eF-site ID 5a77-ABCDEF PDB Code 5a77 Chain A, B, C, D, E, F click to enlarge Title Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA cleaved Classification HYDROLASE/DNA Compound DNA ENDONUCLEASE I-CVUI Source Chlorella vulgaris (Green alga) (5A77) Sequence A:  NFHDQLKFAWLAGFVDADGCINAQIVSREDYLLKYQVRVS LTVFQSTTQHFILLDIQKILGCGTVRKRNDGMSEFCVVGG TSLQTTLEKLLPYLQLKRAQAKLVLQIIKKLPNTKDPSVL MEAALLADKVGLLTDGKKRTILAENVRECLKKLGHVV B:  NFHDQLKFAWLAGFVDADGCINAQIVSREDYLLKYQVRVS LTVFQSTTQHFILLDIQKILGCGTVRKRNDGMSEFCVVGG TSLQTTLEKLLPYLQLKRAQAKLVLQIIKKLPNTKDPSVL MEAALLADKVGLLTDGKKRTILAENVRECLKKLGHVVS C:  TCAAAACGTCGTAC D:  TCAAAACGTCGTAC E:  GACGTTTTGA F:  GACGTTTTGA Description Functional site 1) chain A residue 22 type sequence A description BINDING SITE FOR RESIDUE MG D 1615 source : AC1 2) chain B residue 23 type sequence D description BINDING SITE FOR RESIDUE MG D 1615 source : AC1 3) chain D residue 614 type sequence C description BINDING SITE FOR RESIDUE MG D 1615 source : AC1 4) chain E residue 515 type sequence G description BINDING SITE FOR RESIDUE MG D 1615 source : AC1 5) chain A residue 23 type sequence D description BINDING SITE FOR RESIDUE MG B 1164 source : AC2 6) chain B residue 22 type sequence A description BINDING SITE FOR RESIDUE MG B 1164 source : AC2 7) chain B residue 23 type sequence D description BINDING SITE FOR RESIDUE MG B 1164 source : AC2 8) chain C residue 514 type sequence C description BINDING SITE FOR RESIDUE MG B 1164 source : AC2 9) chain F residue 615 type sequence G description BINDING SITE FOR RESIDUE MG B 1164 source : AC2

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