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- EMDB-4242: Human cap-dependent 48S pre-initiation complex -

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Basic information

Entry
Database: EMDB / ID: EMD-4242
TitleHuman cap-dependent 48S pre-initiation complex
Map datahuman 48S pre-initiation complex, reconstituted in the presence of capped mRNA, eIF4B and eIF4F, displaying eIF2 ternary complex, eIF3 and eIF4B
Sample
  • Complex: human cap-dependent 48S translation pre-initiation complex
    • Complex: human cap-dependent 48S translation pre-initiation complex
      • Protein or peptide: x 46 types
      • RNA: x 2 types
    • Complex: mRNAMessenger RNA
      • RNA: x 1 types
    • Complex: Eukaryotic translation initiation factor 4B
      • Protein or peptide: x 1 types
  • Ligand: x 1 types
Function / homology
Function and homology information


positive regulation of mRNA binding / viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 3 complex, eIF3m / Response of EIF2AK1 (HRI) to heme deficiency / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Recycling of eIF2:GDP / eukaryotic translation initiation factor 2 complex / PERK regulates gene expression ...positive regulation of mRNA binding / viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 3 complex, eIF3m / Response of EIF2AK1 (HRI) to heme deficiency / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Recycling of eIF2:GDP / eukaryotic translation initiation factor 2 complex / PERK regulates gene expression / eukaryotic translation initiation factor 3 complex / eukaryotic translation initiation factor 4F complex / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / eukaryotic 43S preinitiation complex / cytoplasmic translational initiation / translation factor activity, RNA binding / protein-synthesizing GTPase / formation of cytoplasmic translation initiation complex / formation of translation preinitiation complex / Deadenylation of mRNA / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / protein tyrosine kinase inhibitor activity / eukaryotic 48S preinitiation complex / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / IRE1-RACK1-PP2A complex / positive regulation of endodeoxyribonuclease activity / positive regulation of Golgi to plasma membrane protein transport / TNFR1-mediated ceramide production / negative regulation of DNA repair / negative regulation of RNA splicing / M-decay: degradation of maternal mRNAs by maternally stored factors / laminin receptor activity / oxidized purine DNA binding / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / supercoiled DNA binding / neural crest cell differentiation / NF-kappaB complex / rRNA modification in the nucleus and cytosol / negative regulation of phagocytosis / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / Formation of the ternary complex, and subsequently, the 43S complex / regulation of translational initiation / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / pigmentation / protein kinase A binding / negative regulation of ubiquitin protein ligase activity / Ribosomal scanning and start codon recognition / ion channel inhibitor activity / Translation initiation complex formation / phagocytic cup / positive regulation of mitochondrial depolarization / negative regulation of Wnt signaling pathway / positive regulation of T cell receptor signaling pathway / positive regulation of activated T cell proliferation / regulation of cell division / SARS-CoV-1 modulates host translation machinery / iron-sulfur cluster binding / Protein hydroxylation / TOR signaling / BH3 domain binding / mTORC1-mediated signalling / Peptide chain elongation / Selenocysteine synthesis / cysteine-type endopeptidase activator activity involved in apoptotic process / Formation of a pool of free 40S subunits / ribosomal small subunit export from nucleus / positive regulation of cyclic-nucleotide phosphodiesterase activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / translation regulator activity / SRP-dependent cotranslational protein targeting to membrane / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / gastrulation / spindle assembly / regulation of translational fidelity / MDM2/MDM4 family protein binding / laminin binding / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / rough endoplasmic reticulum / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Nuclear events stimulated by ALK signaling in cancer / negative regulation of smoothened signaling pathway / rescue of stalled ribosome / signaling adaptor activity
Similarity search - Function
Eukaryotic translation initiation factor 4B / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain ...Eukaryotic translation initiation factor 4B / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, C terminal / Ubiquitin-like protein FUBI / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / : / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S17e, conserved site / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein S19A/S15e / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S27, zinc-binding domain superfamily / 40S Ribosomal protein S10 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein S7e / Plectin/S10 domain / Ribosomal protein S8e / Ribosomal protein S4, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / RS4NT (NUC023) domain / Ribosomal protein S27 / Ribosomal S3Ae family / Ribosomal protein S17e signature. / Ribosomal protein S28e / Ribosomal family S4e / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S7e signature. / Ribosomal protein S6e / Ribosomal protein S3Ae signature. / Ribosomal protein S27e signature.
Similarity search - Domain/homology
Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Eukaryotic translation initiation factor 4B / Small ribosomal subunit protein eS12 / Eukaryotic translation initiation factor 2 subunit 3 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 ...Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Eukaryotic translation initiation factor 4B / Small ribosomal subunit protein eS12 / Eukaryotic translation initiation factor 2 subunit 3 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 3 subunit E / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS28 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein RACK1 / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit K / Eukaryotic translation initiation factor 3 subunit L
Similarity search - Component
Biological speciesHomo sapiens (human) / Human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.3 Å
AuthorsSchaffitzel C
CitationJournal: Nucleic Acids Res / Year: 2018
Title: Structure of a human cap-dependent 48S translation pre-initiation complex.
Authors: Boris Eliseev / Lahari Yeramala / Alexander Leitner / Manikandan Karuppasamy / Etienne Raimondeau / Karine Huard / Elena Alkalaeva / Ruedi Aebersold / Christiane Schaffitzel /
Abstract: Eukaryotic translation initiation is tightly regulated, requiring a set of conserved initiation factors (eIFs). Translation of a capped mRNA depends on the trimeric eIF4F complex and eIF4B to load ...Eukaryotic translation initiation is tightly regulated, requiring a set of conserved initiation factors (eIFs). Translation of a capped mRNA depends on the trimeric eIF4F complex and eIF4B to load the mRNA onto the 43S pre-initiation complex comprising 40S and initiation factors 1, 1A, 2, 3 and 5 as well as initiator-tRNA. Binding of the mRNA is followed by mRNA scanning in the 48S pre-initiation complex, until a start codon is recognised. Here, we use a reconstituted system to prepare human 48S complexes assembled on capped mRNA in the presence of eIF4B and eIF4F. The highly purified h-48S complexes are used for cross-linking/mass spectrometry, revealing the protein interaction network in this complex. We report the electron cryo-microscopy structure of the h-48S complex at 6.3 Å resolution. While the majority of eIF4B and eIF4F appear to be flexible with respect to the ribosome, additional density is detected at the entrance of the 40S mRNA channel which we attribute to the RNA-recognition motif of eIF4B. The eight core subunits of eIF3 are bound at the 40S solvent-exposed side, as well as the subunits eIF3d, eIF3b and eIF3i. elF2 and initiator-tRNA bound to the start codon are present at the 40S intersubunit side. This cryo-EM structure represents a molecular snap-shot revealing the h-48S complex following start codon recognition.
History
DepositionDec 31, 2017-
Header (metadata) releaseMar 14, 2018-
Map releaseMar 14, 2018-
UpdateOct 3, 2018-
Current statusOct 3, 2018Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6fec
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4242.png

