eF-site/Summary 4ca1-AB

eF-site ID	4ca1-AB
PDB Code	4ca1
Chain	A, B

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Title	Crystal structure of Siah1 at 1.58 A resolution.
Classification	LIGASE
Compound	E3 UBIQUITIN-PROTEIN LIGASE SIAH1
Source	Homo sapiens (Human) (SIAH1_HUMAN)

Sequence	A:	ANSVLFPCKYASSGCEITLPHTEKADHEELCEFRPYSCPC PGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDI NLPGAVDWVMMQSCFGFHFMLVLEKQEKHQQFFAIVQLIG TRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMN SDCLVFDTSIAQLFAENGNLGINVTISMC
	B:	ANSVLFPCKYASSGCEITLPHTEKADHEELCEFRPYSCPC PGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDI NLPGAVDWVMMQSCFGFHFMLVLEKQEDGHQQFFAIVQLI GTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIM NSDCLVFDTSIAQLFAENGNLGINVTISMC

Description

Functional site


1)	chain	A
	residue	98
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN A 283
	source	: AC1

2)	chain	A
	residue	105
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN A 283
	source	: AC1

3)	chain	A
	residue	117
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE ZN A 283
	source	: AC1

4)	chain	A
	residue	121
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN A 283
	source	: AC1

5)	chain	A
	residue	128
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN A 284
	source	: AC2

6)	chain	A
	residue	135
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN A 284
	source	: AC2

7)	chain	A
	residue	147
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE ZN A 284
	source	: AC2

8)	chain	A
	residue	152
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE ZN A 284
	source	: AC2

9)	chain	B
	residue	98
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN B 283
	source	: AC3

10)	chain	B
	residue	105
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN B 283
	source	: AC3

11)	chain	B
	residue	117
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE ZN B 283
	source	: AC3

12)	chain	B
	residue	121
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN B 283
	source	: AC3

13)	chain	B
	residue	128
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN B 284
	source	: AC4

14)	chain	B
	residue	135
	type
	sequence	C
	description	BINDING SITE FOR RESIDUE ZN B 284
	source	: AC4

15)	chain	B
	residue	147
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE ZN B 284
	source	: AC4

16)	chain	B
	residue	152
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE ZN B 284
	source	: AC4

17)	chain	A
	residue	124
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE SO4 A 1283
	source	: AC5

18)	chain	A
	residue	126
	type
	sequence	Y
	description	BINDING SITE FOR RESIDUE SO4 A 1283
	source	: AC5

19)	chain	A
	residue	214
	type
	sequence	T
	description	BINDING SITE FOR RESIDUE SO4 A 1283
	source	: AC5

20)	chain	A
	residue	215
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE SO4 A 1283
	source	: AC5

21)	chain	A
	residue	244
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE SO4 A 1284
	source	: AC6

22)	chain	B
	residue	137
	type
	sequence	W
	description	BINDING SITE FOR RESIDUE SO4 A 1284
	source	: AC6

23)	chain	B
	residue	147
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE SO4 A 1284
	source	: AC6

24)	chain	B
	residue	150
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE SO4 A 1284
	source	: AC6

25)	chain	B
	residue	151
	type
	sequence	Q
	description	BINDING SITE FOR RESIDUE SO4 A 1284
	source	: AC6

26)	chain	B
	residue	220
	type
	sequence	N
	description	BINDING SITE FOR RESIDUE SO4 B 1283
	source	: AC7

27)	chain	B
	residue	270
	type
	sequence	N
	description	BINDING SITE FOR RESIDUE SO4 B 1283
	source	: AC7

28)	chain	B
	residue	124
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE SO4 B 1284
	source	: AC8

29)	chain	B
	residue	126
	type
	sequence	Y
	description	BINDING SITE FOR RESIDUE SO4 B 1284
	source	: AC8

30)	chain	B
	residue	214
	type
	sequence	T
	description	BINDING SITE FOR RESIDUE SO4 B 1284
	source	: AC8

31)	chain	B
	residue	215
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE SO4 B 1284
	source	: AC8

32)	chain	B
	residue	244
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE SO4 B 1284
	source	: AC8

33)	chain	A
	residue	151
	type
	sequence	Q
	description	BINDING SITE FOR RESIDUE SO4 A 1285
	source	: AC9

34)	chain	A
	residue	152
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE SO4 A 1285
	source	: AC9

35)	chain	A
	residue	153
	type
	sequence	K
	description	BINDING SITE FOR RESIDUE SO4 A 1285
	source	: AC9

