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eF-site ID
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5x21-C |
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PDB Code
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5x21 |
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Chain
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C |
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click to enlarge
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Title
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Crystal structure of Thermus thermophilus transcription initiation complex with GpA and pseudouridimycin (PUM) |
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Classification
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TRANSFERASE/DNA |
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Compound
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DNA-directed RNA polymerase subunit alpha |
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Source
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ORGANISM_SCIENTIFIC: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579); |
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Sequence
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C: |
MEIKRFGRIREVIPLPPLTEIQVESYRRALQADVPPEKRE
NVGIQAAFRETFPIEELVLDFLEYRLGEPPFPQDECREKD
LTYQAPLYARLQLIHKDTGLIKEDEVFLGHIPLMTEDGSF
IINGADRVIVSQIHRSPGVYFTPDPARPGRYIASIIPLPK
RGPWIDLEVEPNGVVSMKVNKRKFPLVLLLRVLGYDQETL
ARELGAYGELVQGLMDESVFAMRPEEALIRLFTLLRPGDP
PKRDKAVAYVYGLIADPRRYDLGEAGRYKAEEKLGIRLSG
RTLARFEDGEFKDEVFLPTLRYLFALTAGVPGHEVDDIDH
LGNRRIRTVGELMTDQFRVGLARLARGVRERMLMGSEDSL
TPAKLVNSRPLEAAIREFFSRSQLSQFKDETNPLSSLRHK
RRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGA
NIGLITSLAAYARVDELGFIRTPYRRVVGGVVTDEVVYMT
ATEEDRYTIAQANTPLEGNRIAAERVVARRKGEPVIVSPE
EVEFMDVSPKQVFSVNTNLIPFLEHDDANRALMGSNMQTQ
AVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVAKVD
GNRIVVRYEDGRLVEYPLRRFYRSNQGTALDQRPRVVVGQ
RVRKGDLLADGPASENGFLALGQNVLVAIMPFDGYNFEDA
IVISEELLKRDFYTSIHIERYEIEARDTKLGPERITRDIP
HLSEAALRDLDEEGVVRIGAEVKPGDILVGRTSFKGESEP
TPEERLLRSIFGEKARDVKDTSLRVPPGEGGIVVRTVRLR
RGDPGVELKPGVREVVRVYVAQKRKLQVGDKLANRHGNKG
VVAKILPVEDMPHLPDGTPVDVILNPLGVPSRMNLGQILE
THLGLAGYFLGQRYISPIFDGAKEPEIKELLAQAFEVYFG
KRKGEGFGVDKREVEVLRRAEKLGLVTPGKTPEEQLKELF
LQGKVVLYDGRTGEPIEGPIVVGQMFIMKLYHMVEDKMHA
RSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAH
TLQEMLTLKSDDIEGRNAAYEAIIKGEDVPEPSVPESFRV
LVKELQALALDVQTLDEKDNPVDIFEGLASK
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Description
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Functional site
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1)
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chain |
C |
| residue |
387 |
| type |
|
| sequence |
S
|
| description |
binding site for residue MG C 2000
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| source |
: AC2
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2)
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chain |
C |
| residue |
389 |
| type |
|
| sequence |
S
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| description |
binding site for residue MG C 2000
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| source |
: AC2
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3)
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chain |
C |
| residue |
392 |
| type |
|
| sequence |
S
|
| description |
binding site for residue MG C 2000
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| source |
: AC2
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4)
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chain |
C |
| residue |
445 |
| type |
|
| sequence |
E
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| description |
binding site for residue PUM I 101
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| source |
: AC9
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5)
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chain |
C |
| residue |
846 |
| type |
|
| sequence |
K
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| description |
binding site for residue PUM I 101
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| source |
: AC9
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6)
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chain |
C |
| residue |
836-848 |
| type |
prosite |
| sequence |
GDKLANRHGNKGV
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| description |
RNA_POL_BETA RNA polymerases beta chain signature. GdKLANrHGNKGV
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| source |
prosite : PS01166
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