eF-site ID 6jnx-ABCDEFNPQRT PDB Code 6jnx Chain A, B, C, D, E, F, N, P, Q, R, T Title Cryo-EM structure of a Q-engaged arrested complex Classification TRANSCRIPTION,DNA,RNA Compound DNA-directed RNA polymerase subunit alpha Source (M1FPN0_9CAUD) Sequence A:  FLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRIL LSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKG LAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIVKPQ HVICHLTDENASISMRIKVQRGRGYVPASTRIRPIGRLLV DACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDP EEAIRRAATILAEQLEAFV B:  VTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLN LKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIV KPQHVICHLTDENASISMRIKVQRGRGYVPASTRRLLVDA CYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEE AIRRAATILAEQLEAFVD C:  VYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQ DPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFD VQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQE VYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDK GKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRR RKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMEL VPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDD VKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDL LAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLS ALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVG RMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGE VDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSL GDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPL SEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPI ETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTD EIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGE SSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALM GANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRG GVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQ NTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMR VAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSR DTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGD ILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVP NGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLS EELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELG LTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDD LAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINP IEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAA KGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDL STFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKL GDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDD KMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAY GAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPE SFNVLLKEIRSLGINIELED D:  EFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDG LFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQT KVRRERMGHIELASPTAHIWFLKSLPSRIGLLLDMPLRDI ERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDE FDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRK KLTKRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPL DGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNE KRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQ GRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALE LFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEV IREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCA AYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPAN GEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAER LYRSGLASLHARVKVRITEYEKDANGELVAKTSLKDTTVG RAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLK PTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIIS EAEAEVAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSK AMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAA QIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFIS THGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCG THEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTAD ILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVC AHCYGRDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRSS IQVKNKGSIKLSNVKSVVNSSGKLVITSRNTELKLIDEFG RTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVIT EVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTA GGKDLRPALKIVDAQGNDVLIPGTDMPAQYFLPGKAIVQL EDGVQISSGDTLARIPQGGLPRVADLFEARRPKEPAILAE ISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNV FEGERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQ DVYRLQGVKINDKHIEVIVRQMLRKATIVNAGSSDFLEGE QVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATES FISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIP AGTGYAYHQDRMRRR E:  ARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPE ENDKTTVIALREIEEGLINNQILDVRERQEQQEQEAAEL F:  EIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQ VQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDDP ELAREKFAELRAQYVVTRDTIKATAQEEILKLSEVFKQFR LVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKK NFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRA LQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMV EANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFE YRRGYKFSTYATWWIRQAITRSIADQA N:  CATCATTGAGCAAATGAGCAACACTATTCGCATAAGGTGG GAGTAGTGAGTCTTAAGTTGCAA P:  NLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFMES VEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLS YYAHGSSKHAIASYYHRVARPRKMLCRGGGRIQKPSLATC RREVDEILNASLFMIYPVLDSAFKNRK Q:  NLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFMES VDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIA SYYHRVARPRKMLCRGGGRIQKPSLATCRREVDEILNASL FMIYPV R:  AUAAGGUGGGGUUAGUGA T:  TTGCAACTTAAGACTCACTAACCCCACCTTATGCGAATAG TGTTGCTCATTTGCTCAATGATG Description Functional site 1) chain D residue 460 type sequence D description binding site for residue MG D 1501 source : AC1 2) chain D residue 462 type sequence D description binding site for residue MG D 1501 source : AC1 3) chain D residue 464 type sequence D description binding site for residue MG D 1501 source : AC1 4) chain R residue 18 type sequence A description binding site for residue MG D 1501 source : AC1 5) chain R residue 19 type sequence G description binding site for residue MG D 1501 source : AC1 6) chain D residue 70 type sequence C description binding site for residue ZN D 1502 source : AC2 7) chain D residue 72 type sequence C description binding site for residue ZN D 1502 source : AC2 8) chain D residue 85 type sequence C description binding site for residue ZN D 1502 source : AC2 9) chain D residue 87 type sequence K description binding site for residue ZN D 1502 source : AC2 10) chain D residue 88 type sequence C description binding site for residue ZN D 1502 source : AC2 11) chain D residue 814 type sequence C description binding site for residue ZN D 1503 source : AC3 12) chain D residue 883 type sequence R description binding site for residue ZN D 1503 source : AC3 13) chain D residue 888 type sequence C description binding site for residue ZN D 1503 source : AC3 14) chain D residue 889 type sequence D description binding site for residue ZN D 1503 source : AC3 15) chain D residue 895 type sequence C description binding site for residue ZN D 1503 source : AC3 16) chain D residue 898 type sequence C description binding site for residue ZN D 1503 source : AC3 17) chain C residue 1063-1075 type prosite sequence GDKMAGRHGNKGV description RNA_POL_BETA RNA polymerases beta chain signature. GdKMAGrHGNKGV source prosite : PS01166 18) chain F residue 403-416 type prosite sequence DLIQEGNIGLMKAV description SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnIGLMkAV source prosite : PS00715 19) chain D residue 895 type DNA_BIND sequence C description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 20) chain D residue 898 type DNA_BIND sequence C description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 21) chain D residue 460 type DNA_BIND sequence D description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 22) chain D residue 462 type DNA_BIND sequence D description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 23) chain D residue 464 type DNA_BIND sequence D description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 24) chain D residue 814 type DNA_BIND sequence C description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 25) chain D residue 888 type DNA_BIND sequence C description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 26) chain D residue 72 type DNA_BIND sequence C description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 27) chain D residue 85 type DNA_BIND sequence C description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1 28) chain D residue 88 type DNA_BIND sequence C description H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00963 source Swiss-Prot : SWS_FT_FI1

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