eF-site ID 5a74-ABCDEF PDB Code 5a74 Chain A, B, C, D, E, F click to enlarge Title Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Mn Classification HYDROLASE/DNA Compound DNA ENDONUCLEASE I-CVUI Source Chlorella vulgaris (Green alga) (5A74) Sequence A:  NFHDQLKFAWLAGFVDADGCINAQIVSREDYLLKYQVRVS LTVFQSTTQHFILLDIQKILGCGTVRKRNDGMSEFCVVGG TSLQTTLEKLLPYLQLKRAQAKLVLQIIKKLPNTKDPSVL MEAALLADKVGLLTDGKKRTILAENVRECLKKLGHVV B:  NFHDQLKFAWLAGFVDADGCINAQIVSREDYLLKYQVRVS LTVFQSTTQHFILLDIQKILGCGTVRKRNDGMSEFCVVGG TSLQTTLEKLLPYLQLKRAQAKLVLQIIKKLPNTKDPSVL MEAALLADKVGLLTDGKKRTILAENVRECLKKLGHVVS C:  TCAGAACGTCGTAC D:  TCAGAACGTCGTAC E:  GACGTTCTGA F:  GACGTTCTGA Description Functional site 1) chain A residue 23 type sequence D description BINDING SITE FOR RESIDUE MN F 1625 source : AC1 2) chain B residue 22 type sequence A description BINDING SITE FOR RESIDUE MN F 1625 source : AC1 3) chain C residue 514 type sequence C description BINDING SITE FOR RESIDUE MN F 1625 source : AC1 4) chain F residue 615 type sequence G description BINDING SITE FOR RESIDUE MN F 1625 source : AC1 5) chain A residue 23 type sequence D description BINDING SITE FOR RESIDUE MN F 1626 source : AC2 6) chain B residue 23 type sequence D description BINDING SITE FOR RESIDUE MN F 1626 source : AC2 7) chain C residue 514 type sequence C description BINDING SITE FOR RESIDUE MN F 1626 source : AC2 8) chain D residue 614 type sequence C description BINDING SITE FOR RESIDUE MN F 1626 source : AC2 9) chain E residue 515 type sequence G description BINDING SITE FOR RESIDUE MN F 1626 source : AC2 10) chain F residue 615 type sequence G description BINDING SITE FOR RESIDUE MN F 1626 source : AC2 11) chain A residue 22 type sequence A description BINDING SITE FOR RESIDUE MN E 1525 source : AC3 12) chain B residue 23 type sequence D description BINDING SITE FOR RESIDUE MN E 1525 source : AC3 13) chain D residue 614 type sequence C description BINDING SITE FOR RESIDUE MN E 1525 source : AC3 14) chain E residue 515 type sequence G description BINDING SITE FOR RESIDUE MN E 1525 source : AC3

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