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- EMDB-7025: Cryo-EM structure of of the large subunit of Leishmania ribosome ... -

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Basic information

Entry
Database: EMDB / ID: EMD-7025
TitleCryo-EM structure of of the large subunit of Leishmania ribosome bound to paromomycin
Map dataprimary map
Sample
  • Complex: Leishmania donovani 91S ribosome LSU
    • Protein or peptide: x 42 types
    • RNA: x 8 types
  • Ligand: x 3 types
Function / homology
Function and homology information


maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome / large ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation ...maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome / large ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / RNA binding / membrane / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal L29e protein family / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e ...Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal L29e protein family / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / 60S ribosomal protein L35 / Ribosomal protein L30e, conserved site / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal L27e protein family / Ribosomal protein L30/YlxQ / Ribosomal protein L34e / Ribosomal protein L37ae / Ribosomal protein L14e domain / Ribosomal protein L19, eukaryotic / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal L40e family / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L44e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L7A/L8 / Ribosomal protein L10e signature. / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L14 / Ribosomal protein L30, N-terminal / Ribosomal protein L6, conserved site-2 / Ribosomal protein L14, KOW motif / Ribosomal L30 N-terminal domain / Ribosomal protein L14 / Ribosomal L37ae protein family / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L39e, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal_L31e / Ribosomal protein L30e signature 1. / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / 60S ribosomal protein L19 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L37e / Ribosomal protein L39e signature. / Ribosomal protein L21e / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L31e / Ribosomal protein L37ae/L37e / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L19e / Ribosomal protein L18e / Ribosomal L39 protein / Ribosomal protein L37e / Ribosomal L15 / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L7/L30 / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L32e / Ribosomal protein L32e superfamily / Ribosomal_L32e / Ribosomal protein L6 signature 2. / Ribosomal protein L32
Similarity search - Domain/homology
60S ribosomal protein L28, putative / 60S ribosomal protein L39, putative / 60S ribosomal protein L37a / 60S ribosomal protein L21, putative / 60S ribosomal protein L36, putative / 60S ribosomal protein L30 / 60S ribosomal protein L27A/L29, putative / 60S ribosomal protein L34, putative / Ribosomal protein L35Ae family protein / 60S ribosomal protein L23, putative ...60S ribosomal protein L28, putative / 60S ribosomal protein L39, putative / 60S ribosomal protein L37a / 60S ribosomal protein L21, putative / 60S ribosomal protein L36, putative / 60S ribosomal protein L30 / 60S ribosomal protein L27A/L29, putative / 60S ribosomal protein L34, putative / Ribosomal protein L35Ae family protein / 60S ribosomal protein L23, putative / Ribosomal protein L15 / 60S ribosomal protein L22, putative / 60S ribosomal protein L5, putative / 60S ribosomal subunit protein L31, putative / 60S ribosomal protein L44, putative / Ubiquitin-60S ribosomal protein L40 / 60S ribosomal protein L18a / 60S ribosomal protein L11 (L5, L16) / 60S ribosomal protein L10, putative / Ribosomal protein L1a, putative / 60S ribosomal protein L23a, putative / 60S ribosomal protein L17, putative / 60S ribosomal protein L9, putative / 60S ribosomal protein L18, putative / Ribosomal L38e family protein / Ribosomal L27e family protein / 60S ribosomal protein L29 / 60S ribosomal protein L7 family protein / 60S_ribosomal_protein_L35_putative/GeneDB:LmjF.26.2330/GeneDB:LmjF.26.2340 / Ribosomal protein L19e family protein / 60S_ribosomal_protein_L32/GeneDB:LmjF.21.1720 / Ribosomal protein L38, putative / 60S ribosomal protein L10, putative / 60S ribosomal protein L19, putative / 60S ribosomal protein L23a, putative / 60S ribosomal protein L7a / Large ribosomal subunit protein eL28 / 60S ribosomal protein L44, putative / 60S ribosomal protein L39, putative / 60S ribosomal protein L36, Putative / 60S ribosomal protein L9, putative / 60S ribosomal protein L32 / 60S ribosomal protein L37a / 60S ribosomal protein L11 (L5, L16) / 60S ribosomal protein L17, putative / 60S ribosomal protein L7, putative / 60S ribosomal protein L35, putative / Ribosomal protein L1a, putative / Ribosomal protein L15 / Ubiquitin-60S ribosomal protein L40 / Ribosomal L27e family protein / Ribosomal protein L35Ae family protein / 60S ribosomal protein L21, putative / 60S ribosomal protein L30 / 60S ribosomal protein L18a / 60S ribosomal protein L5, putative / 60S ribosomal subunit protein L31, putative / 60S ribosomal protein L27A/L29, putative / 60S ribosomal protein L23, putative / Large ribosomal subunit protein eL22 / 60S ribosomal protein L29 / Large ribosomal subunit protein eL34 / 60S ribosomal protein L18, putative / Large ribosomal subunit protein eL37 / Probable 60S ribosomal protein L14
Similarity search - Component
Biological speciesLeishmania donovani (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsShalev-Benami M / Zhang Y / Rozenberg H / Nobe Y / Taoka M / Matzov D / Zimmerman E / Bashan A / Isobe T / Jaffe CL ...Shalev-Benami M / Zhang Y / Rozenberg H / Nobe Y / Taoka M / Matzov D / Zimmerman E / Bashan A / Isobe T / Jaffe CL / Yonath A / Skiniotis G
CitationJournal: Nat Commun / Year: 2017
Title: Atomic resolution snapshot of Leishmania ribosome inhibition by the aminoglycoside paromomycin.
Authors: Moran Shalev-Benami / Yan Zhang / Haim Rozenberg / Yuko Nobe / Masato Taoka / Donna Matzov / Ella Zimmerman / Anat Bashan / Toshiaki Isobe / Charles L Jaffe / Ada Yonath / Georgios Skiniotis /
Abstract: Leishmania is a single-celled eukaryotic parasite afflicting millions of humans worldwide, with current therapies limited to a poor selection of drugs that mostly target elements in the parasite's ...Leishmania is a single-celled eukaryotic parasite afflicting millions of humans worldwide, with current therapies limited to a poor selection of drugs that mostly target elements in the parasite's cell envelope. Here we determined the atomic resolution electron cryo-microscopy (cryo-EM) structure of the Leishmania ribosome in complex with paromomycin (PAR), a highly potent compound recently approved for treatment of the fatal visceral leishmaniasis (VL). The structure reveals the mechanism by which the drug induces its deleterious effects on the parasite. We further show that PAR interferes with several aspects of cytosolic translation, thus highlighting the cytosolic rather than the mitochondrial ribosome as the primary drug target. The results also highlight unique as well as conserved elements in the PAR-binding pocket that can serve as hotspots for the development of novel therapeutics.
History
DepositionSep 9, 2017-
Header (metadata) releaseNov 1, 2017-
Map releaseDec 6, 2017-
UpdateApr 13, 2022-
Current statusApr 13, 2022Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6az3
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6az3
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_7025.png

