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Title
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Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure IV-B1) |
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Classification
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RIBOSOME |
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Compound
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50S ribosomal protein L2 |
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Source
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ORGANISM_SCIENTIFIC: Escherichia coli; |
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Sequence
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1: |
GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGU
CAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCG
GUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAU
GGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGA
AUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAU
CUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCC
AGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAU
CAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGA
UACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGU
GAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUG
AAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGAC
UGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAA
GAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUG
UACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGC
GUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAG
CAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUC
UUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCC
GGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACU
AACUGGAGGACCGAACCGACUAAUGUCGAAAAAUUAGCGG
AUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGA
GAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUC
GUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGG
GGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUA
CCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAA
CGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUA
AGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAA
GGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCA
UUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUG
CGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUG
CGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUC
UGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGU
AUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGG
GUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCC
AACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAG
GCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCU
GUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAU
GUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGC
UGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGU
GAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUG
CUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUC
GUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCA
AGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAU
GGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGU
GAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUAC
CAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGC
AAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGG
UGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCU
CUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAAC
GGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGA
CCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACC
CGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUG
UACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUA
UAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAG
GUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGC
CGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAAC
GUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGU
AGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGG
AGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGU
UAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACG
GCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGG
UUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUAC
UCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUC
GACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUC
CUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCA
UUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGA
CAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAG
GGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAU
CACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGU
AGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGC
ACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAA
GGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGC
CGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUAC
UAAUGAACCGUGAGGCUUAACCU
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| 2: |
UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAU
GCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGU
GUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAA
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| 3: |
AAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCG
GCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAA
GCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAU
GUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAA
ACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGG
GGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGG
GAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACG
AUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAA
CUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGG
GAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCC
GCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCA
GCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUG
ACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAG
CAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAU
UACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAG
AUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAU
ACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCA
GGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCG
GUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGG
UGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGU
AGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCC
UUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCG
CCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUU
GACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUU
CGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCAC
GGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGUG
AGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAA
AUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCU
UUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGC
CAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCA
UCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUG
GCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGAC
CUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACU
CGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAG
AAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCG
CCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUA
GCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAU
GACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACC
UGCGGUUGGAUCACCUCCU
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| 4: |
AGGAGGUAAAAAUGCUCA
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| 5: |
CGCGGGGUGGAGCAGCCUGGUAGCUCGUCGGGCUCAUAAC
CCGAAGGUCGUCGGUUCAAAUCCGGCCCCCGCAACCA
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| 6: |
CGCGGGGUGGAGCAGCCUGGUAGCUCGUCGGGCUCAUAAC
CCGAAGGUCGUCGGUUCAAAUCCGGCCCCCGCAACCA
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| 7: |
GCCCGGAUAGCUCAGUCGGUAGAGCAGGGGAUUGAAAAUC
CCCGUGUCCUUGGUUCGAUUCCGAGUCCGGGCA
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Description
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