eF-site ID 6gam-M
PDB Code 6gam
Chain M

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Title Structure of E14Q variant of E. coli hydrogenase-2 (as-isolated enzyme)
Classification OXIDOREDUCTASE
Compound Hydrogenase-2 small chain
Source (MBHM_ECOLI)
Sequence M:  SQRITIDPVTRIQGHLRIDCEIENGVVSKAWASGTMWRGM
EEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALN
IDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITS
ALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVAS
GQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDA
NRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLM
YIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVN
YLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDE
YLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGK
YSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEI
VAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQN
QYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRGM
LSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQS
LVGTPVADPNKPLEVVRTIHSFDPCMACAVH
Description


Functional site

1) chain M
residue 211
type
sequence K
description binding site for residue F3S T 402
source : AC8

2) chain M
residue 216
type
sequence Q
description binding site for residue F3S T 402
source : AC8

3) chain M
residue 59
type
sequence R
description binding site for residue SF4 T 403
source : AC9

4) chain M
residue 64
type
sequence C
description binding site for residue FCO M 601
source : AD1

5) chain M
residue 67
type
sequence T
description binding site for residue FCO M 601
source : AD1

6) chain M
residue 68
type
sequence H
description binding site for residue FCO M 601
source : AD1

7) chain M
residue 477
type
sequence A
description binding site for residue FCO M 601
source : AD1

8) chain M
residue 478
type
sequence P
description binding site for residue FCO M 601
source : AD1

9) chain M
residue 479
type
sequence R
description binding site for residue FCO M 601
source : AD1

10) chain M
residue 482
type
sequence L
description binding site for residue FCO M 601
source : AD1

11) chain M
residue 500
type
sequence V
description binding site for residue FCO M 601
source : AD1

12) chain M
residue 501
type
sequence P
description binding site for residue FCO M 601
source : AD1

13) chain M
residue 502
type
sequence S
description binding site for residue FCO M 601
source : AD1

14) chain M
residue 549
type
sequence C
description binding site for residue FCO M 601
source : AD1

15) chain M
residue 61
type
sequence C
description binding site for residue NI M 602
source : AD2

16) chain M
residue 64
type
sequence C
description binding site for residue NI M 602
source : AD2

17) chain M
residue 546
type
sequence C
description binding site for residue NI M 602
source : AD2

18) chain M
residue 549
type
sequence C
description binding site for residue NI M 602
source : AD2

19) chain M
residue 42
type
sequence E
description binding site for residue MG M 603
source : AD3

20) chain M
residue 498
type
sequence A
description binding site for residue MG M 603
source : AD3

21) chain M
residue 552
type
sequence H
description binding site for residue MG M 603
source : AD3

22) chain M
residue 552
type SITE
sequence H
description Cleavage; by HybD
source Swiss-Prot : SWS_FT_FI2

23) chain M
residue 61
type BINDING
sequence C
description BINDING => ECO:0000255
source Swiss-Prot : SWS_FT_FI1

24) chain M
residue 64
type BINDING
sequence C
description BINDING => ECO:0000255
source Swiss-Prot : SWS_FT_FI1

25) chain M
residue 546
type BINDING
sequence C
description BINDING => ECO:0000255
source Swiss-Prot : SWS_FT_FI1

26) chain M
residue 549
type BINDING
sequence C
description BINDING => ECO:0000255
source Swiss-Prot : SWS_FT_FI1


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