eF-site ID 3lkz-AB PDB Code 3lkz Chain A, B click to enlarge Title Structural and functional analyses of a conserved hydrophobic pocket of flavivirus methyltransferase Classification VIRAL PROTEIN Compound Non-structural protein 5 Source West Nile virus (WNV) (Q9Q6P4_WNV) Sequence A:  GRTLGEVWKERLNQMTKEEFTRYRKEAIIEVDRSAAKHAR KEGNVTGGHPVSRGTAKLRWLVERRFLEPVGKVIDLGCGR GGWCYYMATQKRVQEVRGYTKGGPGHEEPQLVQSYGWNIV TMKSGVDVFYRPSECCDTLLCDIGESSSSAEVEEHRTIRV LEMVEDWLHRGPREFCVKVLCPYMPKVIEKMELLQRRYGG GLVRNPLSRNSTHEMYWVSRASGNVVHSVNMTSQVLLGRM EKRTWKGPQYEEDVNLGSGTRA B:  GRTLGEVWKERLNQMTKEEFTRYRKEAIIEVDRSAAKHAR KEGNVTGGHPVSRGTAKLRWLVERRFLEPVGKVIDLGCGR GGWCYYMATQKRVQEVRGYTKGGPGHEEPQLVQSYGWNIV TMKSGVDVFYRPSECCDTLLCDIGESSSSAEVEEHRTIRV LEMVEDWLHRGPREFCVKVLCPYMPKVIEKMELLQRRYGG GLVRNPLSRNSTHEMYWVSRASGNVVHSVNMTSQVLLGRM EKRTWKGPQYEEDVNLGSGTRA Description Functional site 1) chain A residue 56 type sequence S description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 2) chain A residue 58 type sequence G description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 3) chain A residue 81 type sequence G description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 4) chain A residue 82 type sequence C description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 5) chain A residue 83 type sequence G description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 6) chain A residue 86 type sequence G description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 7) chain A residue 87 type sequence W description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 8) chain A residue 105 type sequence K description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 9) chain A residue 110 type sequence H description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 10) chain A residue 111 type sequence E description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 11) chain A residue 130 type sequence V description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 12) chain A residue 131 type sequence D description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 13) chain A residue 132 type sequence V description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 14) chain A residue 133 type sequence F description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 15) chain A residue 146 type sequence D description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 16) chain A residue 147 type sequence I description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 17) chain A residue 149 type sequence E description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 18) chain B residue 200 type sequence R description BINDING SITE FOR RESIDUE SFG A 301 source : AC1 19) chain A residue 159 type sequence H description BINDING SITE FOR RESIDUE GOL A 302 source : AC2 20) chain A residue 246 type sequence K description BINDING SITE FOR RESIDUE GOL A 302 source : AC2 21) chain A residue 247 type sequence R description BINDING SITE FOR RESIDUE GOL A 302 source : AC2 22) chain A residue 248 type sequence T description BINDING SITE FOR RESIDUE GOL A 302 source : AC2 23) chain B residue 177 type sequence R description BINDING SITE FOR RESIDUE GOL A 302 source : AC2 24) chain A residue 200 type sequence R description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 25) chain B residue 56 type sequence S description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 26) chain B residue 58 type sequence G description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 27) chain B residue 81 type sequence G description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 28) chain B residue 82 type sequence C description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 29) chain B residue 83 type sequence G description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 30) chain B residue 86 type sequence G description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 31) chain B residue 87 type sequence W description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 32) chain B residue 104 type sequence T description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 33) chain B residue 105 type sequence K description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 34) chain B residue 110 type sequence H description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 35) chain B residue 111 type sequence E description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 36) chain B residue 130 type sequence V description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 37) chain B residue 131 type sequence D description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 38) chain B residue 132 type sequence V description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 39) chain B residue 133 type sequence F description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 40) chain B residue 146 type sequence D description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 41) chain B residue 149 type sequence E description BINDING SITE FOR RESIDUE SFG B 301 source : AC3 42) chain A residue 177 type sequence R description BINDING SITE FOR RESIDUE GOL B 302 source : AC4 43) chain B residue 159 type sequence H description BINDING SITE FOR RESIDUE GOL B 302 source : AC4 44) chain B residue 246 type sequence K description BINDING SITE FOR RESIDUE GOL B 302 source : AC4 45) chain B residue 247 type sequence R description BINDING SITE FOR RESIDUE GOL B 302 source : AC4 46) chain B residue 248 type sequence T description BINDING SITE FOR RESIDUE GOL B 302 source : AC4 47) chain A residue 56 type MOD_RES sequence S description Phosphoserine => ECO:0000250|UniProtKB:P03314 source Swiss-Prot : SWS_FT_FI11 48) chain B residue 56 type MOD_RES sequence S description Phosphoserine => ECO:0000250|UniProtKB:P03314 source Swiss-Prot : SWS_FT_FI11 49) chain A residue 61 type ACT_SITE sequence K description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 50) chain A residue 146 type ACT_SITE sequence D description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 51) chain A residue 182 type ACT_SITE sequence K description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 