eF-site/Summary 4zup-AB

eF-site ID	4zup-AB
PDB Code	4zup
Chain	A, B

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Title	Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a hydroxamate inhibitor
Classification	Hydrolase/Hydrolase inhibitor
Compound	Acetylpolyamine aminohydrolase
Source	(APHA_MYCRA)

Sequence	A:	MRVIFSEDHKLRNAKTELYGGELVPPFEAPFRAEWILAAV KEAGFDDVVAPARHGLETVLKVHDAGYLNFLETAWDRWKA AGYKGEAIATSFPVRRTSPRIPTDIEGQIGYYCNAAETAI SPGTWEAALSSMASAIDGADLIAAGHKAAFSLCRPPGHHA GIDMFGGYCFINNAAVAAQRLLDKGAKKIAILDVDFHHGN GTQDIFYERGDVFFASLHGDPAEAFPHFLGYAEETGKGAG AGTTANYPMGRGTPYSVWGEALTDSLKRIAAFGAEAIVVS LGVDTFEQDPISFFKLTSPDYITMGRTIAASGVPLLVVME GGYGVPEIGLNVANVLKGVAG
	B:	MRVIFSEDHKLRNAKTELYGGELVPPFEAPFRAEWILAAV KEAGFDDVVAPARHGLETVLKVHDAGYLNFLETAWDRWKA AGYKGEAIATSFPVRRTSPRIPTDIEGQIGYYCNAAETAI SPGTWEAALSSMASAIDGADLIAAGHKAAFSLCRPPGHHA GIDMFGGYCFINNAAVAAQRLLDKGAKKIAILDVDFHHGN GTQDIFYERGDVFFASLHGDPAEAFPHFLGYAEETGKGAG AGTTANYPMGRGTPYSVWGEALTDSLKRIAAFGAEAIVVS LGVDTFEQDPISFFKLTSPDYITMGRTIAASGVPLLVVME GGYGVPEIGLNVANVLKGVAG

Description

Functional site


1)	chain	A
	residue	195
	type
	sequence	D
	description	binding site for residue ZN A 401
	source	: AC1

2)	chain	A
	residue	197
	type
	sequence	H
	description	binding site for residue ZN A 401
	source	: AC1

3)	chain	A
	residue	284
	type
	sequence	D
	description	binding site for residue ZN A 401
	source	: AC1

4)	chain	A
	residue	146
	type
	sequence	H
	description	binding site for residue ZN A 402
	source	: AC2

5)	chain	A
	residue	193
	type
	sequence	D
	description	binding site for residue K A 403
	source	: AC3

6)	chain	A
	residue	195
	type
	sequence	D
	description	binding site for residue K A 403
	source	: AC3

7)	chain	A
	residue	197
	type
	sequence	H
	description	binding site for residue K A 403
	source	: AC3

8)	chain	A
	residue	216
	type
	sequence	S
	description	binding site for residue K A 403
	source	: AC3

9)	chain	A
	residue	217
	type
	sequence	L
	description	binding site for residue K A 403
	source	: AC3

10)	chain	A
	residue	206
	type
	sequence	F
	description	binding site for residue K A 404
	source	: AC4

11)	chain	A
	residue	209
	type
	sequence	R
	description	binding site for residue K A 404
	source	: AC4

12)	chain	A
	residue	212
	type
	sequence	V
	description	binding site for residue K A 404
	source	: AC4

13)	chain	A
	residue	243
	type
	sequence	T
	description	binding site for residue K A 404
	source	: AC4

14)	chain	A
	residue	117
	type
	sequence	E
	description	binding site for residue 5XA A 405
	source	: AC5

15)	chain	A
	residue	158
	type
	sequence	H
	description	binding site for residue 5XA A 405
	source	: AC5

16)	chain	A
	residue	159
	type
	sequence	H
	description	binding site for residue 5XA A 405
	source	: AC5

17)	chain	A
	residue	168
	type
	sequence	Y
	description	binding site for residue 5XA A 405
	source	: AC5

18)	chain	A
	residue	195
	type
	sequence	D
	description	binding site for residue 5XA A 405
	source	: AC5

19)	chain	A
	residue	197
	type
	sequence	H
	description	binding site for residue 5XA A 405
	source	: AC5

20)	chain	A
	residue	225
	type
	sequence	F
	description	binding site for residue 5XA A 405
	source	: AC5

