[English] 日本語
Yorodumi
- EMDB-4050: 70S ribosome from Staphylococcus aureus -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-4050
Title70S ribosome from Staphylococcus aureusRibosome
Map data
Sample
  • Complex: 70S ribosome from Staphylococcus aureusRibosome
    • RNA: x 3 types
    • Protein or peptide: x 47 types
  • Ligand: x 4 types
Function / homology
Function and homology information


ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / cytoplasmic translation / cytosolic large ribosomal subunit ...ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / cytoplasmic translation / cytosolic large ribosomal subunit / transferase activity / negative regulation of translation / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 ...Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S6, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein S16 domain superfamily / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein S15, bacterial-type / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein S2 signature 2. / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein
Similarity search - Domain/homology
Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 ...Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL33B / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10
Similarity search - Component
Biological speciesStaphylococcus aureus (strain NCTC 8325) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsKhusainov I / Vicens Q / Bochler A / Grosse F / Myasnikov A / Menetret JF / Chicher J / Marzi S / Romby P / Yusupova G ...Khusainov I / Vicens Q / Bochler A / Grosse F / Myasnikov A / Menetret JF / Chicher J / Marzi S / Romby P / Yusupova G / Yusupov M / Hashem Y
CitationJournal: Nucleic Acids Res / Year: 2016
Title: Structure of the 70S ribosome from human pathogen Staphylococcus aureus.
Authors: Iskander Khusainov / Quentin Vicens / Anthony Bochler / François Grosse / Alexander Myasnikov / Jean-François Ménétret / Johana Chicher / Stefano Marzi / Pascale Romby / Gulnara Yusupova ...Authors: Iskander Khusainov / Quentin Vicens / Anthony Bochler / François Grosse / Alexander Myasnikov / Jean-François Ménétret / Johana Chicher / Stefano Marzi / Pascale Romby / Gulnara Yusupova / Marat Yusupov / Yaser Hashem /
Abstract: Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact ...Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely related organisms, their existence could account for vital yet species-specific mechanisms of translation regulation that would involve stalling, cell survival and antibiotic resistance. Here, we present the first full 70S ribosome structure from Staphylococcus aureus, a Gram-positive pathogenic bacterium, solved by cryo-electron microscopy. Comparative analysis with other known bacterial ribosomes pinpoints several unique features specific to S. aureus around a conserved core, at both the protein and the RNA levels. Our work provides the structural basis for the many studies aiming at understanding translation regulation in S. aureus and for designing drugs against this often multi-resistant pathogen.
History
DepositionJul 13, 2016-
Header (metadata) releaseDec 28, 2016-
Map releaseDec 28, 2016-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

-
Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.085
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.085
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-5li0
  • Surface level: 0.085
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4050.jpg

emd_4050.png

Downloads & links

-
Map

FileDownload / File: emd_4050.map.gz / Format: CCP4 / Size: 139.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.1 Å
Density
Contour LevelBy AUTHOR: 0.095 / Movie #1: 0.085
Minimum - Maximum-0.23831725 - 0.44680446
Average (Standard dev.)0.0016471746 (±0.02667443)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions332332332
Spacing332332332
CellA=B=C: 365.2 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.11.11.1
M x/y/z332332332
origin x/y/z0.0230.0230.023
length x/y/z365.200365.200365.200
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS332332332
D min/max/mean-0.2380.4470.002

-
Supplemental data

-
Sample components

+
Entire : 70S ribosome from Staphylococcus aureus

EntireName: 70S ribosome from Staphylococcus aureusRibosome
Components
  • Complex: 70S ribosome from Staphylococcus aureusRibosome
    • RNA: 16S ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31 type BRibosome
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 2Ribosome
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
  • Ligand: MAGNESIUM ION
  • Ligand: OXYGEN ATOMOxygen
  • Ligand: ZINC ION
  • Ligand: water

+
Supramolecule #1: 70S ribosome from Staphylococcus aureus

SupramoleculeName: 70S ribosome from Staphylococcus aureus / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#50
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)

