eF-site ID 3jw0-ABCDXY
PDB Code 3jw0
Chain A, B, C, D, X, Y

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Title E2~Ubiquitin-HECT
Classification LIGASE/SIGNALING PROTEIN
Compound Ubiquitin-conjugating enzyme E2 D2
Source Homo sapiens (Human) (UBIQ_HUMAN)
Sequence A:  ASKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPN
DSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSN
GSISLDILRSQWSPALKISKVLLSICSLLCDPNPDDPLVP
EIARIYKTDREKYNRIAREWTQKYAM
B:  ASKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPN
DSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSN
GSISLDILRSQWSPALKISKVLLSICSLLCDPNPDDPLVP
EIARIYKTDREKYNRIAREWTQKYAM
C:  FEFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRR
IMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKE
MFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIG
RVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDS
EYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNG
SEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTEL
LPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYC
PNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFA
ELYGSNGPQLFTIEQWGSPEKLPRAHTSFNRLDLPPYETF
EDLREKLLMAVENAQG
D:  EFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRI
MSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEM
FNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGR
VAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSE
YYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGS
EIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELL
PIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCP
NHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAE
LYGSNGPQLFTIEQWGSPEKLPRAHTSFNRLDLPPYETFE
DLREKLLMAVENAQ
X:  MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Y:  MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Description


Functional site
1) chain X
residue 27-52
type prosite
sequence KAKIQDKEGIPPDQQRLIFAGKQLED
description UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD
source prosite : PS00299
2) chain X
residue 54
type SITE
sequence R
description Interacts with activating enzyme
source Swiss-Prot : SWS_FT_FI1
3) chain X
residue 72
type SITE
sequence R
description Interacts with activating enzyme
source Swiss-Prot : SWS_FT_FI1
4) chain Y
residue 54
type SITE
sequence R
description Interacts with activating enzyme
source Swiss-Prot : SWS_FT_FI1
5) chain Y
residue 72
type SITE
sequence R
description Interacts with activating enzyme
source Swiss-Prot : SWS_FT_FI1
6) chain X
residue 29
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127
source Swiss-Prot : SWS_FT_FI10
7) chain Y
residue 29
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127
source Swiss-Prot : SWS_FT_FI10
8) chain X
residue 33
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577
source Swiss-Prot : SWS_FT_FI11
9) chain Y
residue 33
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577
source Swiss-Prot : SWS_FT_FI11
10) chain X
residue 63
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106
source Swiss-Prot : SWS_FT_FI12
11) chain Y
residue 63
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106
source Swiss-Prot : SWS_FT_FI12
12) chain X
residue 68
type SITE
sequence H
description Essential for function
source Swiss-Prot : SWS_FT_FI2
13) chain Y
residue 68
type SITE
sequence H
description Essential for function
source Swiss-Prot : SWS_FT_FI2
14) chain X
residue 65
type MOD_RES
sequence S
description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291
source Swiss-Prot : SWS_FT_FI3
15) chain Y
residue 65
type MOD_RES
sequence S
description Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291
source Swiss-Prot : SWS_FT_FI3
16) chain X
residue 66
type MOD_RES
sequence T
description (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457
source Swiss-Prot : SWS_FT_FI4
17) chain Y
residue 66
type MOD_RES
sequence T
description (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457
source Swiss-Prot : SWS_FT_FI4
18) chain X
residue 76
type MOD_RES
sequence G
description ADP-ribosylglycine => ECO:0000269|PubMed:28525742
source Swiss-Prot : SWS_FT_FI5
19) chain Y
residue 76
type MOD_RES
sequence G
description ADP-ribosylglycine => ECO:0000269|PubMed:28525742
source Swiss-Prot : SWS_FT_FI5
20) chain X
residue 6
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603
source Swiss-Prot : SWS_FT_FI6
21) chain Y
residue 6
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603
source Swiss-Prot : SWS_FT_FI6
22) chain X
residue 76
type CROSSLNK
sequence G
description Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
source Swiss-Prot : SWS_FT_FI7
23) chain Y
residue 76
type CROSSLNK
sequence G
description Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
source Swiss-Prot : SWS_FT_FI7
24) chain X
residue 11
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144
source Swiss-Prot : SWS_FT_FI8
25) chain X
residue 48
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144
source Swiss-Prot : SWS_FT_FI8
26) chain Y
residue 11
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144
source Swiss-Prot : SWS_FT_FI8
27) chain Y
residue 48
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144
source Swiss-Prot : SWS_FT_FI8
28) chain X
residue 27
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860
source Swiss-Prot : SWS_FT_FI9
29) chain Y
residue 27
type CROSSLNK
sequence K
description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860
source Swiss-Prot : SWS_FT_FI9

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