Downloads & links

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Map

FileDownload / File: emd_7025.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationprimary map
Voxel sizeX=Y=Z: 1.02 Å
Density
Contour LevelBy AUTHOR: 0.065 / Movie #1: 0.065
Minimum - Maximum-0.40696242 - 0.62841886
Average (Standard dev.)0.0002886674 (±0.02751031)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 391.68 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.021.021.02
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z391.680391.680391.680
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.4070.6280.000

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Supplemental data

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Sample components

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Entire : Leishmania donovani 91S ribosome LSU

EntireName: Leishmania donovani 91S ribosome LSU
Components
  • Complex: Leishmania donovani 91S ribosome LSU
    • Protein or peptide: ribosomal protein uL2
    • Protein or peptide: ribosomal protein uL3
    • Protein or peptide: Ribosomal protein L1a, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L11 (L5, L16)Ribosome
    • Protein or peptide: 60S ribosomal protein L9, putativeRibosome
    • Protein or peptide: ribosomal protein eL6
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: ribosomal protein uL13
    • Protein or peptide: ribosomal protein eL13
    • Protein or peptide: 60S ribosomal protein L23, putativeRibosome
    • Protein or peptide: Probable 60S ribosomal protein L14
    • Protein or peptide: 60S ribosomal protein L27A/L29, putativeRibosome
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L10, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L5, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L18, putativeRibosome
    • Protein or peptide: Ribosomal protein L19e family proteinRibosome
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L17, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L22, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L23a, putativeRibosome
    • Protein or peptide: ribosomal protein uL24
    • Protein or peptide: ribosomal protein eL24
    • Protein or peptide: Ribosomal L27e family protein
    • Protein or peptide: 60S ribosomal protein L28, putativeRibosome
    • Protein or peptide: 60S_ribosomal_protein_L35_putative/GeneDB:LmjF.26.2330/GeneDB:LmjF.26.2340
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L7 family proteinRibosome
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal subunit protein L31, putative
    • Protein or peptide: 60S_ribosomal_protein_L32/GeneDB:LmjF.21.1720
    • Protein or peptide: Ribosomal protein L35Ae family proteinRibosome
    • Protein or peptide: 60S ribosomal protein L34, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L36, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L37
    • Protein or peptide: Ribosomal L38e family protein
    • Protein or peptide: 60S ribosomal protein L39, putativeRibosome
    • Protein or peptide: Ubiquitin-60S ribosomal protein L40
    • Protein or peptide: ribosomal protein eL41
    • Protein or peptide: 60S ribosomal protein L37a
    • Protein or peptide: 60S ribosomal protein L44, putativeRibosome
    • RNA: rRNA alpha
    • RNA: rRNA beta
    • RNA: rRNA gamma
    • RNA: rRNA delta
    • RNA: rRNA epsilon
    • RNA: rRNA zeta
    • RNA: rRNA 5.8S
    • RNA: rRNA 5S
  • Ligand: PAROMOMYCIN
  • Ligand: MAGNESIUM ION
  • Ligand: water

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Supramolecule #1: Leishmania donovani 91S ribosome LSU

SupramoleculeName: Leishmania donovani 91S ribosome LSU / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#50
Source (natural)Organism: Leishmania donovani (eukaryote)

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Macromolecule #1: ribosomal protein uL2

MacromoleculeName: ribosomal protein uL2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 27.520994 KDa
SequenceString: GKTVLSCRKG NGSVYQVHGH KRLGPAKLRI LDYAERHGYM RGVVKSIEHE AGRGAALARV EFRHPYKFRR VKELMVAPEG MFTGQSVFC GQKAPLAIGN VLPLGQITEG CIVCNVEAKP GDRGTLARAS GDYCIIISHN HETGRTRLKL PSGQKKSVPS T SRAMIGII ...String:
GKTVLSCRKG NGSVYQVHGH KRLGPAKLRI LDYAERHGYM RGVVKSIEHE AGRGAALARV EFRHPYKFRR VKELMVAPEG MFTGQSVFC GQKAPLAIGN VLPLGQITEG CIVCNVEAKP GDRGTLARAS GDYCIIISHN HETGRTRLKL PSGQKKSVPS T SRAMIGII SGGGRIEKPV LKAGNSFYRF RGKRNCWPKV RGVARNPVEH PHGGGNHQHI GHPSTVSRHS PPGQKVGLIA AR RTGRIRG GKAVKGA

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Macromolecule #2: ribosomal protein uL3

MacromoleculeName: ribosomal protein uL3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 45.741395 KDa
SequenceString: SHCKFEHPRH GHLGFLPRKR SRQIRGRARA FPKDDATQKP HLTSFMVFKA GMTHIVRDVD RPGSKVNKKE VVEPVTILEA PPMVIVGIV GYRQTPVGLK TIGTVWAHHT SVEFRRRYYK NWKQSAQLAF SRQKQFANTK EGKVAEARTL NAFAKKASVI R VIAHTQLR ...String:
SHCKFEHPRH GHLGFLPRKR SRQIRGRARA FPKDDATQKP HLTSFMVFKA GMTHIVRDVD RPGSKVNKKE VVEPVTILEA PPMVIVGIV GYRQTPVGLK TIGTVWAHHT SVEFRRRYYK NWKQSAQLAF SRQKQFANTK EGKVAEARTL NAFAKKASVI R VIAHTQLR KLRNHRVGVK KAHVQEIQIN GGNVAAKIAL AKSLLEKEVR VDSVFQQSEA CDVCSVTKGH GTEGVVKRWG VA CLPRKTH RGLRKVACIG AWHPARVMYT VARAGQHGYH HRTQLNKKIY QIGRSVAVEP NQATTTYDLT AKTITPMGGF VGY GTVRND YVMLKGSVSG PRRRVMTLRR PMAPQTSRHL KEKIVLKFID TSSKIGHGRF QTKKEKNQWF GPLKKDRIRR EERL RK

