eF-site/Summary 3u30-A

eF-site ID	3u30-A
PDB Code	3u30
Chain	A

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Title	Crystal structure of a linear-specific Ubiquitin fab bound to linear ubiquitin
Classification	IMMUNE SYSTEM
Compound	linear di-ubiquitin
Source	Homo sapiens (Human) (3U30)

Sequence	A:	SHMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPP DQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQ IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQR LIFAGKQLEDGRTLSDYNIQKESTLHLVL

Description

Functional site


1)	chain	A
	residue	48
	type	MOD_RES
	sequence	K
	description	Phosphoserine; by PINK1 => ECO:0000269\|PubMed:24660806, ECO:0000269\|PubMed:24751536, ECO:0000269\|PubMed:24784582, ECO:0000269\|PubMed:25527291
	source	Swiss-Prot : SWS_FT_FI3

2)	chain	A
	residue	37
	type	SITE
	sequence	P
	description	Interacts with activating enzyme
	source	Swiss-Prot : SWS_FT_FI1

3)	chain	A
	residue	55
	type	SITE
	sequence	T
	description	Interacts with activating enzyme
	source	Swiss-Prot : SWS_FT_FI1

4)	chain	A
	residue	51
	type	SITE
	sequence	E
	description	Essential for function
	source	Swiss-Prot : SWS_FT_FI2

5)	chain	A
	residue	46
	type	CROSSLNK
	sequence	A
	description	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:18719106
	source	Swiss-Prot : SWS_FT_FI11

6)	chain	A
	residue	49
	type	MOD_RES
	sequence	Q
	description	(Microbial infection) ADP-ribosylthreonine => ECO:0000269\|PubMed:32330457
	source	Swiss-Prot : SWS_FT_FI4

7)	chain	A
	residue	10
	type	CROSSLNK
	sequence	G
	description	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:15466860
	source	Swiss-Prot : SWS_FT_FI6

8)	chain	A
	residue	59
	type	MOD_RES
	sequence	Y
	description	ADP-ribosylglycine => ECO:0000269\|PubMed:28525742
	source	Swiss-Prot : SWS_FT_FI5

9)	chain	A
	residue	31
	type	CROSSLNK
	sequence	Q
	description	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16543144
	source	Swiss-Prot : SWS_FT_FI10

10)	chain	A
	residue	59
	type	CROSSLNK
	sequence	Y
	description	Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
	source	Swiss-Prot : SWS_FT_FI7

11)	chain	A
	residue	12
	type	CROSSLNK
	sequence	T
	description	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:25752573, ECO:0000269\|PubMed:25752577, ECO:0000269\|PubMed:34239127
	source	Swiss-Prot : SWS_FT_FI8

12)	chain	A
	residue	16
	type	CROSSLNK
	sequence	E
	description	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:25752577
	source	Swiss-Prot : SWS_FT_FI9

13)	chain	A
	residue	27-52
	type	prosite
	sequence	KAKIQDKEGIPPDQQRLIFAGKQLED
	description	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD
	source	prosite : PS00299

14)	chain	A
	residue	103-128
	type	prosite
	sequence	KAKIQDKEGIPPDQQRLIFAGKQLED
	description	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD
	source	prosite : PS00299