52) chain A residue 218 type ACT_SITE sequence E description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 53) chain B residue 61 type ACT_SITE sequence K description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 54) chain B residue 146 type ACT_SITE sequence D description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 55) chain B residue 182 type ACT_SITE sequence K description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 56) chain B residue 218 type ACT_SITE sequence E description For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI1 57) chain A residue 16 type SITE sequence L description mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI7 58) chain A residue 19 type SITE sequence M description mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI7 59) chain B residue 16 type SITE sequence L description mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI7 60) chain B residue 19 type SITE sequence M description mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI7 61) chain A residue 24 type SITE sequence F description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI8 62) chain B residue 24 type SITE sequence F description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI8 63) chain A residue 61 type SITE sequence K description Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI9 64) chain A residue 182 type SITE sequence K description Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI9 65) chain A residue 218 type SITE sequence E description Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI9 66) chain B residue 61 type SITE sequence K description Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI9 67) chain B residue 182 type SITE sequence K description Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI9 68) chain B residue 218 type SITE sequence E description Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI9 69) chain A residue 86 type BINDING sequence G description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 70) chain A residue 87 type BINDING sequence W description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 71) chain A residue 131 type BINDING sequence D description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 72) chain A residue 132 type BINDING sequence V description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 73) chain B residue 86 type BINDING sequence G description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 74) chain B residue 87 type BINDING sequence W description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 75) chain B residue 131 type BINDING sequence D description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 76) chain B residue 132 type BINDING sequence V description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 source Swiss-Prot : SWS_FT_FI3 77) chain A residue 104 type BINDING sequence T description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 78) chain A residue 105 type BINDING sequence K description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 79) chain A residue 147 type BINDING sequence I description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 80) chain A residue 220 type BINDING sequence Y description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 81) chain B residue 104 type BINDING sequence T description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 82) chain B residue 105 type BINDING sequence K description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 83) chain B residue 147 type BINDING sequence I description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 84) chain B residue 220 type BINDING sequence Y description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI4 85) chain A residue 111 type BINDING sequence E description BINDING => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI5 86) chain A residue 146 type BINDING sequence D description BINDING => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI5 87) chain B residue 111 type BINDING sequence E description BINDING => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI5 88) chain B residue 146 type BINDING sequence D description BINDING => ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI5 89) chain A residue 13 type SITE sequence K description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 90) chain B residue 150 type SITE sequence S description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 91) chain B residue 213 type SITE sequence R description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 92) chain B residue 215 type SITE sequence S description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 93) chain A residue 17 type SITE sequence N description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 94) chain A residue 28 type SITE sequence R description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 95) chain A residue 150 type SITE sequence S description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 96) chain A residue 213 type SITE sequence R description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 97) chain A residue 215 type SITE sequence S description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 98) chain B residue 13 type SITE sequence K description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 99) chain B residue 17 type SITE sequence N description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 100) chain B residue 28 type SITE sequence R description mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI6 101) chain A residue 146 type SITE sequence D description Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI10 102) chain B residue 146 type SITE sequence D description Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 source Swiss-Prot : SWS_FT_FI10 103) chain A residue 56 type BINDING sequence S description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI2 104) chain B residue 56 type BINDING sequence S description BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:17267492 source Swiss-Prot : SWS_FT_FI2

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