eF-site ID 3puh-A PDB Code 3puh Chain A click to enlarge Title Cocaine Esterase, wild-type biologically active dimer Classification HYDROLASE Compound Cocaine esterase Source Rhodococcus sp. (strain MB1 Bresler) (COCE_RHOSM) Sequence A:  GNYSVASNVMVPMRDGVRLAVDLYRPDADGPVPVLLVRNP YDKFDVFAWSTQSTNWLEFVRDGYAVVIQDTRGLFASEGE FVPHVDDEADAEDTLSWILEQAWCDGNVGMFGVSYLGVTQ WQAAVSGVGGLKAIAPSMASADLYRAPWYGPGGALSVEAL LGWSALIGTGLITSRSDARPEDAADFVQLAAILNDVAGAA SVTPLAEQPLLGRLIPWVIDQVVDHPDNDESWQSISLFER LGGLATPALITAGWYDGFVGESLRTFVAVKDNADARLVVG PWSHSNLTGRNADRKFGIAATYPIQEATTMHKAFFDRHLR GETDALAGVPKVRLFVMGIDEWRDETDWPLPDTAYTPFYL GGSGAANTSTGGGTLSTSISGTESADTYLYDPADPVPSLG GTLLFHNGDNGPADQRPIHDRDDVLCYSTEVLTDPVEVTG TVSARLFVSSSAVDTDFTAKLVDVFPDGRAIALCDGIVRM RYRETLVNPTLIEAGEIYEVAIDMLATSNVFLPGHRIMVQ VSSSNFPKYDRNSNTGGVIAREQLEEMCTAVNRIHRGPEH PSHIVLPIIKR Description Functional site 1) chain A residue 197 type sequence N description BINDING SITE FOR RESIDUE GOL A 588 source : AC1 2) chain A residue 199 type sequence V description BINDING SITE FOR RESIDUE GOL A 588 source : AC1 3) chain A residue 408 type sequence F description BINDING SITE FOR RESIDUE GOL A 588 source : AC1 4) chain A residue 409 type sequence H description BINDING SITE FOR RESIDUE GOL A 588 source : AC1 5) chain A residue 410 type sequence N description BINDING SITE FOR RESIDUE GOL A 588 source : AC1 6) chain A residue 554 type sequence V description BINDING SITE FOR RESIDUE GOL A 589 source : AC2 7) chain A residue 556 type sequence R description BINDING SITE FOR RESIDUE GOL A 589 source : AC2 8) chain A residue 296 type sequence D description BINDING SITE FOR RESIDUE SO4 A 591 source : AC4 9) chain A residue 319 type sequence D description BINDING SITE FOR RESIDUE SO4 A 592 source : AC5 10) chain A residue 323 type sequence R description BINDING SITE FOR RESIDUE SO4 A 592 source : AC5 11) chain A residue 325 type sequence E description BINDING SITE FOR RESIDUE SO4 A 592 source : AC5 12) chain A residue 293 type sequence R description BINDING SITE FOR RESIDUE GOL B 588 source : AC6 13) chain A residue 298 type sequence K description BINDING SITE FOR RESIDUE GOL B 588 source : AC6 14) chain A residue 44 type catalytic sequence Y description 465 source MCSA : MCSA1 15) chain A residue 117 type catalytic sequence S description 465 source MCSA : MCSA1 16) chain A residue 118 type catalytic sequence Y description 465 source MCSA : MCSA1 17) chain A residue 166 type catalytic sequence W description 465 source MCSA : MCSA1 18) chain A residue 259 type catalytic sequence D description 465 source MCSA : MCSA1 19) chain A residue 287 type catalytic sequence H description 465 source MCSA : MCSA1 20) chain A residue 44 type BINDING sequence Y description source Swiss-Prot : SWS_FT_FI3 21) chain A residue 118 type BINDING sequence Y description source Swiss-Prot : SWS_FT_FI3 22) chain A residue 117 type ACT_SITE sequence S description Acyl-ester intermediate => ECO:0000269|PubMed:11742345 source Swiss-Prot : SWS_FT_FI1 23) chain A residue 259 type ACT_SITE sequence D description Charge relay system => ECO:0000269|PubMed:11742345 source Swiss-Prot : SWS_FT_FI2 24) chain A residue 287 type ACT_SITE sequence H description Charge relay system => ECO:0000269|PubMed:11742345 source Swiss-Prot : SWS_FT_FI2 25) chain A residue 44 type SITE sequence Y description Probably involved in activating the substrate carbonyl and the acyl enzyme for hydrolysis source Swiss-Prot : SWS_FT_FI4

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