Downloads & links

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Map

FileDownload / File: emd_4242.map.gz / Format: CCP4 / Size: 34.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationhuman 48S pre-initiation complex, reconstituted in the presence of capped mRNA, eIF4B and eIF4F, displaying eIF2 ternary complex, eIF3 and eIF4B
Voxel sizeX=Y=Z: 2.5 Å
Density
Contour LevelBy AUTHOR: 0.05 / Movie #1: 0.08
Minimum - Maximum-0.32222414 - 0.5438056
Average (Standard dev.)-0.000108246015 (±0.027071523)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions208208208
Spacing208208208
CellA=B=C: 520.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.52.52.5
M x/y/z208208208
origin x/y/z0.0000.0000.000
length x/y/z520.000520.000520.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS208208208
D min/max/mean-0.3220.544-0.000

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Supplemental data

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Sample components

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Entire : human cap-dependent 48S translation pre-initiation complex

EntireName: human cap-dependent 48S translation pre-initiation complex
Components
  • Complex: human cap-dependent 48S translation pre-initiation complex
    • Complex: human cap-dependent 48S translation pre-initiation complex
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit AEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit CEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit EEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit FEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit HEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit KEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit LEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit MEukaryotic initiation factor 3
      • Protein or peptide: EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT DEukaryotic initiation factor 3
      • RNA: 18S ribosomal RNA
      • Protein or peptide: 40S ribosomal protein S11
      • Protein or peptide: 40S ribosomal protein S16
      • Protein or peptide: 40S ribosomal protein S4, X isoformRibosome
      • Protein or peptide: 40S ribosomal protein S29
      • Protein or peptide: 40S ribosomal protein S9
      • Protein or peptide: 40S ribosomal protein S18
      • RNA: Transfer RNA (75-MER)
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 1
      • Protein or peptide: 40S ribosomal protein S23
      • Protein or peptide: 40S ribosomal protein S19
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 3
      • Protein or peptide: 40S ribosomal protein S5
      • Protein or peptide: 40S ribosomal protein S30
      • Protein or peptide: 40S ribosomal protein S25
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: 40S ribosomal protein S27
      • Protein or peptide: 40S ribosomal protein S13
      • Protein or peptide: 40S ribosomal protein S15a
      • Protein or peptide: 40S ribosomal protein S21
      • Protein or peptide: 40S ribosomal protein S2
      • Protein or peptide: EUKARYOTIC TRANSLATION INITIATION FACTOR 2 BETA SUBUNIT (eIF2-Beta)
      • Protein or peptide: 40S ribosomal protein S17
      • Protein or peptide: 40S ribosomal protein SA
      • Protein or peptide: 40S ribosomal protein S3
      • Protein or peptide: 40S ribosomal protein S20
      • Protein or peptide: 40S ribosomal protein S3a
      • Protein or peptide: 40S ribosomal protein S14
      • Protein or peptide: 40S ribosomal protein S26
      • Protein or peptide: 40S ribosomal protein S28
      • Protein or peptide: Receptor of activated protein C kinase 1
      • Protein or peptide: 40S ribosomal protein S15
      • Protein or peptide: 40S ribosomal protein S8
      • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
      • Protein or peptide: 40S ribosomal protein S6
      • Protein or peptide: 40S ribosomal protein S12
      • Protein or peptide: 40S ribosomal protein S24
      • Protein or peptide: 40S ribosomal protein S10
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit BEukaryotic initiation factor 3
    • Complex: mRNAMessenger RNA
      • RNA: Messenger RNA (26-MER)
    • Complex: Eukaryotic translation initiation factor 4B
      • Protein or peptide: Eukaryotic translation initiation factor 4B
  • Ligand: water

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Supramolecule #1: human cap-dependent 48S translation pre-initiation complex

SupramoleculeName: human cap-dependent 48S translation pre-initiation complex
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#50
Molecular weightTheoretical: 2.0 MDa

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Supramolecule #2: human cap-dependent 48S translation pre-initiation complex

SupramoleculeName: human cap-dependent 48S translation pre-initiation complex
type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#10, #12-#48, #50
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: mRNA

SupramoleculeName: mRNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #11
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #4: Eukaryotic translation initiation factor 4B

SupramoleculeName: Eukaryotic translation initiation factor 4B / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #49
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)

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Macromolecule #1: Eukaryotic translation initiation factor 3 subunit A

