eF-site ID 4j89-AB
PDB Code 4j89
Chain A, B

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Title Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence
Classification FLUORESCENT PROTEIN
Compound Green fluorescent protein
Source Aequorea victoria (Jellyfish) (GFP_AEQVI)
Sequence A:  VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNG
KLTLKFICTTGKLPVPWPTLVTTLXVQCFSRYPDHMKRHD
FFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNR
IELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLS
TQSVLSKDPNEKRDHMVLLEFVTAAGITH
B:  SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGK
LTLKFICTTGKLPVPWPTLVTTLXVQCFSRYPDHMKRHDF
FKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRI
ELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST
QSVLSKDPNEKRDHMVLLEFVTAAGIT
Description


Functional site

1) chain A
residue 80
type
sequence R
description BINDING SITE FOR RESIDUE SO4 A 301
source : AC1

2) chain A
residue 116
type
sequence G
description BINDING SITE FOR RESIDUE SO4 A 301
source : AC1

3) chain A
residue 117
type
sequence D
description BINDING SITE FOR RESIDUE SO4 A 301
source : AC1

4) chain A
residue 118
type
sequence T
description BINDING SITE FOR RESIDUE SO4 A 301
source : AC1

5) chain A
residue 55
type
sequence V
description BINDING SITE FOR RESIDUE SO4 A 302
source : AC2

6) chain A
residue 56
type
sequence P
description BINDING SITE FOR RESIDUE SO4 A 302
source : AC2

7) chain A
residue 57
type
sequence W
description BINDING SITE FOR RESIDUE SO4 A 302
source : AC2

8) chain A
residue 58
type
sequence P
description BINDING SITE FOR RESIDUE SO4 A 302
source : AC2

9) chain A
residue 139
type
sequence H
description BINDING SITE FOR RESIDUE SO4 A 302
source : AC2

10) chain A
residue 73
type
sequence R
description BINDING SITE FOR RESIDUE TRS A 303
source : AC3

11) chain A
residue 75
type
sequence P
description BINDING SITE FOR RESIDUE TRS A 303
source : AC3

12) chain A
residue 76
type
sequence D
description BINDING SITE FOR RESIDUE TRS A 303
source : AC3

13) chain A
residue 77
type
sequence H
description BINDING SITE FOR RESIDUE TRS A 303
source : AC3

14) chain B
residue 149
type
sequence N
description BINDING SITE FOR RESIDUE TRS A 303
source : AC3

15) chain B
residue 151
type
sequence Y
description BINDING SITE FOR RESIDUE TRS A 303
source : AC3

16) chain A
residue 95
type
sequence E
description BINDING SITE FOR RESIDUE EDO A 304
source : AC4

17) chain A
residue 109
type
sequence R
description BINDING SITE FOR RESIDUE EDO A 304
source : AC4

18) chain B
residue 3
type
sequence K
description BINDING SITE FOR RESIDUE EDO A 304
source : AC4

19) chain A
residue 124
type
sequence E
description BINDING SITE FOR RESIDUE EDO A 305
source : AC5

20) chain B
residue 194
type
sequence L
description BINDING SITE FOR RESIDUE EDO A 305
source : AC5

21) chain A
residue 109
type
sequence R
description BINDING SITE FOR RESIDUE EDO A 306
source : AC6

22) chain A
residue 124
type
sequence E
description BINDING SITE FOR RESIDUE EDO A 306
source : AC6

23) chain A
residue 30
type
sequence R
description BINDING SITE FOR RESIDUE EDO A 307
source : AC7

24) chain A
residue 143
type
sequence Y
description BINDING SITE FOR RESIDUE EDO A 308
source : AC8

25) chain A
residue 208
type
sequence S
description BINDING SITE FOR RESIDUE EDO A 308
source : AC8

26) chain A
residue 209
type
sequence K
description BINDING SITE FOR RESIDUE EDO A 308
source : AC8

27) chain A
residue 143
type
sequence Y
description BINDING SITE FOR RESIDUE EDO A 309
source : AC9

28) chain A
residue 144
type
sequence N
description BINDING SITE FOR RESIDUE EDO A 309
source : AC9

29) chain A
residue 205
type
sequence S
description BINDING SITE FOR RESIDUE EDO A 309
source : AC9

30) chain A
residue 206
type
sequence V
description BINDING SITE FOR RESIDUE EDO A 309
source : AC9

31) chain A
residue 207
type
sequence L
description BINDING SITE FOR RESIDUE EDO A 309
source : AC9

32) chain A
residue 90
type
sequence E
description BINDING SITE FOR RESIDUE EDO A 310
source : BC1

