eF-site ID 4iub-LS PDB Code 4iub Chain L, S click to enlarge Title Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 1 Classification OXIDOREDUCTASE Compound Uptake hydrogenase large subunit Source Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) (MBHS_CUPNH) Sequence L:  SAYATQGFNLDDRGRRIVVDPVTRIEGHMRCEVNVDANNV IRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGC HALASVRAVENALDIRIPKNAHLIREIMAKTLQVHDHAVH FYHLHALDWVDVMSALKADPKRTSELQQLVSPAHPLSSAG YFRDIQNRLKRFVESGQLGPFMNGYWGSKAYVLPPEANLM AVTHYLEALDLQKEWVKIHTIFGGKNPHPNYLVGGVPCAI NLDGIGAASAPVNMERLSFVKARIDEIIEFNKNVYVPDVL AIGTLYKQAGWLYGGGLAATNVLDYGEYPNVAYNKSTDQL PGGAILNGNWDEVFPVDPRDSQQVQEFVSHSWYKYADESV GLHPWDGVTEPNYVLGANTKGTRTRIEQIDESAKYSWIKS PRWRGHAMEVGPLSRYILAYAHARSGNKYAERPKEQLEYS AQMINSAIPKALGLPETQYTLKQLLPSTIGRTLARALESQ YCGEMMHSDWHDLVANIRAGDTATANVDKWDPATWPLQAK GVGTVAAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPRD YKGQIGAFEASLMNTPMVNPEQPVEILRTLHSFDPCLACS TH S:  PRTPVLWLHGLECTCCSESFIRSAHPLAKDVVLSMISLDY DDTLMAAAGHQAEAILEEIMTKYKGNYILAVEGNPPLNQD GMSCIIGGRPFIEQLKYVAKDAKAIISWGSCASWGCVQAA KPNPTQATPVHKVITDKPIIKVPGCPPIAEVMTGVITYML TFDRIPELDRQGRPKMFYSQRIHDKCYRRPHFDAGQFVEE WDDESARKGFCLYKMGCKGPTTYNACSTTRWNEGTSFPIQ SGHGCIGCSEDGFWDKGSFYDRLT Description Functional site 1) chain L residue 75 type sequence C description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 2) chain L residue 78 type sequence C description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 3) chain L residue 81 type sequence C description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 4) chain L residue 82 type sequence H description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 5) chain L residue 528 type sequence A description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 6) chain L residue 529 type sequence P description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 7) chain L residue 530 type sequence R description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 8) chain L residue 533 type sequence L description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 9) chain L residue 551 type sequence V description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 10) chain L residue 552 type sequence P description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 11) chain L residue 553 type sequence T description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 12) chain L residue 597 type sequence C description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 13) chain L residue 600 type sequence C description BINDING SITE FOR RESIDUE NFV L 701 source : AC1 14) chain L residue 56 type sequence E description BINDING SITE FOR RESIDUE MG L 702 source : AC2 15) chain L residue 549 type sequence C description BINDING SITE FOR RESIDUE MG L 702 source : AC2 16) chain L residue 603 type sequence H description BINDING SITE FOR RESIDUE MG L 702 source : AC2 17) chain L residue 88 type sequence R description BINDING SITE FOR RESIDUE PO4 L 703 source : AC3 18) chain L residue 98 type sequence I description BINDING SITE FOR RESIDUE PO4 L 703 source : AC3 19) chain L residue 103 type sequence H description BINDING SITE FOR RESIDUE PO4 L 703 source : AC3 20) chain L residue 106 type sequence R description BINDING SITE FOR RESIDUE PO4 L 703 source : AC3 21) chain L residue 521 type sequence K description BINDING SITE FOR RESIDUE PO4 L 704 source : AC4 22) chain L residue 538 type sequence R description BINDING SITE FOR RESIDUE PO4 L 704 source : AC4 23) chain S residue 168 type sequence R description BINDING SITE FOR RESIDUE PO4 L 704 source : AC4 24) chain S residue 169 type sequence I description BINDING SITE FOR RESIDUE PO4 L 704 source : AC4 25) chain L residue 501 type sequence G description BINDING SITE FOR RESIDUE PO4 L 705 source : AC5 26) chain L residue 502 type sequence D description BINDING SITE FOR RESIDUE PO4 L 705 source : AC5 27) chain L residue 503 type sequence T description BINDING SITE FOR RESIDUE PO4 L 705 source : AC5 28) chain L residue 504 type sequence A description BINDING SITE FOR RESIDUE PO4 L 705 source : AC5 29) chain S residue 211 type sequence R description BINDING SITE FOR RESIDUE PO4 L 705 source : AC5 30) chain L residue 247 type sequence G description BINDING SITE FOR RESIDUE PO4 L 706 source : AC6 31) chain L residue 248 type sequence A description BINDING SITE FOR RESIDUE PO4 L 706 source : AC6 32) chain L residue 249 type sequence A description BINDING SITE FOR RESIDUE PO4 L 706 source : AC6 33) chain L residue 383 type sequence T description BINDING SITE FOR RESIDUE PO4 L 707 source : AC7 34) chain L residue 384 type sequence R description BINDING SITE FOR RESIDUE