|
|
eF-site ID
|
6fb1-DEFG |
|
PDB Code
|
6fb1 |
|
Chain
|
D, E, F, G |
|
click to enlarge
|
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Title
|
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Magnesium |
|
Classification
|
DNA BINDING PROTEIN |
|
Compound
|
DNA endonuclease I-CreI |
|
Source
|
(6FB1) |
|
|
Sequence
|
D: |
TCAGACTTCTCCAC
|
| E: |
AGGAGTCAGA
|
| F: |
TCTGACTCCTGTGG
|
| G: |
AGAAGTCTGA
|
|
|
Description
|
|
Functional site
|
|
|
1)
|
chain |
E |
| residue |
515 |
| type |
|
| sequence |
A
|
| description |
binding site for residue MG A 201
|
| source |
: AC1
|
|
|
2)
|
chain |
F |
| residue |
614 |
| type |
|
| sequence |
G
|
| description |
binding site for residue MG A 201
|
| source |
: AC1
|
|
|
3)
|
chain |
D |
| residue |
514 |
| type |
|
| sequence |
C
|
| description |
binding site for residue MG A 202
|
| source |
: AC2
|
|
|
4)
|
chain |
E |
| residue |
515 |
| type |
|
| sequence |
A
|
| description |
binding site for residue MG A 202
|
| source |
: AC2
|
|
|
5)
|
chain |
F |
| residue |
614 |
| type |
|
| sequence |
G
|
| description |
binding site for residue MG A 202
|
| source |
: AC2
|
|
|
6)
|
chain |
G |
| residue |
615 |
| type |
|
| sequence |
A
|
| description |
binding site for residue MG A 202
|
| source |
: AC2
|
|
|
7)
|
chain |
D |
| residue |
514 |
| type |
|
| sequence |
C
|
| description |
binding site for residue MG A 203
|
| source |
: AC3
|
|
|
8)
|
chain |
G |
| residue |
615 |
| type |
|
| sequence |
A
|
| description |
binding site for residue MG A 203
|
| source |
: AC3
|
|
|
|