33) chain A
residue 113
type
sequence K
description BINDING SITE FOR RESIDUE EDO A 310
source : BC1

34) chain A
residue 188
type
sequence I
description BINDING SITE FOR RESIDUE EDO A 310
source : BC1

35) chain A
residue 132
type
sequence E
description BINDING SITE FOR RESIDUE EDO A 311
source : BC2

36) chain A
residue 149
type
sequence N
description BINDING SITE FOR RESIDUE EDO A 312
source : BC3

37) chain A
residue 151
type
sequence Y
description BINDING SITE FOR RESIDUE EDO A 312
source : BC3

38) chain B
residue 75
type
sequence P
description BINDING SITE FOR RESIDUE EDO A 312
source : BC3

39) chain B
residue 76
type
sequence D
description BINDING SITE FOR RESIDUE EDO A 312
source : BC3

40) chain A
residue 102
type
sequence D
description BINDING SITE FOR RESIDUE EDO A 313
source : BC4

41) chain A
residue 103
type
sequence D
description BINDING SITE FOR RESIDUE EDO A 313
source : BC4

42) chain A
residue 144
type
sequence N
description BINDING SITE FOR RESIDUE EDO A 314
source : BC5

43) chain A
residue 190
type
sequence D
description BINDING SITE FOR RESIDUE EDO A 315
source : BC6

44) chain B
residue 56
type
sequence P
description BINDING SITE FOR RESIDUE EDO A 315
source : BC6

45) chain B
residue 57
type
sequence W
description BINDING SITE FOR RESIDUE EDO A 315
source : BC6

46) chain B
residue 58
type
sequence P
description BINDING SITE FOR RESIDUE EDO A 315
source : BC6

47) chain B
residue 139
type
sequence H
description BINDING SITE FOR RESIDUE EDO A 315
source : BC6

48) chain A
residue 148
type
sequence H
description BINDING SITE FOR RESIDUE EDO A 316
source : BC7

49) chain A
residue 165
type
sequence F
description BINDING SITE FOR RESIDUE EDO A 316
source : BC7

50) chain A
residue 166
type
sequence K
description BINDING SITE FOR RESIDUE EDO A 316
source : BC7

51) chain A
residue 168
type
sequence R
description BINDING SITE FOR RESIDUE EDO A 316
source : BC7

52) chain B
residue 101
type
sequence K
description BINDING SITE FOR RESIDUE SO4 B 301
source : BC8

53) chain B
residue 102
type
sequence D
description BINDING SITE FOR RESIDUE SO4 B 301
source : BC8

54) chain B
residue 103
type
sequence D
description BINDING SITE FOR RESIDUE SO4 B 301
source : BC8

55) chain B
residue 148
type
sequence H
description BINDING SITE FOR RESIDUE SO4 B 302
source : BC9

56) chain B
residue 165
type
sequence F
description BINDING SITE FOR RESIDUE SO4 B 302
source : BC9

57) chain B
residue 166
type
sequence K
description BINDING SITE FOR RESIDUE SO4 B 302
source : BC9

58) chain A
residue 41
type
sequence K
description BINDING SITE FOR RESIDUE SO4 B 303
source : CC1

59) chain B
residue 144
type
sequence N
description BINDING SITE FOR RESIDUE SO4 B 303
source : CC1

60) chain B
residue 145
type
sequence F
description BINDING SITE FOR RESIDUE SO4 B 303
source : CC1

61) chain B
residue 205
type
sequence S
description BINDING SITE FOR RESIDUE SO4 B 303
source : CC1

62) chain B
residue 206
type
sequence V
description BINDING SITE FOR RESIDUE SO4 B 303
source : CC1

63) chain B
residue 207
type
sequence L
description BINDING SITE FOR RESIDUE SO4 B 303
source : CC1

64) chain A
residue 107
type
sequence K
description BINDING SITE FOR RESIDUE EDO B 304
source : CC2

65) chain A
residue 126
type
sequence K
description BINDING SITE FOR RESIDUE EDO B 304
source : CC2

66) chain B
residue 194
type
sequence L
description BINDING SITE FOR RESIDUE EDO B 304
source : CC2

67) chain B
residue 195
type
sequence L
description BINDING SITE FOR RESIDUE EDO B 304
source : CC2

68) chain A
residue 89
type
sequence P
description BINDING SITE FOR RESIDUE EDO B 305
source : CC3

69) chain A
residue 90
type
sequence E
description BINDING SITE FOR RESIDUE EDO B 305
source : CC3

70) chain B
residue 143
type
sequence Y
description BINDING SITE FOR RESIDUE EDO B 305
source : CC3

71) chain B
residue 208
type
sequence S
description BINDING SITE FOR RESIDUE EDO B 305
source : CC3

