eF-site/Summary 6a95-A

eF-site ID	6a95-A
PDB Code	6a95
Chain	A

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Title	Complex of voltage-gated sodium channel NavPaS from American cockroach Periplaneta americana bound with tetrodotoxin and Dc1a
Classification	MEMBRANE PROTEIN/TOXIN
Compound	Sodium channel protein PaFPC1
Source	(TXI92_DIGCA)

Sequence	A:	DKGRGANKDRDGSAHPDQALEQGSRLPARMRNIFPAELAS TPLEDFDPFYKNKKTFVVVTKAGDIFRFSGEKSLWMLDPF TPIRRVAISTMVQPIFSYFIMITILIHCIFMIMPATQTTY ILELVFLSIYTIEVVVKVLARGFILHPFAYLRDPWNWLDF LVTLIGYITLVVDLGHLYALRAFRVLRSWRTVTIVPGWRT IVDALSLSITSLKDLVLLLLFSLFVFAVLGLQIYMGVLTQ KCVKHFPADGSWGNFTDERWFNYTSNSSHWYIPDDWIEYP LCGNSSGAGMCPPGYTCLQGYGGNPNYGYTSFDTFGWAFL SVFRLVTLDYWEDLYQLALRSAGPWHILFFIIVVFYGTFC FLNFILAVVVMSYTHMVKRADEEKAAEREQGAIGAVVLSP FFELFIAVIIVLNITFMALDHHDMNIEFERILRTGNYIFT SIYIVEAVLKIIALSPKFYFKDSWNVFDFIIVVFAILELG LEGVQGLSVFRSFRLLRVFRLAKFWPTLNNFMSVMTKSYG AFVNVMYVMFLLLFIFAIIGMQLFGMNYIDNMERFPDGDL PRWNFTDFLHSFMIVFRALCGEWIESMWDCMLVGDWSCIP FFVAVFFVGNLVILNLLIALLLNNYRMWSNIRRVCFLLAK NKYFQKFVTAVLVITSVLLALEDIYLPQRPVLVNITLYVD YVLTAFFVIEMIIMLFAVGFKKYFTSKWYWLDFIVVVAYL LNFVLMCAGIEALQTLRLLRVFRLFRPLSKVNGMQVVTST LVEAVPHIFNVILVGIFFWLVFAIMGVQLFAGKFYKCVDE NSTVLSHEITMDRNDCLHENYTWENSPMNFDHVGNAYLSL LQVATFKGWLQIMNDAIDSREVHKQPIRETNIYMYLYFIF FIVFGSFFILKLFVCILIDIFRQQRRKAEGLSATDSRTQL IYRRAVMRTMSAKPVKRIPKPTCHPQSLMYDISVNRKFEY TMMILIILNVAVMAIDHYGQSMEFSEVLDYLNLIFIIIFF VECVIKVSGLRHHYFKDPWNIIDFLYVVLAIAGLMLSDVI EKYFISPTLLRILRILRVGRLLRYFQSARGMRLLLLALRK ALRTLFNVSFLLFVIMFVYAVFGMEFFMHIRDAGAIDDVY NFKTFGQSIILLFQLATSAGWDGVYFAIANEEDCRAPDHE LGYPGNCGSRALGIAYLVSYLIITCLVVINMYAAVILDYV LEVYEDSKEGLTDDDYDMFFEVWQQFDPEATQYIRYDQLS ELLEALQPPLQVQKPNKYKILSMNIPICKDDHIFYKDVLE ALVKDVFSRRGSPVEAGDVQAPNVDEA

Description	(1)	Sodium channel protein PaFPC1, Mu-diguetoxin-Dc1a

Functional site


1)	chain	A
	residue	1109-1171
	type	SITE
	sequence	CILIDIFRQQRRKAEGLSATDSRTQLIYRRAVMRTMSAKP VKRIPKPTCHPQSLMYDISVNRK
	description	Interacts with insect Nav channel => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI1

2)	chain	A
	residue	1220-1231
	type	SITE
	sequence	KVSGLRHHYFKD
	description	Interacts with insect Nav channel => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI1

3)	chain	A
	residue	1280-1298
	type	SITE
	sequence	QSARGMRLLLLALRKALRT
	description	Interacts with insect Nav channel => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI1

4)	chain	A
	residue	908-921
	type	SITE
	sequence	MLFAVGFKKYFTSK
	description	Interacts with insect Nav channel => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI1

5)	chain	A
	residue	965-981
	type	SITE
	sequence	VNGMQVVTSTLVEAVPH
	description	Interacts with insect Nav channel => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI1

6)	chain	A
	residue	520-538
	type	TRANSMEM
	sequence	FFELFIAVIIVLNITFMAL
	description	Helical; Name=S1 of repeat II => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI10

7)	chain	A
	residue	858-875
	type	TRANSMEM
	sequence	FQKFVTAVLVITSVLLAL
	description	Helical; Name=S1 of repeat III => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI16

8)	chain	A
	residue	889-907
	type	TRANSMEM
	sequence	ITLYVDYVLTAFFVIEMII
	description	Helical; Name=S2 of repeat III => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI17

