eF-site ID 5fw2-B PDB Code 5fw2 Chain B click to enlarge Title Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA Classification HYDROLASE/DNA/RNA Compound SGRNA Source (5FW2) Sequence B:  KKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHS IKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYL QEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMI KFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMI KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGY IDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVV DKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYN ELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVK QLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIK DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHL FDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVDSLHEHIAN LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARK NSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYY LQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNK VLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQR KFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQIL DSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKV REINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDGKA TAKYFFYSNIMNFFKTEITKRPLIETNGETGEIVWDKGRD FATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKL IARKKDWDPKKYGGFESPTVAYSVLVVAKVEKGKSKKLKS VKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLP KYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLA SHNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDK VLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDT TIDRKQYRSTKEVLDATLIHQSITGLYETRIDLSQL Description Functional site 1) chain B residue 1350 type sequence Q description BINDING SITE FOR RESIDUE K B 2366 source : AC4 2) chain B residue 584 type sequence E description BINDING SITE FOR RESIDUE K B 2367 source : AC5 3) chain B residue 586 type sequence R description BINDING SITE FOR RESIDUE K B 2367 source : AC5 4) chain B residue 588 type sequence N description BINDING SITE FOR RESIDUE K B 2367 source : AC5 5) chain B residue 1102 type sequence T description BINDING SITE FOR RESIDUE K B 2368 source : AC6 6) chain B residue 623 type sequence L description BINDING SITE FOR RESIDUE K B 2369 source : AC7 7) chain B residue 624 type sequence T description BINDING SITE FOR RESIDUE K B 2369 source : AC7 8) chain B residue 626 type sequence F description BINDING SITE FOR RESIDUE K B 2369 source : AC7 9) chain B residue 656 type sequence Y description BINDING SITE FOR RESIDUE K B 2369 source : AC7 10) chain B residue 361 type sequence G description BINDING SITE FOR RESIDUE K B 2370 source : AC8 11) chain B residue 362 type sequence Y description BINDING SITE FOR RESIDUE K B 2370 source : AC8 12) chain B residue 365 type sequence G description BINDING SITE FOR RESIDUE K B 2370 source : AC8 13) chain B residue 366 type sequence G description BINDING SITE FOR RESIDUE K B 2370 source : AC8 14) chain B residue 367 type sequence A description BINDING SITE FOR RESIDUE K B 2370 source : AC8 15) chain B residue 640 type sequence A description BINDING SITE FOR RESIDUE K B 2371 source : AC9 16) chain B residue 643 type sequence F description BINDING SITE FOR RESIDUE K B 2371 source : AC9 17) chain B residue 1102 type sequence T description BINDING SITE FOR RESIDUE K B 2372 source : BC1 18) chain B residue 111 type sequence K description BINDING SITE FOR RESIDUE K B 2373 source : BC2 19) chain B residue 10 type ACT_SITE sequence A description For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477 source Swiss-Prot : SWS_FT_FI1 20) chain B residue 840 type ACT_SITE sequence A description Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477 source Swiss-Prot : SWS_FT_FI2 21) chain B residue 10 type BINDING sequence A description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 22) chain B residue 762 type BINDING sequence E description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 23) chain B residue 983 type BINDING sequence H description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 24) chain B residue 1297 type BINDING sequence H description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 25) chain B residue 1328 type BINDING sequence D description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3

Display surface Download Links