|
|
eF-site ID
|
6fb6-DEFG |
|
PDB Code
|
6fb6 |
|
Chain
|
D, E, F, G |
|
click to enlarge
|
|
|
Title
|
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Manganese |
|
Classification
|
DNA BINDING PROTEIN |
|
Compound
|
I-CreI monomer A |
|
Source
|
(6FB6) |
|
|
Sequence
|
D: |
TCAGACTTGTCCAC
|
| E: |
GGGAGTCAGA
|
| F: |
TCTGACTCCTGTGG
|
| G: |
ACAAGTCTGA
|
|
|
Description
|
|
Functional site
|
|
|
1)
|
chain |
D |
| residue |
514 |
| type |
|
| sequence |
C
|
| description |
binding site for residue MN A 201
|
| source |
: AC1
|
|
|
2)
|
chain |
G |
| residue |
615 |
| type |
|
| sequence |
A
|
| description |
binding site for residue MN A 201
|
| source |
: AC1
|
|
|
3)
|
chain |
D |
| residue |
514 |
| type |
|
| sequence |
C
|
| description |
binding site for residue MN A 202
|
| source |
: AC2
|
|
|
4)
|
chain |
E |
| residue |
515 |
| type |
|
| sequence |
G
|
| description |
binding site for residue MN A 202
|
| source |
: AC2
|
|
|
5)
|
chain |
F |
| residue |
614 |
| type |
|
| sequence |
G
|
| description |
binding site for residue MN A 202
|
| source |
: AC2
|
|
|
6)
|
chain |
G |
| residue |
615 |
| type |
|
| sequence |
A
|
| description |
binding site for residue MN A 202
|
| source |
: AC2
|
|
|
7)
|
chain |
E |
| residue |
515 |
| type |
|
| sequence |
G
|
| description |
binding site for residue MN A 203
|
| source |
: AC3
|
|
|
8)
|
chain |
F |
| residue |
614 |
| type |
|
| sequence |
G
|
| description |
binding site for residue MN A 203
|
| source |
: AC3
|
|
|
|