eF-site ID 3mfl-ABCMNO PDB Code 3mfl Chain A, B, C, M, N, O click to enlarge Title Axial Ligand Swapping In Double Mutant Maintains Intradiol-cleavage Chemistry in Protocatechuate 3,4-Dioxygenase Classification OXIDOREDUCTASE Compound Protocatechuate 3,4-dioxygenase alpha chain Source Pseudomonas putida (Arthrobacter siderocapsulatus) (PCXB_PSEPU) Sequence A:  PIELLPETPSQTAGPYVHIGLALEAAGNPTRDQEIWNRLA KPDAPGEHILLLGQVYDGNGHLVRDSFLEVWQADANGEYQ DAYNLENAFNSFGRTATTFDAGEWTLHTVKPGVVNNAAGV PMAPHINISLFARGINIHLHTRLYFDDEAQANAKCPVLNL IEQPQRRETLIAKRCEVDGKTAYRFDIRIQGEGETVFFDF B:  PIELLPETPSQTAGPYVHIGLALEAAGNPTRDQEIWNRLA KPDAPGEHILLLGQVYDGNGHLVRDSFLEVWQADANGEYQ DAYNLENAFNSFGRTATTFDAGEWTLHTVKPGVVNNAAGV PMAPHINISLFARGINIHLHTRLYFDDEAQANAKCPVLNL IEQPQRRETLIAKRCEVDGKTAYRFDIRIQGEGETVFFDF C:  PIELLPETPSQTAGPYVHIGLALEAAGNPTRDQEIWNRLA KPDAPGEHILLLGQVYDGNGHLVRDSFLEVWQADANGEYQ DAYNLENAFNSFGRTATTFDAGEWTLHTVKPGVVNNAAGV PMAPHINISLFARGINIHLHTRLYFDDEAQANAKCPVLNL IEQPQRRETLIAKRCEVDGKTAYRFDIRIQGEGETVFFDF M:  PAQDNSRFVIRDRNWHPKALTPDYKTSIARSPRQALVSIP QSISETTGPNFSHLGFGAHDHDLLLNFNNGGLPIGERIIV AGRVVDQYGKPVPNTLVEMWQANAGGRYRHKNDRYLAPLD PNFGGVGRCLTDSDGYYSFRTIKPGPHPWRNGPNDWRPAH IYFGISGPSIATKLITQLYFEGDPLIPMCPIVKSIANPEA VQQLIAKLDMNNANPMDCLAYRFDIVLRGQRKTHFENC N:  PAQDNSRFVIRDRNWHPKALTPDYKTSIARSPRQALVSIP QSISETTGPNFSHLGFGAHDHDLLLNFNNGGLPIGERIIV AGRVVDQYGKPVPNTLVEMWQANAGGRYRHKNDRYLAPLD PNFGGVGRCLTDSDGYYSFRTIKPGPHPWRNGPNDWRPAH IYFGISGPSIATKLITQLYFEGDPLIPMCPIVKSIANPEA VQQLIAKLDMNNANPMDCLAYRFDIVLRGQRKTHFENC O:  PAQDNSRFVIRDRNWHPKALTPDYKTSIARSPRQALVSIP QSISETTGPNFSHLGFGAHDHDLLLNFNNGGLPIGERIIV AGRVVDQYGKPVPNTLVEMWQANAGGRYRHKNDRYLAPLD PNFGGVGRCLTDSDGYYSFRTIKPGPHPWRNGPNDWRPAH IYFGISGPSIATKLITQLYFEGDPLIPMCPIVKSIANPEA VQQLIAKLDMNNANPMDCLAYRFDIVLRGQRKTHFENC Description Functional site 1) chain A residue 37 type sequence N description BINDING SITE FOR RESIDUE SO4 A 201 source : AC1 2) chain A residue 105 type sequence T description BINDING SITE FOR RESIDUE SO4 A 201 source : AC1 3) chain A residue 107 type sequence H description BINDING SITE FOR RESIDUE SO4 A 201 source : AC1 4) chain A residue 169 type sequence T description BINDING SITE FOR RESIDUE GOL A 202 source : AC2 5) chain A residue 171 type sequence I description BINDING SITE FOR RESIDUE GOL A 202 source : AC2 6) chain A residue 184 type sequence R description BINDING SITE FOR RESIDUE GOL A 202 source : AC2 7) chain A residue 185 type sequence F description BINDING SITE FOR RESIDUE GOL A 202 source : AC2 8) chain A residue 186 type sequence D description BINDING SITE FOR RESIDUE GOL A 202 source : AC2 9) chain A residue 188 type sequence R description BINDING SITE FOR RESIDUE GOL A 202 source : AC2 10) chain A residue 38 type sequence R description BINDING SITE FOR RESIDUE GOL A 203 source : AC3 11) chain A residue 39 type sequence L description BINDING SITE FOR RESIDUE GOL A 203 source : AC3 12) chain A residue 41 type sequence K description BINDING SITE FOR RESIDUE GOL A 203 source : AC3 13) chain A residue 85 type sequence L description BINDING SITE FOR RESIDUE GOL A 203 source : AC3 14) chain A residue 87 type sequence N description BINDING SITE FOR RESIDUE GOL A 203 source : AC3 15) chain A residue 88 type sequence A description BINDING SITE FOR RESIDUE GOL A 203 source : AC3 16) chain A residue 90 type sequence N description BINDING SITE FOR RESIDUE GOL A 203 source : AC3 17) chain A residue 164 type sequence P description BINDING SITE FOR RESIDUE BME A 204 source : AC4 18) chain M residue 408 type sequence Y description BINDING SITE FOR RESIDUE FE M 600 source : AC5 19) chain M residue 447 type sequence H description BINDING SITE FOR RESIDUE FE M 600 source : AC5 20) chain M residue 460 type sequence H description BINDING SITE FOR RESIDUE FE M 600 