eF-site ID 6dve-ABCDEFGH PDB Code 6dve Chain A, B, C, D, E, F, G, H Title Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF selenomethionine-labelled sigma factor L) with 6 nt spacer Classification TRANSFERASE/DNA Compound DNA-directed RNA polymerase subunit alpha Source (6DVE) Sequence A:  LISQRPTLSEDVLTDNRSQFVIEPLEPGFGYTLGNSLRRT LLSSIPGAAVTSIRIDGVLHEFTTVPGVKEDVTEIILNLK SLVVSSEEDEPVTMYLRKQGPGEVTAGDIVPPAGVTVHNP GMHIATLNDKGKLEVELVVERGRGYVPAVQNRASGAEIGR IPVDSIYSPVLKVTYKVDATRVEQRTDFDKLILDVETKNS ISPRDALASAGKTLVELFGLARELN B:  MLISQRPTLSEDVLTDNRSQFVIEPLEPGFGYTLGNSLRR TLLSSIPGAAVTSIRIDGVLHEFTTVPGVKEDVTEIILNL KSLVVSSEEDEPVTMYLRKQGPGEVTAGDIVPPAGVTVHN PGMHIATLNDKGKLEVELVVERGRGYVPAVQNRASGAEIG RIPVDSIYSPVLKVTYKVDATRVEQRTDFDKLILDVETKN SISPRDALASAGKTLVELFGLARELNVEAEGI C:  SNNSVPGAPNRVSFAKLREPLEVPGLLDVQTDSFEWLIGS PRWRESAAERGDVNPVGGLEEVLYELSPIEDFSGSMSLSF SDPRFDDVKAPVDECKDKDMTYAAPLFVTAEFINNNTGEI KSQTVFMGDFPMMTEKGTFIINGTERVVVSQLVRSPGVYF DETIDKSTDKTLHSVKVIPSRGAWLEFDVDKRDTVGVRID RKRRQPVTVLLKALGWTSEQIVERFGFSEIMRSTLEKDNT VGTDEALLDIYRKLRPGEPPTKESAQTLLENLFFKEKRYD LARVGRYKVNKKLGLHVGEPITSSTLTEEDVVATIEYLVR LHEGQTTMTVPGGVEVPVETDDIDHFGNRRLRTVGELIQN QIRVGMSRMERVVRERMTTQDVEAITPQTLINIRPVVAAI KEFFGTSQLSQFMDQNNPLSGLTHKRRLSALGPGGLSRER AGLEVRDVHPSHYGRMCPIETPEGPNIGLIGSLSVYARVN PFGFIETPYRKVVDGVVSDEIVYLTADEEDRHVVAQANSP IDADGRFVEPRVLVRRKAGEVEYVPSSEVDYMDVSPRQMV SVATAMIPFLEHDDANRALMGANMQRQAVPLVRSEAPLVG TGMELRAAIDAGDVVVAEESGVIEEVSADYITVMHDNGTR RTYRMRKFARSNHGTCANQCPIVDAGDRVEAGQVIADGPC TDDGEMALGKNLLVAIMPWEGHNYEDAIILSNRLVEEDVL TSIHIEEHEIDARDTKLGAEEITRDIPNISDEVLADLDER GIVRIGAEVRDGDILVGKVTPKGETELTPEERLLRAIFGE KAREVRDTSLKVPHGESGKVIGIRVFSREDEDELPAGVNE LVRVYVAQKRKISDGDKLAGRHGNKGVIGKILPVEDMPFL ADGTPVDIILNTHGVPRRMNIGQILETHLGWCAHSGWKVD AAKGVPDWAARLPDELLEAQPNAIVSTPVFDGAQEAELQG LLSCTLPNRDGDVLVDADGKAMLFDGRSGEPFPYPVTVGY MYIMKLHHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQR FGEMECWAMQAYGAAYTLQELLTIKSDDTVGRVKVYEAIV KGENIPEPGIPESFKVLLKELQSLCLNVEVLSSDGAAIEL REGEDE D:  DVNFFDELRIGLATAEDIRQWSYGEVKKPETINYRTLKPE KDGLFCEKIFGPTRDWECYCGKYKRVRFKGIICERCGVEV TRAKVRRERMGHIELAAPVTHIWYFKGVPSRLGYLLDLAP KDLEKIIYFAAYVITSVDEEMRHNELSTLEAEMAVERKAV EDQRDGELEARAQKLEADLAELEAEGAKADARRKVRDGGE REMRQIRDRAQRELDRLEDIWSTFTKLAPKQLIVDENLYR ELVDRYGEYFTGAMGAESIQKLIENFDIDAEAESLRDVIR NGKGQKKLRALKRLKVVAAFQQSGNSPMGMVLDAVPVIPP ELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLIDLGA PEIIVNNEKRMLQESVDALFDNGRRGRPVTGPGNRPLKSL SDLLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCG LPKLMALELFKPFVMKRLVDLNHAQNIKSAKRMVERQRPQ VWDVLEEVIAEHPVLLNRAPTLHRLGIQAFEPMLVEGKAI QLHPLVCEAFNADFDGDQMAVHLPLSAEAQAEARILMLSS NNILSPASGRPLAMPRLDMVTGLYYLTTEVPGDTGEYQPA SGDHPETGVYSSPAEAIMAADRGVLSVRAKIKVRLTQLRP PVEIEAELFGHSGWQPGDAWMAETTLGRVMFNELLPLGYP FVNKQMHKKVQAAIINDLAERYPMIVVAQTVDKLKDAGFY WATRSGVTVSMADVLVPPRKKEILDHYEERADKVEKQFQR GALNHDERNEALVEIWKEATDEVGQALREHYPDDNPIITI VDSGATGNFTQTRTLAGMKGLVTNPKGEFIPRPVKSSFRE GLTVLEYFINTHGARKGLADTALRTADSGYLTRRLVDVSQ DVIVREHDCQTERGIVVELAERAPDGTLIRDPYIETSAYA RTLGTDAVDEAGNVIVERGQDLGDPEIDALLAAGITQVKV RSVLTCATSTGVCATCYGRSMATGKLVDIGEAVGIVAAQS IGEPGTQLTGGLPRVQELFEARVPRGKAPIADVTGRVRLE DGERFYKITIVPDDGGEEVVYDKISKRQRLRVFKHEDGSE