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Macromolecule #3: Ribosomal protein L1a, putative

MacromoleculeName: Ribosomal protein L1a, putative / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 40.523211 KDa
SequenceString: RPSVSVYSAS SDSVVGTCPL PAVFTAPIRN DIVKFVHTNM AKNSRQAYAV NRLSGMNHSA HSWGTGRAVA RIPRISGGGT STSGAGAFG NMCRGGRMFA PTKIFRRWHR KINLHQKRFA VVSALAASSV PSLVMSRGHR IDNVPEVPLV VEDSIQGYEK T KEAMAFLK ...String:
RPSVSVYSAS SDSVVGTCPL PAVFTAPIRN DIVKFVHTNM AKNSRQAYAV NRLSGMNHSA HSWGTGRAVA RIPRISGGGT STSGAGAFG NMCRGGRMFA PTKIFRRWHR KINLHQKRFA VVSALAASSV PSLVMSRGHR IDNVPEVPLV VEDSIQGYEK T KEAMAFLK AIAAIDDVNR VNDSREIRAG RGKMRNRRYV ARRGPMLVMP NNKGTRAFRN IFGLDLANVS ALNLLHLAPG GH VGRFVIW TKSAFEQLDK IFGTFTEASA VKKGFTLPAP MLTNTDVTRI MQSEEVRRVL KPKKLQAKKA SRYQTPTNGI KNR RLRLRL NPYVKRETAA AKGMRNVANR DARRKAKMAR VTKAKKAATK

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Macromolecule #4: 60S ribosomal protein L11 (L5, L16)

MacromoleculeName: 60S ribosomal protein L11 (L5, L16) / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 19.245344 KDa
SequenceString:
ANPMREIVVK KLCINICVGE SGDRLTRASK VLEQLCEQTP VLSRARLTVR TFGIRRNEKI AVHCTVRGKK AEELLEKGLK VKEFELKSY NFSDTGSFGF GIDEHIDLGI KYDPSTGIYG MDFYVVLGRR GERVAHRKRK CSRVGHSHHV TKEEAMKWFE K VHDGIIFQ

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Macromolecule #5: 60S ribosomal protein L9, putative

MacromoleculeName: 60S ribosomal protein L9, putative / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 21.118717 KDa
SequenceString:
VKVKSLCTLQ IPEGVTVDVK GRKVTVTGKR GTLTKDLTHL QLDLRVDKKN RTFTVIRWFG SKIPIACLNT TKAHVQNMIT GVTKGYRFK VRCAYAHFPI NVSVDGQNIE VRNFLGEKRV RRQLVPSSVK VSQTDPSKVK DEIIFDGNDL EQVSREAAVL H QMCLVKKK DIRKFLDGIY VQTKTNIE

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Macromolecule #6: ribosomal protein eL6

MacromoleculeName: ribosomal protein eL6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 21.208869 KDa
SequenceString: MAATKSAASA AKRKAAKKVS RKSPEYTTLR KSCAPGVIAI ILAGRFRGRR AVILKQLPHN GPLVVSGPMK YNGVPIRRID SRYVIATST KVDISSVDTA PITPEVFQRP KAEKLTKSEG DFMGDKQKAR AEKAAKKTSK AGKKTPVSDA RAQLQKKIDA A LIAAIKKD ...String:
MAATKSAASA AKRKAAKKVS RKSPEYTTLR KSCAPGVIAI ILAGRFRGRR AVILKQLPHN GPLVVSGPMK YNGVPIRRID SRYVIATST KVDISSVDTA PITPEVFQRP KAEKLTKSEG DFMGDKQKAR AEKAAKKTSK AGKKTPVSDA RAQLQKKIDA A LIAAIKKD AQGKEKAGYL RSVFTVKPGD APHRWNW

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Macromolecule #7: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote) / Strain: BPK282A1
Molecular weightTheoretical: 39.175203 KDa
SequenceString: MTVRSSPTAN LYLTYLLLSC PICTSPFPFA TLIRTHLHHR VAPAFAFLLF FGCAHVCACV LARLLLPYFH STFSASISRR AEKSMPGKE VKKATQPAKA ASPYKKPAVA SHFAARPKNF GIGQDVPYAR DLSRFMRWPT FVTMQRKKRV LQRRLKVPPA L NQFTKVLD ...String:
MTVRSSPTAN LYLTYLLLSC PICTSPFPFA TLIRTHLHHR VAPAFAFLLF FGCAHVCACV LARLLLPYFH STFSASISRR AEKSMPGKE VKKATQPAKA ASPYKKPAVA SHFAARPKNF GIGQDVPYAR DLSRFMRWPT FVTMQRKKRV LQRRLKVPPA L NQFTKVLD RASRNEALKL IKKYAPETRK ARRERLHKAA EEKKKDPKKT VSTRAPLAVV TGLQEVTRAI EKKQARMVVI AN NVDPVEL VLWMPNLCRA NKIPYAIVKD MARLGDAIGR KTATCVAITD VNAEDEATLK NLIRSVNARF LSRSDVIRRQ WGG LQLSLR SRAELRKKHA RNAGVDAAAI IQ

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Macromolecule #8: ribosomal protein uL13

MacromoleculeName: ribosomal protein uL13 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 25.346008 KDa
SequenceString: AFPSRKDASR AQRKSAKKHR PEIIVIDLKD HVLGRAAAVV AKQLLLGKKI TVVRCEQLNI AGTEIRNKIK YLQYLRKRKL TNPTKGPFH HRAPSDVFVR TVRSMLPRYT KRGMKALNSL VAYEGIPPNV VRTGGRVVIP RAQRHVCYRS ERPYTVLGNM C KHVGWKYS ...String:
AFPSRKDASR AQRKSAKKHR PEIIVIDLKD HVLGRAAAVV AKQLLLGKKI TVVRCEQLNI AGTEIRNKIK YLQYLRKRKL TNPTKGPFH HRAPSDVFVR TVRSMLPRYT KRGMKALNSL VAYEGIPPNV VRTGGRVVIP RAQRHVCYRS ERPYTVLGNM C KHVGWKYS DVVANLEKAR VEKASRHHEK QAKLREAWKA ARKEALAKMP KHNVEVLKKF GYA

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Macromolecule #9: ribosomal protein eL13

