eF-site ID 6dbi-ABCD PDB Code 6dbi Chain A, B, C, D click to enlarge Title Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates Classification RECOMBINATION/DNA Compound Recombination activating gene 1 - MBP chimera Source (6DBI) Sequence A:  GGRPRQHLLSLTRRAQKHRLRDLKNQVKTFAEKEEGGDVK SVCLTLFLLALRAGNEHKQADELEAMMQGRGFGLHPAVCL AIRVNTFLSCSQYHKMYRTVKATSGRQIFQPLHTLRNAEK ELLPGFHQFEWQPALKNVSTSWDVGIIDGLSGWTVSVDDV PADTISRRFRYDVALVSALKDLEEDIMEGLRERALDDSMC TSGFTVVVKESCDGMGDVSEKHGSGPAVPEKAVRFSFTIM SISIRLEGEDDGITIFQEQKPNSELSCRPLCLMFVDESDH ETLTAILGPVVAERKAMMESRLIISVGGLLRSFRFFFRGT GYDEKMVREMEGLEASGSTYICTLCDSTRAEASQNMVLHS ITRSHDENLERYEIWRKNPFSESADELRDRVKGVSAKPFM ETQPTLDALHCDIGNATEFYKIFQDEIGEVYQKPNPSREE RRRWRSTLDKQLRKKMKLKPVMRMNGNYARRLMTREAVEA VCELVPSEERREALLKLMDLYLQMKPVWRSTCPSRDCPDQ LCQYSYNSQQFADLLSSMFKYRYDGKITNYLHKTLAHVPE IVERDGSIGAWASEGNESGNKLFRRFRKMNARQSKTFELE DILKHHWLYTSKYLQKFMEAHK B:  MSLQPLTAVNCGSLVQPGFSLLDLEGDVYLFGQKGWPKRS CPTGIFGVRIKKGELKLRAISFSNNSSYLPPLRCPAIAHF EAQDGKPECYLIHGGRTPNNELSSSLYMLSVDSRGCNRKV TLRCEEKELVGDVPSARYGHTLSVINSRGKTACVLFGGRS YMPPTERTTQNWNSVVDCPPQVYLIDLEFGCCTAHTLPEL TDGQSFHVALARQDCVYFLGGHILSSDCRPSRLIRLHVEL LLGSPVLTCTILHEGLTITSAIASPIGYHEYIIFGGYQSE TQKRMECTYVGLDDVGVHMESREPPQWTSEISHSRTWFGG SLGKGTALVAIPSEGNPTPPEAYHFYQVSFQ C:  GGRPRQHLLSLTRRAQKHRLRDLKNQVKTFAEKEEGGDVK SVCLTLFLLALRAGNEHKQADELEAMMQGRGFGLHPAVCL AIRVNTFLSCSQYHKMYRTVKATSGRQIFQPLHTLRNAEK ELLPGFHQFEWQPALKNVSTSWDVGIIDGLSGWTVSVDDV PADTISRRFRYDVALVSALKDLEEDIMEGLRERALDDSMC TSGFTVVVKESCDGMGDVSEKHGSGPAVPEKAVRFSFTIM SISIRLEGEDDGITIFQEQKPNSELSCRPLCLMFVDESDH ETLTAILGPVVAERKAMMESRLIISVGGLLRSFRFFFRGT GYDEKMVREMEGLEASGSTYICTLCDSTRAEASQNMVLHS ITRSHDENLERYEIWRKNPFSESADELRDRVKGVSAKPFM ETQPTLDALHCDIGNATEFYKIFQDEIGEVYQKPNPSREE RRRWRSTLDKQLRKKMKLKPVMRMNGNYARRLMTREAVEA VCELVPSEERREALLKLMDLYLQMKPVWRSTCPSRDCPDQ LCQYSYNSQQFADLLSSMFKYRYDGKITNYLHKTLAHVPE IVERDGSIGAWASEGNESGNKLFRRFRKMNARQSKTFELE DILKHHWLYTSKYLQKFMEAHK D:  MSLQPLTAVNCGSLVQPGFSLLDLEGDVYLFGQKGWPKRS CPTGIFGVRIKKGELKLRAISFSNNSSYLPPLRCPAIAHF EAQDGKPECYLIHGGRTPNNELSSSLYMLSVDSRGCNRKV TLRCEEKELVGDVPSARYGHTLSVINSRGKTACVLFGGRS YMPPTERTTQNWNSVVDCPPQVYLIDLEFGCCTAHTLPEL TDGQSFHVALARQDCVYFLGGHILSSDCRPSRLIRLHVEL LLGSPVLTCTILHEGLTITSAIASPIGYHEYIIFGGYQSE TQKRMECTYVGLDDVGVHMESREPPQWTSEISHSRTWFGG SLGKGTALVAIPSEGNPTPPEAYHFYQVSFQ Description Functional site 1) chain C residue 749 type sequence C description binding site for residue ZN C 1101 source : AC1 2) chain C residue 752 type sequence C description binding site for residue ZN C 1101 source : AC1 3) chain C residue 959 type sequence H description binding site for residue ZN C 1101 source : AC1 4) chain C residue 964 type sequence H description binding site for residue ZN C 1101 source : AC1 5) chain C residue 684 type sequence E description binding site for residue CA C 1102 source : AC2 6) chain C residue 730 type sequence D description binding site for residue CA C 1102 source : AC2 7) chain C residue 620 type sequence D description binding site for residue CA C 1103 source : AC3 8) chain C residue 621 type sequence G description binding site for residue CA C 1103 source : AC3 9) chain A residue 749 type sequence C description binding site for residue ZN A 1101 source : AC4 10) chain A residue 752 type sequence C description binding site for residue ZN A 1101 source : AC4 11) chain A residue 959 type sequence H description binding site for residue ZN A 1101 source : AC4 12) chain A residue 964 type sequence H description binding site for residue ZN A 1101 source : AC4 13) chain A residue 684 type sequence E description binding site for residue CA A 1102 source : AC5 14) chain A residue 620 type sequence D description binding site for residue CA A 1103 source : AC6 15) chain A residue 730 type sequence D description binding site for residue CA A 1103 source : AC6 16) chain C residue 408-475 type DNA_BIND sequence GGRPRQHLLSLTRRAQKHRLRDLKNQVKTFAEKEEGGDVK SVCLTLFLLALRAGNEHKQADELEAMMQ description NBD => ECO:0000255|PROSITE-ProRule:PRU00820 source Swiss-Prot : SWS_FT_FI3 17) chain A residue 408-475 type DNA_BIND sequence GGRPRQHLLSLTRRAQKHRLRDLKNQVKTFAEKEEGGDVK SVCLTLFLLALRAGNEHKQADELEAMMQ description NBD => ECO:0000255|PROSITE-ProRule:PRU00820 source Swiss-Prot : SWS_FT_FI3 18) chain C residue 620 type BINDING sequence D description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI4 19) chain C residue 730 type BINDING sequence D description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI4 20) chain C residue 984 type BINDING sequence E description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI4 21) chain A residue 620 type BINDING sequence D description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI4 22) chain A residue 730 type BINDING sequence D description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI4 23) chain A residue 984 type BINDING sequence E description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI4 24) chain C residue 915 type SITE sequence W description Essential for DNA hairpin formation, participates in base-stacking interactions near the cleavage site => ECO:0000250 source Swiss-Prot : SWS_FT_FI6 25) chain A residue 915 type SITE sequence W description Essential for DNA hairpin formation, participates in base-stacking interactions near the cleavage site => ECO:0000250 source Swiss-Prot : SWS_FT_FI6

Display surface Download Links