eF-site ID 6gam-L PDB Code 6gam Chain L click to enlarge Title Structure of E14Q variant of E. coli hydrogenase-2 (as-isolated enzyme) Classification OXIDOREDUCTASE Compound Hydrogenase-2 small chain Source (MBHM_ECOLI) Sequence L:  SQRITIDPVTRIQGHLRIDCEIENGVVSKAWASGTMWRGM EEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALN IDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITS ALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVAS GQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDA NRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLM YIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVN YLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDE YLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGK YSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEI VAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQN QYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRGM LSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQS LVGTPVADPNKPLEVVRTIHSFDPCMACAVH Description Functional site 1) chain L residue 211 type sequence K description binding site for residue F3S S 402 source : AC2 2) chain L residue 216 type sequence Q description binding site for residue F3S S 402 source : AC2 3) chain L residue 59 type sequence R description binding site for residue SF4 S 403 source : AC3 4) chain L residue 64 type sequence C description binding site for residue FCO L 601 source : AC4 5) chain L residue 67 type sequence T description binding site for residue FCO L 601 source : AC4 6) chain L residue 68 type sequence H description binding site for residue FCO L 601 source : AC4 7) chain L residue 477 type sequence A description binding site for residue FCO L 601 source : AC4 8) chain L residue 478 type sequence P description binding site for residue FCO L 601 source : AC4 9) chain L residue 479 type sequence R description binding site for residue FCO L 601 source : AC4 10) chain L residue 482 type sequence L description binding site for residue FCO L 601 source : AC4 11) chain L residue 500 type sequence V description binding site for residue FCO L 601 source : AC4 12) chain L residue 501 type sequence P description binding site for residue FCO L 601 source : AC4 13) chain L residue 502 type sequence S description binding site for residue FCO L 601 source : AC4 14) chain L residue 549 type sequence C description binding site for residue FCO L 601 source : AC4 15) chain L residue 61 type sequence C description binding site for residue NI L 602 source : AC5 16) chain L residue 64 type sequence C description binding site for residue NI L 602 source : AC5 17) chain L residue 546 type sequence C description binding site for residue NI L 602 source : AC5 18) chain L residue 549 type sequence C description binding site for residue NI L 602 source : AC5 19) chain L residue 42 type sequence E description binding site for residue MG L 603 source : AC6 20) chain L residue 498 type sequence A description binding site for residue MG L 603 source : AC6 21) chain L residue 552 type sequence H description binding site for residue MG L 603 source : AC6 22) chain L residue 61 type BINDING sequence C description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 23) chain L residue 64 type BINDING sequence C description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 24) chain L residue 546 type BINDING sequence C description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 25) chain L residue 549 type BINDING sequence C description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 26) chain L residue 552 type SITE sequence H description Cleavage; by HybD source Swiss-Prot : SWS_FT_FI2 27) chain L residue 39-64 type prosite sequence RGMEEIVKNRDPRDAWMIVQRICGVC description NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGMEeivknrdprdawmivQRiCGVC source prosite : PS00507 28) chain L residue 543-552 type prosite sequence FDPCMACAVH description NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCMACav.H source prosite : PS00508

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