eF-site ID 5lmm-L PDB Code 5lmm Chain L click to enlarge Title Structure of E coli Hydrogenase Hyd-1 mutant E28Q Classification OXIDOREDUCTASE Compound Hydrogenase-1 small chain Source ORGANISM_SCIENTIFIC: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC); Sequence L:  STQYETQGYTINNAGRRLVVDPITRIQGHMRCEVNINDQN VITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVXTG VHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHLV HFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPG YFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLM GFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCAI NIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALA IGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLM PGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYRYPNDQV GRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAP RWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLP LSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNGN LATASTEKWEPATWPTECRGVGFTEAPRGALGHWAAIRDG KIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPE QPLEILRTLHSFDPCLACSTH Description Functional site 1) chain L residue 226 type sequence K description binding site for residue F3S S 402 source : AC2 2) chain L residue 482 type sequence L description binding site for residue SO4 L 601 source : AC7 3) chain L residue 483 type sequence A description binding site for residue SO4 L 601 source : AC7 4) chain L residue 79 type sequence X description binding site for residue FCO L 602 source : AC8 5) chain L residue 82 type sequence V description binding site for residue FCO L 602 source : AC8 6) chain L residue 83 type sequence H description binding site for residue FCO L 602 source : AC8 7) chain L residue 507 type sequence A description binding site for residue FCO L 602 source : AC8 8) chain L residue 508 type sequence P description binding site for residue FCO L 602 source : AC8 9) chain L residue 509 type sequence R description binding site for residue FCO L 602 source : AC8 10) chain L residue 512 type sequence L description binding site for residue FCO L 602 source : AC8 11) chain L residue 530 type sequence V description binding site for residue FCO L 602 source : AC8 12) chain L residue 531 type sequence P description binding site for residue FCO L 602 source : AC8 13) chain L residue 532 type sequence T description binding site for residue FCO L 602 source : AC8 14) chain L residue 579 type sequence C description binding site for residue FCO L 602 source : AC8 15) chain L residue 76 type sequence C description binding site for residue NI L 603 source : AC9 16) chain L residue 79 type sequence X description binding site for residue NI L 603 source : AC9 17) chain L residue 576 type sequence C description binding site for residue NI L 603 source : AC9 18) chain L residue 579 type sequence C description binding site for residue NI L 603 source : AC9 19) chain L residue 57 type sequence E description binding site for residue MG L 604 source : AD1 20) chain L residue 528 type sequence C description binding site for residue MG L 604 source : AD1 21) chain L residue 582 type sequence H description binding site for residue MG L 604 source : AD1 22) chain L residue 97 type sequence I description binding site for residue LI L 605 source : AD2 23) chain L residue 76 type BINDING sequence C description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 24) chain L residue 79 type BINDING sequence X description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 25) chain L residue 576 type BINDING sequence C description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 26) chain L residue 579 type BINDING sequence C description BINDING => ECO:0000255 source Swiss-Prot : SWS_FT_FI1 27) chain L residue 54-79 type prosite sequence RGLEIILQGRDPRDAWAFVERICGVX description NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEiilqgrdprdawafvERiCGVC source prosite : PS00507 28) chain L residue 573-582 type prosite sequence FDPCLACSTH description NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H source prosite : PS00508

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