+
Macromolecule #1: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 500.464406 KDa
SequenceString: GGAGAGUUUG AUCCUGGCUC AGGAUGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGAACGGAC GAGAAGCUUG CUUCUCUGA UGUUAGCGGC GGACGGGUGA GUAACACGUG GAUAACCUAC CUAUAAGACU GGGAUAACUU CGGGAAACCG U AGCUAAUA ...String:
GGAGAGUUUG AUCCUGGCUC AGGAUGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGAACGGAC GAGAAGCUUG CUUCUCUGA UGUUAGCGGC GGACGGGUGA GUAACACGUG GAUAACCUAC CUAUAAGACU GGGAUAACUU CGGGAAACCG U AGCUAAUA CCGGAUAAUA UUUUGAACCG CAUGGUUCAA AAGUGAAAGA CGGUCUUGCU GUCACUUAUA GAUGGAUCCG CG CUGCAUU AGCUAGUUGG UAAGGUAACG GCUUACCAAG GCAACGAUGC AUAGCCGACC UGAGAGGGUG AUCGGCCACA CUG GAACUG AGACACGGUC CAGACUCCUA CGGGAGGCAG CAGUAGGGAA UCUUCCGCAA UGGGCGAAAG CCUGACGGAG CAAC GCCGC GUGAGUGAUG AAGGUCUUCG GAUCGUAAAA CUCUGUUAUU AGGGAAGAAC AUAUGUGUAA GUAACUGUGC ACAUC UUGA CGGUACCUAA UCAGAAAGCC ACGGCUAACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGU GGCAAGCGUU AUCCGG AAU UAUUGGGCGU AAAGCGCGCG UAGGCGGUUU UUUAAGUCUG AUGUGAAAGC CCACGGCUCA ACCGUGGAGG GUCAUUG GA AACUGGAAAA CUUGAGUGCA GAAGAGGAAA GUGGAAUUCC AUGUGUAGCG GUGAAAUGCG CAGAGAUAUG GAGGAACA C CAGUGGCGAA GGCGACUUUC UGGUCUGUAA CUGACGCUGA UGUGCGAAAG CGUGGGGAUC AAACAGGAUU AGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUAGGG GGUUUCCCGC CCCUUAGUGC UGCAGCUAAC GCAUUAAGCA CUCCGCCUG GGGAGUACGA CCGCAAGGUU GAAACUCAAA GGAAUUGACG GGGACCCGCA CAAGCGGUGG AGCAUGUGGU U UAAUUCGA AGCAACGCGA AGAACCUUAC CAAAUCUUGA CAUCCUUUGA CAACUCUAGA GAUAGAGCCU UCCCCUUCGG GG GACAAAG UGACAGGUGG UGCAUGGUUG UCGUCAGCUC GUGUCGUGAG AUGUUGGGUU AAGUCCCGCA ACGAGCGCAA CCC UUAAGC UUAGUUGCCA UCAUUAAGUU GGGCACUCUA AGUUGACUGC CGGUGACAAA CCGGAGGAAG GUGGGGAUGA CGUC AAAUC AUCAUGCCCC UUAUGAUUUG GGCUACACAC GUGCUACAAU GGACAAUACA AAGGGCAGCG AAACCGCGAG GUCAA GCAA AUCCCAUAAA GUUGUUCUCA GUUCGGAUUG UAGUCUGCAA CUCGACUACA UGAAGCUGGA AUCGCUAGUA AUCGUA GAU CAGCAUGCUA CGGUGAAUAC GUUCCCGGGU CUUGUACACA CCGCCCGUCA CACCACGAGA GUUUGUAACA CCCGAAG CC GGUGGAGUAA CCUUUUAGGA GCUAGCCGUC GAAGGUGGGA CAAAUGAUUG GGGUGAAGUC GUAACAAGGU AGCCGUAU C GGAAGGUGCG GCUGGAUCAC CUCCUU

