKLTKN KRLQLSPKQK AAMTLRQKKK VQNFPQRKYL VVHKE

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Macromolecule #31: 60S ribosomal protein L29, putative

MacromoleculeName: 60S ribosomal protein L29, putative / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 8.041588 KDa
SequenceString:
MAKSKNHTNH NQNRKAHKNG IKKPKKHKFM SRKGLDPNFF RNQKYCLKGI QKKKKELKLK AKQEKNN

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Macromolecule #32: 60S ribosomal protein L7, putative

MacromoleculeName: 60S ribosomal protein L7, putative / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 30.593184 KDa
SequenceString: MADRYENQVE NELGKSMTSL RAKKVNKNKE LKQAAAKKIK TLKLINKKKR NDLRQRTLRY EEEYESERKK IIELKREARK NNCFYREAE KKVVFVIRLK GVNKLPPKVR SVFRLLRLLQ VHNGVFVKVN KATKEMLKIV EPYVTYGYPT LSTVRKLLYK R GYVRVGKV ...String:
MADRYENQVE NELGKSMTSL RAKKVNKNKE LKQAAAKKIK TLKLINKKKR NDLRQRTLRY EEEYESERKK IIELKREARK NNCFYREAE KKVVFVIRLK GVNKLPPKVR SVFRLLRLLQ VHNGVFVKVN KATKEMLKIV EPYVTYGYPT LSTVRKLLYK R GYVRVGKV RRYARKKIQD NADISKHLGK YNVHGIEDMV YQLYTCGPVF KKVNNFLWAF KLKPPRKGFK AKRHAFNEPR PG DWGNREA HINELINRMI

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Macromolecule #33: 60S ribosomal protein L30e, putative

MacromoleculeName: 60S ribosomal protein L30e, putative / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 11.79476 KDa
SequenceString:
MAKKSKKSAG DNINAKLQLV MKSGKYQFGR KSCLKALRTG KGKLVIVSSN CPSIQRSVIE YYAMLSKCGV HDYHGDNNDL GTACGKLFR ISCLVITDVG DSDIIKTNE

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Macromolecule #34: 60S ribosomal protein L31

MacromoleculeName: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 14.099854 KDa
SequenceString:
MVKGTVKKQK KTLKPVTKFI TINLSKLTHK VCYKRKAPRA IKEIRSIAGK LMHTKDVRLD VKLNKFIWSK GVRNPPKRVR VKLERKRNE DEDSKEKMYT IVEHVMVDSY KGLVNECEAN E

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Macromolecule #35: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 15.565751 KDa
SequenceString:
MAVKKVGKII KKRTKKFTRF QSNRFMRVKP AWRKPRGIDC RVRRRYKGTN LMPSIGYGSN KKTKFLLPNN KYKYVVKNVK EMEPLIMNH TKYCVQIAHN VSSKKRKQII ERAKQMNVSV INAKARLQKT EE

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Macromolecule #36: 60S ribosomal protein L35Ae, putative

MacromoleculeName: 60S ribosomal protein L35Ae, putative / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 16.306425 KDa
SequenceString:
MENVKVKKEQ TEVSQKKKVV KKVLKKNKKT SNKKLQAVRL YEKGVILGYK RSQRNQDPNF TLISIKNVNT KKHAQFYVGK RVAYVYRTT KHHDGVKIKC IWGKVCRTHG NSGVIRAKFK THIPPKAFGD RVRILMYPSN I

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Macromolecule #37: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 17.406967 KDa
SequenceString:
MAQRVHYRRH NHYNTKSNKV RPVRTPGGKL TIHVVKKKAG KPKCADCKTA IQGVKALRPA DNYRARRKNR TVARAYGGSI CARCIRERI MRAFLFEEQK CVRQVLKEKK KQEKKVKKVK KEKKKVNAKE VKKTATDNKK AKKVADKKKG K