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 164.902656 KDa
SequenceString: MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKLAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS ...String:
MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKLAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS RVERLYHDIA QQAFKFCLQY TRKAEFRKLC DNLRMHLSQI QRHHNQSTAI NLNNPESQSM HLETRLVQLD SA ISMELWQ EAFKAVEDIH GLFSLSKKPP KPQLMANYYN KVSTVFWKSG NALFHASTLH RLYHLSREMR KNLTQDEMQR MST RVLLAT LSIPITPERT DIARLLDMDG IIVEKQRRLA TLLGLQAPPT RIGLINDMVR FNVLQYVVPE VKDLYNWLEV EFNP LKLCE RVTKVLNWVR EQPEKEPELQ QYVPQLQSNT ILRLLQQVAQ IYQSIEFSRL TSLVPFVDAF QLERAIVDAA RHCDL QVRI DHTSRTLSFG SDLNYATRED APIGPHLQSM PSEQIRNQLT AMSSVLAKAL EVIKPAHILQ EKEEQHQLAV TAYLKN SRK EHQRILARRQ TIEERKERLE SLNIQREKEE LEQREAELQK VRKAEEERLR QEAKEREKER ILQEHEQIKK KTVRERL EQ IKKTELGAKA FKDIDIEDLE ELDPDFIMAK QVEQLEKEKK ELQERLKNQE KKIDYFERAK RLEEIPLIKS AYEEQRIK D MDLWEQQEEE RITTMQLERE KALEHKNRMS RMLEDRDLFV MRLKAARQSV YEEKLKQFEE RLAEERHNRL EERKRQRKE ERRITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE LREYQERVKK LEEVERKKRQ RELEIEERER RREEERRLGE DPLSRRDSR WGDRDSEGTW RKGPEADSEW RRGPPEKEWR RGEGRDDERS HRRDEERPRR VGDDEDRESS LRPDDDRTLR R GMEDDRGP RRGPDEERFS RRGLEDERPS WRSTDDDRPP RRIGEEDRGS WRHAEDDRPP RRGLDDDRGS WRTADEDRGP RR MMDEDRG PRRGGADDER PSWRSADDDR GPRRGIDDDR GPRRGLDDDR GPWRNADDDR IPRRGADDDR GPWRNMDDDR MSR RADDDR ISRRGEDSRP GPWRPFVKPG GWREKEKARE ESWGPPRESR PPEEREWERE RDRDNPDREE NDKDPERERD RERD GDREE RFRRPRDEGG WRRGPAEEAS SWRDSSRRDD RDRDDRRRER DDRRDLRDRR DLRDDRDRRG PPLRSERDEV SSWRR ADDR KDDRAEERDP PRRVPPPVLS RDRDRDRDRD RDRDRERERE REREGEKEKT SWRAEKDRES LRRTKNETDE DGWTTV RR

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Macromolecule #2: Eukaryotic translation initiation factor 3 subunit C

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 97.923547 KDa
SequenceString: MKIRDVTKCL EEFELLGKAY GKAKSIVDKE GVPRFYIRIL ADLEDYLNEL WEDKEGKKKM NKNNAKALST LRQKIRKYNR DFESHITNY KQNPEQSADE DAEKNEEDSE GSSDEDEDED GVSAAAFLKK KAEAPSGESR KFLKKMEDED EDSEDSEDDE E WDTGSTSS ...String:
MKIRDVTKCL EEFELLGKAY GKAKSIVDKE GVPRFYIRIL ADLEDYLNEL WEDKEGKKKM NKNNAKALST LRQKIRKYNR DFESHITNY KQNPEQSADE DAEKNEEDSE GSSDEDEDED GVSAAAFLKK KAEAPSGESR KFLKKMEDED EDSEDSEDDE E WDTGSTSS DSDSEEEEGK QTALASRFLK KAPTTEEDKK AAEKKREDKA KKKHDRKSKR LDEEEEDNEG GEWERVRGGV PL VKEKPKM FAKGTEITHA VVIKKLNEIL QARGKKGTDR AAQIELLQLL VQIASENNLG EGVIVKIKFN IIASLYDYNP NLA TYMKPE MWQKCLDCIN ELMDILFANP NIFVGENILE ESENLQNVDQ PLRVRGCILT LVERMDEEFT KIMQNTDPHS QEYV EHLKD EAQVCAIIER VQRYLEEKGT TEEICRVYLR RILHTYYKFD YKAHQRQLTP PEGSSKSEQD QAENEGEDSA VLMER LCKY IYAKDRTDRI RTCAILCHIY HHYLHSRWYQ ARDLMLMSHL QDNIQHADPP VQILYNRTMV QLGICAFRQG LTKDAH NAL LDIQSSGRAK ELLGQGLLLR SLQERNQEQE KVERRRQVPF HLHINLELLE CVYLVSAMLL EIPYMAAHES DARRRMI SK QFHHQLRVGE RQPLLGPPES MREHVVAASK AMKMGDWKTC HSFIINEKMN GKVWDLFPEA DKVRTMLVRK IQEESLRT Y LFTYSSVYDS ISMETLSDMF ELDLPTVHSI ISKMIINEEL MASLDQPTQT VVMHRTEPTA QQNLALQLAE KLGSLVENN ERVFDHKQGT YGGYFRDQKD GYRKNEGYMR RGGYRQQQSQ TAY

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Macromolecule #3: Eukaryotic translation initiation factor 3 subunit E

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 52.281633 KDa
SequenceString: MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETE PIVKMFEDPE TTRQMQSTRD GRMLFDYLAD KHGFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP A TDRNALSS ...String:
MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETE PIVKMFEDPE TTRQMQSTRD GRMLFDYLAD KHGFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP A TDRNALSS LWGKLASEIL MQNWDAAMED LTRLKETIDN NSVSSPLQSL QQRTWLIHWS LFVFFNHPKG RDNIIDLFLY QP QYLNAIQ TMCPHILRYL TTAVITNKDV RKRRQVLKDL VKVIQQESYT YKDPITEFVE CLYVNFDFDG AQKKLRECES VLV NDFFLV ACLEDFIENA RLFIFETFCR IHQCISINML ADKLNMTPEE AERWIVNLIR NARLDAKIDS KLGHVVMGNN AVSP YQQVI EKTKSLSFRS QMLAMNIEKK LNQNSRSEAP NWATQDSGFY

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Macromolecule #4: Eukaryotic translation initiation factor 3 subunit F