source : AC5 21) chain M residue 462 type sequence Y description BINDING SITE FOR RESIDUE FE M 600 source : AC5 22) chain M residue 383 type sequence R description BINDING SITE FOR RESIDUE CO3 M 1 source : AC6 23) chain A residue 15 type sequence P description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 24) chain A residue 16 type sequence Y description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 25) chain A residue 133 type sequence R description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 26) chain M residue 408 type sequence Y description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 27) chain M residue 447 type sequence H description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 28) chain M residue 448 type sequence P description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 29) chain M residue 449 type sequence W description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 30) chain M residue 457 type sequence R description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 31) chain M residue 460 type sequence H description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 32) chain M residue 462 type sequence Y description BINDING SITE FOR RESIDUE DHY M 539 source : AC7 33) chain B residue 37 type sequence N description BINDING SITE FOR RESIDUE SO4 B 201 source : AC8 34) chain B residue 38 type sequence R description BINDING SITE FOR RESIDUE SO4 B 201 source : AC8 35) chain B residue 105 type sequence T description BINDING SITE FOR RESIDUE SO4 B 201 source : AC8 36) chain B residue 107 type sequence H description BINDING SITE FOR RESIDUE SO4 B 201 source : AC8 37) chain B residue 168 type sequence E description BINDING SITE FOR RESIDUE GOL B 202 source : AC9 38) chain B residue 169 type sequence T description BINDING SITE FOR RESIDUE GOL B 202 source : AC9 39) chain B residue 171 type sequence I description BINDING SITE FOR RESIDUE GOL B 202 source : AC9 40) chain B residue 184 type sequence R description BINDING SITE FOR RESIDUE GOL B 202 source : AC9 41) chain B residue 185 type sequence F description BINDING SITE FOR RESIDUE GOL B 202 source : AC9 42) chain B residue 186 type sequence D description BINDING SITE FOR RESIDUE GOL B 202 source : AC9 43) chain B residue 188 type sequence R description BINDING SITE FOR RESIDUE GOL B 202 source : AC9 44) chain B residue 23 type sequence L description BINDING SITE FOR RESIDUE BME B 203 source : BC1 45) chain B residue 28 type sequence N description BINDING SITE FOR RESIDUE BME B 203 source : BC1 46) chain B residue 29 type sequence P description BINDING SITE FOR RESIDUE BME B 203 source : BC1 47) chain N residue 367 type sequence F description BINDING SITE FOR RESIDUE BME B 203 source : BC1 48) chain B residue 69 type sequence E description BINDING SITE FOR RESIDUE BME B 204 source : BC2 49) chain B residue 127 type sequence N description BINDING SITE FOR RESIDUE BME B 204 source : BC2 50) chain B residue 128 type sequence I description BINDING SITE FOR RESIDUE BME B 204 source : BC2 51) chain B residue 129 type sequence S description BINDING SITE FOR RESIDUE BME B 204 source : BC2 52) chain B residue 140 type sequence H description BINDING SITE FOR RESIDUE BME B 204 source : BC2 53) chain N residue 470 type sequence I description BINDING SITE FOR RESIDUE BME B 204 source : BC2 54) chain N residue 408 type sequence Y description BINDING SITE FOR RESIDUE FE N 600 source : BC3 55) chain N residue 447 type sequence H description BINDING SITE FOR RESIDUE FE N 600 source : BC3 56) chain N residue 460 type sequence H description BINDING SITE FOR RESIDUE FE N 600 source : BC3 57) chain N residue 462 type sequence Y description BINDING SITE FOR RESIDUE FE N 