MacromoleculeName: ribosomal protein eL13 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 23.897861 KDa
SequenceString: PKGNNAIPHV HQKKHWNPCS SQKGNVKVFL NQPAQKHRRR RLRLLKAKKV FPRPLKALRP QVNCPTVRHN MKRRLGRGFS PAELKAAGL NPQYAATIGI RVDSRRKNKS EEGMNVNVQR LKTYMSKLVL FPMNHKKVQK GEASEEEVKA ATQDRSRFGD A AVGAVVYP ...String:
PKGNNAIPHV HQKKHWNPCS SQKGNVKVFL NQPAQKHRRR RLRLLKAKKV FPRPLKALRP QVNCPTVRHN MKRRLGRGFS PAELKAAGL NPQYAATIGI RVDSRRKNKS EEGMNVNVQR LKTYMSKLVL FPMNHKKVQK GEASEEEVKA ATQDRSRFGD A AVGAVVYP SAETPRAVSA EEKSMCVYAF LKKNHSAVRF FGARSARAAR KEAA

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Macromolecule #10: 60S ribosomal protein L23, putative

MacromoleculeName: 60S ribosomal protein L23, putative / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 14.421971 KDa
SequenceString:
ANVKGCRFRV SVALPVGAVV NCADNTGAKN LYVISVKGYH GRLNRLPSAA LGDMVMCSVK KGKPELRKKV LNAVIIRQRK SWRRKDGTV IYFEDNAGVI VNPKGEMKGS GIAGPVAKES ADLWPKISTH APAIV

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Macromolecule #11: Probable 60S ribosomal protein L14

MacromoleculeName: Probable 60S ribosomal protein L14 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.038955 KDa
SequenceString:
KSHYICAGRL VRILRGPRQD RVGVIVDIVD ANRVLVENPE DAKMWRHVQN LKNVEPLKYC VSVSRNCSAK ALKDALASSK ALEKYAKTR TAARVEAKKA CAASTDFERY QLRVARRSRA HWARKVFDEK DAKTPVSWHK VALKKMQKKA

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Macromolecule #12: 60S ribosomal protein L27A/L29, putative

MacromoleculeName: 60S ribosomal protein L27A/L29, putative / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 16.034684 KDa
SequenceString:
PTRFKKCRHQ RGSTFCGYGR VGKHRKHESG RGNAGGMHHH RINFDKYHPG YFGKLGMDHY HRKKNPMWKP TINLNNLSRL IAAEEAAKA TKGGTLPVVD LQSSGYAKLL GNGHIQVPCI VKARYVSKLA DKKIRKAGGA VVLQA

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Macromolecule #13: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 24.366773 KDa
SequenceString: GAFMYLNELW KKKSSDVMRF IQRIRSWEFR HQHTVVRLRR PTRPEKARML GYKTKQGFCV FRVRVRRGGR KRPVHKGITY GKPKTSGVL GMKLNKNNQA VAEQRLGKRF GNLRVLNSYW VNMDSTFKWY EVIAVDPMCK TIRRDPRINW IVNSVHKHRE Q RGLTSAGR ...String:
GAFMYLNELW KKKSSDVMRF IQRIRSWEFR HQHTVVRLRR PTRPEKARML GYKTKQGFCV FRVRVRRGGR KRPVHKGITY GKPKTSGVL GMKLNKNNQA VAEQRLGKRF GNLRVLNSYW VNMDSTFKWY EVIAVDPMCK TIRRDPRINW IVNSVHKHRE Q RGLTSAGR KHRGLRHKGH KASKLRPSYR AAWRRNNRIV FLRKR

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Macromolecule #14: 60S ribosomal protein L10, putative

MacromoleculeName: 60S ribosomal protein L10, putative / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 24.607186 KDa
SequenceString: MARRPSRCYR FCKNKPYPKS RFCRGVPDPK IRNFDIGRRR ATVDEFPVCI HVVSRELEQI ASEALEAARI QANKYMVKRA NKDVFHMRT RAHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPNGVCARV RIGQILLSMR TKEAYVPQAF EALRRAKMKF P GRQIIVMS ...String:
MARRPSRCYR FCKNKPYPKS RFCRGVPDPK IRNFDIGRRR ATVDEFPVCI HVVSRELEQI ASEALEAARI QANKYMVKRA NKDVFHMRT RAHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPNGVCARV RIGQILLSMR TKEAYVPQAF EALRRAKMKF P GRQIIVMS KYWGFTNILR TEYEALRDAG KLEQRGTHCK LIAPKGKITM RNVMA

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Macromolecule #15: 60S ribosomal protein L5, putative

MacromoleculeName: 60S ribosomal protein L5, putative / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 34.100633 KDa
SequenceString: MPFVKVVKNK AYFKRFQVKY RRRREGKTDY HARRQMVLQD KTKFGSPKYR LVVRITNKDI IAQIVQAKIV GDEVVMAAYA HELPAFGIE HGLTNYAAAY ATGLLLARRT LAKLGIADKF QGAKEADGSY SAVRTKKDDE GDDEERFPFK AILDVGLART T TGARVFGV ...String:
MPFVKVVKNK AYFKRFQVKY RRRREGKTDY HARRQMVLQD KTKFGSPKYR LVVRITNKDI IAQIVQAKIV GDEVVMAAYA HELPAFGIE HGLTNYAAAY ATGLLLARRT LAKLGIADKF QGAKEADGSY SAVRTKKDDE GDDEERFPFK AILDVGLART T TGARVFGV LKGAVDGGMA VPHRPNRFPG YNKEKSSLDA KVHRDRIFGK HVAEYLKQVK EEASSNPDEK CVQFSRYMAA KV LPESIEG MYKKAHAAIR ADPSKSLPKK AKKEGVAHKS YKTKKLSGAE KRAAAKAKVA AIRERLGK

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Macromolecule #16: 60S ribosomal protein L18, putative

MacromoleculeName: 60S ribosomal protein L18, putative / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 21.939883 KDa
SequenceString: GVDLTGISKK SRVIRHHTYS TNPYIKLLIK LYKFLAKRTS SGFNKVVYQR LIKSRSNRAP ISLSRIAVVM KRKAVFTAKS KAAPIAVVV GDVLDDVRMA RIPAMRVCAL RFSKSARQSI VAAGGECLTF DQLAMIAPTG KNTYLLRGRK SGRESVRHFG A SGVPGSHS ...String:
GVDLTGISKK SRVIRHHTYS TNPYIKLLIK LYKFLAKRTS SGFNKVVYQR LIKSRSNRAP ISLSRIAVVM KRKAVFTAKS KAAPIAVVV GDVLDDVRMA RIPAMRVCAL RFSKSARQSI VAAGGECLTF DQLAMIAPTG KNTYLLRGRK SGRESVRHFG A SGVPGSHS KPYATNRGKE TKRGRRTGRS YKRKAFRHV