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit F / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: ubiquitinyl hydrolase 1
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 37.84673 KDa
SequenceString: MATPAVSASA PPATPAATPA AAAAPASAPA AAPAPTPAPA PAAAPAAGAA PAAAADPAAA AAAAGTGQTP ASAQAPAQTS APSLPGPAL PGPFPGGRVV RLHPVILASI VDSYERRNEG AARVIGTLLG TVDKHSVEVT NCFSVPHNES EDEVAVDMEF A KNMYELHK ...String:
MATPAVSASA PPATPAATPA AAAAPASAPA AAPAPTPAPA PAAAPAAGAA PAAAADPAAA AAAAGTGQTP ASAQAPAQTS APSLPGPAL PGPFPGGRVV RLHPVILASI VDSYERRNEG AARVIGTLLG TVDKHSVEVT NCFSVPHNES EDEVAVDMEF A KNMYELHK KVSPNELILG WYATGHDITE HSVLIHEYYS REAPNPIHLT VDTSLQNGRM SIKAYVSTSM GVPGRTMGVM FT PLTVKYA YYDTERIGVD LIMKTCFSPN RVIGLSSDLQ QVGGASARIQ DALSTVLQYA EDVLSGKVSA DNTVGRFLMS LVN QVPKIV PDDFETMLNS NINDLLMVTY LANLTQSQIA LNEKLVNL

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Macromolecule #5: Eukaryotic translation initiation factor 3 subunit H

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit H / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 39.952281 KDa
SequenceString: MASRKEGTGS GGTSSSSATG VVGKGKGKGG SGDSAVKQVQ IDGLVVLKII KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ G SLSLKAYR ...String:
MASRKEGTGS GGTSSSSATG VVGKGKGKGG SGDSAVKQVQ IDGLVVLKII KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ G SLSLKAYR LTPKLMEVCK EKDFSPEALK KANITFEHMF EEVPIVIKNS HLINVLMWEL EKKSAVADKH ELLSLASSNH LG KNLQLLM DRVDEMSQDI VKYNTYMRNT SKQQQQKHQY QQRRQQENMQ RQSRGEPPLP EEDLSKLFKP PQPPARMDSL LIA GQINTY CQNIKEFTAQ NLGKLFMAQA LQEYNN

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Macromolecule #6: Eukaryotic translation initiation factor 3 subunit K

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit K / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 25.129709 KDa
SequenceString: MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLTDS ...String:
MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLTDS QLKMWMSKYG WSADESGQIF ICSQEESIKP KNIVEKIDFD SVSSIMASSQ

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Macromolecule #7: Eukaryotic translation initiation factor 3 subunit L

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit L / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 66.804766 KDa
SequenceString: MSYPADDYES EAVYDPYAYP SDYDMHTGDP KQDLAYERQY EQQTYQVIPE VIRNFIQYFH KTVSDLIDQK VYELQASRVS SDVIDQKVY EIQDIYENSW TKLTERFFKN TPWPEAETIA PQVGNDAVFL ILYKELYYRH IYAKVSGGPS LAQRFESYYN Y CNLFNYIL ...String:
MSYPADDYES EAVYDPYAYP SDYDMHTGDP KQDLAYERQY EQQTYQVIPE VIRNFIQYFH KTVSDLIDQK VYELQASRVS SDVIDQKVY EIQDIYENSW TKLTERFFKN TPWPEAETIA PQVGNDAVFL ILYKELYYRH IYAKVSGGPS LAQRFESYYN Y CNLFNYIL NADGPAPLEL PNQWLWDIID EFIYQFQSFS QYRCKTAKKS EEEIDFLRSN PKIWNVHSVL NVLHSLVDKS NI NRQLEVY TSGGDPESVA GEYGRHSLYK MLGYFSLVGL LRLHSLLGDY YQAIKVLENI ELNKKSMYSR VPECQVTTYY YVG FAYLMM RRYQDAIRVF ANILLYIQRT KSMFQRTTYK YEMINKQNEQ MHALLAIALT MYPMRIDESI HLQLREKYGD KMLR MQKGD PQVYEELFSY SCPKFLSPVV PNYDSVHPNY HKEPFLQQLK VFSDEVQQQA QLSTIRSFLK LYTTMPVAKL AGFLD LTEQ EFRIQLLVFK HKMKNLVWTS GISALDGEFQ SASEVDFYID KDMIHIADTK VARRYGDFFI RQIHKFEELN RTLKKM GQR P

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Macromolecule #8: Eukaryotic translation initiation factor 3 subunit M

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit M / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 42.555832 KDa
SequenceString: MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL ...String:
MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL YEALVDCKKS DAASKVMVEL LGSYTEDNAS QARVDAHRCI VRALKDPNAF LFDHLLTLKP VKFLEGELIH DL LTIFVSA KLASYVKFYQ NNKDFIDSLG LLHEQNMAKM RLLTFMGMAV ENKEISFDTM QQELQIGADD VEAFVIDAVR TKM VYCKID QTQRKVVVSH STHRTFGKQQ WQQLYDTLNA WKQNLNKVKN SLLSLSDT

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Macromolecule #9: EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT D

MacromoleculeName: EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT D / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 42.203555 KDa
SequenceString: GSSVTVRPDW VTIEEMDFPR LSKLTLPGVK EGEDVLCCGA VEYYDKSYDR VNVKNEKPLQ RIDRIFHTVT TTDDPVIRKL SKTEGNVYA TDAILATIMC CTRSNYSWDI VIEKIGNKLF FDKRDNTEFD LLTVNETSVE PPQDDGNSLN SPRNLALEAT F INHNFSQQ ...String:
GSSVTVRPDW VTIEEMDFPR LSKLTLPGVK EGEDVLCCGA VEYYDKSYDR VNVKNEKPLQ RIDRIFHTVT TTDDPVIRKL SKTEGNVYA TDAILATIMC CTRSNYSWDI VIEKIGNKLF FDKRDNTEFD LLTVNETSVE PPQDDGNSLN SPRNLALEAT F INHNFSQQ VLKSNEPRYK FDEPNPFISE EEEGEVASVA YRYRKWDLNN GITLIARCEH DAVMQGPNNE TQFLTIKALN EW DSKLANG VEWRRKLDTQ RGAVLANELR NNACKLAKWT VQALLAGSDQ LKFGYVSRAS VRDSSKHVIL ETQQYKPNEF ATQ INLNMD NAWGILRCII DICMNQKDGK YLIMKDPNKP MIRLYDIPDN TF