72) chain B
residue 209
type
sequence K
description BINDING SITE FOR RESIDUE EDO B 305
source : CC3

73) chain B
residue 17
type
sequence E
description BINDING SITE FOR RESIDUE EDO B 306
source : CC4

74) chain B
residue 29
type
sequence V
description BINDING SITE FOR RESIDUE EDO B 306
source : CC4

75) chain B
residue 30
type
sequence R
description BINDING SITE FOR RESIDUE EDO B 306
source : CC4

76) chain B
residue 208
type
sequence S
description BINDING SITE FOR RESIDUE EDO B 307
source : CC5

77) chain B
residue 209
type
sequence K
description BINDING SITE FOR RESIDUE EDO B 307
source : CC5

78) chain A
residue 200
type
sequence Y
description BINDING SITE FOR RESIDUE EDO B 308
source : CC6

79) chain A
residue 228
type
sequence G
description BINDING SITE FOR RESIDUE EDO B 308
source : CC6

80) chain A
residue 229
type
sequence I
description BINDING SITE FOR RESIDUE EDO B 308
source : CC6

81) chain B
residue 77
type
sequence H
description BINDING SITE FOR RESIDUE EDO B 308
source : CC6

82) chain B
residue 227
type
sequence A
description BINDING SITE FOR RESIDUE EDO B 308
source : CC6

83) chain B
residue 228
type
sequence G
description BINDING SITE FOR RESIDUE EDO B 308
source : CC6

84) chain B
residue 229
type
sequence I
description BINDING SITE FOR RESIDUE EDO B 308
source : CC6

85) chain B
residue 164
type
sequence N
description BINDING SITE FOR RESIDUE EDO B 309
source : CC7

86) chain B
residue 34
type
sequence E
description BINDING SITE FOR RESIDUE EDO B 310
source : CC8

87) chain B
residue 36
type
sequence D
description BINDING SITE FOR RESIDUE EDO B 310
source : CC8

88) chain B
residue 41
type
sequence K
description BINDING SITE FOR RESIDUE EDO B 310
source : CC8

89) chain B
residue 42
type
sequence L
description BINDING SITE FOR RESIDUE EDO B 310
source : CC8

90) chain B
residue 43
type
sequence T
description BINDING SITE FOR RESIDUE EDO B 310
source : CC8

91) chain B
residue 151
type
sequence Y
description BINDING SITE FOR RESIDUE EDO B 311
source : CC9

92) chain B
residue 152
type
sequence I
description BINDING SITE FOR RESIDUE EDO B 311
source : CC9

93) chain B
residue 198
type
sequence N
description BINDING SITE FOR RESIDUE EDO B 311
source : CC9

94) chain B
residue 199
type
sequence H
description BINDING SITE FOR RESIDUE EDO B 311
source : CC9

95) chain B
residue 228
type
sequence G
description BINDING SITE FOR RESIDUE EDO B 311
source : CC9

96) chain B
residue 166
type
sequence K
description BINDING SITE FOR RESIDUE EDO B 312
source : DC1

97) chain B
residue 80
type
sequence R
description BINDING SITE FOR RESIDUE EDO B 313
source : DC2

98) chain B
residue 194
type
sequence L
description BINDING SITE FOR RESIDUE EDO B 313
source : DC2

99) chain B
residue 195
type
sequence L
description BINDING SITE FOR RESIDUE EDO B 313
source : DC2

100) chain A
residue 68
type MOD_RES
sequence V
description (Z)-2,3-didehydrotyrosine => ECO:0000269|PubMed:8448132
source Swiss-Prot : SWS_FT_FI1

101) chain B
residue 68
type MOD_RES
sequence V
description (Z)-2,3-didehydrotyrosine => ECO:0000269|PubMed:8448132
source Swiss-Prot : SWS_FT_FI1

102) chain A
residue 66
type CROSSLNK
sequence X
description 5-imidazolinone (Ser-Gly) => ECO:0000269|PubMed:8448132
source Swiss-Prot : SWS_FT_FI2

103) chain A
residue 69
type CROSSLNK
sequence Q
description 5-imidazolinone (Ser-Gly) => ECO:0000269|PubMed:8448132
source Swiss-Prot : SWS_FT_FI2

104) chain B
residue 66
type CROSSLNK
sequence X
description 5-imidazolinone (Ser-Gly) => ECO:0000269|PubMed:8448132
source Swiss-Prot : SWS_FT_FI2

105) chain B
residue 69
type CROSSLNK
sequence Q
description 5-imidazolinone (Ser-Gly) => ECO:0000269|PubMed:8448132
source Swiss-Prot : SWS_FT_FI2


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