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Macromolecule #17: Ribosomal protein L19e family protein

MacromoleculeName: Ribosomal protein L19e family protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 22.901018 KDa
SequenceString:
VSLKLQARLA SSILGCGRAR VWLDPNEAVE IQNANSRKSV RKLIKDGFII RKPVKVHSRA RWRKMKEAKD MGRHNGVGRR EGSREARMP SKELWMRRLR ILRRLLRKYR ADKKIDRHVY RDLYVRAKGN VFRNKRNLVE HIHKIKNEKK KERQLAEQLA A KHLRDEQN RNKARKQELR KREKERERAR R

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Macromolecule #18: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 20.703172 KDa
SequenceString:
VKPHLRHYQV VGRESPSEKN PEPTVYKFEV FAPNFVVAKS RFWRMMRVKN KVKATHGDVL SCKVVKDAKL VARNYLVDIA YYSQRCGYT RMVKEFRDVS KTGAVSQAYH DLASRHRARY HNIEVLNVKS IPDHEVKHLS IAQYHAPNLS FPLLQRRIKA A RKDRAIFV KKNTKRAVVA

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Macromolecule #19: 60S ribosomal protein L21, putative

MacromoleculeName: 60S ribosomal protein L21, putative / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.437443 KDa
SequenceString:
VHSYGYKSGT RHLFAKKFRK HGAPSVSTIL TNIKVGDYVD VVADSAVREG MPHKYYHGRT GIVWNVTPRG VGVIINKPVR TRTLRKRIC VRFEHVRKSR CQEAFKAKEH QFQAYLAAKK AGKALPPLKK SSRMGGIVRP KNVEVLARRV ADYEA

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Macromolecule #20: 60S ribosomal protein L17, putative

MacromoleculeName: 60S ribosomal protein L17, putative / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.828902 KDa
SequenceString:
THYSRKPQVS SKSAKAKVSD LRCHYKNTFE TANVINGMPL RKAQQLYRQV LAKTRCIPFK RYNGKIGRTA QAKEWGQTKG RWPRKSVVA MMSLLKNAEA NAIEKGLDPN QMVIKHVQVD QAARMRRRTY RAHGRITPYM CSPCHVQLFM SEKKE

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Macromolecule #21: 60S ribosomal protein L22, putative

MacromoleculeName: 60S ribosomal protein L22, putative / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 14.042504 KDa
SequenceString:
VAVRAKVGSR SYVRQKQLAK GKKVFKIDCS IPAADGIFSE DVLGNFEQFF QDNTKLNGRK GKLSDKVRLS MNDNVLTIST TMAYRKKYF KYLTKKFLKK KDLRDWIRIL ATGKGTYQLK YF

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Macromolecule #22: 60S ribosomal protein L23a, putative

MacromoleculeName: 60S ribosomal protein L23a, putative / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 13.461783 KDa
SequenceString:
AKSYTRPQFR RPHTYRKPAM AKPSNRVTVE SKDIAAFSVI RYPLTTDKAM KKIEENNTLT FIVDSRANKT EIKKAMRKLY QVKAVKVNT LIRPDGLKKA YIRLSAAHDA LDTANKIGL

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Macromolecule #23: ribosomal protein uL24

MacromoleculeName: ribosomal protein uL24 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 13.874408 KDa
SequenceString:
ASIKCGSRRK ARRAHFQAPS HVRRVLMSAP LSKELRAKYN VRAMPVRKDD EVIVKRGTFK GREGKVTACY RLKWVILIDK VNREKANGS TVAVGIHPSN VEITKLKLTH HRKSILERKD RS

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Macromolecule #24: ribosomal protein eL24

MacromoleculeName: ribosomal protein eL24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 7.988512 KDa
SequenceString:
MRTIECEFSH FAVHPGHGRR YVPFAFLSTK PVLTFSRPKC FALYMRKKNP RFIPWTRTYR RIHRK

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Macromolecule #25: Ribosomal L27e family protein

MacromoleculeName: Ribosomal L27e family protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 15.223005 KDa
SequenceString:
KFLKPGKVVI VTAGRYAGHK AVIVQNSDIA TKERPYGRAL LAGIKKYPKK VVRGMSKQTI ARRSQVGVFL RVVNHKHFLP TRYNVDMSK ELRGKINVSD ASKRSRSKRL VRHVFQARYN AGSSMWFFQR LRF

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Macromolecule #26: 60S ribosomal protein L28, putative

MacromoleculeName: 60S ribosomal protein L28, putative / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 15.525923 KDa
SequenceString:
SHSVDLQWIL VRQNSRFLQK RGGIRMSNDP FNNNGNWTKR HCGFLNEKAA VVKPAKGGAI CVTVKDGSSN NKPRQTYRKT VHAAGVRAS DVSRAVAAVR PDLADVSFRR ARRMACIASR TAKVAAARKA RSEKIKFSRK S

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Macromolecule #27: 60S_ribosomal_protein_L35_putative/GeneDB:LmjF.26.2330/GeneDB:Lmj...

MacromoleculeName: 60S_ribosomal_protein_L35_putative/GeneDB:LmjF.26.2330/GeneDB:LmjF.26.2340
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 14.987824 KDa
SequenceString:
SHHMKIKDLR EKSKDDLLKT LTEYKKELSQ LRVVQQTGGA ETRLGRIRPI RKSIARILTV LNQNERSNLK MFYADRKLRC KTPKVLRAK LTHRRRLALK ENEKNRKTSR QMRQAHKFPK RVYAVK

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Macromolecule #28: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 7.788074 KDa
SequenceString:
AKSKNHTNHN QSSKNHRNGI KGPMPLHLHN SKRGSWLPAL VNARRVRKHN QKAALKKRRE RIAAFAAK

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Macromolecule #29: 60S ribosomal protein L7 family protein

