eF-site ID 6wdm-1234567 PDB Code 6wdm Chain 1, 2, 3, 4, 5, 6, 7 Title Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B2) Classification RIBOSOME Compound 50S ribosomal protein L2 Source ORGANISM_SCIENTIFIC: Escherichia coli; Sequence 1:  GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGU CAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCG GUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAU GGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGA AUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAU CUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCC AGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAU CAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGA UACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGU GAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUG AAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGAC UGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAA GAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUG UACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGC GUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAG CAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUC UUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCC GGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACU AACUGGAGGACCGAACCGACUAAUGUCGAAAAAUUAGCGG AUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGA GAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUC GUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGG GGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUA CCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAA CGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUA AGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAA GGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCA UUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUG CGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUG CGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUC UGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGU AUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGG GUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCC AACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAG GCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCU GUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAU GUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGC UGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGU GAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUG CUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUC GUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCA AGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAU GGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGU GAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUAC CAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGC AAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGG UGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCU CUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAAC GGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGA CCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACC CGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUG UACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUA UAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAG GUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGC CGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAAC GUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGU AGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGG AGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGU UAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACG GCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGG UUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUAC UCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUC GACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUC CUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCA UUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGA CAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAG GGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAU CACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGU AGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGC ACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAA GGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGC CGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUAC UAAUGAACCGUGAGGCUUAACCU 2:  UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAU GCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGU GUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAA 3:  AAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCG GCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAA GCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAU GUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAA ACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGG GGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGG GAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACG AUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAA CUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGG GAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCC GCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCA GCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUG ACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAG CAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAU UACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAG AUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAU ACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCA GGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCG GUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGG UGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGU AGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCC UUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCG CCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUU GACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUU CGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCAC GGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGUG AGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAA AUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCU UUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGC CAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCA UCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUG GCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGAC CUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACU CGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAG AAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCG CCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUA GCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAU GACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACC UGCGGUUGGAUCACCUCCU 4:  AGGAGGUAAAAAUGCUCA 5:  CGCGGGGUGGAGCAGCCUGGUAGCUCGUCGGGCUCAUAAC CCGAAGGUCGUCGGUUCAAAUCCGGCCCCCGCAACCA 6:  CGCGGGGUGGAGCAGCCUGGUAGCUCGUCGGGCUCAUAAC CCGAAGGUCGUCGGUUCAAAUCCGGCCCCCGCAACCA 7:  GCCCGGAUAGCUCAGUCGGUAGAGCAGGGGAUUGAAAAUC CCCGUGUCCUUGGUUCGAUUCCGAGUCCGGGCACCA Description Functional site 1) chain 1 residue 2451 type sequence A description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 2) chain 1 residue 2452 type sequence C description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 3) chain 1 residue 2506 type sequence U description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 4) chain 1 residue 2553 type sequence G description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 5) chain 1 residue 2583 type sequence G description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 6) chain 1 residue 2584 type sequence U description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 7) chain 1 residue 2585 type sequence U description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 8) chain 1 residue 2602 type sequence A description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 9) chain 5 residue 76 type sequence A description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 10) chain 7 residue 75 type sequence C description binding site for Di-nucleotide PHE 7 101 and A 7 76 source : AC1 11) chain 1 residue 2061 type sequence G description binding site for Di-peptide PHE 7 101 and FME 7 102 source : AC2 12) chain 1 residue 2451 type sequence A description binding site for Di-peptide PHE 7 101 and FME 7 102 source : AC2 13) chain 1 residue 2452 type sequence C description binding site for Di-peptide PHE 7 101 and FME 7 102 source : AC2 14) chain 1 residue 2506 type sequence U description binding site for Di-peptide PHE 7 101 and FME 7 102 source : AC2 15) chain 1 residue 2585 type sequence U description binding site for Di-peptide PHE 7 101 and FME 7 102 source : AC2 16) chain 5 residue 76 type sequence A description binding site for Di-peptide PHE 7 101 and FME 7 102 source : AC2 17) chain 7 residue 76 type sequence A description binding site for Di-peptide PHE 7 101 and FME 7 102 source : AC2

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