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Macromolecule #12: 40S ribosomal protein S11

MacromoleculeName: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 18.468826 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

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Macromolecule #13: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 15.975753 KDa
SequenceString:
PLQSVQVFGR KKTATAVAHC KRGNGLIKVN GRPLEMIEPR TLQYKLLEPV LLLGKERFAG VDIRVRVKGG GHVAQIYAIR QSISKALVA YYQKYVDEAS KKEIKDILIQ YDRTLLVADP RRCESKKFGG PGARARYQKS YR

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Macromolecule #14: 40S ribosomal protein S4, X isoform

MacromoleculeName: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 29.65892 KDa
SequenceString: MARGPKKHLK RVAAPKHWML DKLTSVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKMNDT I QIDLETGK ...String:
MARGPKKHLK RVAAPKHWML DKLTSVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKMNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

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Macromolecule #15: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 6.364426 KDa
SequenceString:
QQLYWSHPRK FGQGSRSCRV CSNRHGLIRK YGLNMCRQCF RQYAKDIGFI KLD

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Macromolecule #16: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 21.266166 KDa
SequenceString:
WVCRKTYVTP RRPFEKSRLD QELKLIGEYG LRNKREVWRV KFTLAKIRKA ARELLTLDEK DPRRLFEGNA LLRRLVRIGV LDEGKMKLD YILGLKIEDF LERRLQTQVF KLGLAKSIHH ARVLIRQRHI RVRKQVVNIP SFIVRLDSQK HIDFSLRSPY G GGRPGRVK RKNAKKGQGG AGAG

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Macromolecule #17: 40S ribosomal protein S18

MacromoleculeName: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 16.170774 KDa
SequenceString:
PEKFQHILRV LNTNIDGRRK IAFAITAIKG VGRRYAHVVL RKADIDLTKR AGELTEDEVE RVITIMQNPR QYKIPDWFLN RQKDVKDGK YSQVLANGLD NKLREDLERL KKIRAHRGLR HFWGLRVRGQ HTKTTGRR

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Macromolecule #19: Eukaryotic translation initiation factor 2 subunit 1

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 30.633297 KDa
SequenceString: CRFYQHKFPE VEDVVMVNVR SIAEMGAYVS LLEYNNIEGM ILLSELSRRR IRSINKLIRI GRNECVVVIR VDKEKGYIDL SKRRVSPEE AIKCEDKFTK SKTVYSILRH VAEVLEYTKD EQLESLFQRT AWVFDDKYKR PGYGAYDAFK HAVSDPSILD S LDLNEDER ...String:
CRFYQHKFPE VEDVVMVNVR SIAEMGAYVS LLEYNNIEGM ILLSELSRRR IRSINKLIRI GRNECVVVIR VDKEKGYIDL SKRRVSPEE AIKCEDKFTK SKTVYSILRH VAEVLEYTKD EQLESLFQRT AWVFDDKYKR PGYGAYDAFK HAVSDPSILD S LDLNEDER EVLINNINRR LTPQAVKIRA DIEVACYGYE GIDAVKEALR AGLNCSTETM PIKINLIAPP RYVMTTTTLE RT EGLSVLN QAMAVIKEKI EEKRGVFNV

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Macromolecule #20: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 15.757587 KDa
SequenceString:
MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPR

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Macromolecule #21: 40S ribosomal protein S19

MacromoleculeName: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 15.812286 KDa
SequenceString:
VTVKDVNQQE FVRALAAFLK KSGKLKVPEW VDTVKLAKHK ELAPYDENWF YTRAASTARH LYLRGGAGVG SMTKIYGGRQ RNGVMPSHF SRGSKSVARR VLQALEGLKM VEKDQDWGRK LTPQGQRDLD RIAGQVAAAK KK

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Macromolecule #22: Eukaryotic translation initiation factor 2 subunit 3

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit 3 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 45.862441 KDa
SequenceString: QATINIGTIG HVAHGKSTVV KAISGVHTVR FKNELERNIT IKLGYANAKI YKLDDPSCPR PECYRSCGSS TPDEFPTDIP GTKGNFKLV RHVSFVDCPG HDILMATMLN GAAVMDAALL LIAGNESCPQ PQTSEHLAAI EIMKLKHILI LQNKIDLVKE S QAKEQYEQ ...String:
QATINIGTIG HVAHGKSTVV KAISGVHTVR FKNELERNIT IKLGYANAKI YKLDDPSCPR PECYRSCGSS TPDEFPTDIP GTKGNFKLV RHVSFVDCPG HDILMATMLN GAAVMDAALL LIAGNESCPQ PQTSEHLAAI EIMKLKHILI LQNKIDLVKE S QAKEQYEQ ILAFVQGTVA EGAPIIPISA QLKYNIEVVC EYIVKKIPVP PRDFTSEPRL IVIRSFDVNK PGCEVDDLKG GV AGGSILK GVLKVGQEIE VRPGIVSKDS EGKLMCKPIF SKIVSLFAEH NDLQYAAPGG LIGVGTKIDP TLCRADRMVG QVL GAVGAL PEIFTELEIS YFLLRRLLGV RTEGDKKAAK VQKLSKNEVL MVNIGSLSTG GRVSAVKADL GKIVLTNPVC TEVG EKIAL SRRVEKHWRL IGWGQIR

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Macromolecule #23: 40S ribosomal protein S5

MacromoleculeName: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 21.525941 KDa
SequenceString:
TPDIKLFGKW STDDVQINDI SLQDYIAVKE KYAKYLPHSA GRYAAKRFRK AQCPIVERLT NSMMMHGRNN GKKLMTVRIV KHAFEIIHL LTGENPLQVL VNAIINSGPR EDSTRIGRAG TVRRQAVDVS PLRRVNQAIW LLCTGAREAA FRNIKTIAEC L ADELINAA KGSSNSYAIK KKDELERVAK SNR