RVLSDGDHVEVGQQLMEGSADPHEVLRVQGPREVQIHLVR EVQEVYRAQGVSIHDKHIEVIVRQMLRRVTIIDSGSTEFL PGSLIDRAEFEAENRRVVAEGGEPAAGRPVLMGITKASLA TDSWLSAASFQETTRVLTDAAINCRSDKLNGLKENVIIGK LIPAGTGINRYRNIAVQPTEEARAA E:  GYDTPLGITNPPIDELLDRVSSKYALVIYAAKRARQINDY YNQLGEGILEYVGPLVEPGLQEKPLSIALREIHADLLEHT E F:  VSGAAAAEAALXRALYDEHAAVLWRYALRLTGDAAQAEDV VQETLLRAWQHPEVIGDTARPARAWLFTVARNXIIDERRS ARFRNVVGSTDQSGTPEQSTPDEVNAALDRLLIADALAQL SAEHRAVIQRSYYRGWSTAQIATDLGIAEGTVKSRLHYAV RALRLTLQELGVTR G:  GCATCCGTGAGT H:  CGTGTCAGTAACTGTCACGGATGC Description (1)  DNA-directed RNA polymerase/ECF RNA polymerase sigma factor SigL/DNA Complex Functional site 1) chain D residue 891 type sequence C description binding site for residue ZN D 2001 source : AC1 2) chain D residue 968 type sequence C description binding site for residue ZN D 2001 source : AC1 3) chain D residue 975 type sequence C description binding site for residue ZN D 2001 source : AC1 4) chain D residue 978 type sequence C description binding site for residue ZN D 2001 source : AC1 5) chain D residue 60 type sequence C description binding site for residue ZN D 2002 source : AC2 6) chain D residue 62 type sequence C description binding site for residue ZN D 2002 source : AC2 7) chain D residue 75 type sequence C description binding site for residue ZN D 2002 source : AC2 8) chain D residue 78 type sequence C description binding site for residue ZN D 2002 source : AC2 9) chain C residue 882-894 type prosite sequence GDKLAGRHGNKGV description RNA_POL_BETA RNA polymerases beta chain signature. GdKLAGrHGNKGV source prosite : PS01166 10) chain F residue 40-72 type prosite sequence AEDVVQETLLRAWQHPEVIGDTARPARAWLFTV description SIGMA70_ECF Sigma-70 factors ECF subfamily signature. AEDVVQeTLLrAWqhpevigdtarparAWLftV source prosite : PS01063 11) chain D residue 975 type DNA_BIND sequence C description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 12) chain D residue 978 type DNA_BIND sequence C description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 13) chain D residue 535 type DNA_BIND sequence D description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 14) chain D residue 537 type DNA_BIND sequence D description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 15) chain D residue 539 type DNA_BIND sequence D description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 16) chain D residue 891 type DNA_BIND sequence C description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 17) chain D residue 968 type DNA_BIND sequence C description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 18) chain D residue 62 type DNA_BIND sequence C description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 19) chain D residue 75 type DNA_BIND sequence C description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 20) chain D residue 78 type DNA_BIND sequence C description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 21) chain F residue 141-160 type DNA_BIND sequence TAQIATDLGIAEGTVKSRLH description H-T-H motif => ECO:0000255 source Swiss-Prot : SWS_FT_FI1

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