eF-site ID 4aad-ABEF PDB Code 4aad Chain A, B, E, F click to enlarge Title Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Classification HYDROLASE/DNA Compound DNA ENDONUCLEASE I-CREI Source Chlamydomonas reinhardtii (Chlamydomonas smithii) (4AAD) Sequence A:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSNYILSEI KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK FLEVCTWVDQIAALNDSKTRKTTSETVRAVL B:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSNYILSEI KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK FLEVCTWVDQIAALNDSKTRKTTSETVRAVLD E:  TCAAAACGTCGTACGACGTTTTGA F:  TCAAAACGTCGTACGACGTTTTGA Description Functional site 1) chain A residue 28 type sequence K description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 2) chain A residue 42 type sequence T description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 3) chain A residue 68 type sequence R description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 4) chain A residue 79 type sequence S description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 5) chain E residue 518 type sequence G description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 6) chain F residue 606 type sequence A description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 7) chain F residue 607 type sequence C description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 8) chain B residue 82 type sequence K description BINDING SITE FOR RESIDUE GOL B 1154 source : AC2 9) chain B residue 26 type sequence Q description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 10) chain B residue 28 type sequence K description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 11) chain B residue 42 type sequence T description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 12) chain B residue 79 type sequence S description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 13) chain E residue 505 type sequence A description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 14) chain E residue 506 type sequence A description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 15) chain E residue 507 type sequence C description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 16) chain F residue 618 type sequence G description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 17) chain B residue 20 type sequence D description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 18) chain B residue 46 type sequence T description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 19) chain F residue 614 type sequence C description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 20) chain F residue 615 type sequence G description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 21) chain A residue 20 type sequence D description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 22) chain A residue 46 type sequence T description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 23) chain E residue 514 type sequence C description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 24) chain E residue 515 type sequence G description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 25) chain E residue 513 type sequence A description BINDING SITE FOR RESIDUE GOL E 1525 source : AC6 26) chain B residue 19 type sequence G description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 27) chain B residue 21 type sequence G description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 28) chain B residue 22 type sequence S description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 29) chain B residue 98 type sequence K description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 30) chain F residue 616 type sequence A description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 31) chain B residue 107 type sequence K description BINDING SITE FOR RESIDUE GOL B 1158 source : AC8 32) chain B residue 110 type sequence E description BINDING SITE FOR RESIDUE GOL B 1158 source : AC8 33) chain A residue 19 type catalytic sequence G description 233 source MCSA : MCSA1 34) chain A residue 20 type catalytic sequence D description 233 source MCSA : MCSA1 35) chain B residue 19 type catalytic sequence G description 233 source MCSA : MCSA2 36) chain B residue 20 type catalytic sequence D description 233 source MCSA : MCSA2 37) chain A residue 19 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI1 38) chain A residue 20 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI1 39) chain B residue 19 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI1 40) chain B residue 20 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI1

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