eF-site ID 4ylo-I PDB Code 4ylo Chain I click to enlarge Title E. coli Transcription Initiation Complex - 16-bp spacer and 4-nt RNA Classification TRANSCRIPTION/DNA/RNA Compound DNA-directed RNA polymerase subunit alpha Source (4YLO) Sequence I:  VYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQ DPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFD VQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQE VYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDK GKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRR RKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMEL VPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDD VKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDL LAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLS ALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVG RMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGE VDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSL GDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPL SEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPI ETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTD EIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGE SSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALM GANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRG GVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQ NTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMR VAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSR DTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGD ILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVP NGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLS EELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELG LTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDD LAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINP IEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAA KGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDL STFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKL GDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDD KMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAY GAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPE SFNVLLKEIRSLGINIELEDE Description (1)  E. coli RNA Polymerase alpha subunit, E. coli RNA Polymerase beta subunit, E. coli RNA Polymerase beta' subunit, E. coli RNA Polymerase omega subunit, E. coli RNA Polymerase sigma70 subunit/DNA Complex Functional site 1) chain I residue 529 type sequence R description binding site for nucleotide GTP 6 13 source : AD2 2) chain I residue 564 type sequence P description binding site for nucleotide GTP 6 13 source : AD2 3) chain I residue 568 type sequence N description binding site for nucleotide GTP 6 13 source : AD2 4) chain I residue 688 type sequence Q description binding site for nucleotide GTP 6 13 source : AD2 5) chain I residue 1065 type sequence K description binding site for nucleotide GTP 6 13 source : AD2

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