MacromoleculeName: 60S ribosomal protein L7 family protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 26.313074 KDa
SequenceString: AAFKQQQTEN FKKAVVARKA AKAALKKTAY LRARKYSREY RGAEKKLVTL RRQAASHGNY YLEAKPKVAV VTRIRGIAKV NPKQRKILQ LLRLRQIFNT VFVKMNKPME NMLRAVEPYI AYGYPSLATV RAMVYKRGYL KINGQRVKIT DNQMIKDKYN N ADIVCAED ...String:
AAFKQQQTEN FKKAVVARKA AKAALKKTAY LRARKYSREY RGAEKKLVTL RRQAASHGNY YLEAKPKVAV VTRIRGIAKV NPKQRKILQ LLRLRQIFNT VFVKMNKPME NMLRAVEPYI AYGYPSLATV RAMVYKRGYL KINGQRVKIT DNQMIKDKYN N ADIVCAED MVNQIYTCGK HFRTVTHGMW PFKLAPPAGG MRQKRRHFVE GGDYGNRDTL INRFLARMI

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Macromolecule #30: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 10.122675 KDa
SequenceString:
VDTINAKLQL VMKSGKYVLG TQQALTTLRQ GRSKLIVISN NCPPIRRAEV EYYCTLSKTP IHHYSGNNLD LGTACGKHFR TCVLSVTNV GDS

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Macromolecule #31: 60S ribosomal subunit protein L31, putative

MacromoleculeName: 60S ribosomal subunit protein L31, putative / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 13.503024 KDa
SequenceString:
TRASLRKRTG RKPDAVSMEA TIHLSKLLKK KTFSKRAPLA IKRIKAFVGR LMKTKDNRID ASLNTYIWHK GVKGVPGRVR VLIQRKSET TEGNKHKHFY TVISNVPVAS FKGLTTKTVE

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Macromolecule #32: 60S_ribosomal_protein_L32/GeneDB:LmjF.21.1720

MacromoleculeName: 60S_ribosomal_protein_L32/GeneDB:LmjF.21.1720 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 15.02965 KDa
SequenceString:
VKPTVSKAIV KKRTKRFTRH RYELFPQLSS SWRKPRGEDS PVRRRYKGQK AMPNKGYGSD RATKYITPSG FRSFPINNVE DLYMLVMQN RKYAGVISHT VGARKRKAIA RKAMELDVRL ANGNAKLRKI A

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Macromolecule #33: Ribosomal protein L35Ae family protein

MacromoleculeName: Ribosomal protein L35Ae family protein / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 14.336777 KDa
SequenceString:
KTSRVNRNRK APRLYMKGTL AGYTRGLHGQ TKQTALVRVE NVNTREDATW YVGKRVCYVY HGKKVKRCVR WSKAPARRST TRALWGRVT RPHGNAGMMR VKFNGASVPA SAIGRRIRVY LYPSQI

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Macromolecule #34: 60S ribosomal protein L34, putative

MacromoleculeName: 60S ribosomal protein L34, putative / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 14.640328 KDa
SequenceString:
CPRVQYRRRM HYATRGNRMK MVRTPGNKLV MQKRAKRSQG IHTPWVLGHK RLGGTKALRH IDARLASRHE KSVSRAYGGV LSHDQVRDR VVRAFLVEEQ RIVKQALKEH SKMKLSHKRT ANKKKS

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Macromolecule #35: 60S ribosomal protein L36, putative

MacromoleculeName: 60S ribosomal protein L36, putative / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 11.050092 KDa
SequenceString:
APTPRTGIIA GFNKGHVTTR RPRQPSPNDR FAVPHKHLRA VKAIIADLVG LSPLEKRVQE FLRVGKEKRA LKYCKKRLGD FTAAKKKRS KMEEALRH

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Macromolecule #36: 60S ribosomal protein L37

MacromoleculeName: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 9.531135 KDa
SequenceString:
TKGTTSMGQR HGRTHILCRR CGRNSYHVQW ERCAACAYPR ASRRRYNWSV KAIKRRRTGT GRCRYLKVVN RRIANHFKTP

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Macromolecule #37: Ribosomal L38e family protein

MacromoleculeName: Ribosomal L38e family protein / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 8.761474 KDa
SequenceString:
PREIKTLKEF LAICSRKDAR CVKVKHNPSA TKFKVRCSRY LYTLVVNDKK KADKIERSIH PSVKKIAVTA RSHAKT

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Macromolecule #38: 60S ribosomal protein L39, putative

MacromoleculeName: 60S ribosomal protein L39, putative / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 6.373649 KDa
SequenceString:
GRFKPLAVKK KYAKKMNQNK PVPYWIRLRT GNRIKWNEKR RHWRRTKLNY

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Macromolecule #39: Ubiquitin-60S ribosomal protein L40

MacromoleculeName: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 5.912278 KDa
SequenceString:
MEPTLVALAK KYNWEKKVCR RCYARLPVRA TNCRKKACGH CSNLRMKKKL

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Macromolecule #40: ribosomal protein eL41

MacromoleculeName: ribosomal protein eL41 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 4.2593 KDa
SequenceString:
GTVSRPRGMR PKWHKKRIKR LKLRRRRMRQ RSK

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Macromolecule #41: 60S ribosomal protein L37a

MacromoleculeName: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 10.092869 KDa
SequenceString:
AKRTVKMGVM GRYGTRYGAN PRKRAKKLEV SQHAKHFCSF CGKFAFRRKA VGIWRCDGCS KTVAGGAYTL STPNNSTVRS TVRRLRELA K

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Macromolecule #42: 60S ribosomal protein L44, putative

MacromoleculeName: 60S ribosomal protein L44, putative / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 11.141353 KDa
SequenceString:
VNYPKKKVMH CGDARCNAHK SFKVVQYKAG KARLFARGKR RYDRKQSGYG GQTKPVFHKK AKTTKKIVLK LQCSGCKSIR QVVLKRTKH FELNDKK

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Macromolecule #43: rRNA alpha

