eF-site ID 6ne0-ABCDEFGHL PDB Code 6ne0 Chain A, B, C, D, E, F, G, H, L click to enlarge Title Structure of double-stranded target DNA engaged Csy complex from Pseudomonas aeruginosa (PA-14) Classification IMMUNE SYSTEM/RNA Compound CRISPR-associated protein Csy1 Source (6NE0) Sequence A:  QELRQFIESFIQERLQGKLDKLQPDEDDKRQTLLATHRRE AWLADAARRVGQLQLVTHTLKPIHPDARGSNLHSLPQAPG QPGLAGSHELGDRLVSDVVGNAAALDVFKFLSLQYQGKNL LNWLTEDSAEALQALSDNAEQAREWRQAFIGITTVKGAPA SHSLAKQLYFPLPGSGYHLLAPLFPTSLVHHVHALLREAR FGDAAKAAREARSRQESWPHGFSEYPNLAIQKFGGTKPQN ISQLNNERRGENWLLPSLPPNWQRQNVNAPEVSRLTRTLQ RFLAKTVHNNLAIRQRRAQLVAQICDEALQYAARLRELEP GWSATPGCQLHDAEQLWLDPLRAQTDETFLQRRLRGDWPA EVGNRFANWLNRAVSSDSQILGSPEAAQWSQELSKELTMF K B:  VTDPEALLLLPRLSIQNANAISSPLTWGFPSPGAFTGFVH ALQRRVGISLDIELDGVGIVCHRFEAQISQPAGKRTKVFN LTRNPLNRDGSTAAIVEEGRAHLEVSLLLGVHGDGLDDHP AQEIARQVQEQAGAMRLAGGSILPWCNERFPAPNAELLML GGSDEQRRKNQRRLTRRLLPGFALVSREALLQQHLETLRT TLPEATTLDALLDLCRINFEPWQVRDKPGWLVPIPAGYNA LSPLYLPGEVRNARDRETPLRFVENLFGLGEWLSPHRVAA LSDLLWYHHAEPDKGLYRWSTPRFV C:  LSTASVLAFERKLDPSDALMSAGAWAQRDASQEWPAVTVR EKSQTVDVANLPSDADTLKVRFTLRVLGGAGTPSACNDAA YRDKLLQTVATYVNDQGFAELARRYAHNLANARFLWRNRV GAEAVEVRINHIRQGEVARAWRFDALAIGLRDFKADAELD ALAELIASGLSGSGHVLLEVVAFARIGDGQEVFPSQELKT LYSVRDAAAIHSQKIGNALRTIDTWYPDEDGLGPIAVEPY GSVTSQGKAYRQPKQKLDFYTLLDNWVLRDEAPAVEQQHY VIANLIRGGVFGE D:  LSTASVLAFERKLDPSDALMSAGAWAQRDASQEWPAVTVR EKSVRGTISNRLKTKDRDPAKLDASIQSPNLQTVDVANLP SDADTLKVRFTLRVLGGAGTPSACNDAAYRDKLLQTVATY VNDQGFAELARRYAHNLANARFLWRNRVGAEAVEVRINHI RQGEVARAWRFDALAIGLRDFKADAELDALAELIASGLSG SGHVLLEVVAFARIGDGQEVFPSQELILDKGDKKGQKSKT LYSVRDAAAIHSQKIGNALRTIDTWYPDEDGLGPIAVEPY GSVTSQGKAYRQPKQKLDFYTLLDNWVLRDEAPAVEQQHY VIANLIRGGVFGE E:  ILSTASVLAFERKLDPSDALMSAGAWAQRDASQEWPAVTV REKSVRGTISNRLKTKDRDPAKLDASIQSPNLQTVDVANL PSDADTLKVRFTLRVLGGAGTPSACNDAAYRDKLLQTVAT YVNDQGFAELARRYAHNLANARFLWRNRVGAEAVEVRINH IRQGEVARAWRFDALAIGLRDFKADAELDALAELIASGLS GSGHVLLEVVAFARIGDGQEVFPSQELILDKGDKKGQKSK TLYSVRDAAAIHSQKIGNALRTIDTWYPDEDGLGPIAVEP YGSVTSQGKAYRQPKQKLDFYTLLDNWVLRDEAPAVEQQH YVIANLIRGGVFGE F:  ILSTASVLAFERKLDPSDALMSAGAWAQRDASQEWPAVTV REKSVRGTISNRLKTKDRDPAKLDASIQSPNLQTVDVANL PSDADTLKVRFTLRVLGGAGTPSACNDAAYRDKLLQTVAT YVNDQGFAELARRYAHNLANARFLWRNRVGAEAVEVRINH IRQGEVARAWRFDALAIGLRDFKADAELDALAELIASGLS GSGHVLLEVVAFARIGDGQEVFPSQELILDKGDKKGQKSK TLYSVRDAAAIHSQKIGNALRTIDTWYPDEDGLGPIAVEP YGSVTSQGKAYRQPKQKLDFYTLLDNWVLRDEAPAVEQQH YVIANLIRGGVFGEA G:  LSTASVLAFERKLDPSDALMSAGAWAQRDASQEWPAVTVR EKSVRGTISNRLKTKDRDPAKLDASIQSPNLQTVDVANLP SDADTLKVRFTLRVLGGAGTPSACNDAAYRDKLLQTVATY VNDQGFAELARRYAHNLANARFLWRNRVGAEAVEVRINHI RQGEVARAWRFDALAIGLRDFKADAELDALAELIASGLSG SGHVLLEVVAFARIGDGQEVFPSQELILDKGDKKGQKSKT LYSVRDAAAIHSQKIGNALRTIDTWYPDEDGLGPIAVEPY GSVTSQGKAYRQPKQKLDFYTLLDNWVLRDEAPAVEQQHY VIANLIRGGVFGE H:  LSTASVLAFERKLDPSDALMSAGAWAQRDASQEWPAVTVR EKSVRGTISNRLKTKDRDPAKLDASIQSPNLQTVDVANLP SDADTLKVRFTLRVLGGAGTPSACNDAAYRDKLLQTVATY VNDQGFAELARRYAHNLANARFLWRNRVGAEAVEVRINHI RQGEVARAWRFDALAIGLRDFKADAELDALAELIASGLSG SGHVLLEVVAFARIGDGQEVFPSQELILDKGDKKGQKSKT LYSVRDAAAIHSQKIGNALRTIDTWYPDEDGLGPIAVEPY GSVTSQGKAYRQPKQKLDFYTLLDNWVLRDEAPAVEQQHY VIANLIRGGVFGE L:  FTMDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGD RIGVSFPDLDESRSRLGERLRIHASADDLRALLARPWLEG LRDHLQFGEPAVVPHPTPYRQVSRVQAKSNPERLRRRLMR RHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLF IRHGPLQVTAEEGGFTCYGLSKGGFVPWF Description Functional site 1) chain L residue 148 type SITE sequence S description Substrate binding source Swiss-Prot : SWS_FT_FI2 2) chain L residue 29 type ACT_SITE sequence H description Proton acceptor => ECO:0000305 source Swiss-Prot : SWS_FT_FI1

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