eF-site ID 4w6u-ABCD PDB Code 4w6u Chain A, B, C, D click to enlarge Title Crystal Structure of Full-Length Split GFP Mutant E115H/T118H With Nickel Mediated Crystal Contacts, P 21 21 21 Space Group Classification FLUORESCENT PROTEIN Compound fluorescent protein E115H/T118H Source (4W6U) Sequence A:  KGEELFTGVVPILIELDGDVNGHKFFVRGEGEGDATIGKL SLKFIATTGKLPVPWPTLVTTLXVQAFSRYPDHMKRHDFF KSAMPEGYVQERTIYFKDDGTYKTRAEVKFHGDHLVNRIE LKGIDFKEDGNILGHKLEYNFNSHKVYITADKQNNGIKAN FTIRHNVEDGSVQLADHYQQNTPIGDVDLPDDHYLSTQTI LSKDLNEKRDHMVLLEYVTAAGI B:  GEELFTGVVPILIELDGDVNGHKFFVRGEGEGDATIGKLS LKFIATTGKLPVPWPTLVTTLXVQAFSRYPDHMKRHDFFK SAMPEGYVQERTIYFKDDGTYKTRAEVKFHGDHLVNRIEL KGIDFKEDGNILGHKLEYNFNSHKVYITADKQNNGIKANF TIRHNVEDGSVQLADHYQQNTPIGDGPVDLPDDHYLSTQT ILSKDLNEKRDHMVLLEYVTAA C:  KGEELFTGVVPILIELDGDVNGHKFFVRGEGEGDATIGKL SLKFIATTGKLPVPWPTLVTTLXVQAFSRYPDHMKRHDFF KSAMPEGYVQERTIYFKDDGTYKTRAEVKFHGDHLVNRIE LKGIDFKEDGNILGHKLEYNFNSHKVYITADKQNNGIKAN FTIRHNVEDGSVQLADHYQQNTPIGPVDLPDDHYLSTQTI LSKDLNEKRDHMVLLEYVTAAG D:  KGEELFTGVVPILIELDGDVNGHKFFVRGEGEGDATIGKL SLKFIATTGKLPVPWPTLVTTLXVQAFSRYPDHMKRHDFF KSAMPEGYVQERTIYFKDDGTYKTRAEVKFHGDHLVNRIE LKGIDFKEDGNILGHKLEYNFNSHKVYITADKQNNGIKAN FTIRHNVEDGSVQLADHYQQNTPIGDGVDLPDDHYLSTQT ILSKDLNEKRDHMVLLEYVTAAG Description Functional site 1) chain A residue 25 type sequence H description binding site for residue NI A 301 source : AC1 2) chain A residue 182 type sequence Y description binding site for residue EDO A 302 source : AC2 3) chain A residue 151 type sequence Y description binding site for residue EDO A 303 source : AC3 4) chain A residue 153 type sequence T description binding site for residue EDO A 303 source : AC3 5) chain A residue 164 type sequence N description binding site for residue EDO A 303 source : AC3 6) chain B residue 102 type sequence D description binding site for residue EDO A 303 source : AC3 7) chain A residue 118 type sequence H description binding site for residue NI B 301 source : AC4 8) chain B residue 115 type sequence H description binding site for residue NI B 301 source : AC4 9) chain C residue 32 type sequence E description binding site for residue CIT C 301 source : AC5 10) chain C residue 33 type sequence G description binding site for residue CIT C 301 source : AC5 11) chain C residue 118 type sequence H description binding site for residue CIT C 301 source : AC5

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