eF-site ID 6gte-A PDB Code 6gte Chain A click to enlarge Title Transient state structure of CRISPR-Cpf1 (Cas12a) I3 conformation Classification HYDROLASE Compound CRISPR-associated endonuclease Cas12a Source (6GTE) Sequence A:  IYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEK RAKDYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDV YFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFKNL FNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDI DEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYR IVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEEL TFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFN TIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMS VLFKQILSDTEKLEDDSDVVTTMQSFYEQIAAFKTVEEKS IKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDD YSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAK YLSLETIKLALEEFNKHRDIDKQCRFEEILANFAAIPMIF DEIAQNKDNLAQISIKYQNQGKKDLLQASAEDDVKAIKDL LDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECY FELANIVPLYNKIRNYITQKPYSDEKFKLNFENSTLANGW DKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKEN KGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDIL RIRNHSTHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSIS KHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENI SESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWK ALFDERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAI ANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFK SSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLV DGKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARK DWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFQDLN QVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPF ETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYES VSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGK WTIASFGSRLINFRNSNWDTREVYPTKELEKLLKDYSIEY GHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLK GLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRN Description (1)  CRISPR-associated endonuclease Cas12a/RNA Complex Functional site 1) chain A residue 852 type SITE sequence K description Stabilizes transition state for pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI14 2) chain A residue 1026 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI15 3) chain A residue 1063 type SITE sequence T description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI15 4) chain A residue 843 type ACT_SITE sequence H description For pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI1 5) chain A residue 852 type ACT_SITE sequence K description For pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI1 6) chain A residue 869 type ACT_SITE sequence K description For pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI1 7) chain A residue 917 type ACT_SITE sequence D description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 8) chain A residue 1006 type ACT_SITE sequence Q description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 9) chain A residue 1255 type ACT_SITE sequence D description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 10) chain A residue 16 type SITE sequence T description Binds crRNA alone and in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI3 11) chain A residue 131 type SITE sequence K description Binds target strand DNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI4 12) chain A residue 295 type SITE sequence T description Binds crRNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI5 13) chain A residue 320 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 14) chain A residue 589 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 15) chain A residue 410 type SITE sequence Y description Caps the crRNA-target DNA heteroduplex => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI7 16) chain A residue 613 type SITE sequence K description Binds DNA protospacer adjacent motif (PAM) => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI8 17) chain A residue 667 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI9 18) chain A residue 671 type SITE sequence K description Binds PAM => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI10 19) chain A residue 823 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI11 20) chain A residue 677 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI11 21) chain A residue 826 type SITE sequence G description Binds Target strand DNA; via amide nitrogen => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI12 22) chain A residue 833 type SITE sequence R description Binds crRNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI13

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