eF-site ID 7dfr-A PDB Code 7dfr Chain A click to enlarge Title CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. THE NADP+ HOLOENZYME AND THE FOLATE(DOT)NADP+ TERNARY COMPLEX. SUBSTRATE BINDING AND A MODEL FOR THE TRANSITION STATE Classification OXIDOREDUCTASE Compound DIHYDROFOLATE REDUCTASE Source Escherichia coli (strain K12) (DYR_ECOLI) Sequence A:  MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLDKPV IMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDE AIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVE GDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR Description (1)  DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) (DHFR) COMPLEX WITH FOLATE AND NADP+ Functional site 1) chain A residue 5 type sequence I description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 2) chain A residue 7 type sequence A description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 3) chain A residue 20 type sequence M description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 4) chain A residue 27 type sequence D description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 5) chain A residue 28 type sequence L description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 6) chain A residue 31 type sequence F description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 7) chain A residue 32 type sequence K description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 8) chain A residue 46 type sequence T description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 9) chain A residue 50 type sequence I description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 10) chain A residue 57 type sequence R description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 11) chain A residue 94 type sequence I description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 12) chain A residue 113 type sequence T description BINDING SITE FOR RESIDUE FOL A 161 source : AC1 13) chain A residue 6 type sequence A description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 14) chain A residue 7 type sequence A description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 15) chain A residue 14 type sequence I description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 16) chain A residue 16 type sequence M description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 17) chain A residue 18 type sequence N description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 18) chain A residue 19 type sequence A description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 19) chain A residue 20 type sequence M description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 20) chain A residue 22 type sequence W description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 21) chain A residue 43 type sequence G description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 22) chain A residue 44 type sequence R description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 23) chain A residue 45 type sequence H description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 24) chain A residue 46 type sequence T description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 25) chain A residue 49 type sequence S description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 26) chain A residue 62 type sequence L description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 27) chain A residue 63 type sequence S description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 28) chain A residue 64 type sequence S description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 29) chain A residue 76 type sequence K description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 30) chain A residue 94 type sequence I description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 31) chain A residue 96 type sequence G description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 32) chain A residue 97 type sequence G description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 33) chain A residue 98 type sequence R description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 34) chain A residue 99 type sequence V description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 35) chain A residue 100 type sequence Y description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 36) chain A residue 102 type sequence Q description BINDING SITE FOR RESIDUE NAP A 164 source : AC2 37) chain A residue 13-35 type prosite sequence VIGMENAMPWNLPADLAWFKRNT description DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT source prosite : PS00075 38) chain A residue 113 type BINDING sequence T description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 39) chain A residue 5 type BINDING sequence I description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 40) chain A residue 27 type BINDING sequence D description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 41) chain A residue 52 type BINDING sequence R description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 42) chain A residue 57 type BINDING sequence R description BINDING => ECO:0000305|PubMed:9012674 source Swiss-Prot : SWS_FT_FI1 43) chain A residue 7 type BINDING sequence A description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 44) chain A residue 13 type BINDING sequence V description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 45) chain A residue 45 type BINDING sequence H description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 46) chain A residue 63 type BINDING sequence S description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 47) chain A residue 76 type BINDING sequence K description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2 48) chain A residue 95 type BINDING sequence G description BINDING => ECO:0000269|PubMed:19374017 source Swiss-Prot : SWS_FT_FI2

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