21)	chain	A
	residue	284
	type
	sequence	D
	description	binding site for residue 5XA A 405
	source	: AC5

22)	chain	A
	residue	323
	type
	sequence	Y
	description	binding site for residue 5XA A 405
	source	: AC5

23)	chain	A
	residue	15
	type
	sequence	K
	description	binding site for residue GOL A 406
	source	: AC6

24)	chain	A
	residue	75
	type
	sequence	W
	description	binding site for residue GOL A 406
	source	: AC6

25)	chain	A
	residue	84
	type
	sequence	K
	description	binding site for residue GOL A 406
	source	: AC6

26)	chain	A
	residue	85
	type
	sequence	G
	description	binding site for residue GOL A 406
	source	: AC6

27)	chain	A
	residue	1
	type
	sequence	M
	description	binding site for residue NO3 A 407
	source	: AC7

28)	chain	A
	residue	45
	type
	sequence	F
	description	binding site for residue NO3 A 407
	source	: AC7

29)	chain	A
	residue	340
	type
	sequence	A
	description	binding site for residue NO3 A 407
	source	: AC7

30)	chain	A
	residue	1
	type
	sequence	M
	description	binding site for residue NO3 A 408
	source	: AC8

31)	chain	A
	residue	146
	type
	sequence	H
	description	binding site for residue NO3 A 408
	source	: AC8

32)	chain	A
	residue	147
	type
	sequence	K
	description	binding site for residue NO3 A 408
	source	: AC8

33)	chain	A
	residue	148
	type
	sequence	A
	description	binding site for residue NO3 A 408
	source	: AC8

34)	chain	A
	residue	231
	type
	sequence	Y
	description	binding site for residue GOL A 409
	source	: AC9

35)	chain	A
	residue	232
	type
	sequence	A
	description	binding site for residue GOL A 409
	source	: AC9

36)	chain	A
	residue	233
	type
	sequence	E
	description	binding site for residue GOL A 409
	source	: AC9

37)	chain	B
	residue	100
	type
	sequence	R
	description	binding site for residue GOL A 409
	source	: AC9

38)	chain	B
	residue	195
	type
	sequence	D
	description	binding site for residue ZN B 401
	source	: AD1

39)	chain	B
	residue	197
	type
	sequence	H
	description	binding site for residue ZN B 401
	source	: AD1

40)	chain	B
	residue	284
	type
	sequence	D
	description	binding site for residue ZN B 401
	source	: AD1

41)	chain	B
	residue	146
	type
	sequence	H
	description	binding site for residue ZN B 402
	source	: AD2

42)	chain	B
	residue	193
	type
	sequence	D
	description	binding site for residue K B 403
	source	: AD3

43)	chain	B
	residue	195
	type
	sequence	D
	description	binding site for residue K B 403
	source	: AD3

44)	chain	B
	residue	197
	type
	sequence	H
	description	binding site for residue K B 403
	source	: AD3

45)	chain	B
	residue	216
	type
	sequence	S
	description	binding site for residue K B 403
	source	: AD3

46)	chain	B
	residue	217
	type
	sequence	L
	description	binding site for residue K B 403
	source	: AD3

47)	chain	B
	residue	206
	type
	sequence	F
	description	binding site for residue K B 404
	source	: AD4

48)	chain	B
	residue	209
	type
	sequence	R
	description	binding site for residue K B 404
	source	: AD4

49)	chain	B
	residue	212
	type
	sequence	V
	description	binding site for residue K B 404
	source	: AD4

50)	chain	B
	residue	243
	type
	sequence	T
	description	binding site for residue K B 404
	source	: AD4

51)	chain	B
	residue	117
	type
	sequence	E
	description	binding site for residue 5XA B 405
	source	: AD5

52)	chain	B
	residue	158
	type
	sequence	H
	description	binding site for residue 5XA B 405
	source	: AD5

53)	chain	B
	residue	159
	type
	sequence	H
	description	binding site for residue 5XA B 405
	source	: AD5

54)	chain	B
	residue	167
	type
	sequence	G
	description	binding site for residue 5XA B 405
	source	: AD5

55)	chain	B
	residue	168
	type
	sequence	Y
	description	binding site for residue 5XA B 405
	source	: AD5

56)	chain	B
	residue	195
	type
	sequence	D
	description	binding site for residue 5XA B 405
	source	: AD5

57)	chain	B
	residue	197
	type
	sequence	H
	description	binding site for residue 5XA B 405
	source	: AD5