36)	chain	A
	residue	154
	type
	sequence	S
	description	BINDING SITE FOR RESIDUE SO4 A 1285
	source	: AC9

37)	chain	B
	residue	100
	type
	sequence	Y
	description	BINDING SITE FOR RESIDUE CL B 1285
	source	: BC2

38)	chain	B
	residue	244
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE CL B 1285
	source	: BC2

39)	chain	A
	residue	232
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE CL A 1286
	source	: BC3

40)	chain	B
	residue	231
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE GOL B 1286
	source	: BC4

41)	chain	A
	residue	246
	type
	sequence	G
	description	BINDING SITE FOR RESIDUE GOL A 1287
	source	: BC5

42)	chain	A
	residue	247
	type
	sequence	I
	description	BINDING SITE FOR RESIDUE GOL A 1287
	source	: BC5

43)	chain	A
	residue	248
	type
	sequence	A
	description	BINDING SITE FOR RESIDUE GOL A 1287
	source	: BC5

44)	chain	B
	residue	149
	type
	sequence	M
	description	BINDING SITE FOR RESIDUE GOL A 1287
	source	: BC5

45)	chain	A
	residue	228
	type
	sequence	N
	description	BINDING SITE FOR RESIDUE GOL A 1288
	source	: BC6

46)	chain	A
	residue	229
	type
	sequence	G
	description	BINDING SITE FOR RESIDUE GOL A 1288
	source	: BC6

47)	chain	A
	residue	230
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE GOL A 1288
	source	: BC6

48)	chain	A
	residue	266
	type
	sequence	L
	description	BINDING SITE FOR RESIDUE GOL A 1288
	source	: BC6

49)	chain	A
	residue	267
	type
	sequence	F
	description	BINDING SITE FOR RESIDUE GOL A 1288
	source	: BC6

50)	chain	A
	residue	268
	type
	sequence	A
	description	BINDING SITE FOR RESIDUE GOL A 1288
	source	: BC6

51)	chain	A
	residue	269
	type
	sequence	E
	description	BINDING SITE FOR RESIDUE GOL A 1288
	source	: BC6

52)	chain	B
	residue	228
	type
	sequence	N
	description	BINDING SITE FOR RESIDUE GOL B 1287
	source	: BC7

53)	chain	B
	residue	230
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE GOL B 1287
	source	: BC7

54)	chain	B
	residue	266
	type
	sequence	L
	description	BINDING SITE FOR RESIDUE GOL B 1287
	source	: BC7

55)	chain	B
	residue	267
	type
	sequence	F
	description	BINDING SITE FOR RESIDUE GOL B 1287
	source	: BC7

56)	chain	B
	residue	268
	type
	sequence	A
	description	BINDING SITE FOR RESIDUE GOL B 1287
	source	: BC7

57)	chain	B
	residue	269
	type
	sequence	E
	description	BINDING SITE FOR RESIDUE GOL B 1287
	source	: BC7

58)	chain	B
	residue	129
	type
	sequence	P
	description	BINDING SITE FOR RESIDUE GOL B 1288
	source	: BC8

59)	chain	B
	residue	246
	type
	sequence	G
	description	BINDING SITE FOR RESIDUE GOL B 1288
	source	: BC8

60)	chain	B
	residue	247
	type
	sequence	I
	description	BINDING SITE FOR RESIDUE GOL B 1288
	source	: BC8

61)	chain	B
	residue	248
	type
	sequence	A
	description	BINDING SITE FOR RESIDUE GOL B 1288
	source	: BC8

62)	chain	A
	residue	231
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE GOL A 1289
	source	: BC9

63)	chain	B
	residue	237
	type
	sequence	E
	description	BINDING SITE FOR RESIDUE GOL A 1289
	source	: BC9

64)	chain	B
	residue	238
	type
	sequence	A
	description	BINDING SITE FOR RESIDUE GOL A 1289
	source	: BC9

65)	chain	B
	residue	187
	type
	sequence	F
	description	BINDING SITE FOR RESIDUE GOL B 1289
	source	: CC1

66)	chain	B
	residue	217
	type
	sequence	Q
	description	BINDING SITE FOR RESIDUE GOL B 1289
	source	: CC1

67)	chain	B
	residue	220
	type
	sequence	N
	description	BINDING SITE FOR RESIDUE GOL B 1289
	source	: CC1

