|
|
eF-site ID
|
6fb2-AB |
|
PDB Code
|
6fb2 |
|
Chain
|
A, B |
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click to enlarge
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Title
|
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Manganese |
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Classification
|
DNA BINDING PROTEIN |
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Compound
|
DNA endonuclease I-CreI |
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Source
|
(6FB2) |
|
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Sequence
|
A: |
NTKYNKEFLLYLAGFVDSDGSIIAQIKPNQSVKFKHRLQL
TFDVTQKTQRRWFLDKLVDEIGVGYVADSGSVSKYRLSEI
KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK
FLEVCTWVDQIAALNDSKTRKTTSETVRAVLDS
|
| B: |
NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSGKFKHKLSL
TFKVTQKTQRRWFLDKLVDEIGVGYVYDSGSVSNYYLSEI
KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK
FLEVCTWVDQVAALNDSKTRKTTSETVRAVLDSL
|
|
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Description
|
|
Functional site
|
|
|
1)
|
chain |
A |
| residue |
20 |
| type |
|
| sequence |
D
|
| description |
binding site for residue MN A 201
|
| source |
: AC1
|
|
|
2)
|
chain |
B |
| residue |
20 |
| type |
|
| sequence |
D
|
| description |
binding site for residue MN A 201
|
| source |
: AC1
|
|
|
3)
|
chain |
A |
| residue |
19 |
| type |
|
| sequence |
S
|
| description |
binding site for residue MN A 202
|
| source |
: AC2
|
|
|
4)
|
chain |
B |
| residue |
20 |
| type |
|
| sequence |
D
|
| description |
binding site for residue MN A 202
|
| source |
: AC2
|
|
|
5)
|
chain |
A |
| residue |
20 |
| type |
|
| sequence |
D
|
| description |
binding site for residue MN A 203
|
| source |
: AC3
|
|
|
6)
|
chain |
B |
| residue |
19 |
| type |
|
| sequence |
G
|
| description |
binding site for residue MN A 203
|
| source |
: AC3
|
|
|
7)
|
chain |
B |
| residue |
19 |
| type |
catalytic |
| sequence |
G
|
| description |
233
|
| source |
MCSA : MCSA1
|
|
|
8)
|
chain |
B |
| residue |
20 |
| type |
catalytic |
| sequence |
D
|
| description |
233
|
| source |
MCSA : MCSA1
|
|
|
9)
|
chain |
B |
| residue |
20 |
| type |
BINDING |
| sequence |
D
|
| description |
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
10)
|
chain |
B |
| residue |
19 |
| type |
BINDING |
| sequence |
G
|
| description |
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
|