600 source : BC3 58) chain N residue 383 type sequence R description BINDING SITE FOR RESIDUE SO4 N 4 source : BC4 59) chain N residue 434 type sequence D description BINDING SITE FOR RESIDUE SO4 N 4 source : BC4 60) chain N residue 503 type sequence Q description BINDING SITE FOR RESIDUE GOL N 1 source : BC5 61) chain N residue 505 type sequence I description BINDING SITE FOR RESIDUE GOL N 1 source : BC5 62) chain N residue 522 type sequence R description BINDING SITE FOR RESIDUE GOL N 1 source : BC5 63) chain N residue 523 type sequence F description BINDING SITE FOR RESIDUE GOL N 1 source : BC5 64) chain N residue 524 type sequence D description BINDING SITE FOR RESIDUE GOL N 1 source : BC5 65) chain B residue 15 type sequence P description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 66) chain B residue 16 type sequence Y description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 67) chain N residue 408 type sequence Y description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 68) chain N residue 447 type sequence H description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 69) chain N residue 449 type sequence W description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 70) chain N residue 450 type sequence R description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 71) chain N residue 457 type sequence R description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 72) chain N residue 460 type sequence H description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 73) chain N residue 462 type sequence Y description BINDING SITE FOR RESIDUE DHY N 539 source : BC6 74) chain C residue 37 type sequence N description BINDING SITE FOR RESIDUE SO4 C 201 source : BC7 75) chain C residue 105 type sequence T description BINDING SITE FOR RESIDUE SO4 C 201 source : BC7 76) chain C residue 107 type sequence H description BINDING SITE FOR RESIDUE SO4 C 201 source : BC7 77) chain C residue 169 type sequence T description BINDING SITE FOR RESIDUE GOL C 202 source : BC8 78) chain C residue 171 type sequence I description BINDING SITE FOR RESIDUE GOL C 202 source : BC8 79) chain C residue 184 type sequence R description BINDING SITE FOR RESIDUE GOL C 202 source : BC8 80) chain C residue 185 type sequence F description BINDING SITE FOR RESIDUE GOL C 202 source : BC8 81) chain C residue 186 type sequence D description BINDING SITE FOR RESIDUE GOL C 202 source : BC8 82) chain C residue 188 type sequence R description BINDING SITE FOR RESIDUE GOL C 202 source : BC8 83) chain O residue 408 type sequence Y description BINDING SITE FOR RESIDUE FE O 600 source : BC9 84) chain O residue 447 type sequence H description BINDING SITE FOR RESIDUE FE O 600 source : BC9 85) chain O residue 460 type sequence H description BINDING SITE FOR RESIDUE FE O 600 source : BC9 86) chain O residue 462 type sequence Y description BINDING SITE FOR RESIDUE FE O 600 source : BC9 87) chain O residue 503 type sequence Q description BINDING SITE FOR RESIDUE GOL O 1 source : CC1 88) chain O residue 505 type sequence I description BINDING SITE FOR RESIDUE GOL O 1 source : CC1 89) chain O residue 507 type sequence K description BINDING SITE FOR RESIDUE GOL O 1 source : CC1 90) chain O residue 522 type sequence R description BINDING SITE FOR RESIDUE GOL O 1 source : CC1 91) chain O residue 523 type sequence F description BINDING SITE FOR RESIDUE GOL O 1 source : CC1 92) chain O residue 524 type sequence D description BINDING SITE FOR RESIDUE GOL O 1 source : CC1 93) chain C residue 15 type sequence P description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 94) chain C residue 16 type sequence Y description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 