MacromoleculeName: rRNA alpha / type: rna / ID: 43 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 575.707562 KDa
SequenceString: AGACCUGAGU GUGGCAGGAC UACCCGCCGA ACUUAAGCAU AUUAC(OMU)CAGC GGAGGAAAAG AAAACA(A2M)CCG UG AUUCUCU CAGUGAGCGG CGAGCGAAGA GGGAACGAAC UCGUUGCCGA AUCGGGUCUU ACAAGGCCUU GAGUUGUGGC ACA AAUAUC ...String:
AGACCUGAGU GUGGCAGGAC UACCCGCCGA ACUUAAGCAU AUUAC(OMU)CAGC GGAGGAAAAG AAAACA(A2M)CCG UG AUUCUCU CAGUGAGCGG CGAGCGAAGA GGGAACGAAC UCGUUGCCGA AUCGGGUCUU ACAAGGCCUU GAGUUGUGGC ACA AAUAUC AUUUCGUGGU UGGUGCAGGA GUGGCGAAAU UCAAGCGCAA AGCAAACCCG UUGCUGAAUA CAACCCUUCA UGUG (A2M)GUAU UGAGCCAAAG AAGGUGUUAG CCCAUUGAGC CAUAAACCUG AGCGCCUCAU GAACUGUAUU AAGCGAGUAG C ACUGUUUG GGAAUGCAGU GUCAAGUUGG CAGGUAUUUG UCUGCCAAAG UUAAAUACAG AGUAGGAAGA CCGAUAGCGA AC AAGUAGC GUGAGCGAAA GUUUGAAAAG CACUUUGGAA AGAGAGUGA(A2M) AUAGAACCUG AAGUCGUGAC AAAGACAAU GGAAGUACCU CCAUUUCAAC GGCCAGAACC AGCCCUACUG CAUUAUCCUG UCGGGGGUCA AAAUCCGGCG GGCAACGAAG UGCAAGAAU UUUGGGUCUU GGUGAAAUGA GCGGAGUAUU UGAGCUGUGC ACGGCGCAAG CCGCACGCAC UGGUUUCUUC U CCGUGAUG CUCCAUGUUG GCGAUGGAAA UGGGGUGCCC CCACCCGUCU UGA(A2M)AC(A2M)CGG ACCAAGGAGU (OMC)A(A2M)ACAGAC GCGCAAGGGA GGAGAUAUGG UUGCCAGUAC UUCUCUCGUA CUGAAAGGGA GAUGCAAAAU GCA UCUGUG GUGUGCACAC GUCAAAUCCG UUUGAUUUGC CCAACACCGA CCGGCCCUCC GUGGGUUGCG UUGGAGUGUG CC (OMU)G(OMU)UUGG ACCC(OMG)A(A2M)AGG UGGUGAACUA UGCCUGAACA AGGUGAAGCC CGGGGAAACC CAGGUGG AG GCCUGUAGCG AUGCUG(A2M)CGU GCAAAUCGCU CGUAUGAUUU GGGU(A2M)UCG(OMG)A GCGAAAGACU CAUCGA ACC ACCUAGUAGC UGGUUCACAU CGAAGUUUCC CUCAGGAUAG CUGGUGCUAG UAGAAGUAUU GUGCGGUAAA GCAAAUG AU UAGAGGCAUU GGUGUUCUUA GAAUAUCGAC CUAUUCUCAA ACUUUAAA(OMU)G UGCAAACAAA CCGUGCCUUA GCCA ACUGC AAGGCCGGAA AGAAAAGAAU UAGAGGCACC AAGUGGGCCU UCUUCGGUAA GC(OMG)GAGCAGG CGAUGCGGAA U GAACCGCU UAGGGAUAUG UGGCGUCAAA ACUUAUGGGC UCAUUC(OMU)CGA UACGUGAAAA GGGUGUUGGU GGAUUGGA C AGUUGGACGG UGGCCAUGGA AGUCGGCAUC CGCUAAGGAG UGUGUAACAA CUCACCAACC GAAUCCACCG GCCCCGAAA AUGGA(OMU)GACG CUUAAGCCCA AUUAGUGAUU GCCCAUUAUU CCUUUGGUAA GGGCGGAAAC AAACUCUUGG AGAAAG UGU AGCCCUUCCG AUGUAGAUGU GGCCUGGAGG UUAGGACGAA GCUUAUGGCG UGAGCCUAAG AUGGACCGGC CUCUAGU GC A(OMG)AU(OMC)UUGGU UGGCGU(A2M)(OMG)CA AAGAUCUAAC GGAGAUAUAC UCAACAUGCA ACGUUGGAUA CU GGAGCGG GGAAGGAUUU CGUGCCAACG GCACUCGUAC ACGA(OMG)UUGUU CGGAAACUGA GCACAACGUU AUAUCGU (OMU)U UGUUAGGAAA GUGAAGGUGU GUCGGCGGCA GCUGCUUUCG GGUAGCUGUU CGACUGGCCA UAACUGAAAA GGGG CAACA GAGAACCUGG GAUUAUAUUC CAAAAAGAAA UUGCAUGUGG G

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Macromolecule #44: rRNA beta