+
Macromolecule #21: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 21 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 945.058562 KDa
SequenceString: UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC ...String:
UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC AUAUCAGAAG GCACACCCGG AGAACUGAAA CAUCUUAGUA CCCGGAGGAA GAGAAAGAAA AUUCGAUUCC CU UAGUAGC GGCGAGCGAA AUGGGAAGAG CCCAAACCAA CAAGCUUGCU UGUUGGGGUU GUAGGACACU CUAUACGGAG UUA CAAAGG ACGACAUUAG ACGAAUCAUC UGGAAAGAUG AAUCAAAGAA GGUAAUAAUC CUGUAGUCGA AAAUGUUGUC UCUC UUGAG UGGAUCCUGA GUACGACGGA GCACGUGAAA UUCCGUCGGA AUCUGGGAGG ACCAUCUCCU AAGGCUAAAU ACUCU CUAG UGACCGAUAG UGAACCAGUA CCGUGAGGGA AAGGUGAAAA GCACCCCGGA AGGGGAGUGA AAUAGAACCU GAAACC GUG UGCUUACAAG UAGUCAGAGC CCGUUAAUGG GUGAUGGCGU GCCUUUUGUA GAAUGAACCG GCGAGUUACG AUUUGAU GC AAGGUUAAGC AGUAAAUGUG GAGCCGUAGC GAAAGCGAGU CUGAAUAGGG CGUUUAGUAU UUGGUCGUAG ACCCGAAA C CAGGUGAUCU ACCCUUGGUC AGGUUGAAGU UCAGGUAACA CUGAAUGGAG GACCGAACCG ACUUACGUUG AAAAGUGAG CGGAUGAACU GAGGGUAGCG GAGAAAUUCC AAUCGAACCU GGAGAUAGCU GGUUCUCUCC GAAAUAGCUU UAGGGCUAGC CUCAAGUGA UGAUUAUUGG AGGUAGAGCA CUGUUUGGAC GAGGGGCCCC UCUCGGGUUA CCGAAUUCAG ACAAACUCCG A AUGCCAAU UAAUUUAACU UGGGAGUCAG AACAUGGGUG AUAAGGUCCG UGUUCGAAAG GGAAACAGCC CAGACCACCA GC UAAGGUC CCAAAAUAUA UGUUAAGUGG AAAAGGAUGU GGCGUUGCCC AGACAACUAG GAUGUUGGCU UAGAAGCAGC CAU CAUUUA AAGAGUGCGU AAUAGCUCAC UAGUCGAGUG ACACUGCGCC GAAAAUGUAC CGGGGCUAAA CAUAUUACCG AAGC UGUGG AUUGUCCUUU GGACAAUGGU AGGAGAGCGU UCUAAGGGCG UUGAAGCAUG AUCGUAAGGA CAUGUGGAGC GCUUA GAAG UGAGAAUGCC GGUGUGAGUC GCGAAAGACG GGUGAGAAUC CCGUCCACCG AUUGACUAAG GUUUCCAGAG GAAGGC UCG UCCGCUCUGG GUUAGUCGGG UCCUAAGCUG AGGCCGACAG GCGUAGGCGA UGGAUAACAG GUUGAUAUUC CUGUACC AC CUAUAAUCGU UUUAAUCGAU GGGGGGACGC AGUAGGAUAG GCGAAGCGUG CGAUUGGAUU GCACGUCUAA GCAGUAAG G CUGAGUAUUA GGCAAAUCCG GUACUCGUUA AGGCUGAGCU GUGAUGGGGA GAAGACAUUG AGUCUUCGAG UCGUUGAUU UCACACUGCC GAGAAAAGCC UCUAGAUAGA AAAUAGGUGC CCGUACCGCA AACCGACACA GGUAGUCAAG AUGAGAAUUC UAAGGUGAG CGAGCGAACU CUCGUUAAGG AACUCGGCAA AAUGACCCCG UAACUUCGGG AGAAGGGGUG CUCUUUAGGG U UAACGCCC AGAAGAGCCG CAGUGAAUAG GCCCAAGCGA CUGUUUAUCA AAAACACAGG UCUCUGCUAA ACCGUAAGGU GA UGUAUAG GGGCUGACGC CUGCCCGGUG CUGGAAGGUU AAGAGGAGUG GUUAGCUUCU GCGAAGCUAC GAAUCGAAGC CCC AGUAAA CGGCGGCCGU AACUAUAACG GUCCUAAGGU AGCGAAAUUC CUUGUCGGGU AAGUUCCGAC CCGCACGAAA GGCG UAACG AUUUGGGCAC UGUCUCAACG AGAGACUCGG UGAAAUCAUA GUACCUGUGA AGAUGCAGGU UACCCGCGAC AGGAC GGAA AGACCCCGUG GAGCUUUACU GUAGCCUGAU AUUGAAAUUC GGCACAGCUU GUACAGGAUA GGUAGGAGCC UUUGAA ACG UGAGCGCUAG CUUACGUGGA GGCGCUGGUG GGAUACUACC CUAGCUGUGU UGGCUUUCUA ACCCGCACCA CUUAUCG UG GUGGGAGACA GUGUCAGGCG GGCAGUUUGA CUGGGGCGGU CGCCUCCUAA AAGGUAACGG AGGCGCUCAA AGGUUCCC U CAGAAUGGUU GGAAAUCAUU CAUAGAGUGU AAAGGCAUAA GGGAGCUUGA CUGCGAGACC UACAAGUCGA GCAGGGUCG AAAGACGGAC UUAGUGAUCC GGUGGUUCCG CAUGGAAGGG CCAUCGCUCA ACGGAUAAAA GCUACCCCGG GGAUAACAGG CUUAUCUCC CCCAAGAGUU CACAUCGACG GGGAGGUUUG GCACCUCGAU GUCGGCUCAU CGCAUCCUGG GGCUGUAGUC G GUCCCAAG GGUUGGGCUG UUCGCCCAUU AAAGCGGUAC GCGAGCUGGG UUCAGAACGU CGUGAGACAG UUCGGUCCCU AU CCGUCGU GGGCGUAGGA AAUUUGAGAG GAGCUGUCCU UAGUACGAGA GGACCGGGAU GGACAUACCU CUGGUGUACC AGU UGUCGU GCCAACGGCA UAGCUGGGUA GCUAUGUGUG GACGGGAUAA GUGCUGAAAG CAUCUAAGCA UGAAGCCCCC CUCA AGAUG AGAUUUCCCA ACUUCGGUUA UAAGAUCCCU CAAAGAUGAU GAGGUUAAUA GGUUCGAGGU GGAAGCAUGG UGACA UGUG GAGCUGACGA AUACUAAUCG AUCGAAGACU UAAU