95) chain O residue 408 type sequence Y description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 96) chain O residue 447 type sequence H description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 97) chain O residue 449 type sequence W description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 98) chain O residue 450 type sequence R description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 99) chain O residue 457 type sequence R description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 100) chain O residue 460 type sequence H description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 101) chain O residue 462 type sequence Y description BINDING SITE FOR RESIDUE DHY O 540 source : CC2 102) chain M residue 408 type catalytic sequence Y description 936 source MCSA : MCSA1 103) chain M residue 447 type catalytic sequence H description 936 source MCSA : MCSA1 104) chain M residue 457 type catalytic sequence R description 936 source MCSA : MCSA1 105) chain M residue 460 type catalytic sequence H description 936 source MCSA : MCSA1 106) chain M residue 462 type catalytic sequence Y description 936 source MCSA : MCSA1 107) chain N residue 408 type catalytic sequence Y description 936 source MCSA : MCSA2 108) chain N residue 447 type catalytic sequence H description 936 source MCSA : MCSA2 109) chain N residue 457 type catalytic sequence R description 936 source MCSA : MCSA2 110) chain N residue 460 type catalytic sequence H description 936 source MCSA : MCSA2 111) chain N residue 462 type catalytic sequence Y description 936 source MCSA : MCSA2 112) chain O residue 408 type catalytic sequence Y description 936 source MCSA : MCSA3 113) chain O residue 447 type catalytic sequence H description 936 source MCSA : MCSA3 114) chain O residue 457 type catalytic sequence R description 936 source MCSA : MCSA3 115) chain O residue 460 type catalytic sequence H description 936 source MCSA : MCSA3 116) chain O residue 462 type catalytic sequence Y description 936 source MCSA : MCSA3 117) chain M residue 408 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 118) chain O residue 447 type BINDING sequence H description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 119) chain O residue 460 type BINDING sequence H description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 120) chain O residue 462 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 121) chain M residue 447 type BINDING sequence H description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 122) chain M residue 460 type BINDING sequence H description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 123) chain M residue 462 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 124) chain N residue 408 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 125) chain N residue 447 type BINDING sequence H description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 126) chain N residue 460 type BINDING sequence H description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 127) chain N residue 462 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 128) chain O residue 408 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:7990141 source Swiss-Prot : SWS_FT_FI1 129) chain A residue 51-79 type prosite sequence LLGQVYDGNGHLVRDSFLEVWQADANGEY description INTRADIOL_DIOXYGENAS Intradiol ring-cleavage dioxygenases signature. LlGqVydgnGhlVrdsfLEVwqadanGeY source prosite : PS00083 130) chain M residue 380-408 type prosite sequence VAGRVVDQYGKPVPNTLVEMWQANAGGRY description INTRADIOL_DIOXYGENAS Intradiol ring-cleavage dioxygenases signature. LlGqVydgnGhlVrdsfLEVwqadanGeY source prosite : PS00083

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