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Macromolecule #38: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 12.82544 KDa
SequenceString:
MGRKSTIKPA TGIAVGFNSG HVVTKRNLKL HKKKKPFSKR KELIKDVVRE ITGFSPYEKR IIELIKIGTS ASTKRSLKYA KKKLGTHKR GKAKREEIQK VVILQRRKAA EKH

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Macromolecule #39: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 10.571592 KDa
SequenceString:
MGKAGKGTGS FGKRNGKTHF LCLRCGKRSY HLQKKKCASC GYPSAKKRRF NWSVKAKRRN TTGTGRCRYI KTLRRKLKNK FTEGSTPKP KQR

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Macromolecule #40: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 10.341729 KDa
SequenceString:
MPKQITDIRK FLKISRKPDT TAVIIMKKKS KTKKNTVITK LKLRTKKYLY TMVFADRKKA ERIENSLLPS LKRIYYPQKK VGKTVKK

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Macromolecule #41: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 6.540912 KDa
SequenceString:
MGSIKRFRLK QRLGKCRRQN RPVPHWYRLK KDTKIRYNTK RRHWRRTKLG L

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Macromolecule #42: Ubiquitin-60S ribosomal protein L40

MacromoleculeName: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 14.645169 KDa
SequenceString:
MQIFVKTLTG KTITLDVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAIEP SLAQLAQKY NCQKLICRKC YARLHPRATN CRNKKCGRTN QLRPKKKLK

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Macromolecule #43: 60S ribosomal protein L41

MacromoleculeName: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 5.10831 KDa
SequenceString:
MAHGASRYKK SRAKMRWKWK KKRTRRLQKK RRKMRQRSR

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Macromolecule #44: 60S ribosomal protein L37a

MacromoleculeName: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 10.826083 KDa
SequenceString:
MSRRTKKVGL TGKYGTRYGS SLRKQIKKIE LMQHAKYLCT FCGKTATKRT CVGIWKCKKC KRKVCGGAWS LTTPAAVAAK STIIRLRKQ KEEAQKS

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Macromolecule #45: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Plasmodium falciparum 3D7 (eukaryote)
Molecular weightTheoretical: 12.194902 KDa
SequenceString:
MVNVPKTRKT YCSNKCKKHT MHKVSQYKKG KERLSSLGRR RYDMKQKGFG GQTKPVFKKK AKTTKKIVLK LECTKCKKKR FQTMKRCKT FEMGADKKKK GGAVY

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Macromolecule #46: Mefloquine

MacromoleculeName: Mefloquine / type: ligand / ID: 46 / Number of copies: 2 / Formula: YMZ
Molecular weightTheoretical: 378.312 Da
Chemical component information

ChemComp-YMZ:
Mefloquine / medication*YM / Mefloquine

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Macromolecule #47: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 47 / Number of copies: 164 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #48: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 48 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
Details: 20 mM Hepes pH 7.4, 40 mM KAc, 10 mM NH4Ac, 10 mM Mg(Ac)2 and 5 mM 2-mecaptoethanol
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 0.02 nm / Pretreatment - Type: GLOW DISCHARGE / Details: Blot 2.5s before plunging
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 90 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated defocus max: 3.8 µm / Calibrated defocus min: 0.8 µm / Calibrated magnification: 104748 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.0 mm / Nominal magnification: 78000
Sample stageSpecimen holder model: GATAN LIQUID NITROGEN / Cooling holder cryogen: NITROGEN
TemperatureMin: 80.0 K / Max: 90.0 K
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Number grids imaged: 1 / Average electron dose: 20.0 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 3)
Startup modelType of model: EMDB MAP
EMDB ID:

2661

Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.3)
Final 3D classificationNumber classes: 4 / Software - Name: RELION (ver. 1.3)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.3)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.3) / Number images used: 43184

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Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: RIGID BODY FIT / Overall B value: 129.3
Output model

PDB-5umd:
Structure of the Plasmodium falciparum 80S ribosome bound to the antimalarial drug mefloquine

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