+
Macromolecule #22: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 22 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 36.692801 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGGUC UUUAGCGACG AUGGUAGCCA ACUUACGUU CCGCUAGAGU AGAACGUUGC CAGGC

+
Macromolecule #2: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 29.136369 KDa
SequenceString: MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ...String:
MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ERNAIAEARK LNIPIVGIVD TNCDPDEIDY VIPANDDAIR AVKLLTAKMA DAILEGQQGV SNEEVAAEQN ID LDEKEKS EETEATEE

+
Macromolecule #3: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 24.143867 KDa
SequenceString: MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS ...String:
MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS EGTVPLHTLR ADIDYAHAEA DTTYGKLGVK VWIYRGEVLP TKNTSGGGK

+
Macromolecule #4: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 22.920221 KDa
SequenceString: ARFRGSNWKK SRRLGISLSG TGKELEKRPY APGQHGPNQR KKLSEYGLQL REKQKLRYLY GMTERQFRNT FDIAGKKFGV HGENFMILL ASRLDAVVYS LGLARTRRQA RQLVNHGHIL VDGKRVDIPS YSVKPGQTIS VREKSQKLNI IVESVEINNF V PEYLNFDA ...String:
ARFRGSNWKK SRRLGISLSG TGKELEKRPY APGQHGPNQR KKLSEYGLQL REKQKLRYLY GMTERQFRNT FDIAGKKFGV HGENFMILL ASRLDAVVYS LGLARTRRQA RQLVNHGHIL VDGKRVDIPS YSVKPGQTIS VREKSQKLNI IVESVEINNF V PEYLNFDA DSLTGTFVRL PERSELPAEI NEQLIVEYYS R

+
Macromolecule #5: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 17.639314 KDa
SequenceString:
ARREEETKEF EERVVTINRV AKVVKGGRRF RFTALVVVGD KNGRVGFGTG KAQEVPEAIK KAVEAAKKDL VVVPRVEGTT PHTITGRYG SGSVFMKPAA PGTGVIAGGP VRAVLELAGI TDILSKSLGS NTPINMVRAT IDGLQNLKNA EDVAKLRGKT V EELYN

