eF-site ID 3nhe-AB
PDB Code 3nhe
Chain A, B

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Title High Resolution Structure (1.26A) of USP2a in Complex with Ubiquitin
Classification Hydrolase/protein binding
Compound Ubiquitin carboxyl-terminal hydrolase 2
Source Homo sapiens (Human) (UBIQ_HUMAN)
Sequence A:  AQGLAGLRNLGNTXFMNSILQCLSNTRELRDYCLQRLYMR
DLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQ
IQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKS
NPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSS
LTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLF
TKEDVLDGDEKPTCCRCRGRKRCIKKFSIQRFPKILVLHL
KRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYN
LYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMS
SSQVRTSDAYLLFYELAS
B:  MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Description


Functional site

1) chain A
residue 425
type
sequence C
description BINDING SITE FOR RESIDUE ZN A 1
source : AC1

2) chain A
residue 428
type
sequence C
description BINDING SITE FOR RESIDUE ZN A 1
source : AC1

3) chain A
residue 476
type
sequence C
description BINDING SITE FOR RESIDUE ZN A 1
source : AC1

4) chain A
residue 479
type
sequence C
description BINDING SITE FOR RESIDUE ZN A 1
source : AC1

5) chain B
residue 27-52
type prosite
sequence KAKIQDKEGIPPDQQRLIFAGKQLED
description UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD
source prosite : PS00299

6) chain A
residue 268-283
type prosite
sequence GLRNLGNTXFMNSILQ
description USP_1 Ubiquitin specific protease (USP) domain signature 1. GLrnlGNtCFMNSiLQ
source prosite : PS00972

7) chain A
residue 541-558
type prosite
sequence YNLYAVSNHSGTTMGGHY
description USP_2 Ubiquitin specific protease (USP) domain signature 2. YnLyAVsnHsGttmg..GHY
source prosite : PS00973

8) chain B
residue 54
type SITE
sequence R
description Interacts with activating enzyme
source Swiss-Prot : SWS_FT_FI1

9) chain B
residue 72
type SITE
sequence R
description Interacts with activating enzyme
source Swiss-Prot : SWS_FT_FI1

10) chain B
residue 29
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127
source Swiss-Prot : SWS_FT_FI10

11) chain B
residue 33
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577
source Swiss-Prot : SWS_FT_FI11

12) chain B
residue 63
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106
source Swiss-Prot : SWS_FT_FI12

13) chain B
residue 68
type SITE
sequence H
description Essential for function
source Swiss-Prot : SWS_FT_FI2

14) chain B
residue 65
type MOD_RES
sequence S
description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291
source Swiss-Prot : SWS_FT_FI3

15) chain A
residue 428
type MOD_RES
sequence C
description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291
source Swiss-Prot : SWS_FT_FI3

16) chain A
residue 476
type MOD_RES
sequence C
description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291
source Swiss-Prot : SWS_FT_FI3

17) chain A
residue 479
type MOD_RES
sequence C
description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291
source Swiss-Prot : SWS_FT_FI3

18) chain B
residue 66
type MOD_RES
sequence T
description (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457
source Swiss-Prot : SWS_FT_FI4

19) chain B
residue 76
type MOD_RES
sequence G
description ADP-ribosylglycine => ECO:0000269|PubMed:28525742
source Swiss-Prot : SWS_FT_FI5

20) chain B
residue 6
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603
source Swiss-Prot : SWS_FT_FI6

21) chain B
residue 76
type CROSSLNK
sequence G
description Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
source Swiss-Prot : SWS_FT_FI7

22) chain B
residue 11
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144
source Swiss-Prot : SWS_FT_FI8

23) chain B
residue 48
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144
source Swiss-Prot : SWS_FT_FI8

24) chain B
residue 27
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860
source Swiss-Prot : SWS_FT_FI9


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