eF-site ID 4aad-EF PDB Code 4aad Chain E, F click to enlarge Title Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Classification HYDROLASE/DNA Compound DNA ENDONUCLEASE I-CREI Source Chlamydomonas reinhardtii (Chlamydomonas smithii) (4AAD) Sequence E:  TCAAAACGTCGTACGACGTTTTGA F:  TCAAAACGTCGTACGACGTTTTGA Description Functional site 1) chain E residue 518 type sequence G description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 2) chain F residue 606 type sequence A description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 3) chain F residue 607 type sequence C description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 4) chain E residue 505 type sequence A description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 5) chain E residue 506 type sequence A description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 6) chain E residue 507 type sequence C description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 7) chain F residue 618 type sequence G description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 8) chain F residue 614 type sequence C description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 9) chain F residue 615 type sequence G description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 10) chain E residue 514 type sequence C description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 11) chain E residue 515 type sequence G description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 12) chain E residue 513 type sequence A description BINDING SITE FOR RESIDUE GOL E 1525 source : AC6 13) chain F residue 616 type sequence A description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7

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