eF-site ID 4aad-A PDB Code 4aad Chain A click to enlarge Title Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Classification HYDROLASE/DNA Compound DNA ENDONUCLEASE I-CREI Source Chlamydomonas reinhardtii (Chlamydomonas smithii) (4AAD) Sequence A:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSNYILSEI KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK FLEVCTWVDQIAALNDSKTRKTTSETVRAVL Description Functional site 1) chain A residue 28 type sequence K description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 2) chain A residue 42 type sequence T description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 3) chain A residue 68 type sequence R description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 4) chain A residue 79 type sequence S description BINDING SITE FOR RESIDUE GOL F 1625 source : AC1 5) chain A residue 20 type sequence D description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 6) chain A residue 46 type sequence T description BINDING SITE FOR RESIDUE GOL A 1153 source : AC5 7) chain A residue 19 type catalytic sequence G description 233 source MCSA : MCSA1 8) chain A residue 20 type catalytic sequence D description 233 source MCSA : MCSA1 9) chain A residue 19 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI1 10) chain A residue 20 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI1

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