+
Macromolecule #6: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 11.368879 KDa
SequenceString:
MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIREDE

+
Macromolecule #7: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 17.167736 KDa
SequenceString:
VPKRDVLPDP IHNSKLVTKL INKIMLDGKR GTAQRILYSA FDLVEQRSGR DALEVFEEAI NNIMPVLEVK ARRVGGSNYQ VPVEVRPER RTTLGLRWLV NYARLRGEKT MEDRLANEIL DAANNTGGAV KKREDTHKMA EANKAFAHYR W

+
Macromolecule #8: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 14.723118 KDa
SequenceString:
TMTDPIADML TRVRNANMVR HEKLELPASN IKKEIAEILK SEGFIKNVEY VEDDKQGVLR LFLKYGQNDE RVITGLKRIS KPGLRVYAK ASEMPKVLNG LGIALVSTSE GVITDKEARK RNVGGEIIAY VW

+
Macromolecule #9: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 14.44045 KDa
SequenceString:
QVEYRGTGRR KNSVARVRLV PGEGNITVNN RDVREYLPFE SLILDLNQPF DVTETKGNYD VLVNVHGGGF TGQAQAIRHG IARALLEAD PEYRGSLKRA GLLTRDPRMK ERKKPGLKAA RRSPQFSKR

+
Macromolecule #10: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 11.60052 KDa
SequenceString:
MAKQKIRIRL KAYDHRVIDQ SAEKIVETAK RSGADVSGPI PLPTEKSVYT IIRAVHMYKD SREQFEQRTH KRLIDIVNPT PKTVDALMG LNLPSGVDIE IKL

+
Macromolecule #11: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 12.562294 KDa
SequenceString:
RRVKKNIENG VAHIRSTFNN TIVTITDEFG NALSWSSAGA LGFKGSKKST PFAAQMASET ASKSAMEHGL KTVEVTVKGP GPGRESAIR ALQSAGLEVT AIRDVTPVPH NGCRPPKRR

+
Macromolecule #12: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 15.131598 KDa
SequenceString:
PTINQLVRKP RQSKIKKSDS PALNKGFNSK KKKFTDLNSP QKRGVCTRVG TMTPRKPNSA LRKYARVRLS NNIEINAYIP GIGHNLQEH SVVLVRGGRV RDLPGVRYHI VRGALDTSGV DGRRQGRSLY GTKKPK

+
Macromolecule #13: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 13.487543 KDa
SequenceString:
ARIAGVDIPR EKRVVISLTY IYGIGTSTAQ KILEEANVSA DTRVKDLTDD ELGRIREVVD GYKVEGDLRR ETNLNIKRLM EISSYRGIR HRRGLPVRGQ KTKNNARTRK GPVKTVANKK

+
Macromolecule #14: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 7.186573 KDa
SequenceString:
AKTSMVAKQQ KKQKYAVREY TRCERCGRPH SVYRKFKLCR ICFRELAYKG QIPGVRKASW

+
Macromolecule #15: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 10.503135 KDa
SequenceString:
AISQERKNEI IKEYRVHETD TGSPEVQIAV LTAEINAVNE HLRTHKKDHH SRRGLLKMVG RRRHLLNYLR SKDIQRYREL IKSLGIRR

+
Macromolecule #16: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 10.12269 KDa
SequenceString:
AVKIRLTRLG SKRNPFYRIV VADARSPRDG RIIEQIGTYN PTSANAPEIK VDEALALKWL NDGAKPTDTV HNILSKEGIM KKFDEQKKA K

+
Macromolecule #17: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 10.065691 KDa
SequenceString:
SERNDRKVYV GKVVSDKMDK TITVLVETYK THKLYGKRVK YSKKYKTHDE NNSAKLGDIV KIQETRPLSA TKRFRLVEIV EESVII

+
Macromolecule #18: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 8.418959 KDa
SequenceString:
GRRRKKVCYF TANGITHIDY KDTELLKRFI SERGKILPRR VTGTSAKYQR MLTTAIKRSR HMALLPYVKE E

+
Macromolecule #19: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 9.605988 KDa
SequenceString:
SIKKGPFVDE HLMKKVEAQE GSEKKQVIKT WSRRSTIFPN FIGHTFAVYD GRKHVPVYVT EDMVGHKLGE FAPTRTFKGH VADD

