eF-site ID 5mga-ABCD PDB Code 5mga Chain A, B, C, D click to enlarge Title Structure of the Cpf1 endonuclease R-loop complex after DNA cleavage Classification HYDROLASE Compound CRISPR-associated endonuclease Cpf1 Source (5MGA) Sequence A:  IYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEK RAKDYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDV YFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFKNL FNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDI DEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYR IVDDNLPKFLENKAKYESLKDKAPYEQIKKDLAEELTFDD DYKNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFV NGENTKRKGINEYINLYSQNDKTLKKYKMSVLFKQILSDT ESKSFVIDKLEDDSDVVTTMQSFYEQIAAFKTKETLSLLF DDLKKSLTDLSQQVFDDYKEQELIAKKTEKAKYLSLETIL IDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQN QGKKDLLQASAEDDVKAIKDLLDQTNNLLHKLKIFHISQS EDKADEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSD EKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVM NKKNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVF FSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFN IEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDF SAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYR KQSIPKKIIANKNKDNPKKESVFEYDLIKDKRFTEDKFFF HCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGE RHLAYYTLVDGKGNIIKQDTFNIIGNYHDKLAAIEKDRDS ARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFE DLNFGVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLR AYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVN QLYPVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKG KWTIASFGSRLINNHNWDTREVYPTKELEKLLKDYGECIK AICGESDKKFFAKLTSVLNTILQMRNSKELDYLISPVADV NGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNN QEKLNLVIKNEEYFEFVQNRNN B:  AAUUUCUACUGUUGUAGAUGAGAAGUCAUUUAAUAAGGCC C:  GGCCTTATTAAATGACTTCTCTAACG D:  CGTTAGAGAAGT Description (1)  CRISPR-associated endonuclease Cpf1/RNA Complex Functional site 1) chain A residue 802 type sequence N description binding site for residue MG B 101 source : AC1 2) chain B residue -4 type sequence A description binding site for residue MG B 101 source : AC1 3) chain B residue -3 type sequence G description binding site for residue MG B 101 source : AC1 4) chain B residue -5 type sequence U description binding site for residue MG B 102 source : AC2 5) chain B residue -8 type sequence U description binding site for residue MG B 102 source : AC2 6) chain A residue 852 type ACT_SITE sequence K description For pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI1 7) chain A residue 869 type ACT_SITE sequence K description For pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI1 8) chain A residue 671 type SITE sequence K description Binds PAM => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI10 9) chain A residue 852 type SITE sequence K description Stabilizes transition state for pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI14 10) chain A residue 1026 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI15 11) chain A residue 1063 type SITE sequence T description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI15 12) chain A residue 677 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI11 13) chain A residue 823 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI11 14) chain A residue 826 type SITE sequence G description Binds Target strand DNA; via amide nitrogen => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI12 15) chain A residue 833 type SITE sequence R description Binds crRNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI13 16) chain A residue 917 type ACT_SITE sequence D description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 17) chain A residue 1006 type ACT_SITE sequence E description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 18) chain A residue 1255 type ACT_SITE sequence D description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 19) chain A residue 16 type SITE sequence T description Binds crRNA alone and in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI3 20) chain A residue 131 type SITE sequence K description Binds target strand DNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI4 21) chain A residue 295 type SITE sequence T description Binds crRNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI5 22) chain A residue 320 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 23) chain A residue 334 type SITE sequence S description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 24) chain A residue 589 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 25) chain A residue 410 type SITE sequence Y description Caps the crRNA-target DNA heteroduplex => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI7 26) chain A residue 613 type SITE sequence K description Binds DNA protospacer adjacent motif (PAM) => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI8 27) chain A residue 667 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI9

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