9)	chain	A
	residue	922-940
	type	TRANSMEM
	sequence	WYWLDFIVVVAYLLNFVLM
	description	Helical; Name=S3 of repeat III => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI18

10)	chain	A
	residue	550-569
	type	TRANSMEM
	sequence	ILRTGNYIFTSIYIVEAVLK
	description	Helical; Name=S2 of repeat II => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI11

11)	chain	A
	residue	584-603
	type	TRANSMEM
	sequence	NVFDFIIVVFAILELGLEGV
	description	Helical; Name=S3 of repeat II => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI12

12)	chain	A
	residue	606-623
	type	TRANSMEM
	sequence	LSVFRSFRLLRVFRLAKF
	description	Helical; Name=S4 of repeat II => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI13

13)	chain	A
	residue	640-658
	type	TRANSMEM
	sequence	AFVNVMYVMFLLLFIFAII
	description	Helical; Name=S5 of repeat II => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI14

14)	chain	A
	residue	720-740
	type	TRANSMEM
	sequence	FFVAVFFVGNLVILNLLIALL
	description	Helical; Name=S6 of repeat II => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI15

15)	chain	A
	residue	946-964
	type	TRANSMEM
	sequence	ALQTLRLLRVFRLFRPLSK
	description	Helical; Name=S4 of repeat III => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI19

16)	chain	A
	residue	141-159
	type	TRANSMEM
	sequence	IFSYFIMITILIHCIFMIM
	description	Helical; Name=S1 of repeat I => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI2

17)	chain	A
	residue	982-1001
	type	TRANSMEM
	sequence	IFNVILVGIFFWLVFAIMGV
	description	Helical; Name=S5 of repeat III => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI20

18)	chain	A
	residue	1087-1108
	type	TRANSMEM
	sequence	YMYLYFIFFIVFGSFFILKLFV
	description	Helical; Name=S6 of repeat III => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI21

19)	chain	A
	residue	1172-1189
	type	TRANSMEM
	sequence	FEYTMMILIILNVAVMAI
	description	Helical; Name=S1 of repeat IV => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI22

20)	chain	A
	residue	1201-1219
	type	TRANSMEM
	sequence	VLDYLNLIFIIIFFVECVI
	description	Helical; Name=S2 of repeat IV => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI23

21)	chain	A
	residue	1232-1249
	type	TRANSMEM
	sequence	PWNIIDFLYVVLAIAGLM
	description	Helical; Name=S3 of repeat IV => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI24

22)	chain	A
	residue	1263-1279
	type	TRANSMEM
	sequence	LLRILRILRVGRLLRYF
	description	Helical; Name=S4 of repeat IV => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI25

23)	chain	A
	residue	1299-1316
	type	TRANSMEM
	sequence	LFNVSFLLFVIMFVYAVF
	description	Helical; Name=S5 of repeat IV => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI26

24)	chain	A
	residue	1388-1410
	type	TRANSMEM
	sequence	IAYLVSYLIITCLVVINMYAAVI
	description	Helical; Name=S6 of repeat IV => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI27

25)	chain	A
	residue	160-165
	type	TOPO_DOM
	sequence	PATQTT
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

26)	chain	A
	residue	941-945
	type	TOPO_DOM
	sequence	CAGIE
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

27)	chain	A
	residue	1002-1047
	type	TOPO_DOM
	sequence	QLFAGKFYKCVDENSTVLSHEITMDRNDCLHENYTWENSP MNFDHV
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

28)	chain	A
	residue	1070-1086
	type	TOPO_DOM
	sequence	AIDSREVHKQPIRETNI
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

29)	chain	A
	residue	1190-1200
	type	TOPO_DOM
	sequence	DHYGQSMEFSE
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

30)	chain	A
	residue	1250-1262
	type	TOPO_DOM
	sequence	LSDVIEKYFISPT
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

31)	chain	A
	residue	1317-1338
	type	TOPO_DOM
	sequence	GMEFFMHIRDAGAIDDVYNFKT
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

32)	chain	A
	residue	1362-1387
	type	TOPO_DOM
	sequence	IANEEDCRAPDHELGYPGNCGSRALG
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

33)	chain	A
	residue	219-224
	type	TOPO_DOM
	sequence	DLGHLY
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

34)	chain	A
	residue	281-360
	type	TOPO_DOM
	sequence	MGVLTQKCVKHFPADGSWGNFTDERWFNYTSNSSHWYIPD DWIEYPLCGNSSGAGMCPPGYTCLQGYGGNPNYGYTSFDT
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

35)	chain	A
	residue	386-392
	type	TOPO_DOM
	sequence	RSAGPWH
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

36)	chain	A
	residue	539-549
	type	TOPO_DOM
	sequence	DHHDMNIEFER
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

37)	chain	A
	residue	604-605
	type	TOPO_DOM
	sequence	QG
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

38)	chain	A
	residue	659-686
	type	TOPO_DOM
	sequence	GMQLFGMNYIDNMERFPDGDLPRWNFTD
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