+
Macromolecule #20: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 8.708018 KDa
SequenceString:
NIKSAIKRVK TTEKAEARNI SQKSAMRTAV KNAKTAVSNN ADNKNELVSL AVKLVDKAAQ SNLIHSNKAD RIKSQLMTAN

+
Macromolecule #23: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 29.956789 KDa
SequenceString: AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG ...String:
AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG KYVLIRLRSG EVRMILSTCR ATIGQVGNLQ HELVNVGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRPSPMS PWGKPTLGKK TRRGKKSSDK LIVRGRKK

+
Macromolecule #24: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 23.499877 KDa
SequenceString: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM ...String:
TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM PGRMGGNTVT VQNLEVVQVD TENKVILVKG NVPGPKKGLV EIRTSIKKGN

+
Macromolecule #25: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 21.666725 KDa
SequenceString: NYDVLKLDGT KSGSIELSDA VFGIEPNNSV LFEAINLQRA SLRQGTHAVK NRSAVSGGGR KPWKQKGTGR ARQGTIRAPQ WRGGGIVFG PTPRSYAYKM PKKMRRLALR SALSFKAQEN GLTVVDAFNF EAPKTKEFKN VLSTLEQPKK VLVVTENEDV N VELSARNI ...String:
NYDVLKLDGT KSGSIELSDA VFGIEPNNSV LFEAINLQRA SLRQGTHAVK NRSAVSGGGR KPWKQKGTGR ARQGTIRAPQ WRGGGIVFG PTPRSYAYKM PKKMRRLALR SALSFKAQEN GLTVVDAFNF EAPKTKEFKN VLSTLEQPKK VLVVTENEDV N VELSARNI PGVQVTTAQG LNVLDITNAD SLVITEAAAK K

+
Macromolecule #26: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 18.729721 KDa
SequenceString:
KEKFNTEVTE NLMKKFNYSS VMEVPKIDKI VVNMGVGDAV QNSKVLDNAV EELELITGQK PLVTKAKKSI ATFRLREGMP IGAKVTLRG ERMYEFLDKL ISVSLPRVRD FQGVSKKAFD GRGNYTLGVK EQLIFPEIDY DKVSKVRGMD IVIVTTANTD E EARELL

+
Macromolecule #27: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 18.296711 KDa
SequenceString:
IIDIPSDVTV TFDGNHVTVK GPKGELSRTL NERMTFKQEE NTIEVVRPSD SKEDRTNHGT TRALLNNMVQ GVSQGYVKVL ELVGVGYRA QMQGKDLILN VGYSHPVEIK AEENITFSVE KNTVVKVEGI SKEQVGALAS NIRSVRPPEP YKGKGIRYQG E YVRR

+
Macromolecule #28: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 16.359427 KDa
SequenceString:
MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

+
Macromolecule #29: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 13.157342 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

+
Macromolecule #30: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 14.193304 KDa
SequenceString:
MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS AS

+
Macromolecule #31: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 15.943755 KDa
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREELGG ET

+
Macromolecule #32: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 13.42035 KDa
SequenceString:
RKLGRTSDQR KAMLRDLATS LIISERIETT EARAKEVRSV VEKLITLGKK GDLASRRNAA KTLRNVEILN EDETTQTALQ KLFGEIAER YTERQGGYTR ILKQGPRRGD GAESVIIELV

+
Macromolecule #33: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 13.124093 KDa
SequenceString:
MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF

+
Macromolecule #34: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 12.594825 KDa
SequenceString:
MTNHKLIEAV TKSQLRTDLP SFRPGDTLRV HVRIIEGTRE RIQVFEGVVI KRRGGGVSET FTVRKISSGV GVERTFPLHT PKIEKIEVK RRGKVRRAKL YYLRSLRGKA A

+
Macromolecule #35: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 13.459919 KDa
SequenceString:
PRVKGGTVTR ARRKKTIKLA KGYFGSKHTL YKVAKQQVMK SGQYAFRDRR QRKRDFRKLW ITRINAAARQ HEMSYSRLMN GLKKAGIDI NRKMLSEIAI SDEKAFAQLV TKAKDAL

