eF-site ID 4pij-AB PDB Code 4pij Chain A, B click to enlarge Title X-ray crystal structure of the K11S/K63S double mutant of ubiquitin Classification PROTEIN BINDING Compound Ubiquitin Source Homo sapiens (Human) (RL40_HUMAN) Sequence A:  MQIFVKTLTGSTITLEVEPSDTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYNIQSESTLHLVLRLRG B:  MQIFVKTLTGSTITLEVEPSDTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYNIQSESTLHLVLRLRG Description Functional site 1) chain A residue 7 type sequence T description binding site for residue GOL A 101 source : AC1 2) chain A residue 68 type sequence H description binding site for residue GOL A 101 source : AC1 3) chain A residue 69 type sequence L description binding site for residue GOL A 101 source : AC1 4) chain B residue 47 type sequence G description binding site for residue GOL A 101 source : AC1 5) chain B residue 27 type sequence K description binding site for residue SO4 B 101 source : AC2 6) chain B residue 39 type sequence D description binding site for residue SO4 B 101 source : AC2 7) chain B residue 42 type sequence R description binding site for residue SO4 B 101 source : AC2 8) chain B residue 52 type sequence D description binding site for residue SO4 B 101 source : AC2 9) chain B residue 54 type sequence R description binding site for residue SO4 B 101 source : AC2 10) chain A residue 2 type sequence Q description binding site for residue SO4 B 102 source : AC3 11) chain B residue 46 type sequence A description binding site for residue SO4 B 102 source : AC3 12) chain B residue 68 type sequence H description binding site for residue SO4 B 102 source : AC3 13) chain A residue 74 type sequence R description binding site for residue SO4 B 103 source : AC4 14) chain B residue 51 type sequence E description binding site for residue SO4 B 103 source : AC4 15) chain B residue 52 type sequence D description binding site for residue SO4 B 103 source : AC4 16) chain A residue 42 type sequence R description binding site for residue SO4 B 104 source : AC5 17) chain A residue 49 type sequence Q description binding site for residue SO4 B 104 source : AC5 18) chain A residue 72 type sequence R description binding site for residue SO4 B 104 source : AC5 19) chain B residue 42 type sequence R description binding site for residue SO4 B 104 source : AC5 20) chain B residue 49 type sequence Q description binding site for residue SO4 B 104 source : AC5 21) chain B residue 72 type sequence R description binding site for residue SO4 B 104 source : AC5 22) chain B residue 73 type sequence L description binding site for residue SO4 B 104 source : AC5 23) chain A residue 7 type sequence T description binding site for residue SO4 B 105 source : AC6 24) chain A residue 9 type sequence T description binding site for residue SO4 B 105 source : AC6 25) chain A residue 11 type sequence S description binding site for residue SO4 B 105 source : AC6 26) chain A residue 34 type sequence E description binding site for residue SO4 B 105 source : AC6 27) chain B residue 7 type sequence T description binding site for residue SO4 B 105 source : AC6 28) chain B residue 8 type sequence L description binding site for residue SO4 B 105 source : AC6 29) chain B residue 9 type sequence T description binding site for residue SO4 B 105 source : AC6 30) chain B residue 34 type sequence E description binding site for residue SO4 B 105 source : AC6 31) chain A residue 27-52 type prosite sequence KAKIQDKEGIPPDQQRLIFAGKQLED description UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD source prosite : PS00299 32) chain A residue 54 type SITE sequence R description Interacts with activating enzyme source Swiss-Prot : SWS_FT_FI1 33) chain A residue 72 type SITE sequence R description Interacts with activating enzyme source Swiss-Prot : SWS_FT_FI1 34) chain B residue 54 type SITE sequence R description Interacts with activating enzyme source Swiss-Prot : SWS_FT_FI1 35) chain B residue 72 type SITE sequence R description Interacts with activating enzyme source Swiss-Prot : SWS_FT_FI1 36) chain A residue 33 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577 source Swiss-Prot : SWS_FT_FI10 37) chain B residue 33 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577 source Swiss-Prot : SWS_FT_FI10 38) chain A residue 68 type SITE sequence H description Essential for function source Swiss-Prot : SWS_FT_FI2 39) chain B residue 68 type SITE sequence H description Essential for function source Swiss-Prot : SWS_FT_FI2 40) chain A residue 65 type MOD_RES sequence S description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25474007, ECO:0000269|PubMed:25527291 source Swiss-Prot : SWS_FT_FI3 41) chain B residue 65 type MOD_RES sequence S description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25474007, ECO:0000269|PubMed:25527291 source Swiss-Prot : SWS_FT_FI3 42) chain A residue 66 type MOD_RES sequence T description (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457 source Swiss-Prot : SWS_FT_FI4 43) chain B residue 66 type MOD_RES sequence T description (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457 source Swiss-Prot : SWS_FT_FI4 44) chain A residue 6 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 source Swiss-Prot : SWS_FT_FI5 45) chain B residue 6 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 source Swiss-Prot : SWS_FT_FI5 46) chain A residue 63 type CROSSLNK sequence S description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106 source Swiss-Prot : SWS_FT_FI6 47) chain B residue 63 type CROSSLNK sequence S description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106 source Swiss-Prot : SWS_FT_FI6 48) chain A residue 11 type CROSSLNK sequence S description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 source Swiss-Prot : SWS_FT_FI7 49) chain A residue 48 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 source Swiss-Prot : SWS_FT_FI7 50) chain B residue 11 type CROSSLNK sequence S description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 source Swiss-Prot : SWS_FT_FI7 51) chain B residue 48 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 source Swiss-Prot : SWS_FT_FI7 52) chain A residue 27 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860 source Swiss-Prot : SWS_FT_FI8 53) chain B residue 27 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860 source Swiss-Prot : SWS_FT_FI8 54) chain A residue 29 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127 source Swiss-Prot : SWS_FT_FI9 55) chain B residue 29 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127 source Swiss-Prot : SWS_FT_FI9

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