39)	chain	A
	residue	708-719
	type	TOPO_DOM
	sequence	DCMLVGDWSCIP
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

40)	chain	A
	residue	876-888
	type	TOPO_DOM
	sequence	EDIYLPQRPVLVN
	description	Extracellular => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI3

41)	chain	A
	residue	166-186
	type	TRANSMEM
	sequence	YILELVFLSIYTIEVVVKVLA
	description	Helical; Name=S2 of repeat I => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI4

42)	chain	A
	residue	201-218
	type	TRANSMEM
	sequence	WNWLDFLVTLIGYITLVV
	description	Helical; Name=S3 of repeat I => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI5

43)	chain	A
	residue	225-241
	type	TRANSMEM
	sequence	ALRAFRVLRSWRTVTIV
	description	Helical; Name=S4 of repeat I => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI6

44)	chain	A
	residue	261-280
	type	TRANSMEM
	sequence	LVLLLLFSLFVFAVLGLQIY
	description	Helical; Name=S5 of repeat I => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI7

45)	chain	A
	residue	378
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:6A91
	source	Swiss-Prot : SWS_FT_FI28

46)	chain	A
	residue	1063
	type	BINDING
	sequence	W
	description	BINDING => ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:6A91
	source	Swiss-Prot : SWS_FT_FI28

47)	chain	A
	residue	701
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI29

48)	chain	A
	residue	704
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI29

49)	chain	A
	residue	1062
	type	BINDING
	sequence	G
	description	BINDING => ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI29

50)	chain	A
	residue	1354
	type	BINDING
	sequence	G
	description	BINDING => ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI29

51)	chain	A
	residue	1356
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI29

52)	chain	A
	residue	539
	type	SITE
	sequence	D
	description	Interacts with the spider Mu-diguetoxin-Dc1a => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI30

53)	chain	A
	residue	542
	type	SITE
	sequence	D
	description	Interacts with the spider Mu-diguetoxin-Dc1a => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI30

54)	chain	A
	residue	549
	type	SITE
	sequence	R
	description	Interacts with the spider Mu-diguetoxin-Dc1a => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI30

55)	chain	A
	residue	613
	type	SITE
	sequence	R
	description	Interacts with the spider Mu-diguetoxin-Dc1a => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI30

56)	chain	A
	residue	1002
	type	SITE
	sequence	Q
	description	Interacts with the spider Mu-diguetoxin-Dc1a => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI30

57)	chain	A
	residue	1027
	type	SITE
	sequence	R
	description	Interacts with the spider Mu-diguetoxin-Dc1a => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI30

58)	chain	A
	residue	1032
	type	SITE
	sequence	H
	description	Interacts with the spider Mu-diguetoxin-Dc1a => ECO:0000269\|PubMed:30049784
	source	Swiss-Prot : SWS_FT_FI30

59)	chain	A
	residue	300
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI31

60)	chain	A
	residue	308
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:28183995, ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:5X0M, ECO:0007744\|PDB:6A90, ECO:0007744\|PDB:6A91, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI32

61)	chain	A
	residue	312
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:28183995, ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:5X0M, ECO:0007744\|PDB:6A90, ECO:0007744\|PDB:6A91, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI32

62)	chain	A
	residue	330
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:28183995, ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:5X0M, ECO:0007744\|PDB:6A90, ECO:0007744\|PDB:6A91, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI32

63)	chain	A
	residue	1015
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:28183995, ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:5X0M, ECO:0007744\|PDB:6A90, ECO:0007744\|PDB:6A91, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI32

64)	chain	A
	residue	1034
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:28183995, ECO:0000269\|PubMed:30049784, ECO:0007744\|PDB:5X0M, ECO:0007744\|PDB:6A90, ECO:0007744\|PDB:6A91, ECO:0007744\|PDB:6A95
	source	Swiss-Prot : SWS_FT_FI32

65)	chain	A
	residue	683
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000255\|PROSITE-ProRule:PRU00498
	source	Swiss-Prot : SWS_FT_FI33

66)	chain	A
	residue	1028
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine; atypical => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI34

67)	chain	A
	residue	361-385
	type	INTRAMEM
	sequence	FGWAFLSVFRLVTLDYWEDLYQLAL
	description	Pore-forming => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI8

68)	chain	A
	residue	687-707
	type	INTRAMEM
	sequence	FLHSFMIVFRALCGEWIESMW
	description	Pore-forming => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI8

69)	chain	A
	residue	1048-1069
	type	INTRAMEM
	sequence	GNAYLSLLQVATFKGWLQIMND
	description	Pore-forming => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI8

70)	chain	A
	residue	1339-1361
	type	INTRAMEM
	sequence	FGQSIILLFQLATSAGWDGVYFA
	description	Pore-forming => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI8

71)	chain	A
	residue	393-413
	type	TRANSMEM
	sequence	ILFFIIVVFYGTFCFLNFILA
	description	Helical; Name=S6 of repeat I => ECO:0000269\|PubMed:28183995, ECO:0007744\|PDB:5X0M
	source	Swiss-Prot : SWS_FT_FI9