68)	chain	B
	residue	221
	type
	sequence	F
	description	BINDING SITE FOR RESIDUE GOL B 1289
	source	: CC1

69)	chain	B
	residue	265
	type
	sequence	Q
	description	BINDING SITE FOR RESIDUE GOL B 1289
	source	: CC1

70)	chain	B
	residue	152
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE GOL B 1290
	source	: CC2

71)	chain	B
	residue	153
	type
	sequence	K
	description	BINDING SITE FOR RESIDUE GOL B 1290
	source	: CC2

72)	chain	B
	residue	154
	type
	sequence	S
	description	BINDING SITE FOR RESIDUE GOL B 1290
	source	: CC2

73)	chain	B
	residue	93
	type
	sequence	S
	description	BINDING SITE FOR RESIDUE GOL B 1291
	source	: CC3

74)	chain	B
	residue	94
	type
	sequence	V
	description	BINDING SITE FOR RESIDUE GOL B 1291
	source	: CC3

75)	chain	B
	residue	96
	type
	sequence	F
	description	BINDING SITE FOR RESIDUE GOL B 1291
	source	: CC3

76)	chain	B
	residue	110
	type
	sequence	P
	description	BINDING SITE FOR RESIDUE GOL B 1291
	source	: CC3

77)	chain	B
	residue	111
	type
	sequence	H
	description	BINDING SITE FOR RESIDUE GOL B 1291
	source	: CC3

78)	chain	A
	residue	224
	type
	sequence	R
	description	BINDING SITE FOR RESIDUE GOL A 1290
	source	: CC4

79)	chain	A
	residue	161
	type
	sequence	E
	description	BINDING SITE FOR RESIDUE GOL A 1291
	source	: CC5

80)	chain	A
	residue	185
	type
	sequence	F
	description	BINDING SITE FOR RESIDUE GOL A 1291
	source	: CC5

81)	chain	A
	residue	186
	type
	sequence	G
	description	BINDING SITE FOR RESIDUE GOL A 1291
	source	: CC5

82)	chain	A
	residue	187
	type
	sequence	F
	description	BINDING SITE FOR RESIDUE GOL A 1291
	source	: CC5

83)	chain	A
	residue	168
	type
	sequence	T
	description	BINDING SITE FOR RESIDUE GOL A 1292
	source	: CC6

84)	chain	A
	residue	176
	type
	sequence	V
	description	BINDING SITE FOR RESIDUE GOL A 1292
	source	: CC6

85)	chain	A
	residue	177
	type
	sequence	D
	description	BINDING SITE FOR RESIDUE GOL A 1292
	source	: CC6

86)	chain	A
	residue	178
	type
	sequence	W
	description	BINDING SITE FOR RESIDUE GOL A 1292
	source	: CC6

87)	chain	A
	residue	93-153
	type	ZN_FING
	sequence	SVLFPCKYASSGCEITLPHTEKADHEELCEFRPYSCPCPG ASCKWQGSLDAVMPHLMHQHK
	description	SIAH-type => ECO:0000255\|PROSITE-ProRule:PRU00455
	source	Swiss-Prot : SWS_FT_FI1

88)	chain	B
	residue	93-153
	type	ZN_FING
	sequence	SVLFPCKYASSGCEITLPHTEKADHEELCEFRPYSCPCPG ASCKWQGSLDAVMPHLMHQHK
	description	SIAH-type => ECO:0000255\|PROSITE-ProRule:PRU00455
	source	Swiss-Prot : SWS_FT_FI1

89)	chain	A
	residue	98
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

90)	chain	A
	residue	105
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

91)	chain	A
	residue	117
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

92)	chain	A
	residue	121
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

93)	chain	B
	residue	98
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

94)	chain	B
	residue	105
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

95)	chain	B
	residue	117
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

96)	chain	B
	residue	121
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI2

97)	chain	A
	residue	128
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3

98)	chain	A
	residue	135
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3

99)	chain	A
	residue	147
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3

100)	chain	A
	residue	152
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3

101)	chain	B
	residue	128
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3

102)	chain	B
	residue	135
	type	BINDING
	sequence	C
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3

103)	chain	B
	residue	147
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3

104)	chain	B
	residue	152
	type	BINDING
	sequence	H
	description	BINDING => ECO:0000269\|PubMed:16085652, ECO:0000269\|PubMed:28546513, ECO:0007744\|PDB:2A25, ECO:0007744\|PDB:5WZZ
	source	Swiss-Prot : SWS_FT_FI3