eF-site ID 5b2s-B PDB Code 5b2s Chain B click to enlarge Title Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM) Classification HYDROLASE/RNA/DNA Compound Guide RNA Source null (5B2S) Sequence B:  KYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSI KKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRILYLQ EIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIV DEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNLFIQLVQTYNQLFEENPINASGVDA KAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGL TPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYAD LFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEH HQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGAS QEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNG SIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRI PYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASA QSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVK YVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIEEFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLD NEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGF ANRNFMQLIHDDSLTFKEDIQKAQDSLHEHIANLAGSPAI KKGILQTVKVVDELVKVMGRHKPENIVIEMARNSRERMKR IEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMY VDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKA ERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKY DENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHH AHDAYLNAVVGTALIKKYPKLESEFVYGKATAKYFFYSNI MNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFAT VRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIAR KKDWDPKKYGGFESPTVAYSVLVVAKVEKGKSKKLKSVKE LLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHY EPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANL DKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYF DTTIDRKQYRSTKEVLDATLIHQSITGLYETRIDLSQLG Description Functional site 1) chain B residue 1102 type sequence T description binding site for residue K A 101 source : AC1 2) chain B residue 1350 type sequence Q description binding site for residue K A 102 source : AC2 3) chain B residue 461 type sequence R description binding site for residue EDO A 106 source : AC6 4) chain B residue 1102 type sequence T description binding site for residue ACT A 107 source : AC7 5) chain B residue 1171 type sequence R description binding site for residue ACT A 107 source : AC7 6) chain B residue 623 type sequence L description binding site for residue K B 1401 source : AC8 7) chain B residue 624 type sequence T description binding site for residue K B 1401 source : AC8 8) chain B residue 626 type sequence F description binding site for residue K B 1401 source : AC8 9) chain B residue 656 type sequence Y description binding site for residue K B 1401 source : AC8 10) chain B residue 584 type sequence E description binding site for residue K B 1402 source : AC9 11) chain B residue 586 type sequence R description binding site for residue K B 1402 source : AC9 12) chain B residue 588 type sequence N description binding site for residue K B 1402 source : AC9 13) chain B residue 226 type sequence I description binding site for residue K B 1403 source : AD1 14) chain B residue 229 type sequence L description binding site for residue K B 1403 source : AD1 15) chain B residue 232 type sequence E description binding site for residue K B 1403 source : AD1 16) chain B residue 361 type sequence G description binding site for residue K B 1404 source : AD2 17) chain B residue 362 type sequence Y description binding site for residue K B 1404 source : AD2 18) chain B residue 365 type sequence G description binding site for residue K B 1404 source : AD2 19) chain B residue 367 type sequence A description binding site for residue K B 1404 source : AD2 20) chain B residue 364 type sequence D description binding site for residue K B 1405 source : AD3 21) chain B residue 365 type sequence G description binding site for residue K B 1405 source : AD3 22) chain B residue 108 type sequence E description binding site for residue K B 1406 source : AD4 23) chain B residue 109 type sequence E description binding site for residue K B 1406 source : AD4 24) chain B residue 111 type sequence K description binding site for residue K B 1406 source : AD4 25) chain B residue 57 type sequence E description binding site for residue K B 1407 source : AD5 26) chain B residue 576 type sequence D description binding site for residue K B 1408 source : AD6 27) chain B residue 577 type sequence S description binding site for residue K B 1408 source : AD6 28) chain B residue 640 type sequence A description binding site for residue K B 1408 source : AD6 29) chain B residue 643 type sequence F description binding site for residue K B 1408 source : AD6 30) chain B residue 1105 type sequence F description binding site for residue EDO B 1410 source : AD8 31) chain B residue 1168 type sequence I description binding site for residue EDO B 1410 source : AD8 32) chain B residue 1169 type sequence M description binding site for residue EDO B 1410 source : AD8 33) chain B residue 1171 type sequence R description binding site for residue EDO B 1410 source : AD8 34) chain B residue 1172 type sequence S description binding site for residue EDO B 1410 source : AD8 35) chain B residue 1139 type sequence V description binding site for residue EDO B 1411 source : AD9 36) chain B residue 1165 type sequence G description binding site for residue EDO B 1411 source : AD9 37) chain B residue 1167 type sequence T description binding site for residue EDO B 1411 source : AD9 38) chain B residue 136 type sequence Y description binding site for residue EDO B 1412 source : AE1 39) chain B residue 139 type sequence R description binding site for residue EDO B 1412 source : AE1 40) chain B residue 160 type sequence H description binding site for residue EDO B 1412 source : AE1 41) chain B residue 407 type sequence N description binding site for residue EDO B 1412 source : AE1 42) chain B residue 415 type sequence H description binding site for residue EDO B 1412 source : AE1 43) chain B residue 10 type ACT_SITE sequence A description For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477 source Swiss-Prot : SWS_FT_FI1 44) chain B residue 840 type ACT_SITE sequence A description Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477 source Swiss-Prot : SWS_FT_FI2 45) chain B residue 983 type BINDING sequence H description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 46) chain B residue 1297 type BINDING sequence H description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 47) chain B residue 1328 type BINDING sequence D description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 48) chain B residue 10 type BINDING sequence A description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3 49) chain B residue 762 type BINDING sequence E description BINDING => ECO:0000269|PubMed:24505130 source Swiss-Prot : SWS_FT_FI3

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