+
Macromolecule #36: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 11.354081 KDa
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

+
Macromolecule #37: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 12.328358 KDa
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKE

+
Macromolecule #38: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 10.3801 KDa
SequenceString:
EARDILKRPV ITEKSSEAMA EDKYTFDVDT RVNKTQVKMA VEEIFNVKVA SVNIMNYKPK KKRMGRYQGY TNKRRKAIVT LKEGSIDLF

+
Macromolecule #39: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 10.976878 KDa
SequenceString:
IKKGDNVKVI AGKDKGKEGK VIATLPKKDR VVVEGVNIMK KHQKPTQLNP EGGILETEAA IHVSNVQLLD PKTNEPTRVG YKFVDGKKV RIAKKSGEEI K

+
Macromolecule #40: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 10.522318 KDa
SequenceString:
SLKSIIRQGK QTRSDLKQLR KSGKVPAVVY GYGTKNVSVK VDEVEFIKVI REVGRNGVIE LGVGSKTIKV MVADYQFDPL KNQITHIDF LAINM

+
Macromolecule #41: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 8.911099 KDa
SequenceString:
KKGVSSTKNG RDSESKRLGA KRADGQFVTG GSILYRQRGT KIYPGENVGR GGDDTLFAKI DGVVKFERKG RDKKQVSVYA VA

+
Macromolecule #42: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 5.312262 KDa
SequenceString:
RKASTGNRRS HALNSTKRRW NANLQKVRIL VDGKPKKVWV SARALK

+
Macromolecule #43: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 7.660762 KDa
SequenceString:
KAKEIRDLTT SEIEEQIKSS KEELFNLRFQ LATGQLEETA RIRTVRKTIA RLKTVARERE IEQSK

+
Macromolecule #44: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 6.305308 KDa
SequenceString:
AKLQITLTRS VIGRPETQRK TVEALGLKKT NSSVVVEDNP AIRGQINKVK HLVTVEE

+
Macromolecule #45: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 9.737912 KDa
SequenceString:
MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYTG RQKFAAADGR VERFNKKFGL KSNN

+
Macromolecule #46: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 6.397427 KDa
SequenceString:
AVPKRRTSKT RKNKRRTHFK ISVPGMTECP NCGREYKLSH RVCKNCGSYN GEEVAA

+
Macromolecule #47: 50S ribosomal protein L33 2

MacromoleculeName: 50S ribosomal protein L33 2 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 4.711371 KDa
SequenceString:
ACTECGDRNY ITTKNKRNNP ERVEMKKFCS RENKQTLHR

+
Macromolecule #48: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 5.323446 KDa
SequenceString:
VKRTYQPNKR KHSKVHGFRK RMSTKNGRKV LARRRRKGRK VLSA

+
Macromolecule #49: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 6.985402 KDa
SequenceString:
PKMKTHRGAA KRVKRTASGQ LKRSRAFTSH LFANKSTKQK RQLRKARLVS KSDMKRVKQL

+
Macromolecule #50: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

+
Macromolecule #51: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 51 / Number of copies: 31 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #52: OXYGEN ATOM

MacromoleculeName: OXYGEN ATOM / type: ligand / ID: 52 / Number of copies: 9 / Formula: O
Molecular weightTheoretical: 15.999 Da
Chemical component information

ChemComp-O:
OXYGEN ATOM / Oxygen

+
Macromolecule #53: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 53 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #54: water

MacromoleculeName: water / type: ligand / ID: 54 / Number of copies: 162 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
Details: 5 mM Hepes-KOH pH 7.5 50 mM KCl 10 mM NH4Cl 10 mM Mg(OAc)2 1 mM DTT
GridMaterial: COPPER / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: blot force 5, blot waiting time 30 s.

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DARK FIELD
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 2-8 / Number grids imaged: 1 / Number real images: 3850 / Average exposure time: 1.0 sec. / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 423497
CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4wce


Details: also: homology model from 3J9W
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 1.3)
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 1.3)
Final reconstructionNumber classes used: 8 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.3) / Number images used: 110000

-
Atomic model buiding 1

RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-5li0:
70S ribosome from Staphylococcus aureus

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more