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Macromolecule #24: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 6.741048 KDa
SequenceString:
KVHGSLARVG KVRGQTLKVA KQEKKKKRTG RAKRRMQYNR RFVNVVPTFG KKKGPNANS

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Macromolecule #25: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 8.526119 KDa
SequenceString:
RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKG

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Macromolecule #26: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 21.716387 KDa
SequenceString:
SAKIVKPNGE KPDEFESGIS QALLELEMNS DLKAQLRELN ITAAKEIEVG GGRKAIIIFV PVPQLKSFQK IQVRLVRELE KKFSGKHVV FIAQRRILPK PTRKSRTKNK QKRPRSRTLT AVHDAILEDL VFPSEIVGKR IRVKLDGSRL IKVHLDKAQQ N NVEHKVET FSGVYKKLTG KDVNFEFPEF QL

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Macromolecule #27: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

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Macromolecule #28: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 17.128191 KDa
SequenceString:
GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YESSTASALV A

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Macromolecule #29: 40S ribosomal protein S15a

MacromoleculeName: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 14.734357 KDa
SequenceString:
VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFDV QLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

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Macromolecule #30: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 8.896102 KDa
SequenceString:
MQSNAGEFVD LYVPRKCSAS NRIIGAKDHA SIPMNVAEVD KVTGRFNGQS KTYAICGAIR RMGESDDSIL RLAKSHGIVS QN

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Macromolecule #31: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 24.814285 KDa
SequenceString: GKAGDKEWLP VTKLGRLVKD MKIKSLEEIY LFSLPIKESE IIDFFLGAAL KDKVLKIMPV QKQTQAGQRT RFKAFVAIGD YNGHVGLGM KCSKEVATAI RGAIILAKLS IVPVRRGYWG NKIGKPHTVR CKVTGRCGSV LVRLIPAPRG TGIISAPVPK K LLLMAGID ...String:
GKAGDKEWLP VTKLGRLVKD MKIKSLEEIY LFSLPIKESE IIDFFLGAAL KDKVLKIMPV QKQTQAGQRT RFKAFVAIGD YNGHVGLGM KCSKEVATAI RGAIILAKLS IVPVRRGYWG NKIGKPHTVR CKVTGRCGSV LVRLIPAPRG TGIISAPVPK K LLLMAGID DCYTSAGGCT ATLGNFAKAT FDAISKTYSY LTPDLWKETV FTKSPYQEFT DHLVKTHT

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Macromolecule #32: EUKARYOTIC TRANSLATION INITIATION FACTOR 2 BETA SUBUNIT (eIF2-Beta)

MacromoleculeName: EUKARYOTIC TRANSLATION INITIATION FACTOR 2 BETA SUBUNIT (eIF2-Beta)
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 2.103416 KDa
SequenceString:
SEKEYVEMLD RLYSKLP

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Macromolecule #33: 40S ribosomal protein S17

MacromoleculeName: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 14.578988 KDa
SequenceString:
MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGM

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Macromolecule #34: 40S ribosomal protein SA

MacromoleculeName: 40S ribosomal protein SA / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 23.360791 KDa
SequenceString: SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA I PCNNKGAH ...String:
SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA I PCNNKGAH SVGLMWWMLA REVLRMRGTI SREHPWEVMP DLYFYRDPEE

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Macromolecule #35: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 25.158535 KDa
SequenceString: MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV ...String:
MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPSGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQK

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Macromolecule #36: 40S ribosomal protein S20

MacromoleculeName: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.76589 KDa
SequenceString:
AIHRIRITLT SRNVKSLEKV CADLIRGAKE KNLKVKGPVR MPTKTLRITT RKTPCGEGSK TWDRFQMRIH KRLIDLHSPS EIVKQITSI SIEPGVEVEV TIADA

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Macromolecule #37: 40S ribosomal protein S3a

MacromoleculeName: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 24.944408 KDa
SequenceString: KKVVDPFSKK DWYDVKAPAM FNIRNIGKTL VTRTQGTKIA SDGLKGRVFE VSLADLQNDE VAFRKFKLIT EDVQGKNCLT NFHGMDLTR DKMCSMVKKW QTMIEAHVDV KTTDGYLLHL FCVGFTKKCN NQIRKTSYAQ HQQVRQIRKK MMEIMTREVQ T NDLKEVVN ...String:
KKVVDPFSKK DWYDVKAPAM FNIRNIGKTL VTRTQGTKIA SDGLKGRVFE VSLADLQNDE VAFRKFKLIT EDVQGKNCLT NFHGMDLTR DKMCSMVKKW QTMIEAHVDV KTTDGYLLHL FCVGFTKKCN NQIRKTSYAQ HQQVRQIRKK MMEIMTREVQ T NDLKEVVN KLIPDSIGKD IEKACQSIYP LHDVFVRKVK MLKKPKFELG KLMELHG

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Macromolecule #38: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 14.544659 KDa
SequenceString:
SLGPQVAEGE NVFGVCHIFA SFNDTFVHVT DLSGKETICR VTGGMKVKAD RDESSPYAAM LAAQDVAQRC KELGITALHI KLRATGGNR TKTPGPGAQS ALRALARSGM KIGRIEDVTP IPSDSTRRKG GRRGRRL

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Macromolecule #39: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.315428 KDa
SequenceString:
TKKRRNNGRA KKGRGHVQPI RCTNCACCVP KDKAIKKFVI RNIVEAAAVR DISEVSVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPPPR

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Macromolecule #40: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 7.263394 KDa
SequenceString:
RVQPIKLARV TKVLGRTGSQ GQCTQVRVEF MDDTSRSIIR NVKGPVREGD VLTLLESERE ARRL

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Macromolecule #41: Receptor of activated protein C kinase 1

MacromoleculeName: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 34.669113 KDa
SequenceString: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG ...String:
TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTI

+
Macromolecule #42: 40S ribosomal protein S15

MacromoleculeName: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 15.151948 KDa
SequenceString:
VEQKKKRTFR KFTYRGVDLD QLLDMSYEQL MQLYSARQRR RLSRGLRRKQ HSLLKRLRKA KKEAPPMEKP EVVKTHLRDM IILPEMVGS MVGVYNGKTF NQVEIKPEMI GHYLGEFSIT YKPVKHGR

+
Macromolecule #43: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 23.902871 KDa
SequenceString: GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVGVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG ...String:
GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVGVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARKG

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Macromolecule #44: Ubiquitin-40S ribosomal protein S27a

MacromoleculeName: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 8.358903 KDa
SequenceString:
KKSYTTPKKN KHKRKKVKLA VLKYYKVDEN GKISRLRREC PSDECGAGVF MASHFDRHYC GKCCLTYCFN K

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Macromolecule #45: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 27.471535 KDa
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSL

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Macromolecule #46: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 13.766122 KDa
SequenceString:
GGVMDVNTAL QEVLKTALIH DGLARGIREA AKALDKRQAH LCVLASNCDE PMYVKLVEAL CAEHQINLIK VDDNKKLGEW VGLCKIDRE GKPRKVVGCS CVVVKDYGKE SQAKDVIEEY FKCKK

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Macromolecule #47: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 15.18897 KDa
SequenceString:
DTVTIRTRKF MTNRLLQRKQ MVIDVLHPGK ATVPKTEIQE KLAKMYKTTP DVIFVFGFRT HFGGGKTTGF GMIYDSLDYA KKNEPKHRL ARHGLYEKKK TSRKQRKERK NRMKKVRGTA KANVGAGKKP KE

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Macromolecule #48: 40S ribosomal protein S10

MacromoleculeName: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.773953 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSR

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Macromolecule #49: Eukaryotic translation initiation factor 4B

MacromoleculeName: Eukaryotic translation initiation factor 4B / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 72.32482 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MSYYHHHHHH DYDIPTTENL YPQGAMAASA KKKNKKGKTI SLTDFLAEDG GTGGGSTYVS KPVSWADETD DLEGDVSTTW HSNDDDVYR APPIDRSILP TAPRAAREPN IDRSRLPKSP PYTAFLGNLP YDVTEESIKE FFRGLNISAV RLPREPSNPE R LKGFGYAE ...String:
MSYYHHHHHH DYDIPTTENL YPQGAMAASA KKKNKKGKTI SLTDFLAEDG GTGGGSTYVS KPVSWADETD DLEGDVSTTW HSNDDDVYR APPIDRSILP TAPRAAREPN IDRSRLPKSP PYTAFLGNLP YDVTEESIKE FFRGLNISAV RLPREPSNPE R LKGFGYAE FEDLDSLLSA LSLNEESLGN RRIRVDVADQ AQDKDRDDRS FGRDRNRDSD KTDTDWRARP ATDSFDDYPP RR GDDSFGD KYRDRYDSDR YRDGYRDGYR DGPRRDMDRY GGRDRYDDRG SRDYDRGYDS RIGSGRRAFG SGYRRDDDYR GGG DRYEDR YDRRDDRSWS SRDDYSRDDY RRDDRGPPQR PKLNLKPRST PKEDDSSAST SQSTRAASIF GGAKPVDTAA RERE VEERL QKEQEKLQRQ LDEPKLERRP RERHPSWRSE ETQERERSRT GSESSQTGTS TTSSRNARRR ESEKSLENET LNKEE DCHS PTSKPPKPDQ PLKVMPAPPP KENAWVKRSS NPPARSQSSD TEQQSPTSGG GKVAPAQPSE EGPGRKDENK VDGMNA PKG QTGNSSRGPG DGGNRDHWKE SDRKDGKKDQ DSRSAPEPKK PEENPASKFS SASKYAALSV DGEDENEGED YAE

+
Macromolecule #50: Eukaryotic translation initiation factor 3 subunit B

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 124.402336 KDa
SequenceString: MLGAVKMEGH ESSDWNSYYA DTQEAYSSVP VSNMNSGLGS MNSMNTYMTM NTMTTSGNMT PASFNMSYAN PGLGAGLSPG AVAGMPAGS AGAMNSMTAA GVTAMGTALS PGGMGAMGAQ PAASMNGLGP YAAAMNPCMS PMAYAPSNLG RSRAGGGGDA K TFKRSYPH ...String:
MLGAVKMEGH ESSDWNSYYA DTQEAYSSVP VSNMNSGLGS MNSMNTYMTM NTMTTSGNMT PASFNMSYAN PGLGAGLSPG AVAGMPAGS AGAMNSMTAA GVTAMGTALS PGGMGAMGAQ PAASMNGLGP YAAAMNPCMS PMAYAPSNLG RSRAGGGGDA K TFKRSYPH AKPPYSYISL ITMAIQQAPS KMLTLSEIYQ WIMDLFPYYR QNQQRWQNSI RHSLSFNDCF LKVPRSPDKP GK GSFWTLH PDSGNMFENG CYLRRQKRFK CEKQLALKEA GAPGAGAGGK KAAAVGAQAS QAQLGEVAGP APDTPAGTES PHS SASPCQ EHKRVALGEL KGSAAGAALS PPEPAPSPGQ QQQQQPGAEP PPEEEPPRPA GAEDAAAEAA AEAGPEPDVR AEPA EAQEA ASSPAEPPAT LPAEQGEAPR EQARDEPAPA SEAEGSAAEA EPRAVENGDA DEPSFSDPED FVDDVSEEEL LGDVL KDRP QEADGIDSVI VVDNVPQVGP DRLEKLKNVI HKIFSKFGKI TNDFYPEEDG KTKGYIFLEY ASPAHAVDAV KNADGY KLD KQHTFRVNLF TDFDKYMTIS DEWDIPEKQP FKDLGNLRYW LEEAECRDQY SVIFESGDRT SIFWNDVKDP VSIEERA RW TETYVRWSPK GTYLATFHQR GIALWGGEKF KQIQRFSHQG VQLIDFSPCE RYLVTFSPLM DTQDDPQAII IWDILTGH K KRGFHCESSA HWPIFKWSHD GKFFARMTLD TLSIYETPSM GLLDKKSLKI SGIKDFSWSP GGNIIAFWVP EDKDIPARV TLMQLPSRQE IRVRNLFNVV DCKLHWQKNG DYLCVKVDRT PKGTQGVVTN FEIFRMREKQ VPVDVVEMKE TIIAFAWEPN GSKFAVLHG EAPRISVSFY HVKSNGKIEL SKMFDKQQAN TIFWSPQGQF VVLAGLRSMN GALAFVDTSD CTVMNIAEHY M ASDVEWDP TGRYVVTSVS WWSHKVDNAY WLWTFQGRLL QKNNKDRFCQ LLWRPRPPTL LSQEQIKQIK KDLKKYSKIF EQ KDRLSQS KASKELVERR RAMMEDFRKY GKAAQELYVG QKNERLELRG GVDTDELDSN VDDWEEETIE FFVTEEVIPL GSQ E

