eF-site ID 3ofi-ABCD PDB Code 3ofi Chain A, B, C, D click to enlarge Title Crystal structure of human insulin-degrading enzyme in complex with ubiquitin Classification HYDROLASE Compound Insulin-degrading enzyme Source Homo sapiens (Human) (UBC_HUMAN) Sequence A:  NNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPT TDKSSAALDVHIGSLSDPPNIAGLSHFLQHMLFLGTKKYP KENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGAL DRFAQFFLSPLFDESAKDREVNAVDSEHEKNVMNDAWRLF QLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELL KFHSAYYSSNLMAVVVLGRESLDDLTNLVVKLFSEVENKN VPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDL QKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGG QKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLR AEGPQEWVFQELKDLNAVAFRFKDKERPRGYTSKIAGILH YYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVS KSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKF KLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFK QDDKFFLPKANLNFEFFSPFAYVDPLHSNMAYLYLELLKD SLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILL KKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHA MYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSR LHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQ LVRYREVQLPDRGWFVYQQRNEVHNNSGIEIYYQTDMQST SENMFLELFAQIISEPAFNTLRTKEQLGYIVFSGPRRANG IQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAF QKHIQALAIRRLDKPKKLSAESAKYWGEIISQQYNFDRDN TEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLARE MNLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPH B:  NNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPT TDKSSAALDVHIGSLSDPPNIAGLSHFLQHMLFLGTKKYP KENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGAL DRFAQFFLSPLFDESAKDREVNAVDSEHEKNVMNDAWRLF QLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELL KFHSAYYSSNLMAVVVLGRESLDDLTNLVVKLFSEVENKN VPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDL QKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGG QKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLR AEGPQEWVFQELKDLNAVAFRFKDKERPRGYTSKIAGILH YYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVS KSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKF KLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFK QDDKFFLPKANLNFEFFSPFAYVDPLHSNMAYLYLELLKD SLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILL KKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHA MYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSR LHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQ LVRYREVQLPDRGWFVYQQRNEVHNNSGIEIYYQTDMQST SENMFLELFAQIISEPAFNTLRTKEQLGYIVFSGPRRANG IQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAF QKHIQALAIRRLDKPKKLSAESAKYWGEIISQQYNFDRDN TEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLARE MNLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPH C:  MQI D:  MQI Description Functional site 1) chain A residue 108 type sequence H description BINDING SITE FOR RESIDUE ZN A 2012 source : AC1 2) chain A residue 112 type sequence H description BINDING SITE FOR RESIDUE ZN A 2012 source : AC1 3) chain A residue 189 type sequence E description BINDING SITE FOR RESIDUE ZN A 2012 source : AC1 4) chain A residue 800 type sequence Q description BINDING SITE FOR RESIDUE DIO A 2013 source : AC2 5) chain A residue 840 type sequence A description BINDING SITE FOR RESIDUE DIO A 2013 source : AC2 6) chain A residue 841 type sequence N description BINDING SITE FOR RESIDUE DIO A 2013 source : AC2 7) chain A residue 842 type sequence G description BINDING SITE FOR RESIDUE DIO A 2013 source : AC2 8) chain A residue 913 type sequence S description BINDING SITE FOR RESIDUE DIO A 2013 source : AC2 9) chain A residue 915 type sequence Q description BINDING SITE FOR RESIDUE DIO A 2013 source : AC2 10) chain B residue 108 type sequence H description BINDING SITE FOR RESIDUE ZN B 2012 source : AC3 11) chain B residue 112 type sequence H description BINDING SITE FOR RESIDUE ZN B 2012 source : AC3 12) chain B residue 189 type sequence E description BINDING SITE FOR RESIDUE ZN B 2012 source : AC3 13) chain B residue 205 type sequence E description BINDING SITE FOR RESIDUE DIO B 2013 source : AC4 14) chain B residue 477 type sequence R description BINDING SITE FOR RESIDUE DIO B 2013 source : AC4 15) chain B residue 479 type sequence A description BINDING SITE FOR RESIDUE DIO B 2013 source : AC4 16) chain B residue 800 type sequence Q description BINDING SITE FOR RESIDUE DIO B 2014 source : AC5 17) chain B residue 840 type sequence A description BINDING SITE FOR RESIDUE DIO B 2014 source : AC5 18) chain B residue 841 type sequence N description BINDING SITE FOR RESIDUE DIO B 2014 source : AC5 19) chain B residue 842 type sequence G description BINDING SITE FOR RESIDUE DIO B 2014 source : AC5 20) chain A residue 189 type SITE sequence E description Essential for function source Swiss-Prot : SWS_FT_FI2 21) chain B residue 108 type SITE sequence H description Essential for function source Swiss-Prot : SWS_FT_FI2 22) chain B residue 112 type SITE sequence H description Essential for function source Swiss-Prot : SWS_FT_FI2 23) chain B residue 189 type SITE sequence E description Essential for function source Swiss-Prot : SWS_FT_FI2 24) chain B residue 429 type MOD_RES sequence R description ADP-ribosylglycine => ECO:0000269|PubMed:28525742 source Swiss-Prot : SWS_FT_FI5 25) chain B residue 895 type MOD_RES sequence D description ADP-ribosylglycine => ECO:0000269|PubMed:28525742 source Swiss-Prot : SWS_FT_FI5 26) chain B residue 192 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 source Swiss-Prot : SWS_FT_FI6 27) chain B residue 697 type CROSSLNK sequence K description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 source Swiss-Prot : SWS_FT_FI6

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