PO4 L 707 source : AC7 35) chain S residue 187 type sequence H description BINDING SITE FOR RESIDUE SF4 S 1001 source : AC8 36) chain S residue 190 type sequence C description BINDING SITE FOR RESIDUE SF4 S 1001 source : AC8 37) chain S residue 193 type sequence R description BINDING SITE FOR RESIDUE SF4 S 1001 source : AC8 38) chain S residue 215 type sequence C description BINDING SITE FOR RESIDUE SF4 S 1001 source : AC8 39) chain S residue 216 type sequence L description BINDING SITE FOR RESIDUE SF4 S 1001 source : AC8 40) chain S residue 221 type sequence C description BINDING SITE FOR RESIDUE SF4 S 1001 source : AC8 41) chain L residue 226 type sequence K description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 42) chain S residue 226 type sequence T description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 43) chain S residue 228 type sequence N description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 44) chain S residue 230 type sequence C description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 45) chain S residue 235 type sequence W description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 46) chain S residue 242 type sequence P description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 47) chain S residue 249 type sequence C description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 48) chain S residue 250 type sequence I description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 49) chain S residue 252 type sequence C description BINDING SITE FOR RESIDUE F3S S 1002 source : AC9 50) chain L residue 73 type sequence R description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 51) chain L residue 229 type sequence H description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 52) chain S residue 17 type sequence C description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 53) chain S residue 18 type sequence T description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 54) chain S residue 19 type sequence C description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 55) chain S residue 20 type sequence C description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 56) chain S residue 76 type sequence E description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 57) chain S residue 114 type sequence S description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 58) chain S residue 115 type sequence C description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 59) chain S residue 120 type sequence C description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 60) chain S residue 149 type sequence C description BINDING SITE FOR RESIDUE S3F S 1003 source : BC1 61) chain L residue 342 type sequence S description BINDING SITE FOR RESIDUE PO4 S 1004 source : BC2 62) chain S residue 171 type sequence E description BINDING SITE FOR RESIDUE PO4 S 1004 source : BC2 63) chain S residue 172 type sequence L description BINDING SITE FOR RESIDUE PO4 S 1004 source : BC2 64) chain S residue 118 type sequence W description BINDING SITE FOR RESIDUE CL S 1005 source : BC3 65) chain S residue 120 type sequence C description BINDING SITE FOR RESIDUE CL S 1005 source : BC3 66) chain S residue 256 type sequence G description BINDING SITE FOR RESIDUE CL S 1005 source : BC3 67) chain L residue 53-78 type prosite sequence RGLEVILKGRDPRDAWAFVERICGVC description NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEvilkgrdprdawafvERiCGVC source prosite : PS00507 68) chain L residue 594-603 type prosite sequence FDPCLACSTH description NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H source prosite : PS00508 69) chain S residue 17 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 70) chain S residue 249 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 71) chain S residue 252 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 72) chain S residue 20 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 73) chain S residue 115 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 74) chain S residue 149 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 75) chain S residue 187 type BINDING sequence H description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 76) chain S residue 190 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 77) chain S residue 215 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 78) chain S residue 221 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1 79) chain S residue 230 type BINDING sequence C description BINDING => ECO:0000250|UniProtKB:P21853 source Swiss-Prot : SWS_FT_FI1

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