58)	chain	B
	residue	225
	type
	sequence	F
	description	binding site for residue 5XA B 405
	source	: AD5

59)	chain	B
	residue	284
	type
	sequence	D
	description	binding site for residue 5XA B 405
	source	: AD5

60)	chain	B
	residue	323
	type
	sequence	Y
	description	binding site for residue 5XA B 405
	source	: AD5

61)	chain	B
	residue	96
	type
	sequence	R
	description	binding site for residue GOL B 406
	source	: AD6

62)	chain	B
	residue	1
	type
	sequence	M
	description	binding site for residue NO3 B 407
	source	: AD7

63)	chain	B
	residue	45
	type
	sequence	F
	description	binding site for residue NO3 B 407
	source	: AD7

64)	chain	B
	residue	340
	type
	sequence	A
	description	binding site for residue NO3 B 407
	source	: AD7

65)	chain	B
	residue	1
	type
	sequence	M
	description	binding site for residue NO3 B 408
	source	: AD8

66)	chain	B
	residue	146
	type
	sequence	H
	description	binding site for residue NO3 B 408
	source	: AD8

67)	chain	B
	residue	147
	type
	sequence	K
	description	binding site for residue NO3 B 408
	source	: AD8

68)	chain	B
	residue	148
	type
	sequence	A
	description	binding site for residue NO3 B 408
	source	: AD8

69)	chain	B
	residue	266
	type
	sequence	L
	description	binding site for residue GOL B 409
	source	: AD9

70)	chain	B
	residue	307
	type
	sequence	T
	description	binding site for residue GOL B 409
	source	: AD9

71)	chain	B
	residue	310
	type
	sequence	A
	description	binding site for residue GOL B 409
	source	: AD9

72)	chain	B
	residue	311
	type
	sequence	S
	description	binding site for residue GOL B 409
	source	: AD9

73)	chain	A
	residue	19
	type	BINDING
	sequence	Y
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI2

74)	chain	A
	residue	117
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI2

75)	chain	A
	residue	323
	type	BINDING
	sequence	Y
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI2

76)	chain	B
	residue	19
	type	BINDING
	sequence	Y
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI2

77)	chain	B
	residue	117
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI2

78)	chain	B
	residue	323
	type	BINDING
	sequence	Y
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI2

79)	chain	A
	residue	159
	type	ACT_SITE
	sequence	H
	description	Proton donor/acceptor => ECO:0000305\|PubMed:21268586, ECO:0000305\|PubMed:26200446
	source	Swiss-Prot : SWS_FT_FI1

80)	chain	B
	residue	159
	type	ACT_SITE
	sequence	H
	description	Proton donor/acceptor => ECO:0000305\|PubMed:21268586, ECO:0000305\|PubMed:26200446
	source	Swiss-Prot : SWS_FT_FI1

81)	chain	A
	residue	106
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9C
	source	Swiss-Prot : SWS_FT_FI3

82)	chain	B
	residue	106
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0007744\|PDB:3Q9C
	source	Swiss-Prot : SWS_FT_FI3

83)	chain	A
	residue	195
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0000269\|PubMed:26200446, ECO:0007744\|PDB:3Q9B, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI4

84)	chain	A
	residue	197
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0000269\|PubMed:26200446, ECO:0007744\|PDB:3Q9B, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI4

85)	chain	A
	residue	284
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0000269\|PubMed:26200446, ECO:0007744\|PDB:3Q9B, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI4

86)	chain	B
	residue	195
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0000269\|PubMed:26200446, ECO:0007744\|PDB:3Q9B, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI4

87)	chain	B
	residue	197
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0000269\|PubMed:26200446, ECO:0007744\|PDB:3Q9B, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI4

88)	chain	B
	residue	284
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000269\|PubMed:21268586, ECO:0000269\|PubMed:26200446, ECO:0007744\|PDB:3Q9B, ECO:0007744\|PDB:3Q9E
	source	Swiss-Prot : SWS_FT_FI4

89)	chain	A
	residue	323
	type	SITE
	sequence	Y
	description	Polarizes the scissile carbonyl of the substrate => ECO:0000305\|PubMed:21268586
	source	Swiss-Prot : SWS_FT_FI5

90)	chain	B
	residue	323
	type	SITE
	sequence	Y
	description	Polarizes the scissile carbonyl of the substrate => ECO:0000305\|PubMed:21268586
	source	Swiss-Prot : SWS_FT_FI5