eF-site ID 5b2s-ACD PDB Code 5b2s Chain A, C, D click to enlarge Title Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM) Classification HYDROLASE/RNA/DNA Compound Guide RNA Source null (5B2S) Sequence A:  GGAAAUUAGGUGCGCUUGGCGUUUUAGAGCUAGAAAUAGC AAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGU G C:  CAATCTCAGCCAAGCGCACCTAATTTCC D:  TGAGATTG Description Functional site 1) chain A residue 65 type sequence A description binding site for residue K A 101 source : AC1 2) chain A residue 66 type sequence U description binding site for residue K A 101 source : AC1 3) chain A residue 68 type sequence A description binding site for residue K A 102 source : AC2 4) chain A residue 21 type sequence G description binding site for residue K A 103 source : AC3 5) chain A residue 50 type sequence U description binding site for residue MG A 104 source : AC4 6) chain A residue 63 type sequence U description binding site for residue EDO A 106 source : AC6 7) chain A residue 64 type sequence U description binding site for residue EDO A 106 source : AC6 8) chain A residue 66 type sequence U description binding site for residue ACT A 107 source : AC7 9) chain A residue 67 type sequence C description binding site for residue ACT A 107 source : AC7 10) chain A residue 22 type sequence U description binding site for residue K B 1405 source : AD3 11) chain A residue 52 type sequence A description binding site for residue EDO B 1410 source : AD8 12) chain A residue 19 type sequence G description binding site for residue EDO B 1412 source : AE1 13) chain A residue 20 type sequence C description binding site for residue EDO B 1412 source : AE1

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