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Macromolecule #10: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 10 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 572.789812 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGCGCU GACUCCCUUU GUGUGGGAUG CGUGCAUUUA UCAGAUCAAA ACCAACCCGG UC AGCGGCG GCGGCUUUGG UGACUCUAGA UAACCUCGGG CCGAUCGCAG CCCUCCGUGG CGGCGACGAC CCAUUCGAAC GUC UGCCCU AUCAACUUUC GAUGGUAGUC GCCGUGCCUA CCAUGGUGAC CACGGGUGAC GGGGAAUCAG GGUUCGAUUC CGGA GAGGG AGCCUGAGAA ACGGCUACCA CAUCCAAGGA AGGCAGCAGG CGCGCAAAUU ACCCACUCCC GACCCGGGGA GGUAG UGAC GAAAAAUAAC AAUACAGGAC UCUUUCGAGG CCCUGUAAUU GGAAUGAGUC CACUUUAAAU CCUUUAACGA GGAUCC AUU GGAGGGCAAG UCUGGUCGCA GCAGCCGCGG UAAUUCCAGC UCCAAUAGCG UAUAUUAAAG UUGCUGCAGU UAAAAAG CU CGUAGUUGGA UCUUGAGGGC CCGCCCCUCG GCGCCCCCUC GAUGUGUCCC GCGGGGCCCG AAGCGUUUAC UUUGAAAA A AUUAGAGUGU UCAAAGCAGG CCCGAGCCGC CUGGAUACCG CAGCUAGGAA UAAUGGAAUA GGACCGCGGU UCUAUUUUG UUGGUUUUCG GAACUGAGGC CAUGAUUAAG AGGGACGGCC GGGGGCAUUC GUAUUGCGCC GCUAGAGGUG AAAUUCUUGG ACCGGCGCA AGACGGACCA GAGCGAAAGC AUUUGCCAAG AAUGUUUUCA UUAAUCAAGA ACGAAAGUCG GAGGUUCGAA G ACGAUCAG AUACCGUCGU AGUUCCGACC AUAAACGAUG CCGACUGGCG AUGCGGCGGC GUUAUUCCCA UGACCCGCCG GG CAGCUUC CGGGAAACCA AAGUCUUUGG GUUCCGGGGG GAGUAUGGUU GCAAAGCUGA AACUUAAAGG AAUUGACGGA AGG GCACCA CCAGGAGUGG AGCCUGCGGC UUAAUUUGAC UCAACACGGG AAACCUCACC CGGCCCGGAC ACGGACAGGA UUGA CAGAU UGAUAGCUCU UUCUCGAUUC UGUGGGUGGU GGUGCAUGGC CGUUCUUAGU UGGUGGAGCG AUUUGUCUGG UUAAU UCCG AUAACGAACG AGACUCUGGC AUGCUAACUA GUUACGCGAC CCCCGAGCGG UCGGCGUCCC CCAACUUCUU AGAGGG ACA AGUGGCGUUC AGCCACCCGA GAUUGAGCAA UAACAGGUCU GUGAUGCCCU UAGAUGUCGG GGCUGCACGC GCGCUAC AC UGACUGGCUC AGCGUGUGCC UACCCUACGC CGGCAGGCGC GGGUAACCCG UUGAACCCCA UUCGUGAUGG GGAUCGGG G AUUGCAAUUA UUCCCCAUGA ACGAGGAAUU CCCAGUAAGU GCGGGUCAUA AGCUUGCGUU GAUUAAGUCC CUGCCCUUU GUACACACCG CCCGUCGCUA CUACCGAUUG GAUGGUUUAG UGAGGCCCUC GGAUCGGCCC GCCGGGGUCG GCCCACGGCC CUGGCGGAG CGCUGAGAAG ACGGUCGAAC UUGACUAUCU AGAGGAAGUA AAAGUCGUAA CAAGGUUUCC GUAGGUGAAC C UGCGGAAG GAUCAUUA

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Macromolecule #11: Messenger RNA (26-MER)

MacromoleculeName: Messenger RNA (26-MER) / type: rna / ID: 11 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 8.238953 KDa
SequenceString:
CAGACACCAU GGUGCACCUG ACUCCU

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Macromolecule #18: Transfer RNA (75-MER)

MacromoleculeName: Transfer RNA (75-MER) / type: rna / ID: 18 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.23151 KDa
SequenceString:
AGCAGAGUGG CGCAGCGGAA GCGUGCUGGG CCCAUAACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

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Macromolecule #51: water

MacromoleculeName: water / type: ligand / ID: 51 / Number of copies: 415 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Details: 20 mM Tris HCl, 50 mM KOAc, 2.5 mM MgCl2, 2 mM DTT, 0.25 mM spermidine 0.25 mM GMPPNP
GridModel: Quantifoil R2/2
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 112000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 30.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: EMDB MAP
EMDB ID:

3019

Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 1.4)
Final 3D classificationSoftware - Name: RELION (ver. 1.4)
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 1.4)
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 6.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 50604

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT / Target criteria: Cross-correlation coefficient
Output model

PDB-6fec:
Human cap-dependent 48S pre-initiation complex

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