eF-site ID 4aad-AB PDB Code 4aad Chain A, B click to enlarge Title Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Classification HYDROLASE/DNA Compound DNA ENDONUCLEASE I-CREI Source Chlamydomonas reinhardtii (Chlamydomonas smithii) (4AAD) Sequence A:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSNYILSEI KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK FLEVCTWVDQIAALNDSKTRKTTSETVRAVL B:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSNYILSEI KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK FLEVCTWVDQIAALNDSKTRKTTSETVRAVLD Description Functional site 1) chain A residue 28 type sequence K description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 2) chain A residue 42 type sequence T description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 3) chain A residue 68 type sequence R description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 4) chain A residue 79 type sequence S description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 5) chain B residue 82 type sequence K description BINDING SITE FOR RESIDUE GOL B 1154 source : AC2 6) chain B residue 26 type sequence Q description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 7) chain B residue 28 type sequence K description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 8) chain B residue 42 type sequence T description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 9) chain B residue 79 type sequence S description BINDING SITE FOR RESIDUE GOL B 1155 source : AC3 10) chain B residue 20 type sequence D description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 11) chain B residue 46 type sequence T description BINDING SITE FOR RESIDUE GOL B 1156 source : AC4 12) chain A residue 20 type sequence D description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 13) chain A residue 46 type sequence T description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 14) chain B residue 19 type sequence G description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 15) chain B residue 21 type sequence G description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 16) chain B residue 22 type sequence S description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 17) chain B residue 98 type sequence K description BINDING SITE FOR RESIDUE GOL B 1157 source : AC7 18) chain B residue 107 type sequence K description BINDING SITE FOR RESIDUE GOL B 1158 source : AC8 19) chain B residue 110 type sequence E description BINDING SITE FOR RESIDUE GOL B 1158 source : AC8 20) chain A residue 19 type catalytic sequence G description 233 source MCSA : MCSA1 21) chain A residue 20 type catalytic sequence D description 233 source MCSA : MCSA1 22) chain B residue 19 type catalytic sequence G description 233 source MCSA : MCSA2 23) chain B residue 20 type catalytic sequence D description 233 source MCSA : MCSA2 24) chain A residue 19 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI1 25) chain A residue 20 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI1 26) chain B residue 19 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI1 27) chain B residue 20 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI1

Display surface Download Links