eF-site ID 5mga-A PDB Code 5mga Chain A click to enlarge Title Structure of the Cpf1 endonuclease R-loop complex after DNA cleavage Classification HYDROLASE Compound CRISPR-associated endonuclease Cpf1 Source (5MGA) Sequence A:  IYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEK RAKDYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDV YFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFKNL FNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDI DEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYR IVDDNLPKFLENKAKYESLKDKAPYEQIKKDLAEELTFDD DYKNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFV NGENTKRKGINEYINLYSQNDKTLKKYKMSVLFKQILSDT ESKSFVIDKLEDDSDVVTTMQSFYEQIAAFKTKETLSLLF DDLKKSLTDLSQQVFDDYKEQELIAKKTEKAKYLSLETIL IDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQN QGKKDLLQASAEDDVKAIKDLLDQTNNLLHKLKIFHISQS EDKADEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSD EKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVM NKKNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVF FSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFN IEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDF SAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYR KQSIPKKIIANKNKDNPKKESVFEYDLIKDKRFTEDKFFF HCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGE RHLAYYTLVDGKGNIIKQDTFNIIGNYHDKLAAIEKDRDS ARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFE DLNFGVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLR AYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVN QLYPVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKG KWTIASFGSRLINNHNWDTREVYPTKELEKLLKDYGECIK AICGESDKKFFAKLTSVLNTILQMRNSKELDYLISPVADV NGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNN QEKLNLVIKNEEYFEFVQNRNN Description (1)  CRISPR-associated endonuclease Cpf1/RNA Complex Functional site 1) chain A residue 802 type sequence N description binding site for residue MG B 101 source : AC1 2) chain A residue 852 type ACT_SITE sequence K description For pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI1 3) chain A residue 869 type ACT_SITE sequence K description For pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI1 4) chain A residue 671 type SITE sequence K description Binds PAM => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI10 5) chain A residue 852 type SITE sequence K description Stabilizes transition state for pre-crRNA processing => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI14 6) chain A residue 1026 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI15 7) chain A residue 1063 type SITE sequence T description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI15 8) chain A residue 677 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI11 9) chain A residue 823 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI11 10) chain A residue 826 type SITE sequence G description Binds Target strand DNA; via amide nitrogen => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI12 11) chain A residue 833 type SITE sequence R description Binds crRNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI13 12) chain A residue 917 type ACT_SITE sequence D description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 13) chain A residue 1006 type ACT_SITE sequence E description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 14) chain A residue 1255 type ACT_SITE sequence D description For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 source Swiss-Prot : SWS_FT_FI2 15) chain A residue 16 type SITE sequence T description Binds crRNA alone and in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI3 16) chain A residue 131 type SITE sequence K description Binds target strand DNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI4 17) chain A residue 295 type SITE sequence T description Binds crRNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI5 18) chain A residue 320 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 19) chain A residue 334 type SITE sequence S description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 20) chain A residue 589 type SITE sequence K description Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI6 21) chain A residue 410 type SITE sequence Y description Caps the crRNA-target DNA heteroduplex => ECO:0000305|PubMed:28431230 source Swiss-Prot : SWS_FT_FI7 22) chain A residue 613 type SITE sequence K description Binds DNA protospacer adjacent motif (PAM) => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 source Swiss-Prot : SWS_FT_FI8 23) chain A residue 667 type SITE sequence K description Binds Target strand DNA => ECO:0000269|PubMed:28431230 source Swiss-Prot : SWS_FT_FI9

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