MacromoleculeName: rRNA beta / type: rna / ID: 44 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 492.460781 KDa
SequenceString: CCCAACUGCA GACCGUACUC AUCACCGCAU CAGGUCCCCA AGCAUAGAAU GCUCUGGUAC AUAGAGAAA(OMG) G(OMU) ACACUCA GGGAAGUCGG CAAAAUAGAU GCGUAAGUUC GCAAGAAGCA UUGGCUCUGA GGGCAAAGUC AGAGAAAACC GGA AGGCCA ...String:
CCCAACUGCA GACCGUACUC AUCACCGCAU CAGGUCCCCA AGCAUAGAAU GCUCUGGUAC AUAGAGAAA(OMG) G(OMU) ACACUCA GGGAAGUCGG CAAAAUAGAU GCGUAAGUUC GCAAGAAGCA UUGGCUCUGA GGGCAAAGUC AGAGAAAACC GGA AGGCCA GUGUCCUCUC GCAUGCAGCC GUUCUUCAGA UGUUGUUUUG GCGCUCGCCG CCUUAGCACG CUGAUAGCGA CUGU ACUGU CGUCUGUGAA GGCUACCUUC CCGAGGCGUC GUAACCCGGC GUCUCACUCU GGCACGGGCC GGUCUUGGCG GGUUC ACGC UCGCCUUGUG CCGCGUCCCA CAAAUUGCCA ACUCAGAACU G(OMC)UUACGGCG GGGAAUCCAA CUGU(A2M)UAA U UAAAACAUAG GUUUGUGAUG CAUCCAAGUG GUGUAUAUCG CAAACUGAUU UCUGCC(OMC)AGU GCUCUGAAUG UCAAC GUGA CGAGAUUCAC CGACGCGCGG GUAAACGGCG GGAGUA(A2M)CUA UGACUCUCUU AAGGUAGCCA A(A2M)UGCCUC (OMG) UCUUCCAAUU AGAGACGCGC AUGAA(OMU)GGAU UAAUG(A2M)G(A2M)UU CCCUCUGU(OMC)C CGAGUU (A2M)CU AUCUAGCGAA (A2M)CGACAGUCA AGGGAACGGA CUUG(A2M)AGGGC UAAGCGG(OMG)GA AAGAAGACCC U (OMG)UUGAGUU UG(A2M)C(OMU)CCAGU CUGGCUCUGU GCGGCGACAU CUGAGGUGUA GUAUAGGUGG AAGCGCAAGC GCAAAUGAA AUACCACCAC UCGGAACGUU GCUUCACUUA UCGAAUGAAG AGACCAAUGG GUUUAGCGUA GUCUUCGGGC U AUGCACCG UCUAGGUUUG GGAGUAAUUU UAUGGCGGUA ACCCCGUCCC CACGGGAAAG GGUGGUGUGC UUUUUUCUGC GU GCGUCUU CACGGGCGUC GCGCGGGCCC CGCUCUUUUU UAUUACGUUU CCUUCGGGGG GCGUGACUCG UGGGUACCCA ACC GUGCUG UAAACUGAAC CAACGGGAUU GCCUCGUGCA GUUCCGGAGU CUUGUUUCGA GACAUCUGCC AGAUGGGGAG UUUG GCUG(OMG) GGCGGCAUAU CUGUUACACG (A2M)CAACGCAGG (OMU)(OMG)UCCUAAGG CGAGCUCAGU GGGAACAGA AAUCUCACGU AGAACACAAG CGUAAAAGCU UGCUUGAUUA ACGAUU(OMU)CCA GUA(OMC)GAAUCG AGACUGCGAA AG CAAGGCC (A2M)GCGAUCCUU UGCGAAGAAU AGGAAAUAUG AACAAAUCAU ACCA(OMG)A(OMG)GUG UCAGAAUAAU U AC(OMC)ACAG(OMG) GAUAACUGGC UUGUGGCGGC CAAGCGUUCA UAGCGACGUC GCUUUUUGAU CCUUCGAUGU CGG (OMC)UCUUC CUAACCUAGC GCCGCAGAAG ACGCUAAGGG UUGGAU(OMU)(OMG)UU CACCCACUG(A2M) CAGGGAAC G UG(A2M)GCUGGGU UUAGA(OMC)CGUC GUGAGACAGG UUGGUUU(OMU)AC CCUACUUAGC UGGAAAUUGC GAGAAAA AG AUUAUCCGUG CAAAGCGGAC GUCGGGUCGU UCUUGGGCCU GGCGAGCGGU GGGUAGUACU UGGUACGCUC AUCACC

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Macromolecule #45: rRNA gamma

MacromoleculeName: rRNA gamma / type: rna / ID: 45 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 67.56875 KDa
SequenceString: AGUGGUAA(OMU)G CGAAACACUG CCAGGUAACA AAUCAAUCCU CCCACGGUGA GCUUUCUUUU CACCAUAAUC CACAUC UCC GGCUUUGCUG GGGCUUGGGC CUUUUUACUU CUCGCGUUGU UCGGAGCGGG GGCUCAAGAU UGAAAAAUGC AGCUCUC CC UACGUACUGU ...String:
AGUGGUAA(OMU)G CGAAACACUG CCAGGUAACA AAUCAAUCCU CCCACGGUGA GCUUUCUUUU CACCAUAAUC CACAUC UCC GGCUUUGCUG GGGCUUGGGC CUUUUUACUU CUCGCGUUGU UCGGAGCGGG GGCUCAAGAU UGAAAAAUGC AGCUCUC CC UACGUACUGU CAUUGUUGUG AGUUCUGCGC AUUAACGCAA AAACCUGGGG UGU

+
Macromolecule #46: rRNA delta

MacromoleculeName: rRNA delta / type: rna / ID: 46 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 59.144129 KDa
SequenceString:
GUGAGAUUGU GAAGGGAUCU CGCAGGUAUC GUGAGGGAAG UAUGGGGUAG UACGAGAGGA ACUCCCAUGC CGU(OMG)CC UCU AGUUUCUGGG GUUUGUCGAA CGGCAAGUGC CCCGAAGCCA UCGCACGGUG GUUCUCGGCU GAACGCCUCU AAGCCAG AA GCCAAUCCCA AGACCAGAUG CCCAC

+
Macromolecule #47: rRNA epsilon

MacromoleculeName: rRNA epsilon / type: rna / ID: 47 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 42.869438 KDa
SequenceString:
UACGUCCCUC UCCAAACGAG AGAACAUGCA UGGGCUGGCA UGAGCGGCAU GCUUCACUUC GGUGGGGCUC GAGGGGCAUU UACGUCCCG AGGCGCUGAA CCUUGAGGCC UGAAAUUUCA UGCUCAGGGA CACA

+
Macromolecule #48: rRNA zeta

MacromoleculeName: rRNA zeta / type: rna / ID: 48 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 22.72559 KDa
SequenceString:
AUCGAAUCGC CACCUACAAG ACUGGAGCUU GCUCCCUCGA AGGCGCCAAG UAUAUUCAUG AUCACAAGAC A

+
Macromolecule #49: rRNA 5.8S

MacromoleculeName: rRNA 5.8S / type: rna / ID: 49 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 54.98957 KDa
SequenceString:
AACGUG(OMU)CGC GAUGGAUGAC UUGGCUUCCU AUUUCGUUGA AG(A2M)ACGCAGU AAAGUGCGAU AAGUGGUAUC AA UU(OMG)CAGA AUCAUUCAAU UACCGAAUCU UUGAACGCAA ACGGCGCAUG GGAGAAGCUC UAUUGUGUCA UCCCCGUGC AUGCCAUAUU CUC(A2M)GUGUCG AAC

+
Macromolecule #50: rRNA 5S

MacromoleculeName: rRNA 5S / type: rna / ID: 50 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 37.923516 KDa
SequenceString:
GAGUACGACC AUACUUGAGU GAAAACACCA UAUCCCGUCC GAUUUGUGAA GUUAAGCACU CACAGGCUCA GUUAGUACUG AGGUCAGUG AUGACUCGGG AACCCUGAGU GCCGUACUC

+
Macromolecule #51: PAROMOMYCIN

MacromoleculeName: PAROMOMYCIN / type: ligand / ID: 51 / Number of copies: 10 / Formula: PAR
Molecular weightTheoretical: 615.628 Da
Chemical component information

ChemComp-PAR:
PAROMOMYCIN / Antimicrobial, medication*YM / Paromomycin

+
Macromolecule #52: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 52 / Number of copies: 34 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #53: water

MacromoleculeName: water / type: ligand / ID: 53 / Number of copies: 1019 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 141028

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