eF-site/Summary 4c51-A

eF-site ID	4c51-A
PDB Code	4c51
Chain	A

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Title	Crystal Structure of the Catalase-Peroxidase (KatG) R418L mutant from Mycobacterium Tuberculosis
Classification	OXIDOREDUCTASE
Compound	CATALASE-PEROXIDASE
Source	(KATG_MYCTU)

Sequence	A:	GHMKYPVEGGGNQDWWPNRLNLKVLHQNPAVADPMGAAFD YAAEVATIDVDALTRDIEEVMTTSQPWWPADYGHYGPLFI RMAWHAAGTYRIHDGRGGAGGGMQRFAPLNSWPDNASLDK ARRLLWPVKKKYGKKLSWADLIVFAGNCALESMGFKTFGF GFGRVDQWEPDEVYWGKEATWLGDERYSGKRDLENPLAAV QMGLIYVNPEGPNGNPDPMAAAVDIRETFRRMAMNDVETA ALIVGGHTFGKTHGAGPADLVGPEPEAAPLEQMGLGWKSS YGTGTGKDAITSGIEVVWTNTPTKWDNSFLEILYGYEWEL TKSPAGAWQYTAKDGAGAGTIPDPFGGPGRSPTMLATDLS LRVDPIYERITRRWLEHPEELADEFAKAWYKLIHLDMGPV ARYLGPLVPKQTLLWQDPVPAVSHDLVGEAEIASLKSQIR ASGLTVSQLVSTAWAAASSFRGSDKRGGANGGRIRLQPQV GWEVNDPDGDLRKVIRTLEEIQESFNSAAPGNIKVSFADL VVLGGCAAIEKAAKAAGHNITVPFTPGRTDASQEQTDVES FAVLEPKADGFRNYLGKGNPLPAEYMLLDKANLLTLSAPE MTVLVGGLRVLGANYKRLPLGVFTEASESLTNDFFVNLLD MGITWEPSPADDGTYQGKDGSGKVKWTGSRVDLVFGSNSE LRALVEVYGADDAQPKFVQDFVAAWDKVMNLDRFDVR

Description

Functional site


1)	chain	A
	residue	270
	type	BINDING
	sequence	H
	description	axial binding residue => ECO:0000269\|PubMed:15231843, ECO:0000269\|PubMed:16566587, ECO:0000269\|PubMed:24185282, ECO:0007744\|PDB:1SJ2, ECO:0007744\|PDB:2CCA, ECO:0007744\|PDB:2CCD, ECO:0007744\|PDB:4C51
	source	Swiss-Prot : SWS_FT_FI3

2)	chain	A
	residue	108
	type	ACT_SITE
	sequence	H
	description	Proton acceptor => ECO:0000255\|HAMAP-Rule:MF_01961
	source	Swiss-Prot : SWS_FT_FI1

3)	chain	A
	residue	321
	type	ACT_SITE
	sequence	W
	description	Tryptophan radical intermediate => ECO:0000269\|PubMed:18052167
	source	Swiss-Prot : SWS_FT_FI2

4)	chain	A
	residue	262-272
	type	prosite
	sequence	TAALIVGGHTF
	description	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. TAALIVGGHTF
	source	prosite : PS00435

5)	chain	A
	residue	99-110
	type	prosite
	sequence	GPLFIRMAWHAA
	description	PEROXIDASE_2 Peroxidases active site signature. GPlfIRMaWHAA
	source	prosite : PS00436

6)	chain	A
	residue	104
	type	SITE
	sequence	R
	description	Transition state stabilizer => ECO:0000255\|HAMAP-Rule:MF_01961
	source	Swiss-Prot : SWS_FT_FI4

7)	chain	A
	residue	107
	type	CROSSLNK
	sequence	W
	description	Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255); alternate => ECO:0000255\|HAMAP-Rule:MF_01961, ECO:0000269\|PubMed:15231843
	source	Swiss-Prot : SWS_FT_FI5

8)	chain	A
	residue	229
	type	CROSSLNK
	sequence	Y
	description	Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107); alternate => ECO:0000255\|HAMAP-Rule:MF_01961, ECO:0000269\|PubMed:15231843
	source	Swiss-Prot : SWS_FT_FI6

9)	chain	A
	residue	255
	type	CROSSLNK
	sequence	M
	description	Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107); alternate => ECO:0000255\|HAMAP-Rule:MF_01961, ECO:0000269\|PubMed:15231843
	source	Swiss-Prot : SWS_FT_FI6